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| 1 | Formation of active oxygen species on single-atom Pt catalyst and promoted catalytic oxidation of toluene显示文摘Catalytic oxidation of toluene over noble metal catalysts is a representative reaction for elimination of volatile organic compounds(VOCs).However,to fully understand the activation of molecular oxygen and the role of active oxygen species generated in this reaction is still a challenging target.Herein,MgO nanosheets and single-atom Pt loaded MgO(Pt SA/MgO)nanosheets were synthesized and used as catalysts in toluene oxidation.The activation process of molecular oxygen and oxidation performance on the two catalysts were contrastively investigated.The Pt SA/MgO exhibited significantly enhanced catalytic activity compared to MgO.The oxygen vacancies can be easily generated on the Pt SA/MgO surface,which facilitate the activation of molecular oxygen and the formation of active oxygen species.Based on the experimental data and theoretical calculations,an active oxygen species promoted oxidation mechanism for toluene was proposed.In the presence of H2O,the molecular oxygen is more favorable to be dissociated to generate•OH on the oxygen vacancies of the Pt SA/MgO surface,which is the dominant active oxygen species.We anticipate that this work may shed light on further investigation of t10.1007/s12274-020-2765-1he oxidation mechanism of toluene and other VOCs over noble metal catalysts. | Shunzheng Zhao Yanfeng Wen Xijun Liu Xianyun Pen Fang Lü Fengyu Gao Xizhou Xie Chengcheng Du Honghong Yi Dongjuan Kang Xiaolong Tang | 2020 | Nano Research2020，13，6： | 12 |
| 2 | Microstructural investigation of Nd-rich phase in sintered Nd-Fe-B magnets through electron microscopy显示文摘intergranular 的分发,形态学和结构在 sintered Nd-Fe-B 磁铁的 Nd 富有的阶段通过电子显微镜学被学习。Backscattered 电子(BSE ) 成像表明有各种各样的形态学和尺寸的 Nd 富有的粒子在谷物边界和四角形的 Nd2Fe14B 矩阵的三倍的连接是随机分布式的。通过选择区域电子衍射(SAED ) 在一个系统的倾斜条件下面的分析,大多数 intergranular 与从几百 nanometres 到几 micrometres 的尺寸, Nd 富有的阶段粒子作为集中脸被识别立方(FCC ) 结构。如此的粒子与他们的邻近的 Nd2Fe14B 矩阵谷物拥有了几种近似取向关系,例如(002 )Nd2Fe14B//(200 )FCC_Nd 富有[120 ]Nd2Fe14B//[001 ]FCC_Nd 富有,(002 )Nd2Fe14B//(22 ? 湯洠条敮楴慺楴湯爠癥牥慳? 敢慨楶牯映牯愠湮慥敬 ? 摎 ???? 敆渠湡潣灭獯瑩 ? | FU Xin HAN Xiaolei DU Zhiwei FENG Haibo LI Yanfeng | 2013 | Journal of Rare Earths2013，31，8： | 11 |
| 3 | IL-17A-dependent gut microbiota is essential for regulating diet-induced disorders in mice显示文摘The gut microbiota plays a key role in obesity and related metabolic disorders, and multiple factors including diet, host genotype, and age regulate it. Many studies have examined the contribution of extrinsic factors to the regulation of the gut microbiota, but the importance of the host genetic constitution cannot be ignored. Interleukin 17A(IL-17A), a pro-inflammatory cytokine, is important in the defense against infection and diseases. Here, we investigated the association among IL-17, a high-fat diet(HFD), and the gut microbiota. Mice deficient in IL-17A were resistant to diet-induced obesity and related diseases. Compared with the Ⅱ-17^(-/-)mice, wild-type(WT) mice challenged with HFD showed obvious weight fluctuations, such as those seen in type 2 diabetes, and hematological changes similar to those associated with metabolic syndrome. However, housing WT mice and Ⅱ-17a^(-/-)mice together significantly alleviated these symptoms in the WT mice. A metagenomic analysis of the mouse feces indicated that the microbial community compositions of these two groups differed before HFD feeding. The HFD mediated shifts in the gut microbial compositions, which were associated with the mouse phenotypes.We also identified potentially beneficial and harmful species present during this period, and drew networks of the most abundant species. A functional analysis indicated pathway changes in the WT and Ⅱ-17a^(-/-)mice when fed the HFD. Collectively, these data underscore the importance of the host factor IL-17 A in shaping and regulating the gut microbiota, which conversely, influences the host health. | Yujing Bi Chunxiao Li Lin Liu Jiyuan Zhou Zhengchao Li Huimin Deng Chunyan Wu Yanping Han Yajun Song Yafang Tan Xiaoyi Wang Zongmin Du Yujun Cui Yanfeng Yan Fachao Zhi Guangwei Liu Nan Qin Heping Zhang Ruifu Yang | 2017 | Science Bulletin2017，62，15： | 5 |
