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| 1 | 秸秆-土壤-旋耕刀系统中秸秆位移仿真分析显示文摘秸秆-土壤-旋耕刀之间的相互作用机理对于分析耕作过程有着重要作用。为研究秸秆-土壤-旋耕刀的宏观及微观相互作用,对系统中的秸秆位移进行了仿真分析。首先建立了秸秆-土壤-旋耕刀相互作用的三维离散元模型;其次,对仿真结果与室内土槽实验结果进行对比分析,在土槽实验中采用示踪器方法测量秸秆位移;仿真中导出每个时刻的秸秆位置用来描述秸秆仿真位移;最后,利用仿真数据对秸秆位移进行三维分析。实验和仿真都在恒定前进速度0.222 m/s和耕深100 mm及4种转速(77、100、123、146 r/min)下进行。实验与仿真结果皆显示秸秆位移随着转速增加呈现增加的趋势;秸秆的水平运动位移总是大于同转速下的侧向位移;高转速下的实验与仿真位移误差约为40%,低转速下为70%。利用拟合的误差方程和仿真值可以估算秸秆的水平和侧向位移值,估算值与实验值误差为8.7%和9.3%。通过追踪不同位置的秸秆颗粒发现:初始位置不同的秸秆颗粒具有不同的运动:位于正切刃内侧的秸秆在刀刃切开土壤时,沿正切刃边缘滑出;而位于侧切刃下方的秸秆在开始时被侧切刃挤压随刀刃一起运动,而后随土壤一起被抛起;远离旋耕刀工作范围的秸秆,则因受土壤扰动影响而仅有微小的位移。紧靠侧切刃及过渡刃周围的秸秆颗粒在考察时间段内被直接旋耕入土。可以利用秸秆的垂直方向受力情况,优化旋耕刀刃口曲线提高秸秆在旋耕过程中直接入土的比例;还可以整合秸秆水平和侧向位移及分布,寻找最优工作参数使秸秆在3个方向都能均匀分散,从而提高秸秆分布均匀度。 | 方会敏 姬长英 Ahmed Ali Tagar 张庆怡 郭俊 | 2016 | 农业机械学报2016，47，1： | 101 |
| 2 | 基于离散元法的旋耕过程土壤运动行为分析显示文摘土壤与耕作部件间的相互作用规律是设计和选用土壤耕作部件的基础。研究土壤和耕作部件间的相互作用规律就是要研究耕作部件对土壤产生的作用和它们之间的作用力,首先必须探讨耕作部件工作时土壤运动规律和施加于土壤的作用力。为此本文建立基于离散元方法的旋耕工作模型;对比分析实验与仿真的土壤位移:在土槽实验中采用示踪块方法测量土壤位移,仿真中通过追踪表层土壤颗粒的运动获得仿真位移;利用实验和仿真数据对土壤位移和运动机理进行分析。结果表明:土壤水平和侧向位移都随着转速增加呈现增加的趋势;土壤的水平运动位移总是大于同转速下的侧向位移。浅层土壤颗粒的运动位移最大,中层土壤次之,深层土壤最小。较深位置的土壤,距离旋转中心越近的土壤颗粒水平位移和侧向位移越大。在旋耕刀切土范围内的土壤,有向相反方向运动趋势的浅、中、深层颗粒比例分别为26.2%、72.1%、48.4%。在水平力作用下,大部分土壤颗粒随着旋耕刀切土有向后运动的行为;土壤在开始时刻的侧向受力和侧向运动方向,由颗粒的侧向位置是否偏离侧切刃轴线决定,位于侧切刃轴线左侧的颗粒,则其侧向力向左,反之亦然;土壤在垂直方向先随着刀具入土向下运动,然后滑出刀刃边界被抛起。本文建立的仿真模型得到的土壤水平位移和侧向位移与相应实验值的误差为24.9%和15.3%。本文运用离散元法进行旋耕过程中土壤宏观和细观运动行为的分析,有助于理解旋耕刀与土壤的相互作用机理,为旋耕机械的设计与优化提供理论依据。 | 方会敏 姬长英 Farman Ali Chandio 郭俊 张庆怡 Chaudhry Arslan | 2016 | 农业机械学报2016，47，3： | 110 |
| 3 | Expression of hepatitis B virus genes in early embryonic cells originated from hamster ova and human spermatozoa transfected with the complete viral genome显示文摘瞄准:在介绍经由在试管内授精(IVF ) 带 HBV DNA 进没有带的仓鼠卵母细胞的能动人的精子以后在早胚胎的房间检测肝炎 B (HBV ) 基因(HB S 和 C 基因) 的表示技术。方法:人的调停精子的 HBV 基因被 IVF 方法交付进没有带的仓鼠卵母细胞。聚合酶链反应(PCR ) 被用来检测在一个房间和二房间的胚胎两个都编码基因的 HB S 和 pre-Core/Core (pre-C/C ) 。反向的 transcription-PCR (RT-PCR ) 分析被用来学习二基因的表示。用杂交探查的全身的 HBV DNA 的荧光原位杂交(鱼) 分析被执行在主人证实病毒的 DNA 的集成胚胎的染色体。结果:HB S 和 pre-C/C 编码基因在一个房间在场、抄录,二房间的胚胎与带 HBV DNA 序列的人的精子从仓鼠卵 IVF 发源。结论:调停精子的 HBV 基因能在早胚胎的房间复制并且表示自己。这些结果提供 HBV DNA 能经由男细菌行垂直地传给下一代的直接证据。 | Bahy Ahmed Ali Tian-Hua Huang Halima-Hassan Salem Qing-Dong Xie | 2006 | Asian Journal of Andrology2006，8，3： | 62 |
| 4 | Hypoxla inducible factor 1 (HIF-1) and cardioprotection显示文摘 | Demet TEKIN Ali D DURSUN Lei XI | 2010 | Acta Pharmacologica Sinica2010，31，9： | 62 |
