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| 1 | Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons显示文摘AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ileum.METHODS:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal plexus.The experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and calretinin.RESULTS:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR groups.Double labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 receptor.In the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 receptor.In the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D group.The spatial density of calbindin-IR neurons,however,did not differ among the three groups.The submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR neurons.The average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR neurons.CONCLUSION:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrinsic primary afferent,cholinergic secretomotor and vasomotor neurons.Undernutrition affected P2X2 receptor expression in the submucosal plexus,and neuronal and size.These changes were rescued in the re-fed rats. | Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno Edson Aparecido Liberti John Barton Furness Patricia Castelucci | 2010 | World Journal of Gastroenterology2010，16，29： | 2 |
| 2 | Evaluation of condylar displacement in chil?dren with unilateral posterior crossbite, before and after rapid maxillary expansion显示文摘 | Edson I Solange M | 2012 | Roth williams international society of orthodontics2012，4，1： | 1 |
| 3 | Manufacturing and Microstructural Characterization of Sintered Nickel Wicks for Capillary Pumps显示文摘 | Eduardo Con alves Reimbrecht M rcio Celso Fredel Edson Bazzo | 1999 | Materials Research1999，，3： | 1 |
| 4 | Use of paper capsules for cadmium determination in biological samples by solid sampling flame atomic absorption spectrometry显示文摘 | Adilson Ben da Costa Julio Cezar Paz de Mattos Edson Irineu Mü ller | | 0，，： | 1 |
| 5 | Dragreduction induced by flexible and rigid molecules in a turbulent flowinto a rotating cylindrical double gap device comparison betweenpoly (ethylene oxide),polyacrylamide,and xanthan gum显示文摘 | Pereira Anselmo S Andrade Rafhael M Soares Edson J | 2013 | Journalof Non-Newtonian Fluid Mechanics2013，202，： | 1 |
| 6 | Lipoid pneumonia inchildren following aspiration of mineral oil used in the treat-ment of constipation : high - resolution CT findings in 17 pa-tients 显示文摘 | Glducia Z Edson M Taisa DG | 2007 | Pediatr Radiol2007，37，： | 1 |
| 7 | Venom apparatus of braconid wasps:comparative ultrastructure of reservoirs and gland fila-ments显示文摘 | EDSON K M BARLIN M R VINSON S B | 1982 | Toxicon1982，20，3： | 1 |
| 8 | Beta-tryptase measurements post-mortem in anaphylactic death and in controls显示文摘 | Edson E Van H M | 1998 | Forensic Sci Int1998，93，23： | 1 |
| 9 | Textile sludge applica-tion to non-productive soil:Physico-chemical and phytotoxicity aspects 显示文摘 | Edson V C R Luciana M Maria M S | 2007 | Ecotoxicology and Environmental Safety2007，65，1： | 1 |
| 10 | Production of hydrogen via steam reforming of biofuels on Ni/CeO 2-Al 2 O 3 catalysts promoted by noble metals显示文摘 | Profeti Luciene P R Ticianelli Edson A Assaf Elisabete M | 2009 | International Journal of Hydrogen Energy2009，34，： | 1 |
| 11 | Urinary oligosaecharides:A peripheral marker for Sida carpinifolia exposure or poisoning 显示文摘 | Marisete B Edson M Colodel | 2009 | Toxicon2009，53，： | 1 |
| 12 | Monotone positive solutions for a fourth order equation with nonlinear boundary conditions显示文摘 | EDSON A TO F M MAURICIO L P | 2009 | Nonlinear Analysis2009，71，9： | 1 |
| 13 | Structure and properties of a nanocomposite formed by vanadium pentoxide containing poly(N-propane sulfonic acid aniline)显示文摘 | GIZ M J EDSON A | 2001 | Journal of Power Sources2001，1039，： | 1 |
| 14 | Granulosa cell-expressed BMPR1A and BMPR1B have unique functions in regulating fertility but act redundantly to suppress ovarian tumor development显示文摘 | Edson M A Nalam R L Clementi C | 2010 | Mol Endo- crinol2010，24，6： | 1 |
| 15 | Conglomerated masses of silicosis in sandhlasters: High-resolution CT fndings显示文摘 | EDSON M ANGELA F FLAVIA S | 2006 | European Journal of Radiology2006，59，： | 1 |
| 16 | Conglomerated masses of silicosis in sandblastersz High-resolution CT ndings显示文摘 | EDSON M ANGELA F FLAVIA S | 2006 | European Journal of Radiology2006，59，： | 1 |
| 17 | Analysis of alternariol monomethyl ether on havedo and albedo tissues of tangerines(citrus reticulata)with symptoms of altemaria brown spot 显示文摘 | Rodrigo F M Gezimar D D S Edson R F | 2007 | Journal of Agricultural and Food Chemistry2007，55，13： | 1 |
| 18 | Nickel(II) and zinc(II) removal using Amberlite IR-120 resin: Ion exchange equilibrium and kinetics显示文摘 | Pietro Escobar Franco Márcia Teresinha Veit Carlos Eduardo Borba Gilberto da Cunha Gon?alves Márcia Regina Fagundes-Klen Rosangela Bergamasco Edson Antonio da Silva Pedro Yahico Ramos Suzaki | 2013 | Chemical Engineering Journal2013，，： | 1 |
| 19 | The partial hydrogenation of benzene to cyclohexene by nanoscale ruthenium catalysts in imidazolium ionic liquids显示文摘 | Edson T Silveira Alexandre P Umpierre Liane M Rossi | 2004 | Chemistry: A European Journal2004，10，15： | 1 |
| 20 | Pulmonary infections after bone marrow transplantation: high-resolution CT findings in 111 patients 显示文摘 | Dante LE Emerson LG Edson M | 2005 | A JR2005，185，： | 1 |
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