| 4 | Blocking of the PD-1/PD-L1 interaction by a novel cyclic peptide inhibitor for cancer immunotherapy显示文摘The interaction of PD-1/PD-L1 allows tumor cells to escape from immune surveillance.Clinical success of the antibody drugs has proven that blockade of PD-1/PD-L1 pathway is a promising strategy for cancer immunotherapy.Here,we developed a cyclic peptide C8 by using Ph.D.-C7 C phage display technology.C8 showed high binding affinity with h PD-1 and could effectively interfere the interaction of PD-1/PD-L1.Furthermore,C8 could stimulate CD8^(+)T cell activation in human peripheral blood mononuclear cells(PBMCs).We also observed that C8 could suppress tumor growth in CT26 and B16-OVA,as well as anti-PD-1 antibody resistant B16 mouse model.CD8^(+)T cells infiltration significantly increased in tumor microenvironment,and IFN-γsecretion by CD8^(+)T cells in draining lymph nodes also increased.Simultaneously,we exploited T cells depletion models and confirmed that C8 exerted anti-tumor effects via activating CD8^(+)T cells dependent manner.The interaction model of C8 with h PD-1 was simulated and confirmed by alanine scanning.In conclusion,C8 shows anti-tumor capability by blockade of PD-1/PD-L1 interaction,and C8 may provide an alternative candidate for cancer immunotherapy. | Wenjie Zhai Xiuman Zhou Mingxia Zhai Wanqiong Li Yunhui Ran Yixuan Sun Jiangfeng Du Wenshan Zhao Lingxiao Xing Yuanming Qi Yanfeng Gao | 2021 | Science China(Life Sciences)2021，64，4： | 5 |
| 5 | High-precision time and frequency measurement method combining time-space conversion and different frequency phase detection显示文摘According to the time&space conversion relations and different frequency phase detection principle,an ultra-high precision time&frequency measurement method is proposed in this paper.The higher accuracy and stability of the speed of light and electromagnetic signals during the transmission in space or a specific medium enable the measurement of short time interval which uses the coincidence detection of signal’s transmission delay in length.The measurement precision better than 10 picoseconds can be easily obtained.The method develops the length vernier utilizing the stability of signal’s transmission delay,minimizes the fuzzy region of phase coincidence between the standard frequency signal and the measured signal,approaches the best phase coincidences and therefore improves the measurement precision which is higher than the precision provided by the traditional methods based on frequency processing.Besides,the method costs less than the traditional methods and can also solve the problem of the measurement of super-high frequency.Experimental results show the method can improve the measurement precision to 10 12/s in the time&frequency domain. | DU BaoQiang WANG YanFeng CUI GuangZhao GUO ShuTing DONG ShaoFeng LIU Dan | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，11： | 4 |
| 6 | Quantized Phase Step Technique Based on Different Frequency Signals and Its Characteristics显示文摘 | DU Baoqiang DONG Shaofeng ZHOU Wei WANG Yanfeng GUO Shuting | 2013 | Chinese Journal of Electronics2013，22，3： | 4 |
