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| 1 | Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats显示文摘OBJECTIVE:To study the effect of Wuziyanzong treatment on the sperm quality and content of calcium ions(Ca 2+) in oligoasthenospermia rats.METHODS:A model of oligoasthenospermia was induced in 50 Sprague Dawley rats by treatment with tripterygium glycosides at 30 mg/kg per day for 8 weeks.They were divided randomly into a model group,a positive group(Huangjingzanyu capsule,3.01 g/kg),and low,medium and high dose Wuziyanzong treatment groups(2.30,4.60,9.20 g/kg crude drug respectively) with 10 in each group.Another 10 rats were used as a control group.The rats in the control and model groups were administered distilled water,while the rats in the remaining groups were administered Wuziyanzong for 30 d.The epididymides were removed,spermatozoa recovered and the sperm density and viability were measured.The spermatozoa were purified and the contents of Ca 2+ in the cytoplasm and mitochondria were detected by flow cytometry and atomic absorption spectrometry,respectively.RESULTS:After 8 weeks of treatment with tripterygium glycosides,the sperm density,sperm activity and the Ca 2 + content of spermatozoa in the model rats were all significantly decreased compared with the control group(all P<0.05).After 30 d treatment,the sperm density and activity improved and the Ca 2 + content of sperm were increased significantly in the medium and high dose Wuziyanzong treatment groups in comparison with the model group(all P<0.05).CONCLUSION:The Wuziyanzong treatment increased sperm density,improved sperm viability and enhanced the content of Ca 2+ in the sperm cytoplasm and mitochondria in this rat model of oligoasthenospermia. | Tongsheng Wang Jinling Huang Deling Wu Qing Li Xiangguo Liu Han Chen Lei Qiao Dongdong Li Jun Li | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 11 |
| 2 | ACTIVE FRONT STEERING DURING BRAKING PROCESS显示文摘An active front steering(AFS) intervention control during braking for vehicle stability is presented. Based on the investigation of AFS mechanism, a simplified model of steering system is established and integrated with vehicle model. Then the AFS control on vehicle handling dynamics during braking is designed. Due to the difficulties associated with the sideslip angle measurement of vehicle, a state observer is designed to provide real time estimation. Thereafter, the controller with the feedback of both sideslip and yaw angle is implemented. To evaluate the system control, the proposed AFS controlled vehicle has been tested in the Hardware-in-the-loop-simulation (HILS) system and compared with that of conventional vehicle. Results show that AFS can improve vehicle lateral stability effectively without reducing the braking performance. | CHEN Deling CHEN Li YIN Chengliang ZHANG Yong | 2008 | Chinese Journal of Mechanical Engineering2008，21，4： | 9 |
| 3 | Adaptive immune-genetic algorithm for global optimization to multivariable function显示文摘An adaptive immune- genetic algorithm (ALGA) is proposed to avoid premature convergence and guarantee the diversity of the population.Rapid immune response (secondary response),adaptive mutation and density operators in the AIGA are emphatically designed to improve the searching ability,greatly increase the converging speed,and decrease locating the local maxima due to the premature convergence.The simulation results obtained from the global optimization to four multivariable and multi-extreme functions show that AIGA converges rapidly,guarantees the diversity,stability and good searching ability. | Dai Yongshou Li Yuanyuan Wei Lei Wang Junling Zheng Deling | 2007 | Journal of Systems Engineering and Electronics2007，18，3： | 9 |
