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| 1 | Characterization of HULC, a Novel Gene With Striking Up-Regulation in Hepatocellular Carcinoma, as Noncoding RNA显示文摘 | Katrin Panzitt Marisa M.O. Tschernatsch Christian Guelly Tarek Moustafa Martin Stradner Heimo M. Strohmaier Charles R. Buck Helmut Denk Renée Schroeder Michael Trauner Kurt Zatloukal | 2007 | Gastroenterology2007，，1： | 4 |
| 2 | Histological grading and staging of chronic hepatitis显示文摘 | Kamal Ishak Amelia Baptista Leonardo Bianchi Francesco Callea Jan De Groote Fred Gudat Helmut Denk Valeer Desmet Gerhard Korb Roderick N.M. MacSween M.James Phillips Bernard G. Portmann Hemming Poulsen Peter J. Scheuer Martin Schmid Heribert Thaler | 1995 | Journal of Hepatology1995，，6： | 4 |
| 3 | Histological grading and staging of chronic hepatitis显示文摘 | Kamal Ishak Amelia Baptista Leonardo Bianchi Francesco Callea Jan De Groote Fred Gudat Helmut Denk Valeer Desmet Gerhard Korb Roderick N.M. MacSween M.James Phillips Bernard G. Portmann Hemming Poulsen Peter J. Scheuer Martin Schmid Heribert Thaler | 1995 | Journal of Hepatology1995，，6： | 4 |
| 4 | Role of interleukin-1 and its antagonism of hepatic stellate cell proliferation and liver fibrosis in the Abcb4^(-/-) mouse model显示文摘AIM: To study the interleukin-1(IL-1) pathway as a therapeutic target for liver fibrosis in vitro and in vivo using the ATP-binding cassette transporter b4^(-/-)(Abcb4^(-/-)) mouse model.METHODS: Female and male Abcb4^(-/-) mice from 6 to 13 mo of age were analysed for the degree of cholestasis(liver serum tests), extent of liver fibrosis(hydroxyproline content and Sirius red staining) and tissue-specific activation of signalling pathways such as the IL-1 pathway [quantitative polymerase chain reaction(q PCR)]. For in vivo experiments, murine hepatic stellate cells(HSCs) were isolated via pronasecollagenase perfusion followed by density gradient centrifugation using female mice. Murine HSCs were stimulated with up to 1 ng/m L IL-1β with or without 2.5 μg/m L Anakinra, an IL-1 receptor antagonist, respectively. The proliferation of murine HSCs was assessed via the Brd U assay. The toxicity of Anakinra was evaluated via the fluorescein diacetate hydrolysis(FDH) assay. In vivo 8-wk-old Abcb4^(-/-) mice with an already fully established hepatic phenotype were treated with Anakinra(1 mg/kg body-weight daily intraperitoneally) or vehicle and liver injury and liver fibrosis were evaluated via serum tests, q PCR, hydroxyproline content and Sirius red staining. RESULTS: Liver fibrosis was less pronounced in males than in female Abcb4^(-/-) animals as defined by a lower hydroxyproline content(274 ± 64 μg/g vs 436 ± 80 μg/g liver, respectively; n = 13-15; P < 0.001; MannWhitney U-test) and lower m RNA expression of the profibrogenic tissue inhibitor of metalloproteinase-1(TIMP)(1 ± 0.41 vs 0.66 ± 0.33 fold, respectively; n = 13-15; P < 0.05; Mann-Whitney U-test). Reduced liver fibrosis was associated with significantly lower levels of F4/80 m RNA expression(1 ± 0.28 vs 0.71 ± 0.41 fold, respectively; n = 12-15; P < 0.05; Mann-Whitney U-test) and significantly lower IL-1β m RNA expression levels(1 ± 0.38 vs 0.44 ± 0.26 fold, respectively; n = 13-15; P < 0.001; Mann-Whitney U-test). No gender differences in the serum liver parameters [bilirubin; alanine aminotransferase(ALT); aspartate aminotransferase and alkaline phosphatase(AP)] were found. In vitro, the administration of IL-1β resulted in a significant increase in HSC proliferation [0.94 ± 0.72 arbitrary units(A.U.) in untreated controls, 1.12 ± 0.80 A.U. at an IL-1β concentration of 0.1 ng/m L and 1.18 ± 0.73 A.U. at an IL-1β concentration of 1 ng/m L in samples from n = 6 donor animals; P < 0.001; analyses of variance(ANOVA)]. Proliferation was reduced significantly by the addition of 2.5 μg/m L Anakinra(0.81 ± 0.60 A.U. in untreated controls, 0.92 ± 0.68 A.U. at an IL-1β concentration of 0.1 ng/m L, and 0.91 ± 0.69 A.U. at an IL-1β concentration of 1 ng/m L; in samples from n = 6 donor animals; P < 0.001; ANOVA) suggesting an anti-proliferative effect of this clinically approved IL-1 receptor antagonist. The FDH assay showed this dose to be non-toxic in HSCs. In vivo, Anakinra had no effect on the hepatic hydroxyprolinecontent, liver serum tests(ALT and AP) and profibrotic(collagen 1α1, collagen 1α2, transforming growth factor-β, and TIMP-1) and anti-fibrotic [matrix metalloproteinase 2(MMP2), MMP9 and MMP13 ] gene expression after 4 wk of treatment. Furthermore, the hepatic IL-1β and F4/80 m RNA expression levels were unaffected by Anakinra treatment.CONCLUSION: IL-1β expression is associated with the degree of liver fibrosis in Abcb4^(-/-) mice and promotes HSC proliferation. IL-1 antagonism shows antifibrotic effects in vitro but not in Abcb4^(-/-) mice. | Florian P Reiter Ralf Wimmer Lena Wottke Renate Artmann Jutta M Nagel Manuel O Carranza Doris Mayr Christian Rust Peter Fickert Michael Trauner Alexander L Gerbes Simon Hohenester Gerald U Denk | 2016 | World Journal of Hepatology2016，8，8： | 3 |