| 7 | Food synthetic biology-driven protein supply transition: From animal-derived production to microbial fermentation显示文摘Animal-derived protein production is one of the major traditional protein supply methods,which continues to face increasing challenges to satisfy global needs due to population growth,augmented individual protein consumption,and aggravated environmental pollution.Thus,ensuring a sustainable protein source is a considerable challenge.The emergence and development of food synthetic biology has enabled the establishment of cell factories that effectively synthesize proteins,which is an important way to solve the protein supply problem.This review aims to discuss the existing problems of traditional protein supply and to elucidate the feasibility of synthetic biology in the process of protein synthesis.Moreover,using artificial bioengineered milk and artificial bioengineered eggs as examples,the progress of food protein supply transition based on synthetic biology has been systematically summarized.Additionally,the future of food synthetic biology as a potential source of protein has been also discussed.By strengthening and innovating the application of food synthetic biology technologies,including genetic engineering and high-throughput screening methods,the current limitations of artificial foods for protein synthesis and production should be addressed.Therefore,the development and industrial production of new food resources should be explored to ensure safe,high-quality,and sustainable global protein supply. | Yanfeng Liu Xiaomin Dong Bin Wang Rongzhen Tian Jianghua Li Long Liu Guocheng Du Jian Chen | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 4 |
| 8 | Dispersion behavior of core-shell silica-polymer nanoparticles显示文摘Core-shell silica nanoparticles are superior in modifying surface wetting behavior, enhancing nucleation and growth in crystallization, improvingdispersion of naked nanoparticles, and thus upgrading the overall properties of organic polymers. The dispersion behavior and morphology ofmonodisperse core-shell silica particles in several polymers including polyesters are reviewed and their potential applications are discussed. | Yi Wang Yangchuan Ke Jingzi Li Shouqin Du Yanfeng Xia | 2007 | China Particuology2007，5，4： | 3 |
| 9 | Metabolic engineering of Escherichia coli for the production of Lacto-N-neotetraose(LNnT)显示文摘Lacto-N-neotetraose(LNnT),one of the most important human milk oligosaccharides,can be used as infants’food addi-tives.Nowadays,extraction,chemical and biological synthesis were utilized to obtain LNnT,while these methods still face some problems such as low yield and high cost.The aim of current work is to construct a de novo biosynthesis pathway of LNnT in E.coli K12 MG1655.The lgtA and lgtB were first expressed by a plasmid,resulting in a LNnT titer of 0.04 g/L.To improve the yield of LNnT on substrate lactose,lacZ and lacI were knocked out,and lacY was over-expressed.As a result,the yield of LNnT on lactose increased from 0.01 to 0.09 mol/mol,and the titer of LNnT elevated to 0.41 g/L.In addition,the pathway was regulated using the titer of Lacto-N-triose II(LNTII)as a measure,and obtained a high titer strain of LNnT for 1.04 g/L.Finally,the gene expressions were fine-tuned,the titer of LNnT reached 1.2 g/L,which was 93%higher than the control strain,and the yield on lactose reached 0.28 mol/mol.The engineering strategy of pathway construction and modulation used in this study is applicable to facilitate the microbial production of other metabolites in E.coli. | Wei Zhang Zhenmin Liu Mengyue Gong Nan Li Xueqin Lv Xiaomin Dong Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，3： | 2 |
| 10 | Current advances in design and engineering strategies of industrial enzymes显示文摘Enzymes are considered as functional proteins,also known as biocatalysts,which are required for normal cellular function.The commercial production of enzymes with distinct features always remains the top priority of researchers for industrial biocatalysis.However,the insufficient yield,low stability,low activity,formation of by-products,complex purification pro-cess,and many other factors are challenging for the enzyme production industry.Novel advancements in protein engineering,especially the combination of multidisciplinary techniques including post-translational enzyme modification,structured assisted protein tailoring and computational modeling approaches,open a new horizon to more efficient production of bio-catalysts.In this review,we focus on the development process of enzyme modification design strategies,such as rational design,semi-rational design and de novo design.Selected examples for each strategy,which are particularly useful for novel enzyme design,are given.We hope this review is helpful for the development of biocatalysts for industrial application. | Tanatarov Dinmukhamed Ziyang Huang Yanfeng Liu Xueqin Lv Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 2 |
