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| 1 | Potential role of N-carbamoyl glutamate in biosynthesis of arginine and its significance in production of ruminant animals显示文摘Arginine(ARG) exerts many beneficial effects on animal body and enhanced angiogenesis, lactogenesis, which finally leads to the improvement in nitrogen(N) metabolism, reproduction, lactation, immunity and growth. Unfortunately, unprotected ARG will be degraded in the rumen and its price is high, thus feeding rumen-protected ARG seems to be uneconomical. Alternatively, N-carbamoyl glutamate(NCG) is structural analogue of N-acetyl glutamate, cofactor of cabamoyl phosphate synthetase 1, is lower in rumen degradation compared to ARG. Additionally, rumen epithelial and duodenal cells have potentially utilized the NCG for ureagenesis. Supplementation of NCG to high yielding dairy cows increased plasma concentration of ARG and nitric oxide, decreased the plasma ammonia N and improved lactation performance and N utilization. Supplementation of NCG enhanced pregnancy rates in rats, improved litter size and fetal survival rate, thereby improved the reproductive performance of sows. Oral NCG supplementation increases plasma ARG and somatotropin levels, and increased growth rate and muscle protein synthesis in nursing piglets. The NCG is potential a relatively cheaper source of feed additive to offer vital compensation over oral administration of ARG, resulting in improved ruminant animal health and production. In this article, we reviewed the mechanism of ARG biosynthesis by NCG and their significance in growth, reproduction, milk production and N utilization in ruminant animals. | Bahram Chacher Hongyun Liu Diming Wang Jianxin Liu | 2013 | Journal of Animal Science and Biotechnology2013，4，4： | 15 |
| 2 | Effect of dietary phosphorus content on milk production and phosphorus excretion in dairy cows显示文摘Background:Phosphorus(P) supplementation is costly and can result in excess P excretion.This study investigated the effects of reducing dietary P on milk production and P excretion in dairy cows over a full lactation.Method:Forty-five multiparous Holstein dairy cows were divided into 15 blocks according to expected calving date and previous milk yield,and assigned randomly to one of the three dietary treatments:0.37,0.47,and 0.57%P(DM basis);these P levels represent the NRC recommendations,Chinese recommendations,and the amount of dietary P commonly fed by Chinese dairy farmers,respectively.Average daily feed intake was calculated from monthly data on feed offered and refused.Milk yields of individual cows were recorded weekly,and milk samples were taken for analysis of protein,fat,solids-not-fat,lactose,and somatic cell count.Blood samples were collected on days-6,-3,0,3,6 relative to calving,and then monthly throughout lactation,and analyzed for P and Ca concentrations.Spot samples of feces and urine were collected for 3 consecutive d during weeks 12,24,and 36,and P concentrations were analyzed.Reproduction and health data were recorded.Results:Dietary P did not affect dry matter intake or milk yield(P> 0.10).Milk fat content was slightly higher in cows fed 0.37%P than in cows fed 0.47%P(P = 0.05).Serum concentrations of P and Ca did not reflect dietary P content(P> 0.10).Fecal and urinary P both declined linearly(P< 0.05) as dietary P decreased from 0.57 to0.37%.Fecal P content was 25%less when dietary P was 0.37%compared to 0.57%.Health events and reproductive performance were not associated with dietary P content(P> 0.05).Conclusions:Lowering dietary P from 0.57 to 0.37%did not negatively affect milk production,but did significantly reduce P excretion into environment. | Chong Wang Zhen Liu Diming Wang Jianxin Liu Hongyun Liu Zhiguo Wu | 2014 | Journal of Animal Science and Biotechnology2014，5，4： | 5 |