| 5 | 膈肌超声:一种预测机械通气患者撤机结局的新方法显示文摘目的:评价超声检查膈肌增厚和活动度对机械通气患者撤机结局的预测价值。方法:纳入54例成功通过自主呼吸试验(SBT)的患者进行研究。SBT过程中,采用超声检查评估患者的膈肌活动度、吸气末和呼气末的膈肌厚度(Tdi)以及膈肌增厚分数(DTF%);同时记录常规的撤机参数。撤机后,追踪观察患者48小时。结果:54例患者中,14例(25.9%)患者撤机失败。撤机成功患者的膈肌活动度、吸气末和呼气末Tdi以及DTF%均高于撤机失败的患者,差异有统计学意义(P<0.05)。与撤机成功相关的膈肌指标的临界值为:膈肌活动度≥10.5mm;吸气末Tdi≥21mm;呼气末Tdi≥10.5mm;DTF%≥34.2%。各指标预测撤机成功的敏感性分别为87.5%、77.5%、80.0%和90.0%;特异性分别为71.5%、86.6%、50.0%和64.3%。将膈肌活动度≥10.5mm和吸气末Tdi≥21mm两者结合,预测撤机成功的敏感性降低至64.9%,但特异性升高至100%;浅快呼吸指数(RSBI)<105用于预测撤机成功的敏感性达90.0%,但特异性仅有18.7%。结论:对通过SBT的患者,膈肌活动度和吸气末膈肌厚度的超声评估,是优质的机械通气患者撤机结局的预测指标。因此,推荐考虑使用上述指标和RSBI,以改善撤机结局。 | Shereen Farghaly Ali A.Hasan | 2017 | 中国护理管理2017，17，1： | 61 |
| 6 | Clinical epidemiology and disease burden of nonalcoholic fatty liver disease显示文摘Nonalcoholic fatty liver disease(NAFLD) is defined as the presence of hepatic fat accumulation after the exclusion of other causes of hepatic steatosis, including other causes of liver disease, excessive alcohol consumption, and other conditions that may lead to hepatic steatosis. NAFLD encompasses a broad clinical spectrum ranging from nonalcoholic fatty liver to nonalcoholic steatohepatitis(NASH), advanced fibrosis, cirrhosis, and finally hepatocellular carcinoma(HCC). NAFLD is the most common liver disease in the world and NASH may soon become the most common indication for liver transplantation. Ongoing persistence of obesity with increasing rate of diabetes will increase the prevalence of NAFLD, and as this population ages, many will develop cirrhosis and end-stage liver disease. There has been a general increase in the prevalence of NAFLD, with Asia leading the rise, yet the United States is following closely behind with a rising prevalence from 15% in 2005 to 25% within 5 years. NAFLD is commonly associated with metabolic comorbidities, including obesity, type Ⅱ diabetes, dyslipidemia, and metabolic syndrome. Our understanding of the pathophysiology of NAFLD is constantly evolving. Based on NAFLD subtypes, it has the potential to progress into advanced fibrosis, end-stage liver disease and HCC. The increasing prevalence of NAFLD with advanced fibrosis, is concerning because patients appear toexperience higher liver-related and non-liver-related mortality than the general population. The increased morbidity and mortality, healthcare costs and declining health related quality of life associated with NAFLD makes it a formidable disease, and one that requires more in-depth analysis. | Brandon J Perumpail Muhammad Ali Khan Eric R Yoo George Cholankeril Donghee Kim Aijaz Ahmed | 2017 | World Journal of Gastroenterology2017，23，47： | 70 |
| 7 | 模糊证据权方法在镇沅(老王寨)地区金矿资源评价中的应用显示文摘采用模糊证据权方法和GeoDASGIS技术开展了镇沅(老王寨)及其邻区的金矿资源潜力评价.分别采用GeoDASGIS软件提供的局部奇异性分析技术、S-A异常分解技术、主成分分析技术、证据权、模糊证据权等技术对相关地球化学元素进行了系统的处理和分析.应用主成分分析方法确定了可能的2种不同成矿类型,并采用主成分得分确定了组合异常点,在此基础上分别采用普通证据权和模糊证据权方法编制了成矿后验概率图,圈定了有利成矿地段.对比普通证据权方法与模糊证据权方法所得结果表明,模糊证据权方法可减小图层离散化造成的有用信息损失,提高预测结果精度. | 成秋明 陈志军 Ali Khaled | 2007 | 地球科学(中国地质大学学报)2007，32，2： | 55 |
