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| 1 | Overexpression of the maize GRF10, an endogenous truncated growth-regulating factor protein, leads to reduction in leaf size and plant height显示文摘It has long been thought that growth‐regulating factors(GRFs) gene family members act as transcriptional activators to play important roles in multiple plant developmental processes. However, the recent characterization of Arabidopsis GRF7 showed that it functions as a transcriptional repressor of osmotic stress‐responsive genes. This highlights the complex and diverse mechanisms by which different GRF members use to take action. In this study, the maize(Zea mays L.) GRF10 was functionally characterized to improve this concept. The deduced ZmGRF10 protein retains the N‐terminal QLQ and WRC domains, the characteristic regions as protein‐interacting and DNA‐binding domains, respectively. However,it lacks nearly the entire C‐terminal domain, the regions executing transactivation activity. Consistently, ZmGRF10 protein maintains the ability to interact with GRF‐interacting factors(GIFs) proteins, but lacks transactivation activity.Overexpression of ZmGRF10 in maize led to a reduction in leaf size and plant height through decreasing cell proliferation,whereas the yield‐related traits were not affected. Transcriptome analysis revealed that multiple biological pathways were affected by ZmGRF10 overexpression, including a few transcriptional regulatory genes, which have been demonstrated to have important roles in controlling plant growth and development. We propose that ZmGRF10 aids in fine‐tuning the homeostasis of the GRF‐GIF complex in the regulation of cell proliferation. | Lei Wu Dengfeng Zhang Ming Xue Jianjun Qian Yan He Shoucai Wang | 2014 | Journal of Integrative Plant Biology2014，56，11： | 31 |
| 2 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 3 | Geochemistry, geochronology and Hf isotope of granitoids in the Chinese Altai: Implications for Paleozoic tectonic evolution of the Central Asian Orogenic Belt显示文摘The Chinese Altai in northwestern Xinjiang has numerous outcrops of granitoids which provide critical information on accretionary orogenic processes and crustal growth of the Central Asian Orogenic Belt.Zircon U-Pb ages, Hf-isotopic compositions and whole-rock geochemistry of monzogranite and granodiorites in the Qinghe County are employed to elucidate Paleozoic tectonics of the Chinese Altai. Granodiorites have crystallization ages of 424.6 ± 3.1 Ma(MSWD = 0.23) and 404.0 ± 3.4 Ma(MSWD = 0.18);monzogranite was emplaced in the early Permian with a crystallization age of 293.7 ± 4.6 Ma(MSWD = 1.06). Both granodiorites and monzogranite are I-type granites with A/CNK ratios of 0.92 -0.97 and 1.03 -1.06, respectively. They also show similar geochemical features of high HREE and Y contents, low Sr contents and Sr/Y ratios, as well as enrichment of Cs, Rb, Th and U, and depletion of Nb, Ta, P and Ti.These geochemical features indicate that the monzogranite and granodiorites were formed in an arc setting related to subduction. The gneissic monzogranites display high SiO_2 and K_2 O contents, and belong to the high-K calc-alkaline series. In the chondrite normalized REE distribution pattern, the monzogranite samples exhibit enrichment of LREE with strong negative Eu anomalies(σE u =0.44 -0.53), zircon εHf(t) values from +7.24 to +12.63 and two-stage Hf model ages of 463 -740 Ma. This suggests that the monzogranite was generated from the mixing of pelitic and mantle material. The granodiorite samples are calc-alkaline granites with lower contents of Si O_2 and Na_2 O + K_2 O, higher contents of TiO_2, Fe_2O_3~t, MgO and CaO compared to the monzogranite samples. They also show enrichment of LREE and moderate negative Eu anomalies(σE u= 0.54 =0.81), as well as slightly higher differentiation of LREE than that of HREE. The425 Ma granodiorite has zircon εHf(t) values from -0.51 to +1.98 and two-stage Hf model ages of 1133 -1240 Ma, whereas the 404 Ma granodiorite displays those of +2.52 to +7.50 and 816 -1071 Ma.Geochemistry and zircon Hf isotopic compositions indicate that granodiorites were formed by partial melting of juvenile lower crust. Together with regional geology and previous data, the geochemical and geochronological data of the monzogranite and granodiorites from this study suggest long-lived subduction and accretion along the Altai Orogen during ca. 425 -294 Ma. | Dengfeng He Yunpeng Dong Xueyi Xu Junlu Chen Xiaoming Liu Wei Li Xiangmin Li | 2018 | Geoscience Frontiers2018，9，5： | 3 |
| 4 | Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing显示文摘Background:Most traditional wound dressings only partially meet the needs of wound healing because of their single function.Patients usually suffer from the increasing cost of treatment and pain resulting from the frequent changing of wound dressings.Herein,we have developed a mutifunctional cryogel to promote bacterial infected wound healing based on a biocompatible polysaccharide.Methods:The multifunctional cryogel is made up of a compositive scaffold of chitosan(CS),gelatin(Gel)and tannic acid(TA)and in situ formed silver nanoparticles(Ag NPs).A liver bleeding rat model was used to evaluate the dynamic hemostasis performance of the various cryogels.In order to evaluate the antibacterial properties of the prepared cryogels,gram-positive bacterium Staphylococcus