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| 1 | The Subcellular Localization and Blue-Light- Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thalianaw显示文摘Phototropin 1 (phot1 ) 是为向光性,叶绿体运动,有气孔的洞,叶扩大,并且响应蓝光的太阳的追踪的一个光敏电阻器。有 PHOT1::GFP 的后面的更早的工作(Sakamoto 和布里格斯, 2002 ) ,我们调查了模式细胞并且在 34 d 的 phot1 的 subcellular 本地化 Arabidopsis thalinana 的旧变白的幼苗。是从本国的在上游的序列表示了, PHOT1::GFP 熔化蛋白质在子叶的离开轴心的纸巾并且在胚轴的伸长的区域强烈被表示。它中等在根顶附近在射击 / 根转变地区并且在房间被表示。一个荧光信号在根外皮,根帽子,和根是无法发现的顶端的分裂组织本身。子叶边缘附近的叶肉房间的血浆膜显得一致地标记但是跨墙由最近的房间部门创造了更强烈被标记。单个房间类型标记的模式与房间类型和发展舞台变化。蓝光的处理原因 PHOT1::GFP,均匀地相对开始在血浆膜散布了,与强烈标记的有小斑点的区域和完全缺乏在胚轴延伸区域在外皮的房间观察的 fluorescencea 现象最好的另外的区域重新组织变得进不同马赛克。伴随物与或追随者这个重组,进在所有房间类型的细胞质的 PHOT1::GFP 行动除了警卫房间调查了。它在黑暗在几个小时以后由未辩别出的机制从细胞质消失。它在细胞质的外观也不它在黑暗的最终的消失被翻译禁止者酮环己酰亚胺也不阻止,尽管后者过程被延迟。我们假设那 blue-light-induced phot1 重新本地化调制 blue-light-activated 信号 transduction。 | Ying-Lang Wan William Eisinger David Ehrhardt Ulrich Kubitscheck Frantisek Baluska Winslow Briggs | 2008 | Molecular Plant2008，1，1： | 10 |
| 2 | Microtubules Are Essential for Guard-Cell Function in Vicia and Arabidopsis显示文摘放射状地安排的外皮的微导管是警卫房间的一个突出的特征。表示 GFP 导管素的警卫房间在微导管的外观看了一致变化 stomata 什么时候开了或关门。当 stomata 关门了,警卫房间显示出更少微导管结构,是否由转移导致了到黑暗,骆驼毛的织物,氢过氧化物,或钠氢碳酸盐。警卫房间不知(关上的 stomata ) 在轻处理上在微导管结构和有气孔的孔显示出增加。GFP-EB1,标记加结束成长的微导管,没在有气孔的闭合上在汇编的正结束或速度的数字显示出变化。因为当微导管结构数字衰退了时,加结束和正结束的生长的率成长的数字没变化,微导管不稳定性或重新整理一定为微导管的明显的损失负责。当外皮的微导管变得拆卸,有关上的 stomata 的警卫房间与开的 stomata 比那些显示出更多的 cytosolic GFP 荧光,尽管与在全部的荧光的大网损失。指向微导管的药在 Vicia 和 Arabidopsis 堵住了警卫房间功能。Oryzalin 破坏了警卫房间微导管并且阻止了有气孔的开和 taxol 稳定的警卫房间微导管和推迟的有气孔的闭合。煤气的交换大小显示为荧光灯标签的蛋白质的 transgenes 没破坏正常有气孔的功能。在微导管与我们的禁止者研究相结合的警卫房间的这些动态变化在警卫房间功能为微导管的一个活跃角色提供证据。 | William Eisinger David Ehrhardt Winslow Briggs | 2012 | Molecular Plant2012，5，3： | 9 |
| 3 | Quantitative Changes in Microtubule Distribution Correlate with Guard Cell Function in Arabidopsis显示文摘放射状地安排的外皮的微导管是警卫房间的一个突出的特征。我们观察了与共焦的显微镜学表示 GFP 导管素(GFP-TUA6 ) 的警卫房间并且当时,在微导管的外观发现了可认识的变化开或靠近的 stomata (Eisinger 等, 2012 ) 。在现在的学习,荧光分发的分析作为 stomata 在警卫房间以内在微导管捆的山峰紧张显示出戏剧的增加开。这增加随能被归因于 polymerized 导管素的全部的荧光的增加被相关。邻近的人行道房间没在山峰紧张或综合荧光看类似的变化有气孔的孔什么时候变化了。标注 RFP 的结束绑定蛋白质 1 的成像(EB1 ) 和在一样的房间表示的标注 YFP 的导管素表明有种结束的微导管的数字仍然保持不变,尽管 polymerized 导管素的全部的数量是更高的在比在关上的警卫房间开。一起拿,这些结果显示在微导管的变化穿被相关与并且为正常要求警卫房间功能被变化在聚类的微导管或捆绑描绘的组织。 | William R. Eisinger Viktor Kirik Charlotte Lewis David W. Ehrhardt Winslow R. Briggs | 2012 | Molecular Plant2012，5，3： | 4 |
| 4 | Usefulness of tumor volumetry by magnetic resonance (MR) imaging in assessing response to radiation therapy in carcinoma of the uterine cervix 显示文摘 | Mayr NA Magnotta VA Ehrhardt JC | 1996 | Int J Radiat Oncol Biol Phys1996，35，： | 2 |
| 5 | Gene therapeutic approaches to inhibit hepatitis B virus replication显示文摘Acute and chronic hepatitis B virus(HBV) infections remain to present a major global health problem. The infection can be associated with acute symptomatic or asymptomatic hepatitis which can cause chronic inflammation of the liver and over years this can lead to cirrhosis and the development of hepatocellularcarcinomas. Currently available therapeutics for chronically infected individuals aim at reducing viral replication and to slow down or stop the progression of the disease. Therefore, novel treatment options are needed to efficiently combat and eradicate this disease. Here we provide a state of the art overview of gene therapeutic approaches to inhibit HBV replication. We discuss non-viral and viral approaches which were explored to deliver therapeutic nucleic acids aiming at reducing HBV replication. Types of delivered therapeutic nucleic acids which were studied since many years include antisense oligodeoxynucleotides and antisense RNA, ribozymes and DNAzymes, RNA