| 8 | 利用水稻功能基因SSR标记鉴定水稻种质资源显示文摘利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2~ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13~ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。 | 赵勇 杨凯 Akbar Ali Cheema 翁跃进 | 2002 | 中国农业科学2002，35，4： | 57 |
| 9 | Injectable hydrogels for cartilage and bone tissue engineering显示文摘Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed. | Mei Liu Xin Zeng Chao Ma Huan Yi Zeeshan Ali Xianbo Mou Song Li Yan Deng Nongyue He | 2017 | Bone Research2017，5，2： | 54 |
| 10 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 11 | 灯盏花素的抗氧化作用及拮抗硒对大鼠肝脏毒性的研究显示文摘目的 探讨灯盏花素对人类允许的最高摄入量 (UL upperlimitofintake)硒所致毒性的拮抗作用。 方法 采用体外化学发光法测定灯盏花素清除亚硒酸钠 (Na2 SeO3)产生的活性氧自由基 (ROS)的能力、动物模型研究灯盏花素对大鼠肝脏丙二醛 (MDA)含量、抗氧化酶活性及组织病理学损伤的影响。结果 体外实验表明灯盏花素能有效清除由Na2 SeO3产生的ROS。动物实验中补UL硒及灯盏花素组大鼠肝脏MDA含量低于仅补UL硒组 ;超氧化物歧化酶(SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSH Px)活性高于仅补UL硒组 ;肝组织切片显示受损伤程度与仅补UL硒组相比减轻。结论 灯盏花素能有效拮抗UL硒所致的毒性 。 | Ali Abdella 甘璐 刘琼 刘宏 徐辉碧 | 2003 | 中国药理学通报2003，19，1： | 53 |
| 12 | p16 promoter hypermethylation:A useful serum marker for early detection of gastric cancer显示文摘AIM: To determine p16 promoter hypermethylation in gastric tumoral tissue and serum samples, its impact on p16-protein expression, and correlation with clinical and histological features. METHODS: Samples were obtained from 52 histologically confirmed cases of gastric adenocarcinoma. Gastric tissue and serum of 50 age- and sex-matched individuals with normal gastroscopy and biopsy were obtained as control samples. Methylation-specific polymerase chain reaction (MSP) was used to evaluate methylation status of p16 promoter. p16-protein expression was analyzed by immunohistochemical staining on paraffin-embedded sections. RESULTS: Methylation was detected in 44.2% (23/52) of tumoral tissues. 60.9% of them were also methylated in serum, i.e., 26.9% of all patients (14/52). Methylation was not detected in tissue and sera of control samples. p16-protein expression was decreased in 61.5% of cases (32/52), and was significantly associated with promoter hypermethylation (P < 0.001). Methylation was significantly more frequent in higher pathological grades (P < 0.05). Methylation was not associated with other clinicopathological features and environmental factors including H pylori infection and smoking. CONCLUSION: p16 promoter hypermethylation is an important event in gastric carcinogenesis. It is the principle mechanism of p16 gene silencing. It is related to malignant tumor behavior. Detection of DNA methylation in serum may be a biomarker for early detection of gastric cancer. | Mohammad Reza Abbaszadegan Omeed Moaven Hamid Reza Sima Kamran Ghafarzadegan Azadeh A'rabi Mohammad Naser Forghani Hamid Reza Raziee Ali Mashhadinejad Mostafa Jafarzadeh Ehsan Esmaili-Shandiz Ezzat Dadkhah | 2008 | World Journal of Gastroenterology2008，14，13： | 50 |
