|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Titanium dioxide induced inflammation in the small intestine显示文摘AIM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (IL)-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P < 0.01); jejunum:NP 908.4 ± 130.3, MP 813.8 ± 103.8 vs 526.6 ± 61.43 (P < 0.05); and ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P < 0.05). In comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines IL-12, IL-4, IL-23, TNF-α, IFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):IL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P < 0.05); IL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P < 0.05); IL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P < 0.05); TNFα: NP 3.71 ± 1.33, MP 5.44 ± 1.67 vs 0.99± 019 (P < 0.05); IFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ± 0.69 (P < 0.05); and TGF-β: NP 780.70 ± 318.50, MP 1409.00 ± 502.20 vs 205.50 ± 63.93 (P < 0.05). CONCLUSION:Our findings indicate that TiO2 particles induce a Th1-mediated inflammatory response in the small bowel in mice. | Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan Dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello Iêda Nishitokukado Carmen Lúcia Ortiz-Agostinho Maria IrmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi | 2012 | World Journal of Gastroenterology2012，18，34： | 8 |
| 2 | Prognostic significance of vascular endothelial growth factor polymorphisms in colorectal cancer patients显示文摘AIM To investigate the associations of the genetic polymor-phisms of vascular endothelial growth factor A(VEGF-A)-1498C>T and-634G>C, with the survival of patients with colorectal cancer(CRC). METHODS A prospective cohort consisting of 131 Brazilians patients consecutively operated on with a curative intention as a result of sporadic colorectal carcinoma was studied. DNA was extracted from peripheral blood and its amplification and allelic discrimination for each genetic polymorphism was performed using the technique of polymerase chain reaction(PCR) in real-time. The real-time PCR technique was used to identify the VEGF-A-1498C>T(rs833031) and-634G>C(rs2010963) polymorphisms. Genotyping was validated for VEGF-A-1498C>T polymorphism in 129 patients and for VEGF-A-634G>C polymorphism in 118 patients. The analysis of association between categorical variables was performed using logistic regression, survival by Kaplan-Meier method and multivariate analysis by the Cox regression method. RESULTS In the univariate analysis there was a significant association(OR = 0.32; P = 0.048) between genotype CC of the VEGF-A-1498C>T polymorphism and the presence of CRC liver metastasis. There was no association between VEGF-A-1498C>T polymorphism and VEGF-A-634G>C polymorphism with further clinical or anatomopathologic variables. The genotype CC of the VEGF-A-1498C>T polymorphism was significantly correlated with the 5-year survival(P = 0.032), but not significant difference(P = 0.27) was obtained with the VEGF-A-634G>C polymorphism with the 5-year survival in the univariate analysis. The genotype CT(HR = 2.79) and CC(HR = 4.67) of the polymorphism VEGF-A-1498C>T and the genotype CC(HR = 3.76) of the polymorphism VEGF-A-634C>G acted as an independent prognostic factor for the risk of death in CRC patients. CONCLUSION The CT and CC genotypes of the VEGF-A-1498C>T and the CC genotype of the VEGF-A-634C>G polymorphisms are prognostic factors of survival in Brazilians patients with sporadic colorectal carcinoma. | Gilmar Ferreira do Espírito Santo Bianca Borsatto Galera Elisabeth Carmen Duarte Elisabeth Suchi Chen Lenuce Azis Amilcar Sabino Damazo Gabriela Tognini Saba Flávia de Sousa Gehrke Ismael Dale Cotrim Guerreiro da Silva Jaques Waisberg | 2017 | World Journal of Gastrointestinal Oncology2017，9，2： | 4 |