| 3 | Nitrogen partitioning and microbial protein synthesis in lactating dairy cows with different phenotypic residual feed intake显示文摘Background: Residual feed intake(RFI) is an inheritable measure of feed efficiency that is independent on level of production. However, physiological and metabolic mechanisms underlying divergent RFI are not fully elucidated.This study was conducted to investigate dietary nitrogen(N) partitioning and microbial protein synthesis in lactating dairy cows divergent in phenotypic RFI.Results: Thirty Holstein dairy cows(milk yield = 35.3 ± 4.71 kg/d;milk protein yield = 1.18 ± 0.13 kg/d;mean ± standard deviation) were selected for the experiment to derive RFI. After the RFI measurement period of 50 d, the 10 lowest RFI cows and 8 highest RFI cows were selected. The low RFI cows had lower dry matter intake(DMI, P < 0.05) than the high RFI cows, but they produced similar energy-corrected milk. The ratios of milk to DMI(1.41 vs. 1.24, P < 0.01) and energy-corrected milk to DMI(1.48 vs. 1.36, P < 0.01) were greater in low RFI cows than those in the high RFI cows. The low RFI cows had lower milk urea nitrogen than that in the high RFI cows(P = 0.05). Apparent digestibility of nutrients did not differ between two groups(P > 0.10). Compared with high RFI animals, the low RFI cows had a lower retention of N(5.72 vs. 51.4 g/d, P < 0.05) and a higher partition of feed N to milk N(29.7% vs. 26.5%, P < 0.05).Conclusions: The results suggest that differences in N partition, synthesis of microbial protein, and utilization of metabolizable protein could be part of the mechanisms associated with variance in the RFI. | Yunyi Xie Zezhong Wu Diming Wang Jianxin Liu | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 4 | AMPK-mTOR pathway is involved in glucose-modulated amino acid sensing and utilization in the mammary glands of lactating goats显示文摘Background:The local supply of energy-yielding nutrients such as glucose seems to affect the synthesis of milk components in the mammary gland(MG).Thus,our study was conducted to investigate the effects of locally available MG glucose supply(LMGS)on amino acid(AA)sensing and utilization in the MG of lactating dairy goats.Six dosages of glucose(0,20,40,60,80,and 100 g/d)were infused into the MG through the external pudendal artery to investigate the dose-dependent changes in mammary AA uptake and utilization(Exp.1)and the changes in mRNA and protein expression of the AMPK-mTOR pathway(Expt.2).Results:In Exp.1,total milk AA concentration was highest when goats were infused with 60 g/d glucose,but lower when goats were infused with 0 and 100 g/d glucose.Increasing LMGS quadratically changed the percentages ofαS2-casein andα-lactalbumin in milk protein,which increased with infusions from 0 to 60 g/d glucose and then decreased with infusions between 60 and 100 g/d glucose.The LMGS changed the AA availability and intramammary gland AA utilization,as reflected by the mammary AA flux indexes.In Exp.2,the mRNA expression of LALBA in the MG increased quadratically with increasing LMGS,with the highest expression at dose of 60 g/d glucose.A high glucose dosage(100 g/d)activated the general control nonderepressible 2 kinase,an intracellular sensor of AA status,resulting in a reduced total milk AA concentration.Conclusions:Our new findings suggest that the lactating MG in dairy goats may be affected by LMGS through regulation of the AA sensory pathway,AA utilization and protein synthesis,all being driven by the AMPK-mTOR pathway. | Jie Cai Diming Wang Feng-Qi Zhao Shulin Liang Jianxin Liu | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 3 |
| 5 | Constraints on the utilization of cereal straw in lactating dairy cows:A review from the perspective of systems biology显示文摘Cereal straw,a human inedible crop byproduct,can be used as a roughage source in ruminants.However,the nutrition density and palatability are very low,limiting its efficient utilization in animal production.This review aims to systematically provide an overview of the limitations of cereal straws,which is crucial for developing new strategies to enhance the efficient use of cereal straws by lactating dairy cows.Evolutionary molecular biology makes it possible to comprehensively understand the limitations of using cereal straw as a roughage source in dairy cows by different