| 13 | Numerical and experimental direct shear tests for coarse-grained soils显示文摘The presence of particles larger than the permissible dimensions of conventional laboratory specimens causes difficulty in the determination of shear strength of coarse-grained soils. In this research, the influence of particle size on shear strength of coarse-grained soils was investigated by resorting to experimental tests in different scale and numerical simulations based on discrete element method (DEM). Experimental tests on such soil specimens were based on using the techniques designated as 'parallel' and 'scalping' to prepare gradation of samples in view of the limitation of laboratory specimen size. As a second approach, the direct shear test was numerically simulated on assemblies of elliptical particles. The behaviors of samples under experimental and numerical tests are presented and compared, indicating that the modification of sample gradation has a significant influence on the mechanical properties of coarse-grained soils. It is noted that the shear strengths of samples produced by the scalping method are higher than samples by the parallel method. The scalping method for preparing specimens for direct shear test is therefore recommended. The micromechanical behavior of assemblies under direct shear test is also discussed and the effects of stress level on sample behavior are investigated. | Ahad Bagherzadeh-Khalkhali Ali Asghar Mirghasemi | 2009 | Particuology2009，7，1： | 42 |
| 14 | Microdissection testicular sperm extraction: an update显示文摘有非妨碍的精子缺乏(NOA ) 的病人曾经被认为由于在精液的精子的缺席与很少治疗选择不肥沃。在最后二十年, intracytoplasmic 精子注射( ICSI )的来临,和各种各样的阴囊的精子检索技术的申请,包括好针渴望( FNA ),常规阴囊的精子抽取( TESE )和 microdissection 阴囊的精子抽取( micro-TESE )在这组人革命化了治疗。因为有 NOA 的大多数人将在睾丸以内孤立精子发生的区域,研究说明了那个精子能与 NOA 在大多数人被检索,包括 Klinefelter 的症候群(KS ) ,化疗和 cryptorchidism 的优先的历史。Micro-TESE,什么时候在阴囊的功能上与更少手术后的复杂并发症和否定效果与常规 TESE 相比有更高的精子检索率(SRR ) 。在这篇文章,我们将比较精子抽取的不同过程的功效,与 NOA 在人讨论医疗和睾丸激素优化的角色并且描述 micro-TESE 外科的技术。而且,我们将更新我们的全面经验允许为 NOA 的特定的子集在精子检索的预后上建议。 | Ali A Dabaja Peter N Schlegel | 2013 | Asian Journal of Andrology2013，15，1： | 41 |
| 15 | 动脉粥样硬化与炎症的关系及相关治疗药物显示文摘近来研究表明:动脉粥样硬化为血管受损后发生的一种炎症过程,一些已上市或在研的具有抗炎活性的药物亦被发现具有动脉粥样硬化治疗作用。对炎症反应在动脉粥样硬化发生、发展过程中的作用机制,以及动脉粥样硬化的潜在治疗靶点和相关药物的研发情况进行综述,旨在为动脉粥样硬化发病机制的进一步阐明及相关新药的研发提供参考。 | 宋磊 钱之玉 陈真 KAZI Hamid Ali | 2013 | 药学进展2013，37，2： | 46 |
