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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Performance of two different types of passive samplers for the GC/ECD-FID determination of environmental VOC levels in air显示文摘 | Jutta Begerow Erich Jermann Türkan Keles L. Dunemann | 1999 | Fresenius’ Journal of Analytical Chemistry1999，，4： | 1 |
| 3 | Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map 显示文摘 | Dunemann F Kahnau R Stane I | 1999 | Theor Appl Genet1999，98，67： | 1 |
| 4 | Determination of arsenic species in human urine using HPLC with online photooxidation or microwave- assisted oxidation combined with flow-injection HG-AAS 显示文摘 | Sur R Begerow J Dunemann L | 1999 | Fresenius J Anal Chem1999，363，： | 1 |
| 5 | Determination of physiological palladium and platinum levels in urine using double focusing magnetic sector field ICP- MS显示文摘 | Begerow J Turfeld M Dunemann L | 1997 | Fresenius'' Journal of Analytical Chemistry1997，359，45： | 1 |
| 6 | Identification of lipoxygenase(LOX)genes putatively involved in fruit flavour formation in apple(Malus×domestica)显示文摘 | VOGT J SCHILLER D ULRICH D SCHWAB W DUNEMANN F | 2013 | Tree genetics&genomes2013，9，6： | 1 |
| 7 | Simultaneous determination of Hg (Ⅱ)and alkylated Hg,Pb,and Sn species in human body fluids using SPME-GC/MS-MS显示文摘 | Dunemann L | 1999 | J Anal Chem1999，363，56： | 1 |
| 8 | New horizons in human biomonitoring of environmentally and occupation- ally relevant metals-sector-field ICP-MS versus elec- trothermal AAS显示文摘 | Begerow J Turfeld M Dunemann L | 2000 | J Anal At Spectrotn2000，15，4： | 1 |
| 9 | Influence of redox potential and plant species on the uptake of nickel and cadmium from soils 显示文摘 | Brown P H Dunemann L Sehulz R | 1989 | Zeitschrift for Pflanzenernahrung und Bodenkunde1989，152，1： | 1 |
| 10 | Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map显示文摘 | Dunemann F Kahnau R Stange I | 1999 | Springer-Verlag Heidelberg1999，98，67： | 1 |
| 11 | New horizons in human biomonitoring of environmentally and occupationally relevant metals--sector-field ICP-MS versus electrothermal AAS显示文摘 | Begerow J Turfeld M Dunemann J | 2000 | J Anal At Spectrom2000，15，4： | 1 |
| 12 | Aromatic hydrocarbons in the atmospheric environment: Part I. Indoor versus outdoor sources, the influence of traffic显示文摘 | Elke Ilgen Natascha Karfich Karsten Levsen Jürgen Angerer Peter Schneider Joachim Heinrich H.-Erich Wichmann Lothar Dunemann Jutta Begerow | 2001 | Atmospheric Environment2001，，7： | 1 |
| 13 | Determination of physiicigical platinum leverls in Human using Magnetic Sector Field Inductively Coupled Plasma Mass Spectrometry in Combination With Ultraviolet Photolysis 显示文摘 | Begerow J Turfeld M Dunemann L | 1996 | Journal of Analytical Atomic Spectrometry1996，11，6： | 1 |
| 14 | Performance of two different types of passive samplersfor the GC/ECD-FID determination of environmentalVOC levels in air显示文摘 | Dunemann L Begerow J Jermann E | 1999 | Fresenius Journal of AnalyticalChemistry1999，363，4： | 1 |
| 15 | Analysis of complex leafard flower characters in Rhododendron using a molecular linkage map 显示文摘 | Dunemann F Kahnau R Stange I | 1999 | Springer-verlag Heidelberg1999，98，： | 1 |
| 16 | Genetic relationship in Malus evaluated by RAPD ‘fingerprinting' of cultivars and wild species显示文摘 | Dunemann F Kahnau R Schmidt H | 1994 | Plant Breeding1994，113，2： | 1 |
| 17 | Genetic rela- tionships in Malus evaluated by RAPD 'fingerprinting' of cul- tivars and wild species显示文摘 | DUNEMANN F KAHNAM R SCHMIDT H | 1994 | Plant Breeding1994，113，： | 1 |
| 18 | Functional outcomes following total laryngopharyngectomy 显示文摘 | Iseli T A Agar N J Dunemann C | 2007 | ANZ J Surg2007，77，11： | 1 |
| 19 | QTL mapping of aroma compounds analysed by headspace solid-phase microextraction gas chromatography in the apple progeny ' Discovery'×' Prima' 显示文摘 | DUNEMANN F ULRICH D BOUDICHEVSKAIA A GRAFE C WEBER W E | 2010 | Molecular Breeding2010，23，3： | 1 |
| 20 | Influence of redox potential and plant species on the uptake of nickel and cadmium from soils显示文摘 | Brown P H Dunemann L Schulz R | 1989 | Pflanzenernar Bodenk1989，152，： | 1 |
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