techniques,e.g.,multi-omics.Main constraints for utilization of cereal straw and stover in lactating dairy cows include low contents of easily fermented carbohydrates(pectin)and essential amino acids(Met,Phe,and branched-chain amino acids),high content of lignin and silica,and low nutrient digestibility.These cause insufficient supply of the precursors for milk synthesis and result in increased loss of nutrients in feces and urine.Several molecular mechanisms are revealed by multi-omics techniques,including changed amino acid and glucose metabolism,altered rumen microbial composition and function,and differential expression of miRNAs,mRNA,and protein in multi-organs that are associated with milk synthesis.These can be targets of approaches to improve the utilization of cereal straw by dairy cows.In addition,much attention should be given to the efficient countermeasures,including pretreatments by fibrolytic enzymes or steam explosion,dietary formulations such as supplement of pectin,methionine,and branched-chain amino acids,and feeding with other functional feedstuffs,which may improve the feeding and economic value of cereal straw for lactating dairy cows.The newly revealed functional genes(such as BAG3 in the rumen,PC in the liver,CSN1S2 in the mammary gland)and biomarkers(hippuric acid)as well as the integrative signaling and metabolic pathways(phenylalanine metabolism)related to the shortages of cereal straws could be used as nutritional or genetic regulatory targets to improve dairy cow production. | Bing Wang Huizeng Sun Diming Wang Hongyun Liu Jianxin Liu | 2022 | Animal Nutrition2022，，2： | 3 |
| 6 | Preliminary Experimental Results of ac Operation in the CT-6B Tokamak显示文摘The preliminary experimental results of ac plasma discharges in the CT-6B tokamak have been achieved by an active plasma current feedback system and the keys to a smooth transition through current zero and some phenomena related to the current reversal phase are also presented. | YANG Xuanzong ZHENG Shaobai JIANG Diming HAN Gonghe FENG Chunhua QI Xiazhi WANG Wenshu LI Wenlai LI Zanliang DONG Lifang | 1995 | Chinese Physics Letters1995，12，6： | 2 |
| 7 | Spectroscopic studies of structures of butyl and bromobutyl 显示文摘 | Cheng Diming Cheng Jin Wang Hecu | 1990 | Rubb Chem Technol1990，63，2： | 1 |
| 8 | An overview of APSIM, a model designed for farming systems simulation 显示文摘 | Keating B A Carberry P S Hammer G L Probert M E Robertson M J Holzworth D Huth N I Hargreaves J N G Meinke H Hochman Z McLean G Verburg K Snow V Dimes J P Silburn M Wang E Brown S Bristow K L Asseng S Chapman S McCown R L Freebairn D M Smith C J | 2003 | European Journal of Agronomy2003，18，314： | 1 |
| 9 | An overview of APSIM, a model designed for farming systems simulation显示文摘 | B.A Keating P.S Carberry G.L Hammer M.E Probert M.J Robertson D Holzworth N.I Huth J.N.G Hargreaves H Meinke Z Hochman G McLean K Verburg V Snow J.P Dimes M Silburn E Wang S Brown K.L Bristow S Asseng S Chapman R.L McCown D.M Freebairn C.J Smith | 2002 | European Journal of Agronomy2002，，3： | 1 |
| 10 | Helicon discharge using Nagoya type Ⅲ antenna显示文摘 | Fang Tongzhen Wang Long Jiang Diming | 2001 | Chinese Physics Letter2001，8，18： | 1 |
| 11 | A biosensing system employing nanowell microelectrode arrays to record the intracellular potential of a single cardiomyocyte显示文摘Electrophysiological recording is a widely used method to investigate cardiovascular pathology,pharmacology and developmental biology.Microelectrode arrays record the electrical potential of cells in a minimally invasive and highthroughput way.However,commonly used microelectrode arays primarily employ planar microelectrodes and cannot work in applications that require a recording of the intracellular action potential of a single cell.In this study,we proposed a novel measuring method that is able to record the intracellular action potential of a single cardiomyocyte by using a nanowell patterned microelectrode array(NWMEA).The NWMEA consists of five nanoscale wells at the center of each circular planar microelectrode.Biphasic pulse electroporation was applied