| 4 | Migration of Some Elements and Radionuclides across aGranite-Granite Contact Zone: A Natural Analoguefor Safe Disposal of High-Level Radwastes显示文摘Elements and natural radionuclides in the contact zone of two granites with different ages would migrate from one to the other because of the difference in their chemical contents and later water-rock interactions. This migration could serve as an analogue for the near-field process of radwastes in a high-level radwaste deep geological disposal repository.In the contact between the Indosinian granite (whole-rock Rb-Sr isochron age at 214@3 Ma) and Hercynian granite (zircon U-Pb isochron age at 296@31 Ma) located in Ziyuan County, Guangxi, the O and Pb isotope characteristics and the activity ratios of 234U/238U, 230Th/238U, 230Th/234U and 226Ra/230Th show that, based on the whole-rock chemical contents, both of the two granites have maintained a relatively open chemical system in their evolution processes. However, as there is no obvious open fault, the migration of major elements, trace elements and natural U-series nu-clides takes place within only 1-2 m in the contact zone, and water-rock | LUO Xingzhang MIN Maozhong ZHANG Guanghui LI Xianguo YANG Zhe ZHAI Liying Zhu Deling | 1999 | Acta Geologica Sinica(English Edition)1999，73，4： | 4 |
| 5 | The grafts modified by heparinization and catalytic nitric oxide generation used for vascular implantation in rats显示文摘Small-diameter(<6mm)vascular grafts are increasingly needed in peripheral vascular surgery but have few successes because of acute thrombosis,incomplete endothelialization and intimal hyperplasia after implantation.This study used electrospun poly(ε-caprolactone)as the matrix material.Heparin and selenium-containing catalyst-organoselenium modified polyethyleneimine were introduced through layer-by-layer assembly in order to build a vascular graft with in situ nitric oxide(NO)generation.The aim of this study was to explore the application of the graft with improved histocompatibility and biological function for vascular implantation in rats.After implantation in rats,compared to poly(ε-caprolactone),the modified grafts could promote the adhesion and proliferation of endothelial cells,and inhibit the adhesion of smooth muscle cells.The modified grafts remarkably promoted endothelialization,inhibited intimal hyperplasia and increased the ratio of alternatively activated macrophages(M2)to classical activated macrophages(M1).This work constructed a vascular graft with heparinization and catalytic NO generation for improving the vascularization,and accelerating the tissue regeneration by regulating the inflammatory response.The present study indicates that it is a promising method for regulating response and tissue regeneration of small diameter vascular grafts by a novel approach of combining heparinization and catalytic NO generation. | Jingchen Gao Li Jiang Qinge Liang Jie Shi Ding Hou Di Tang Siyuan Chen Deling Kong hufangWang | 2018 | Regenerative Biomaterials2018，5，2： | 4 |
| 6 | Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury显示文摘Endothelial cell therapy has been implicated to enhance tissue regeneration and vascularization in ischemic kidney. However, no published study has yet examined direct effects of endothelial cell treatment in kidney recovery. This study investigated the therapeutic efficacy of endothelial cells in a mouse model with acute kidney injury (AKI). Thus, human embryonic stem cells-derived endothelial cells (hESC-ECs) labeled with a reporter system encoding a double fusion reporter gene for firefly luciferase (Fluc) and green fluorescent protein (GFP) were characterized by Fluc imaging and immunofluoresence staining. Cultured hESC-ECs (1×106) were injected into ischemic kidney shortly after AKI. Survival of the transplanted hESC-ECs was monitored in vivo from day 1 to 14 after endothelial cell transplantation and potential impact of hESC-EC treatment on renal regeneration was assessed by histological analyses. We report that a substantial level of bioluminescence activity was detected 24 h after hESC-EC injection followed by a gradual decline from 1 to 14 d. Human ESC-ECs markedly accelerated kidney cell proliferation in response to ischaemia-induced damage, indicated by an elevated number of BrdU+ cells. Co-expression of Sca-1, a kidney stem cell proliferation marker, and BrdU further suggested that the observed stimulation in renal cell regeneration was, at least in part, due to increased proliferation of renal resident stem cells especially within the medullary cords and arteriole. Differentiation of hESC-ECs to smooth muscle cells was also observed at an early stage of kidney recovery. In summary, our results suggest that endothelial cell therapy facilitates kidney recovery by promoting vascularization, trans-differentiation and endogenous renal stem cell proliferation in AKI. | JIA XiaoHua L He LI Chen FENG GuoWei YAO XinPeng MAO LiNa KE TingYu CHE YongZhe XU Yong LI ZongJin KONG DeLing | 2013 | Chinese Science Bulletin2013，58，23： | 4 |