| 16 | The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage显示文摘在应力之上,肿瘤 suppressor p53 的水平显著地被提高,是众所周知的。然而尽管有广泛的研究,包含的内在的机制重要为导致压力的 p53 规定内部播放器仍然不充分被理解。我们人的 lincRNA-RoR (RoR ) 是 p53 的一个强壮的否定管理者的现在的证据。不同于通过 ubiquitin-proteasome 小径引起 p53 降级的 MDM2, RoR 通过和异构的原子 ribonucleoprotein 的直接相互作用压制 p53 翻译我(hnRNP 我) 。重要地,带 hnRNP 的一个 28 底 RoR 序列我有约束力的主题为 p53 压抑必要、足够。我们进一步证明 RoR 禁止调停 p53 的房间周期拘捕和 apoptosis。最后,我们表明 p53 transcriptionally 导致 RoR 表示的一个 RoR-p53 自动调整的反馈环。一起,这些结果建议 RoR-hnRNP I-p53 轴可以组成一个另外的监视网络让房间更好对各种各样的压力作出回应。 | Ali Zhang Nanjiang Zhou Jianguo Huang Qian Liu Koji Fukuda Ding Ma Zhaohui Lu Cunxue Bai Kounosuke Watabe Yin-Yuan Mo | 2013 | Cell Research2013，23，3： | 47 |
| 17 | 浙江天童常绿阔叶林不同演替阶段地表凋落物的C:N:P化学计量特征显示文摘地表凋落物在森林物质循环中起着重要作用,但是目前缺乏对其不同分解层次中碳(C)、氮(N)、磷(P)演替动态的研究。该文以浙江天童常绿阔叶林为研究对象,用空间代替时间序列的方法,通过测定5个演替阶段地表凋落物不同分解层次的凋落物量、有机碳库和氮磷养分库的储量及C:N:P化学计量特征,探讨地表凋落物特征的演替动态。结果表明:1)随着演替的进行,地表凋落物量和有机碳储量呈现下降的趋势。2)在各演替阶段,有机碳含量在各分解层表现出未分解层(L)>半分解层(F)>已分解层(Y)的趋势;有机碳储量均表现为YF>L,且各层氮含量随着演替的进行均趋于升高。4)磷含量在演替中期群落最低,各演替阶段不同分解层的磷含量皆表现为Y>F>L。磷储量的演替趋势不明显。L层磷储量随着演替进行趋于降低。5)随着演替进行,凋落物C:N、C:P和N:P皆趋于下降(p<0.05)。在各分解层之间,C:N和C:P皆表现为Y | 马文济 赵延涛 张晴晴 Ali ARSHAD 史青茹 阎恩荣 | 2014 | 植物生态学报2014，38，8： | 50 |
| 18 | 种植密度对玉米-大豆带状间作下大豆光合、产量及茎秆抗倒的影响显示文摘【目的】阐明玉米-大豆带状间作下大豆植株冠层在不同种植密度下的光环境变化规律,明确种植密度对间作大豆叶片光合特性、产量形成及茎秆抗倒的影响,为构建寡日照地区间作大豆合理群体密度提供理论参考。【方法】本研究以大豆(川豆-16)和玉米(正红-505)为试验材料。采用双因素随机区组设计,主因素为种植方式,设玉米-大豆带状间作和大豆带状单作2个水平,副因素为大豆的3个种植密度(PD1=17株/m^(2),PD2=20株/m^(2),PD3=25株/m^(2)),研究种植密度对间作大豆冠层内部光环境变化、叶片光合特性、植株生长动态、田间倒伏率及产量构成等的影响。【结果】2年结果表明,在玉米-大豆带状间作系统中,大豆生长中后期受高位作物玉米遮荫和自荫性增加的影响,其植株群体冠层内部的光合有效辐射(PAR)、叶面积指数(LAI)、叶片光合能力、分枝数及产量显著降低,但受玉米影响的程度因大豆种植密度的不同而不同。在间作模式下,PD1和PD2处理的大豆植株群体冠层光合有效辐射比PD3处理分别增加了45.4%和24.8%,净光合速率分别增加了46.1%和12.3%,单株有效荚数分别增加了53.2%和27.2%,单株分枝数分别增加了270.4%和140.9%,田间倒伏率分别降低了50.3%和19.3%。相关性分析发现,间作大豆的田间倒伏率与冠层内部光合有效辐射、叶片净光合速率、茎秆抗折力、茎叶干物质比、单株分枝数及单株有效荚数呈显著负相关,与株高、叶面积指数和单株无效荚数呈显著正相关。【结论】在玉米-大豆带状间作模式下,20株/m^(2)的大豆密度(PD2)有利于创造良好的群体冠层内部光环境,降低植株田间大豆倒伏率,增加光合产物积累,从而提高大豆产量。 | 程彬 刘卫国 王莉 许梅 覃思思 卢俊吉 高阳 李淑贤 Ali RAZA 张熠 Irshan AHMAD 敬树忠 刘然金 杨文钰 | 2021 | 中国农业科学2021，54，19： | 42 |
| 19 | Discovery of direct inhibitors of Keap1–Nrf2 protein–protein interaction as potential therapeutic and preventive agents显示文摘The Keap1–Nrf2–ARE pathway is an important antioxidant defense mechanism that protects cells from oxidative stress and the Keap1–Nrf2 protein–protein interaction(PPI) has become an important drug target to upregulate the expression of ARE-controlled cytoprotective oxidative stress response enzymes in the development of therapeutic and preventive agents for a number of diseases and conditions. However, most known Nrf2 activators/ARE inducers are indirect inhibitors of Keap1–Nrf2PPI and they are electrophilic species that act by modifying the sulfhydryl groups of Keap1's cysteine residues. The electrophilicity of these indirect inhibitors may cause 'off-target' side effects by reacting with cysteine residues of other important cellular