to the NWMEA to penetrate the cardiomyocyte membrane,and the intracellular action potential was continuously recorded.The intracellular potential recording of cardiomyocytes by the NWMEA measured a potential signal with a higher quality(213.76±25.85%),reduced noise root-mean-square(~33%),and higher signal-to-noise ratio(254.36±12.61%)when compared to those of the extracellular recording.Compared to previously reported nanopillar microelectrodes,the NWMEA could ensure single cell electroporation and acquire high-quality action potential of cardiomyocytes with reduced fabrication processes.This NWMEA-based biosensing system is a promising tool to record the intracellular action potential of a single cell to broaden the usage of microelectrode arrays in electrophysiological investigation. | Yuting Xiang Haitao Liu Wenjian Yang Zhongyuan xu Yue Wu Zhajian Tang Zhjing Zhu Zhiyong Zeng Depeng Wang Tianxing Wang Ning Hu Diming Zhang | 2022 | Microsystems & Nanoengineering2022，8，3： | 0 |
| 12 | A biosensing system using a multiparameter nonlinear dynamic analysis of cardiomyocyte beating for drug-induced arrhythmia recognition显示文摘Cardiovascular disease is the number one cause of death in humans.Therefore,cardiotoxicity is one of the most important adverse effects assessed by arrhythmia recognition in drug development.Recently,cell-based techniques developed for arhythmia recognition primarily employ linear methods such as time-domain analysis that detect and compare individual waveforms and thus fall short in some applications that require automated and efficient arrhythmia recognition from large datasets.We carried out the frst report to develop a biosensing system that integrated impedance measurement and multiparameter nonlinear dynamic algorithm(MNDA)analysis for druginduced arrhythmia recognition and classification.The biosensing system cultured cardiomyocytes as physiologically relevant models,used interdigitated electrodes to detect the mechanical beating of the cardiomyocytes,and employed MNDA analysis to recognize drug-induced arrhythmia from the cardiomyocyte beating recording.The best performing MNDA parameter,approximate entropy,enabled the system to recognize the appearance of sertindoleand norepinephrine-induced arrhythmia in the recording.The MNDA reconstruction in phase space enabled the system to classify the different arrhythmias and quantify the severity of arrhythmia.This new biosensing system utilizing MNDA provides a promising and alternative method for drug-induced arrhythmia recognition and classification in cardiological and pharmaceutical applications. | Hao Wang Yue Wu Quchao Zou Wenjian Yang Zhongyuan Xu Hao Dong Zhjing Zhu Depeng Wang Tanxing Wang Ning Hu Diming Zhang | 2022 | Microsystems & Nanoengineering2022，8，3： | 0 |
| 13 | Supplementing N-carbamoylglutamate in late gestation increases newborn calf weight by enhanced placental expression of mTOR and angiogenesis factor genes in dairy cows显示文摘The objective of this study was to investigate whether supplementation with N-carbamoylglutamate(NCG)to cows during late gestation alters uteroplacental tissue nutrient transporters,calf metabolism and newborn weight.Thirty multiparous Chinese Holstein cows were used in a randomized complete block design experiment.During the last 28 d of pregnancy,cows were fed a diet without(CON)or with NCG(20 g/d per cow).The body weight of calves was weighed immediately after birth.Placentome samples were collected at parturition and used to assess mRNA expression of genes involved in transport of arginine,glucose,fatty acid and angiogenesis factors,as well as the mammalian target of rapamycin(mTOR)pathway.Blood samples of calves before colostrum consumption were also collected for the detection of plasma parameters,amino acids(AA)and metabolomics analysis.The newborn weight(P=0.02)and plasma Arg concentration of NCG-calves was significantly higher(P=0.05)than that of CON-calves,and the plasma concentrations of urea nitrogen tended to be lower(P=0.10)in the NCG group.The mRNA abundance of genes involved in glucose transport(solute