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| 1 | Updates in the pathophysiological mechanisms of Parkinson's disease: Emerging role of bone marrow mesenchymal stem cells显示文摘AIM: To explore the approaches exerted by mesenchymal stem cells(MSCs) to improve Parkinson's disease(PD) pathophysiology.METHODS: MSCs were harvested from bone marrowof femoral bones of male rats, grown and propagated in culture. Twenty four ovariectomized animals were classified into 3 groups: Group(1) was control, Groups(2) and(3) were subcutaneously administered with rotenone for 14 d after one month of ovariectomy for induction of PD. Then, Group(2) was left untreated, while Group(3) was treated with single intravenous dose of bone marrow derived MSCs(BM-MSCs). SRY gene was assessed by PCR in brain tissue of the female rats. Serum transforming growth factor beta-1(TGF-β1), monocyte chemoattractant protein-1(MCP-1) and brain derived neurotrophic factor(BDNF) levels were assayed by ELISA. Brain dopamine DA level was assayed fluorometrically, while brain tyrosine hydroxylase(TH) and nestin gene expression were detected by semi-quantitative real time PCR. Brain survivin expression was determined by immunohistochemical procedure. Histopathological investigation of brain tissues was also done.RESULTS: BM-MSCs were able to home at the injured brains and elicited significant decrease in serum TGF-β1(489.7 ± 13.0 vs 691.2 ± 8.0, P < 0.05) and MCP-1(89.6 ± 2.0 vs 112.1 ± 1.9, P < 0.05) levels associated with significant increase in serum BDNF(3663 ± 17.8 vs 2905 ± 72.9, P < 0.05) and brain DA(874 ± 15.0 vs 599 ± 9.8, P < 0.05) levels as well as brain TH(1.18 ± 0.004 vs 0.54 ± 0.009, P < 0.05) and nestin(1.29 ± 0.005 vs 0.67 ± 0.006, P < 0.05) genes expression levels. In addition to, producing insignificant increase in the number of positive cells for survivin(293.2 ± 15.9 vs 271.5 ± 15.9, P > 0.05) expression. Finally, the brain sections showed intact histological structure of the striatum as a result of treatment with BM-MSCs. CONCLUSION: The current study sheds light on the therapeutic potential of BM-MSCs against PD pathophysiology via multi-mechanistic actions. | Hanaa H Ahmed Ahmed M Salem Hazem M Atta Emad F Eskandar Abdel Razik H Farrag Mohamed A Ghazy Neveen A Salem Hadeer A Aglan | 2016 | World Journal of Stem Cells2016，8，3： | 9 |
| 2 | Dexamethasone mediates protection against acute pancreatitis via upregulation of pancreatitis-associated proteins显示文摘AIM: To examine the influence of dexamethasone on pancreatitis-associated protein (PAP) gene expression using both in vitro and in vivo models of acute pancreati- tis and to study how PAP gene expression correlates with severity of pancreatitis. METHODS: In vitro, IL-6 stimulated pancreas acinar AR42J cells were cultured with increasing concentrations of dexamethasone and assayed for PAP expression (RT-PCR). In vivo , pancreatitis was induced in rats by retrograde injection of 40 g/L taurocholate into the pancreatic duct. Animals were pretreated with dexamethasone (2 mg/kg) daily or saline for 4 d. Pancreata and serum were harvested after 24 h and gene expression levels of PAPⅠ, Ⅱ and Ⅲ were measured by RT-PCR. Severity of pancreatitis was based on serum amylase, pancreatic wet weight, and histopathological score. RESULTS: In vitro, dexamethasone and IL-6 induced a marked transcription of PAPⅠ, Ⅱ and Ⅲ genes in AR42J cells at 24 h (P < 0.05 for all comparisons). In vivo, pancreas mRNA levels of PAPⅠ, Ⅱ or Ⅲ increased by 2.6-fold, 1.9-fold, and 1.3-fold respectively after dexa- methasone treatment, compared with saline treated ani- mals. Serum amylase levels and edema were significantly lower in the dexamethasone group compared with the saline group. Histopathologic evaluation revealed less inflammation and necrosis in pancreata obtained from dexamethasone treated animals (P < 0.05). CONCLUSION: Dexamethasone significantly decreases the severity of pancreatitis. The protective mechanism ofdexamethasone may be via upregulating PAP gene ex- pression during injury. | Emad Kandil Yin-Yao Lin Martin H Bluth Hong Zhang Gabriel Levi Michael E Zenilman | 2006 | World Journal of Gastroenterology2006，12，42： | 3 |