aureus(S.aureus)and gram-negative bacterium Escherichia coli(E.coli)were cultured with the cryogels for 12 h.Meanwhile,S.aureus was introduced to cause bacterial infection in vivo.After treatment for 2 days,the exudates from wound sites were dipped for bacterial colony culture.Subsequently,the anti-inflammatory effect of the various cryogels was evaluated by western blotting and enzyme-linked immunosorbent assay.Finally,full-thickness skin defect models on the back of SD rats were established to assess the wound healing performances of the cryogels.Results:Due to its porous structure,the multifunctional cryogel showed fast liver hemostasis.The introduced Ag NPs endowed the cryogel with an antibacterial efficiency of>99.9%against both S.aureus and E.coli.Benefited from the polyphenol groups of TA,the cryogel could inhibit nuclear factor-κB nuclear translocation and down-regulate inflammatory cytokines for an anti-inflammatory effect.Meanwhile,excessive reactive oxygen species could also be scavenged effectively.Despite the presence of Ag NPs,the cryogel did not show cytotoxicity and hemolysis.Moreover,in vivo experiments demonstrated that the biocompatible cryogel displayed effective bacterial disinfection and accelerated wound healing.Conclusions:The multifunctional cryogel,with fast hemostasis,antibacterial and anti-inflammation properties and the ability to promote cell proliferation could be widely applied as a wound dressing for bacterial infected wound healing. | Na Xu Yucheng Yuan Liangping Ding Jiangfeng Li Jiezhi Jia Zheng Li Dengfeng He Yunlong Yu | 2022 | Burns & Trauma2022，10，1： | 1 |
| 5 | Miniaturization of mechanical actuators in skin-integrated electronics for haptic interfaces显示文摘Skin-integrated electronics,also known as electronic skin(e-skin);are rapidly developing and are gradually being adopted in biomedical fields as well as in our daily lives.E-skin capable of providing sensitive and high-resolution tactile sensations and haptic feedback to the human body would open a new e-skin paradigm for closed-loop human-machine interfaces.Here,we report a class of materials and mechanical designs for the miniaturization of mechanical actuators and strategies for their integration into thin,soft e-skin for haptic interfaces.The mechanical actuators exhibit small dimensions of 5 mm diameter and 1.45 mm thickness and work in an electromagnetically driven vibrotactile mode with resonance frequency overlapping the most sensitive frequency of human skin.Nine mini actuators can be integrated simultaneously in a small area of 2 cm×2 cm to form a 3×3 haptic feedback array,which is small and compact enough to mount on a thumb tip.Furthermore,the thin,soft haptic interface exhibits good mechanical properties that work properly during stretching,bending,and twisting and therefore can conformally fit onto various parts of the human body to afford programmable tactile enhancement and Braille recognition with an accuracy rate over 85%. | Dengfeng Li Jiahui He Zhen Song Kuanming Yao Mengge Wu Haoran Fu Yiming Liu Zhan Gao Jingkun Zhou Lei Wei Zhengyou Zhang Yuan Dai Zhaoqian Xie Xinge Yu | 2021 | Microsystems & Nanoengineering2021，7，5： | 1 |
| 6 | Surface charge-convertible quaternary ammonium salt-based micelles for in vivo infection therapy显示文摘Quaternary ammonium salts(QASs) are excellent candidates for treating stubborn bacterial infections caused by biofilms due to their high sterilization efficiency and potential inhibition of the development of drug resistance.However,the inherent toxicity of QASs,including cytotoxicity,protein absorption and hemolysis,severely limits their applications in vivo.Herein,a charge-co nvertible quaternary ammonium salt-based micelle(QAS-SL@CM) was constructed by co-assembly of two amphiphiles with opposite charges and shell cross-linking strategy.The toxicity of the QAS-SL@CM could be greatly reduced towa rds human cells contrast to the corresponding QASs.By response to the acidic environment at infection sites,the surface charge of QAS-SL@CM could be immediately changed to positive and then target to negatively charged bacteria.Furthermo re,β-thiopropionate bonds on QAS-SL@CM could also be disintegrated under acid environment to release QASs to kill bacteria.Importantly,the QAS-SL@CM showed significant therapeutic effect in mice subcutaneous abscesses models without interference with normal cells.Therefore,a surface adaptive micelle constructed by charge-convertible strategy has been developed to ove rcome the cytotoxicity of QAS s,and could intelligently respond to the microenvironment of infected wound for in vivo infection therapy,which shows promising application in clinic. | Dengfeng He Yifeng Tan Pengfei Li Yadong Luo Yuhong Zhu Yunlong Yu Jiali Chen Ning Ning Shiyong Zhang | 2021 | Chinese Chemical Letters2021，32，5： | 1 |