carrier family 2 member 3[SLC2 A3],P<0.01),angiogenesis(nitric oxide synthase 3[NOS3],P=0.02),and mTOR pathway(serine/threonine-protein kinase 1[ACT1],P=0.10;euka ryotic translation initiation factor 4 B pseudogene 1[EIF4 BP1],P=0.08;EIF4 EBP2,P=0.04;and E74-like factor 2[ELF2],P=0.03)was upregulated in the placentome of NCG-supplemented cows.In addition,17 metabolites were significantly different in the placentome of NCG-supplemented cows compared to non-supplemented cows,and these metabolites are mainly involved in arginine and proline metabolism,alanine,aspartate and glutamate metabolism,and citrate cycle.In summary,the increased body weight of newborn calves from the NCG supplemented dairy cows maybe attributed to the increased angiogenesis and uteroplacental nutrient transport and to the activated mTOR signal pathway,which may result in the increased nutrient supply to the fetus,and improved AA metabolism and urea cycle of the fetus. | Fengfei Gu Luyi Jiang Linyu Xie Diming Wang Fengqi Zhao Jianxin Liu | 2021 | Animal Nutrition2021，，4： | 0 |
| 14 | Nano-sized zinc addition enhanced mammary zinc translocation without altering health status of dairy cows显示文摘This study aimed to evaluate role of nano-sized zinc(Zn)on lactation performance,health status,and mammary permeability of lactating dairy cows.Thirty multiparous dairy cows with similar days in milk(158±43.2)and body weight(694±60.5 kg)were chosen based on parity and milk production and were randomly assigned to 3 treatment groups:basal diet(control,69.6 mg/kg of Zn adequate in Zn requirement),basal diet additional Zn-methionine(Zn-Met,providing 40 mg/kg of Zn),and basal diet additional nano-sized Zn oxide(nZnO,providing 40 mg/kg of Zn).The study lasted for 10 wk,with the first 2 wk as adaptation.Feed intake,milk yield and the related variables,and plasma variables were determined every other week.Blood hematological profiles were determined in the 8 th week of the study.We found that feed intake,milk yield,and milk composition were similar across the 3 groups.The nZnO-and Zn-Met-fed cows had greater milk Zn concentrations in the milk(3.89 mg/L(Zn-Met)and3.93 mg/L(nZnO))and plasma(1.25 mg/L(Zn-Met)and 1.29 mg/L(nZnO))than the control cows(3.79 mg/L in milk and 1.21 mg/L in plasma).The nZnO-fed cows had higher Zn concentrations in plasma but not in milk compared to Zn-Met-fed cows.The Zn appearance in milk was greater in nZnO-fed(area under curve during the first 4 h post-feeding for milk Zn:16.1 mg/L)and Zn-Met-fed cows(15.7 mg/L)than in control cows(15.0 mg/L).During the first 4 h post-feeding,milk to blood Zn ratio was greater in nZnO-fed animals but lower in Zn-Met-fed cows compared with control cows.Oxidative stress-related variables in plasma,blood hematological profiles,and mammary permeability related variables were not different across treatments.In summary,lactation performance,Zn concentrations in milk and plasma,hematological profiles,mammary permeability were similar in cows fed nZnO and Zn-Met.We therefore suggested that nZnO feeding can improve Zn bioavailability without impairing lactation performance,health status,and mammary gland permeability in dairy cows. | Jie Cai Chao Miao Yi Chen Yunyi Xie Jianxin Liu Diming Wang | 2021 | Animal Nutrition2021，，4： | 0 |
| 15 | Heat stress affects dairy cow health status through blood oxygen availability显示文摘Background Rises in global warming and extreme weather occurrence make the risk of heat stress(HS)induced by high ambient temperatures more likely in high-yielding dairy cows,resulting in low milk quality and yield.In ani-mals,oxygen is involved in many physiological and metabolic processes,but the effects of HS on oxygen metabolism remain unclear.Thus,the current study aimed to investigate how oxygen metabolism plays a role in health status of dairy cows by measuring the milk yield,milk composition,and blood biochemical variables of cows under different levels of HS:none(No-HS),mild(Mild-HS),and moderate HS(Mod-HS).Results The HS significantly increased rectal temperature(Ptreat<0.01)and respiration rate(Ptreat<0.01).Under