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| 1 | Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model显示文摘AIM: To evaluate the antiviral potency of a new antihepatitis C virus(HCV) antiviral agent targeting the cellular autophagy machinery. METHODS: Non-infected liver slices, obtained from human liver resection and cut in 350 μm-thick slices(2.7 × 106 cells per slice) were infected with cell culture-grown HCV Con1b/C3 supernatant(multiplicity of infection = 0.1) cultivated for up to ten days. HCV infected slices were treated at day 4 post-infection with GNS-396 for 6 d at different concentrations. HCV replication was evaluated by strand-specific real-time quantitative reverse transcription- polymerase chain reaction. The infectivity titers of supernatants were evaluated by foci formation upon inoculation into naive Huh-7.5.1 cells. The cytotoxic effect of the drugs was evaluated by lactate dehydrogenase leakage assays. RESULTS: The antiviral efficacy of a new antiviral drug, GNS-396, an autophagy inhibitor, on HCV infection of adult human liver slices was evidenced in a dosedependent manner. At day 6 post-treatment, GNS-396 EC50 was 158 nmol/L without cytotoxic effect(compared to hydroxychloroquine EC50 = 1.17 μmol/L).CONCLUSION: Our results demonstrated that our ex vivo model is efficient for evaluation the potency of autophagy inhibitors, in particular a new quinoline derivative GNS-396 as antiviral could inhibit HCV infection in a dosedependent manner without cytotoxic effect. | Sylvie Lagaye Sonia Brun Jesintha Gaston Hong Shen Ruzena Stranska Claire Camus Clarisse Dubray Géraldine Rousseau Pierre-Philippe Massault Jerome Courcambeck Firas Bassisi Philippe Halfon Stanislas Pol | 2016 | World Journal of Hepatology2016，8，21： | 5 |
| 2 | Sodium dodecyl Sulfate-polyacrylamide gel electrophoresis and immunoblotling analysis of smooth-lipopolysaccharide heterogeneity among Brucella biovars related to A and M specificities显示文摘 | Garin-Bastuji B Bowden G A dubray G | 1990 | J Clin Microbiol1990，28，10： | 1 |
| 3 | In vitro radiation-induced apoptosis and early response to low-dose radiotherapy in non- Hodgkins lymphomas显示文摘 | Dubray B Breton C Delic J | 1998 | Radiother Oncol1998，46，2： | 1 |
| 4 | Conformal radiotherapy (CRP) planning for lung cancer: analysis of intrathoracic organ motion during extreme phases of breathing显示文摘 | Giraud P De Rycke Y Dubray B | 2001 | Int J RadiatOncol Biol Phys2001，51，4： | 1 |
| 5 | Serial as- sessment of FDG-PET FDG uptake and functional volume during radiotherapy (RT) in patients with non-small cell lung cancer (NSCLC) 显示文摘 | Edet-Sanson A Dubray B Doyeux K | 2012 | Radiother Oncol2012，102，2： | 1 |
| 6 | Hepatocellular careinoma: a review of the surgical approaches to management 显示文摘 | Dubray BJ Jr Chapman WC Anderson CD | 2011 | Mo Med2011，108，3： | 1 |
| 7 | Competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the Brucella melitensis BP26 protein to evaluate antibody responses in infected and B. melitensis Rev.1 vaccinated sheep显示文摘 | Hanane Salih-Alj Debbarh Michel S. Zygmunt Gérard Dubray Axel Cloeckaert | 1996 | Veterinary Microbiology1996，，3： | 1 |
| 8 | Antibody responses in the serum and gut of chicken lines differing in cecal carriage of Salmonella enteritidis显示文摘 | Florence Berthelot-Hérault Florence Mompart Michel S Zygmunt Gérard Dubray Marion Duchet-Suchaux | 2003 | Veterinary Immunology and Immunopathology2003，，1： | 1 |
| 9 | Sodium dodeeyl sulfate-polyacrylamide gel eleetrophoresis and immunoblotting analysis of smooth-lipopolysaeeharide heterogeneity among Brucella biovars related to A and M specifieities显示文摘 | Bastuji B G R Bowden A Dubray G | 1990 | J Clin Microbiol1990，28，10： | 1 |
| 10 | Morphological description of surface structures on strain B41 of bovine enterotoxigenic Escherichia coli bearing both K99 and F41 antigens显示文摘 | Benin A Dubray G | 1988 | J Gen Microbiol1988，134，4： | 1 |
| 11 | Radiation-in- dueed lung damage after thoracic irradiation for Hodgkin's dis- ease, the role of fraetionation显示文摘 | Dubray B Henry-Amar M Meerwaldt JH | 1995 | Radiother Oncol1995，36，3： | 1 |
| 12 | Epidemiology, outcomes and predictors of recovery in childhood encephalitis : a hospital-based study显示文摘 | Dubray K Anglemyer A Labeaud AD | 2013 | Pediatr Infect Dis J2013，32，8： | 1 |
| 13 | Unacceptably high mortality related to measles epidemics in Niger, Nigeria, and Chad显示文摘 | Grais RF Dubray C Gerstl S | 2007 | 医学动物2007，4，1： | 1 |
| 14 | Hepatocellular carcinoma:a review of the surgical approaches to management显示文摘 | DuBray BJ Jr Chapman WC Anderson CD | | 0，，： | 1 |
| 15 | Zika virus outbreak on Yap Island,Federated States of Micronesia显示文摘 | Duffy MR Chen TH Hancock WT Powers AM Kool JL Lanciotti RS Pretrick M Marfel M Holzbauer S Dubray C Guillaumot L Griggs A Bel M Lambert AJ Laven J Kosoy O Panella A Biggerstaff BJ Fischer M Hayes EB | 2009 | N Engl J Med2009，360，24： | 1 |
| 16 | Invasive human pituitary tumors express a pointmutanted alpha-protein kinase-C显示文摘 | Lovy L Dubray C | 1993 | J Clin Endocrinol Metab1993，79，： | 1 |
| 17 | Prognostic value of tumor regression during radiotherapy for head and neck cancer:a prospective study显示文摘 | Jaulerry C Dubray B Brunin F | 1995 | Int J Radiat Oncol Biol Phys1995，33，： | 1 |
| 18 | Areas of high 18F -FDG uptake on preradiotherapy PET/CT identify preferential sites of local re- lapse after chemoradiotherapy for noll -small cell lung cancer显示文摘 | CALAIS J THUREAU S DUBRAY B | 2015 | J Nucl Med2015，56，2： | 1 |
| 19 | In vitro radiation induced apoptosis and early response to low dose radiotherapy in non Hodgkin's lymphomas显示文摘 | BRETON C DELIC J | 1998 | Radiother Oncol1998，46，2： | 1 |
| 20 | Low genotyping error rates in wild ungulate faeces sampled in winter 显示文摘 | Maudet C Luikart G Dubray D | 2004 | Molecular Ecology Notes2004，4，4： | 1 |
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