| 3 | 年龄对高血压患者血压评估的影响显示文摘 | 张玲玉 叶鹏 Lima NK Duarte PO da Freiria MG Almeida EF Rezende T Machado JV | 2010 | 中华高血压杂志2010，18，11： | 2 |
| 4 | CT-guided cutting needle biopsy of lung lesions—An effective procedure for adequate material and specific diagnose显示文摘 | Marcos Duarte Guimaraes Marcony Queiroz de Andrade Alexandre Calabria da Fonte Rubens Chojniak Jefferson Luiz Gross | 2010 | European Journal of Radiology2010，，3： | 2 |
| 5 | Increased serum interleukin 22 in patients with rheumatoid arthritis and correlation with disease activity显示文摘 | da Rocha LF Duarte AL Dantas AT | 2012 | J Rheumatol2012，39，7： | 1 |
| 6 | Toxicity and removal efficiency of pharmaceutical metabolite clofibric acid by Typha spp. – Potential use for phytoremediation?显示文摘 | Ana V. Dordio Cátia Duarte Margarida Barreiros A.J. Palace Carvalho A.P. Pinto Cristina Teixeira da Costa | 2008 | Bioresource Technology2008，，3： | 1 |
| 7 | Survey of infectious and parasitic diseases in stray cats at the Lisbon Metropolitan Area, Portugal显示文摘 | Ana Duarte Isabel Castro Isabel M. Pereira da Fonseca Virgilio Almeida Luis M. Madeira de Carvalho José Meireles Maria I. Fazendeiro Luis Tavares Yolanda Vaz | 2009 | Journal of Feline Medicine and Surgery2009，，6： | 1 |
| 8 | Metabolic effects of dietary sucrose and fructose in type Ⅱ diabetic subjects显示文摘 | Malerbi DA Paiva ES Duarte AL | 1996 | Diabetes Care1996，19，11： | 1 |
| 9 | Effect of foliar and substrate fertilization on lady palm seedling growth and development显示文摘 | Da Luz P B Patrcia Duarte de 0liveira Paiva P D Tavares A R | | 0，，： | 1 |
| 10 | Adaptation of image cytometry methodology for DNA ploidy analysis of cervical epithelium samples: A pilot study 显示文摘 | MOTTA DUARTE C E CARVALHO C R DA SILVA - FILHO A L | 2014 | Taiwanese Journal of Obstetrics & Gynecology2014，53，2： | 1 |
| 11 | Identifying digital and fractional transfer functions from a frequency response显示文摘 | DUARTE Valerio JOSE Sa da Costa | 2011 | International Journal of Control2011，84，3： | 1 |
| 12 | Tuning of fractional laiD controllers with Ziegler-Nichols-type rules显示文摘 | DUARTE VALRRIO JOSE SA DA COSTA | 2006 | Signal Pro- cessing2006，86，10： | 1 |
| 13 | Consequences of untreated snoring显示文摘 | da Silveira FJ Duarte RL | 2010 | J Bras Pneumol2010，36，2： | 1 |
| 14 | The concomitance of hyper- tension and diabetes exacerbating retinopathy: the role of inflamma- tion and oxidative stress显示文摘 | Duarte DA Silva KC Rosales MA | 2013 | Curr Clin Pharmacol2013，8，4： | 1 |
| 15 | Optical fiber sensor for Hg(11) based on carbon dots显示文摘 | GONCALYES H M R DUARTE A J ESTEVES DA SILVA J C G | 2010 | Biosensors and Bioelectronics2010，26，4： | 1 |
| 16 | Linear model identification of the archimedes wave swing 显示文摘 | PEDRO Beiro DUARTE Valerio Jose Sa da Costa | 2007 | Powereng2007，，： | 1 |
| 17 | The synergy between stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) : molecular basis and clinical relevance 显示文摘 | Duarte RF Franf DA | 2002 | Leuk Lymphoma2002，43，6： | 1 |
| 18 | Ischemia-modified albumin as an oxidative stress biomarker in obesity显示文摘 | Piva SJ Duarte MM Da Cruz IB | | 0，，： | 1 |
| 19 | Evaluation of the effectiveness of laser fluorescence in monitoring in vitro remineralization of incipient caries lesions inprimary teeth 显示文摘 | Mendes FM Nicolau J Duarte DA | 2003 | Caries Res2003，37，： | 1 |
| 20 | Endocytosis of tight junctions caveolin nitrosylation dependent is improved by cocoa via opioid receptor on RPE cells in diabetic conditions显示文摘 | Rosales MA Silva KC Duarte DA | 2014 | Invest Ophthalmol Vis Sci2014，55，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员