| 11 | Transcriptomic Response to Yersinia pestis:RIG-I Like Receptor Signaling Response Is Detrimental to the Host against Plague显示文摘Bacterial pathogens have evolved various mechanisms to modulate host immune responses for successful infection.In this study,RNAsequencing technology was used to analyze the responses of human monocytes THP1 to Yersinia pestis infection.Over 6000 genes were differentially expressed over the 12 h infection.Kinetic responses of pathogen recognition receptor signaling pathways,apoptosis,antigen processing,and presentation pathway and coagulation system were analyzed in detail.Among them,RIG-I-like receptor(RLR) signaling pathway,which was established for antiviral defense,was significantly affected.Mice lacking MAVS,the adaptor of the RLR signahng pathway,were less sensitive to infection and exhibited lower IFN-P production,higher Thl-type cytokines IFN-y and IL-12 production,and lower Th2-type cytokines EL-4 and IL-13 production in the serum compared with wild-type mice.Moreover,infection of pathogenic bacteria other than Y.pestis also altered the expression of the RLR pathway,suggesting that the response of RLR pathway to bacterial infection is a universal mechanism. | Zongmin Du Huiying Yang Yafang Tan Guang Tian Qingwen Zhang Yujun Cui Yanfeng Yan Xiaohong Wu Zuyun Chen Shiyang Cao Yujing Bi Yanping Han Xiaoyi Wang Yajun Song Ruifu Yan | 2014 | Journal of Genetics and Genomics2014，41，7： | 2 |
| 12 | Selection of high efficient strain in degradation of phenol and aniline显示文摘 | Huang Yingping Fang Yanfeng Du Xiaochun | 2007 | J Wuhan Univ (Nat Sci Ed)2007，53，6： | 1 |
| 13 | Al-doped ZnO thin films deposited by reactive frequency magnetron sputtering:H2-induced property changes显示文摘 | Du Guotong Sun Yanfeng | 2007 | Materials Chemistry and Physics2007，101，： | 1 |
| 14 | Interactive System for Video Summarization Based on Multimodal Fusion显示文摘Biography videos based on life performances of prominent figures in history aim to describe great mens' life.In this paper,a novel interactive video summarization for biography video based on multimodal fusion is proposed,which is a novel approach of visualizing the specific features for biography video and interacting with video content by taking advantage of the ability of multimodality.In general,a story of movie progresses by dialogues of characters and the subtitles are produced with the basis on the dialogues which contains all the information related to the movie.In this paper,JGibbsLDA is applied to extract key words from subtitles because the biography video consists of different aspects to depict the characters' whole life.In terms of fusing keywords and key-frames,affinity propagation is adopted to calculate the similarity between each key-frame cluster and keywords.Through the method mentioned above,a video summarization is presented based on multimodal fusion which describes video content more completely.In order to reduce the time spent on searching the interest video content and get the relationship between main characters,a kind of map is adopted to visualize video content and interact with video summarization.An experiment is conducted to evaluate video summarization and the results demonstrate that this system can formally facilitate the exploration of video content while improving interaction and finding events of interest efficiently. | Zheng Li Xiaobing Du Cuixia Ma Yanfeng Li Hongan Wang | 2019 | Journal of Beijing Institute of Technology2019，28，1： | 1 |