Mod-HS,greater Na+(P<0.05)and lower total CO_(2),and pH(P<0.05)were observed relative to those under No-HS and Mild-HS.Oxygen concentrations in both coccygeal artery and mammary vein(Ptreat<0.01)were lower under Mod-HS than under No-HS.Coccygeal vein concentrations of heat shock protein 90(HSP90)(P<0.05)increased during Mod-HS compared with those in cows under No-HS.Malondialdehyde increased during Mod-HS,and glu-tathione peroxidase(P<0.01)increased during Mild-HS.Coccygeal vein concentrations of vascular endothelial growth factor(P<0.01),heme oxygenase-1(P<0.01),and hypoxia-inducible factor 1α(P<0.01)were greater in cows under Mod-HS than those under No-HS.Red blood cell count(P<0.01)and hemoglobin concentration(P<0.01)were lower in the coccygeal vein of dairy cows under Mild-and Mod-HS than those of cows under No-HS.Conclusions Exposure to HS negatively impacts the health status and lactation performance of dairy cows by limit-ing oxygen metabolism and transportation.However,the specific mechanism by which HS affects mammary function in cows remains unclear and requires further exploration. | Jia Zeng Jie Cai Diming Wang Hongyun Liu Huizeng Sun Jianxin Liu | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
| 16 | Depth determination of the Moho interface beneath the Tibetan plateau and other areas of China显示文摘We apply the adaptive moving window method of Sun et al. to the most recent catalog data and the data recorded by portable stations to construct the velocity structure of the crust and upper mantle, and to determine the depth of the Moho interface beneath the Tibetan plateau and other areas of China. We first select 2 600 locations in the study region with 1 intervals, then at each location invert for a five-layer 1-D P-wave velocity model from the surface down to the uppermost mantle by performing a Monte Carlo random search. The Moho depth at each location is then determined, and the Moho interface beneath the study region is obtained through proper interpolation with certain smoothing. Compared to depths obtained by previous studies, our results show more accurate Moho depths in the Tibetan plateau, Tianshan region and other areas of the study region. | Youshun Sun Diming Yu M Nafi Toksz F Dale Morgan Xiyu Wang Jinrong Su Jun Liu | 2012 | Earthquake Science2012，25，5： | 0 |
| 17 | Selective catalytic reduction failure of low NH_(3)-NO_(x) ratio显示文摘An insufficient amount of NH_(3) (ammonia)will reduce the conversion efficiency of NO_(x),which may lead to excess NO_(x) emissions,resulting in NH3SCR failure.In this article,SCR failure caused by a low NH_(3)NO_(x) ratio is studied systematically by experiments.The main reasons for a low NH_(3)NO_(x) ratio in SCR include insufficient urea injection,hydrothermal aging of catalysts and urea crystallization.It was found from an insufficient urea injection experiment that with the increase of NH_(3)NO_(x) ratio,the NO_(x) conversion efficiency of the SCR system increased,but the ammonia leakage also increased.The main influencing factors of NO_(x) conversion efficiency are different under different NH3NOx ratios.A flow reactor system was used in the catalyst hydrothermal aging experiment to investigate the effect of hydrothermal aging on catalyst activity.After a 24 h hydrothermal aging experiment at 800℃,the NO_(x) conversion efficiency of the copperbased zeolite catalysts decreased significantly at the boundary of medium and low temperature regions.And the NO_(2)-NO_(x) ratio in the mixture had a significant effect on the catalytic performance.Thermogravimetry coupled to Fourier transform infrared spectroscopy(TGFTIR)was used to analyze the composition of urea deposits in a urea deposits analysis experiment.It was found that the main components of urea deposits were urea and isocyanic acid(HNCO).Preventing HNCO polymerization,especially the formation of CYA,can decrease the formation of urea deposits. | Piqiang Tan Xiaoyu Li Shiyan Wang Zhiyuan Hu Diming Lou | 2021 | Chinese Journal of Chemical Engineering2021，34，4： | 0 |
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