| 3 | A modified shuffled frog-leaping optimization algorithm: Applications to project management显示文摘 | Emad E Tarek H Donald G | 2007 | Structure and Infrastructure Engineering2007，3，1： | 1 |
| 4 | Advances in the Adomian decomposition method for solving two-point nonlinear boundary value prob- lems with Neumann boundary conditions显示文摘 | EMAD H ABDE E RAN R | 2012 | Compu Math Appl2012，63，6： | 1 |
| 5 | Comparison among five evolutionary-based optimization algorithms显示文摘 | EMAD E TAREK H DONALD G | 2005 | AdvancedEngineering Informatics2005，19，： | 1 |
| 6 | Comparison among Five Evolutionary-based Optimization Algorithms 显示文摘 | EMAD E TAREK H DONALD G | 2005 | Advanced Engineering Informatics2005，19，： | 1 |
| 7 | Modeling of zinc bromide energy storage for vehicular applications 显示文摘 | EMAD M ADEL N RENTEL C H | 2010 | IEEE Transactions on Industrial Electronics2010，57，2： | 1 |
| 8 | A filtering technique to lower LC oscillator phase noise显示文摘 | EMAD H HENRIK S ABIDI A-A | 2001 | IEEE J Sol Sta Circ2001，36，12： | 1 |
| 9 | A modified shuffled frog-leaping opti- mization algorithm: applications to project management显示文摘 | Emad E Tarek H Donald G | 2007 | Structure and Infrastructure Engineering2007，3，1： | 1 |
| 10 | The value of troponin-I measurement in assessment of an thracycline induced cardiotoxicity among breast cancer patients显示文摘 | Lobna S Emad H Hanan S | 2000 | Cancer Inst2000，12，1： | 1 |
| 11 | Comparison among five evolutionary-based optimization algorithms显示文摘 | TAREK H DONALD G | 2005 | Ad-vanced Engineering Informatics2005，19，1： | 1 |
| 12 | Three cases of macrofollic- ular variant of papillary thyroid carcinoma 显示文摘 | Emad R Maha A Kfoury H K | 2011 | Ann Saudi Med2011，31，6： | 1 |
| 13 | Advances in the Adomian decomposition method for solving two-point nonlinear boundary value problems with Neumann boundary conditions显示文摘 | EMAD H ALY ABDE E | 2012 | Compu Math Appl2012，63，6： | 1 |
| 14 | A new relay and jammer selection schemes for secure one-way cooperative networks显示文摘 | DOAA H EMAD S | 2014 | Med- Scientry for wireless personal communications2014，75，1： | 1 |
| 15 | An optimization model for reverse logistics network under stochastic environment using Genetic Algorithm显示文摘 | Mostafa H Emad R | 2012 | International Journal of Business and Social Science2012，3，12： | 1 |
| 16 | A modified shuffled frog-leaping optimization algorithm: Applications to project management 显示文摘 | Emad E Tarek H Donald G | 2007 | Structure and Infrastructure Engineering2007，3，1： | 1 |
| 17 | A New Relay and Jammer Sel- ection Schemes for Secure One-Way Cooperative Netwo- rks显示文摘 | H Doaa Ibrahim S Emad | 2014 | Wireless Personal Communications2014，75，1： | 1 |
| 18 | Comparison of two evolutionary algorithms for optimization of bridge deck repairs显示文摘 | Hatem E Emad E Tarek H Khaled S | 2006 | Computer-Aided Civil and Infrastructure Engineering2006，21，： | 1 |
| 19 | Assessing the quality of web sites 显示文摘 | Layla H Emad A | 2011 | Applied Computing and Informatics2011，9，： | 1 |
| 20 | Comparison of two evolu- tionary algorithms for optimization of bridge deck repairs显示文摘 | Hatem E Emad E Tarek H | 2006 | Computer-Aided Civil and Infrastructure Engineering2006，21，8： | 1 |
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