| 15 | Calixarene-supported hexadysprosium cluster showing single molecule magnet behavior显示文摘The syntheses, crystal structures, magnetic and luminescent properties of three isomorphous compounds [Ln III 6 (μ 4-O) 2 (C4A) 2-(NO 3 ) 2 (HCOO) 2 (CH 3 O) 2 (DMF) 4 (CH 3 OH) 4 ] (Ln = Gd (1), Tb (2) and Dy (3); H 4 C4A = p-tert-butylcalix[4]arene) are reported. These three compounds are featured with the sandwich-like units constructed by two tail-to-tail calixarene molecules and an in-between Ln III 6 octahedron. The Dy III compound exhibits both single molecule magnet behavior and photoluminescence. | BI YanFeng XU GuanCheng LIAO WuPing DU ShangChao DENG RuiPing WANG BingWu | 2012 | Science China Chemistry2012，55，6： | 1 |
| 16 | Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering in Bacillus subtilis显示文摘Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals.However,the existence of bottlenecks in synthetic pathway can seriously affect production efficiency,which is also one of the core issues for metabolic engineers to solve.Therefore,developing an approach for diagnosing potential metabolic bottlenecks in a faster and simpler manner is of great significance to accelerate cell factories construction.The cell-free reaction system based on cell lysates can transfer metabolic reactions from in vivo to in vitro,providing a flexible access to directly change protein and metabolite variables,thus provides a potential solution for rapid identification of bottlenecks.Here,bottleneck diagnosis of the N-acetylneuraminic acid(NeuAc)biosynthesis pathway in industrially important chassis microorganism Bacillus subtilis was performed using cell-free synthesis system.Specifically,a highly efficient B.subtilis cell-free system for NeuAc de novo synthesis was firstly constructed,which had a 305-fold NeuAc synthesis rate than that in vivo and enabled fast pathway dynamics analysis.Next,through the addition of all potential key intermediates in combination with substrate glucose respectively,it was found that insufficient phosphoenolpyruvate supply was one of the NeuAc pathway bottlenecks.Rational in vivo metabolic engineering of NeuAc-producing B.subtilis was further performed to eliminate the bottleneck.By down-regulating the expression level of pyruvate kinase throughout the growth phase or only in the stationary phase using inhibitory Nterminal coding sequences(NCSs)and growth-dependent regulatory NCSs respectively,the maximal NeuAc titer increased 2.0-fold.Our study provides a rapid method for bottleneck diagnosis,which may help to accelerate the cycle of design,build,test and learn cycle for metabolic engineering. | Rongzhen Tian Minghu Wang Jintian Shi Xiaolong Qin Haoyu Guo Xuanjie Jia Jianghua Li Long Liu Guocheng Du Jian Chen Yanfeng Liu | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 1 |
| 17 | Antimicrobial activity against Shigella sonnei and probiotic properties of wild lactobacilli from fermented food显示文摘 | Yingchun Zhang Lanwei Zhang Ming Du Huaxi Yi Chunfeng Guo Yanfeng Tuo Xue Han Jingyan Li Lili Zhang Lin Yang | 2011 | Microbiological Research2011，，1： | 1 |
| 18 | Mierostructure characteristics and mechanical properties of X80 pipeline steels 显示文摘 | Zhou Min Du Linxiu Zhao Yanfeng | 2012 | Journal of Wuhan University of Technology-Mater2012，27，2： | 1 |
| 19 | Microstructure characteristics and mechanical properties of X80 pipeline steels显示文摘 | Min Zhou Linxiu Du Yanfeng Zhao Xianghua Liu | 2012 | Journal of Wuhan University of Technology-Mater Sci Ed2012，，2： | 1 |
| 20 | Corosolic acid in- duces apoptosis through mitochondrial pathway and caspases ac- tivation in human cervix adenocarcinoma HeLa cells 显示文摘 | Yanfeng Xu Ruiliang Ge Juan Du | 2009 | Canc- er Letters2009，283，： | 1 |
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