proteins. Efforts have recently been focused on the development of direct inhibitors of Keap1–Nrf2 PPI. This article reviews these recent research efforts including the development of high throughput screening assays, the discovery of peptide and small molecule direct inhibitors, and the biophysical characterization of the binding of these inhibitors to the target Keap1 Kelch domain protein. These non-covalent direct inhibitors of Keap1–Nrf2 PPI could potentially be developed into effective therapeutic or preventive agents for a variety of diseases and conditions. | Dhulfiqar Ali Abed Melanie Goldstein Haifa Albanyan Huijuan Jin Longqin Hu | 2015 | Acta Pharmaceutica Sinica B2015，5，4： | 46 |
| 20 | Accuracy of urea breath test in Helicobacter pylori infection: Meta-analysis显示文摘AIM: To quantitatively summarize and appraise the available evidence of urea breath test(UBT) use to diagnose Helicobacter pylori(H. pylori) infection in patients with dyspepsia and provide pooled diagnostic accuracy measures.METHODS: We searched MEDLINE, EMBASE, Cochrane library and other databases for studies addressing the value of UBT in the diagnosis of H. pylori infection. We included cross-sectional studies that evaluated the diagnostic accuracy of UBT in adult patients with dyspeptic symptoms. Risk of bias was assessed using QUADAS(Quality Assessment of Diagnostic Accuracy Studies)-2 tool. Diagnostic accuracy measures were pooled using the random-effects model. Subgroup analysis was conducted by UBT type(13C vs 14C) and by measurement technique(Infrared spectrometry vs Isotope Ratio Mass Spectrometry).RESULTS: Out of 1380 studies identified, only 23 met the eligibility criteria. Fourteen studies(61%) evaluated 13 C UBT and 9 studies(39%) evaluated 14 C UBT. There was significant variation in the type of reference standard tests used across studies.Pooled sensitivity was 0.96(95%CI: 0.95-0.97) andpooled specificity was 0.93(95%CI: 0.91-0.94). Likelihood ratio for a positive test was 12 and for a negative test was 0.05 with an area under thecurve of 0.985. Meta-analyses were associated with a significant statistical heterogeneity that remained unexplained after subgroup analysis. The included studies had a moderate risk of bias.CONCLUSION: UBT has high diagnostic accuracy for detecting H. pylori infection in patients with dyspepsia. The reliability of diagnostic meta-analytic estimates however is limited by significant heterogeneity. | Mazen Ferwana Imad Abdulmajeed Ali Alhajiahmed Wedad Madani Belal Firwana Rim Hasan Osama Altayar Paul J Limburg Mohammad Hassan Murad Bandar Knawy | 2015 | World Journal of Gastroenterology2015，21，4： | 45 |
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