| 7 | Ultra-low lattice thermal conductivity and promising thermoelectric figure of merit in borophene via chlorination显示文摘Monolayer boron-based materials are of current interests due to its polymorphism.Herein,motivated by the recent experimental synthesis of semiconducting hydrogenatedαʹ-borophene and the regulation of the physical properties in layered materials by surface functionalization,we study the thermal and electronic properties ofαʹ-borophene with three different types of gas functional groups(H,F,and Cl)based on first-principles and Boltzmann transport theory.It is found thatαʹ-borophene can be well stabilized by fluorination and chlorination and maintain the semiconductor nature.More interestingly,when hydrogen is replaced with fluorine or chlorine,the lattice thermal conductivity changes from 24.3 to 5.2 or 0.73 W/(m·K)along armchair direction at 300 K,exhibiting a huge reduction by two orders of magnitude.The main reason is the decrease of both phonon group velocities and acoustic phonon relaxation time resulting from the strong phonon mode softening due to the weaken B-B bond strength and heavier atomic mass of fluorine and chlorine.Consequently,the chlorinatedαʹ-borophene exhibits a high thermoelectric figure of merit~2 at 300 K along armchair direction.Our study illustrates the importance of the modulation of transport properties by gas functional groups,which may promote the thermoelectric application of boron-based materials. | Jia He Yanxiao Hu Dengfeng Li Jie Chen | 2022 | Nano Research2022，15，4： | 1 |
| 8 | Inheritance of seeds per silique in Brassica napus L. using joint segregation analysis显示文摘 | Liwu Zhang Pingwu Liu Dengfeng Hong Anqun Huang Shipeng Li Qingbiao He Guangsheng Yang | 2009 | Field Crops Research2009，，1： | 1 |
| 9 | Azobenzene quaternary ammonium salt for photo-controlled and reusable disinfection without drug resistance显示文摘The abuse of antibiotics causes severe bacterial resistance, and the shortage of antibiotics has created a global public health crisis. This situation has prompted people to develop new antibacterial agents independent of traditional antibiotics. Here, we created a series of photosensitive azobenzene-quaternary ammonium salt smart antibacterial agents by connecting azobenzene with amines with different chain lengths to improve the antibacterial selectivity of quaternary ammonium salt(QAS) and prevent the accumulation of active QAS in the environment. After trans-cis isomerization, the solubility of the title compound(compound 4) increased and the antibacterial property enhanced. The experimental results suggested that the antibacterial effect of compound 4 was significantly enhanced after 365 nm light irradiation, and it had photosensitive intelligent antibacterial activity and could be reused. Notably, we did not obtain any mutants of Staphylococcus aureus or Escherichia coli resistant to compound 4. In general,compound 4 has the advantages of high yield, photo-controllable antibacterial properties, reusability, and does not induce bacterial resistance. This photosensitive antibacterial compound provides a new idea for the construction of intelligent disinfectants and is expected to be a candidate for disinfectants in public facilities and medical architecture. | Yuhong Zhu Pengfei Li Caikun Liu Mengqi Jia Yadong Luo Dengfeng He Chunyan Liao Shiyong Zhang | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
| 10 | Orbitally driven giant thermal conductance associated with abnormal strain dependence in hydrogenated graphene-like borophene显示文摘Heat energy in solids is carried by phonons and electrons.However,in most two-dimensional(2D)materials,the contribution from electrons to total thermal conduction is much lower than that for phonons.In this work,through first-principles calculations combined with non-equilibrium Green’s function theory,we studied electron and phonon thermal conductance in recently synthesized 2D hydrogen boride.The hexagonal boron network with bridging hydrogen atoms is suggested to exhibit comparable lattice thermal conductance(4.07 nWK−1 nm−2)as graphene(4.1 nWK−1 nm−2),and similar electron thermal conductance(3.6 nWK−1 nm−2),which is almost ten times that of graphene.As a result,total thermal conductance of 2D hydrogen boride is about two-fold of graphene,being the highest value in all known 2D materials.Moreover,tensile strain along the armchair direction leads to an increase in carrier density,significantly increasing electron thermal conductance.The increase in electron thermal conductance offsets the reduction in phonon thermal conductance,contributing to an abnormal increase in thermal conductance.We demonstrate that the high electron density governs extraordinarily high thermal conductance in 2D hydrogen boride,distinctive among 2D materials. | Jia He Dengfeng Li Yan Ying Chunbao Feng Junjie He Chengyong Zhong Hangbo Zhou Ping Zhou Gang Zhang | 2019 | npj Computational Materials2019，，1： | 0 |
| 11 | Functional genomics of Brassica napus:Progresses,challenges,and perspectives显示文摘Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil worldwide.Identification and understanding the gene functions in the B.napus genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B.napus.In this review,we present an overview of the progress made in the functional genomics of B.napus,including the availability of germplasm resources,omics databases and cloned functional genes.Based on the current progress,we also highlight the main challenges and perspectives in this field.The advances in the functional genomics of B.napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B.napus and will expedite the breeding of high quality,high resistance and high yield in B.napus varieties. | Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu Guangsheng Yang Tingdong Fu Liang Guo Hu Zhao | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
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