| 5 | Topology-Optimized 4D Printing of a Soft Actuator显示文摘Soft robots and actuators are emerging devices providing more capabilities in the field of robotics.More flexibility and compliance attributing to soft functional materials used in the fabrication of these devices make them ideal for delivering delicate tasks in fragile environments,such as food and biomedical sectors.Yet,the intuitive nonlinearity of soft functional materials and their anisotropic actuation in compliant mechanisms constitute an existent challenge in improving their performance.Topology optimization(TO)along with four-dimensional(4D)printing is a powerful digital tool that can be used to obtain optimal internal architectures for the efficient performance of porous soft actuators.This paper employs TO analysis for achieving high bending deflection of a 3D printed polyelectrolyte actuator,which shows bending deformations in response to electrical stimuli in an electrolyte solution.The performance of the actuator is studied in terms of maximum bending and actuation rate compared with a solid,uniformly 3D printed and topology-optimized actuator.The experimental results proved the effectiveness of TO on achieving higher bending deformation and actuation rate against a uniformly 3D printed actuator. | Ali Zolfagharian Martin Denk Mahdi Bodaghi Abbas Z.Kouzani Akif Kaynak | 2020 | Acta Mechanica Solida Sinica2020，33，3： | 3 |
| 6 | Feasibility of radical sigmoid colectomy performed as natural orifice translumenal endoscopic surgery (NOTES) using transanal endoscopic microsurgery显示文摘 | M. H. Whiteford P. M. Denk L. L. Swanstr?m | 2007 | Surgical Endoscopy2007，，10： | 3 |
| 7 | Results of a Summer Academy to Increase Minority Student Access to Allied Health and Other Health Professions显示文摘 | Thomson W A Denk J P Miller L M | 1991 | J Allied Health1991，21，1： | 1 |
| 8 | A community-based, randomized trial of ezetimibe added to statin therapy to attain NCEP ATP IlI goals for LDL cholesterol in hypercholesterolemic patients: the ezetimibe add-on to statin for effectiveness (EASE) trial 显示文摘 | Pearson TA Denke MA McBride PE | 2005 | Mayo Clin Proc2005，80，5： | 1 |
| 9 | p53 mutations are rare events in recurrent cervical cancer显示文摘 | Denk C Butz K Schneider A | | 0，，： | 1 |
| 10 | Histopathological diagnosis of non-alcoholic and alcoholic fatty liver disease显示文摘 | Tannapfel A Denk H Dienes HP | 2011 | Virchows Arch2011，458，5： | 1 |
| 11 | A community-based, randomized trial of ezetimibe added to statin therapy to attain NCEP ATP Ⅲ goals for LDL cholesterol in hypercholesterolemic patients: the ezetimibe add-on to statin for effectiveness (EASE) trial显示文摘 | Pearson TA Denke MA McBride PE | 2005 | Mayo Clin Proc2005，80，5： | 1 |
| 12 | Effect of growth factors on the activation of human Tenon's capsule fibroblasts显示文摘 | Denk PO Hoppe J Hoppe V | 2003 | Curr Eye Res2003，27，1： | 1 |
| 13 | Two-photon Laser Scanning Fluorescence Microscopy 显示文摘 | DENK W STRICKLER J H | 1990 | Science1990，248，1： | 1 |
| 14 | p53 mutations are rare events in recurrent cervical cancer 显示文摘 | Denk C Butz K Schneider A | 2001 | J Mol Med (Berl)2001，79，56： | 1 |
| 15 | 显示文摘 | Denk W Strickhr J H Webb W W | 1990 | Science1990，248，： | 1 |
| 16 | Residual stress fields in surface- treated silicon carbide for space industry-comparison of biaxial and triaxial analysis using differebt X-ray methods显示文摘 | Genzel CH KLAUS M DENKS I | 2005 | Materials Science and Engineering2005，390，： | 1 |
| 17 | How many lymph nodes are necessary to stage early and advanced adenocarcinoma of the sigmoid colon and upper rectum 显示文摘 | Leibl S Tsybrovskyy O Denk H | 2003 | Virchows Arch2003，443，2： | 1 |
| 18 | A community-based, randomized trial of ezetimibe added to statin therapy to attain NCEP ATP Ⅲ goals for LDL cholesterol in hypercholesterolemic patients: the ezetimibe add-on to statin for effectiveness (EASE) trial显示文摘 | Pearson TA Denke MA McBride PE | 2005 | Mayo Clin Proc2005，80，5： | 1 |
| 19 | Induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral E6 oncoprotein 显示文摘 | Butz K Denk C Ullmann A Scheffner M | 2000 | Proc Natl Acad Sci USA2000，97，12： | 1 |
| 20 | Prognostic factors for swallowing rehabilitation following head and neck cancer surgery显示文摘 | Denk D M Swoboda H Schima W | 1997 | Acta Otolaryngol1997，117，5： | 1 |
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