interference, and external guide sequences. More recently designer nucleases gained increased attention and were exploited to destroy the HBV genome. In addition we mention other strategies to reduce HBV replication based on delivery of DNA encoding dominant negative mutants and DNA vaccination. In combination with available cell culture and animal models for HBV infection, in vitro and in vivo studies can be performed to test efficacy of gene therapeutic approaches. Recent progress but also challenges will be specified and future perspectives will be discussed. This is an exciting time to explore such approaches because recent successes of gene therapeutic strategies in the clinic to treat genetic diseases raise hope to find alternative treatment options for patients chronically infected with HBV. | Maren Gebbing Thorsten Bergmann Eric Schulz Anja Ehrhardt | 2015 | World Journal of Hepatology2015，7，2： | 2 |
| 6 | Discrete Transparent Boundary Conditions for Schrodinger-type Equations for Non-compactly Supported Initial Data 显示文摘 | Ehrhardt M | 2008 | Appl Numer Math2008，58，5： | 1 |
| 7 | Formation of low molecular weight carbonyl compounds by sensitized photochemical decomposition of aliphatic hydro-carbon in seawater 显示文摘 | EHRHARDT M G WEBER R R | 1991 | Frsenirus J Anal Chem1991，339，： | 1 |
| 8 | Development of a specific radioimmunoassay to measurephysiological changes of circulating leptin in cattle and sheep显示文摘 | Ehrhardt R A | 2000 | J Eedocrinol2000，166，3： | 1 |
| 9 | Insights into the mechanism of FTY720 and compatibility with regulatory T cells for the inhibition of graft-versus-host disease (GVHD)显示文摘 | Taylor PA Ehrhardt MJ Lees CJ | | 0，，： | 1 |
| 10 | Experimental study on the dependence of burnout on the operation conditions and physical properties in wastewater incineration 显示文摘 | EHRHARDT K EHRET A LEUCKEL W | 1998 | Symposium (Interna- tional) on Combustion1998，27，1： | 1 |
| 11 | Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cellspecific gene expression显示文摘 | PLESCH G EHRHARDT T MUELLER-ROEBER B | 2001 | Plant2001，28，： | 1 |
| 12 | Discrete Transparent Boundary Conditions for the Schrodinger Equations 显示文摘 | Ehrhardt M Arnold A | 2001 | Riv Mat Univ Parma2001，，6： | 1 |
| 13 | RBL-2H3 cells are an imprecise model for mast cell mediator release显示文摘 | Passante E Ehrhardt C Sheridan H | 2009 | Inflamm Res2009，58，9： | 1 |
| 14 | Subplantation effect in magnetron sputtered superhard boron carbide thin films 显示文摘 | Ulrich S Ehrhardt H Schwan J | 1998 | Diamond and Related Materials1998，6，7： | 1 |
| 15 | New developments in the field of superhard coatings显示文摘 | Ehrhardt H | 1995 | Surf Coat Technol1995，7475，1： | 1 |
| 16 | Growth and metablism of the ovine placenta during mid-gestation显示文摘 | EHRHARDT R A BELL A W | 1995 | Placenta1995，16，： | 1 |
| 17 | Development of pulmonary bronchiolo-alveolar adenocarcinomas in transgenic mice overexpressing marine -myc and epidermal growth fm:tor in alveolar type II pneumocytes 显示文摘 | Ehrhardt A Barrels T Geick A | 2001 | Br J Cancer2001，84，6： | 1 |
| 18 | The influenza A virus NSI protein inhibits activation ofJun N-terminal ki?nase and AP-l transcription factors显示文摘 | Ludwig S Wang XY Ehrhardt C | 2002 | J Virol2002，76，11： | 1 |
| 19 | Insights into the mechanism of FTY720 and compatibility with regulatory T cells for the inhibition of graft-versus-host disease(GVHD)显示文摘 | Taylor PA Ehrhardt M J Lees C J | | 0，，09： | 1 |
| 20 | Fabrication of con-tact holes by rear side laser ablation of polyimide foils for CIGSsolar modules显示文摘 | EHRHARDT M SCHEIT C RAGNOW S | 2013 | Applied Surface Science2013，278，： | 1 |
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