| 7 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 8 | Construction and evaluation of nitric oxide generating vascular graft material loaded with organoselenium catalyst via layer-by-layer self-assembly显示文摘A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide(NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione(GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts. | AN Jun CHEN SiYuan GAO JingChen ZHANG Xu WANG YuanYuan LI YanDong MIKHALOVSKY Sergey KONG DeLing WANG ShuFang | 2015 | Science China(Life Sciences)2015，58，8： | 3 |
| 9 | The “1-3-7” Approach to Malaria Surveillance and Response--Henan Province, China, 2012−2018显示文摘What is already known about this topic?The“1-3-7”approach to malaria surveillance and response was a key measure for malaria elimination in China and was first introduced into the World Health Organization(WHO)as an international guideline for malaria surveillance and response in 2018.What is added by this report?The“1-3-7”approach was well implemented in Henan Province from 2012−2018.Over this study period,a total of 1,294 malaria cases were detected and reported,and all cases were diagnosed and reported within 1 day with 99.23%(1,284/1,294)of cases were investigated within 3 days.In addition,93.7%(1,212/1,294)of foci were investigated and vector control was implemented within 7 days at all residual non-active foci to prevent further spread.What are the implications for public health practice?The“1-3-7”controlling pattern would be an effective and approachable method for implementation especially in malaria-eliminating countries and regions,but the interval from symptom onset to diagnosis cannot be ignored.Thus,the roles and responsibilities that all actors involved in the health sector must be specified too. | Qunqun Zhang Ying Liu Yabo Hu Yuling Zhao Chengyun Yang Dan Qian Ruimin Zhou Suhua Li Zhou Guan Deling Lu Hongwei Zhang Wanshen Guo | 2020 | China CDC weekly2020，2，17： | 3 |
| 10 | Diffuse Intrinsic Pontine Gliomas Exhibit Cell Biological and Molecular Signatures of Fetal Hindbrain-Derived Neural Progenitor Cells显示文摘Diffuse intrinsic pontine glioma(DIPG) is the main cause of brain tumor-related death among children.Until now, there is still a lack of effective therapy with prolonged overall survival for this disease. A typical strategy for preclinical cancer research is to find out the molecular differences between tumor tissue and para-tumor normal tissue, in order to identify potential therapeutic targets. Unfortunately, it is impossible to obtain normal tissue for DIPG because of the vital functions of the pons.Here we report the human fetal hindbrain-derived neural progenitor cells(pontine progenitor cells, PPCs) as normal control cells for DIPG. The PPCs not only harbored similar cell biological and molecular signatures as DIPG glioma stem cells, but also had the potential to be immortalized by the DIPG-specific mutation H3 K27 M in vitro. These findings provide researchers with a candidate normal control and a potential medicine carrier for preclinical research on DIPG. | Yu Sun Cheng Xu Changcun Pan Xin Chen Yibo Geng Yuliang Wu Peng Zhang Wenhao Wu Yu Wang Deling Li Zhen Wu Junting Zhang Qiaoran Xi Liwei Zhang | 2019 | Neuroscience Bulletin2019，35，2： | 2 |
| 11 | Hepatitis B virus X protein promotes hepatocellular carcinoma transformation through interleukin-6 activation of microRNA-21 expression显示文摘 | Chi Han Li Feiyue Xu Sheungching Chow Lu Feng Deling Yin Tzi Bun Ng Yangchao Chen | 2014 | European Journal of Cancer2014，，15： | 2 |
| 12 | Self-assembled PEG-b-PDPA-b-PGEM copolymer nanoparticles as protein antigen delivery vehicles to dendritic cells:preparation,characterization and cellular uptake显示文摘Antigen uptake by dendritic cells(DCs)is a key step for initiating antigen-specific T cell immunity.In the present study,novel synthetic polymeric nanoparticles were prepared as antigen delivery vehicles to improve the antigen uptake by DCs.Well-defined cationic and acid-responsive copolymers,monomethoxy poly(ethylene glycol)-block-poly(2-(diisopropyl amino)ethyl methacrylate)-block-poly(2-(guanidyl)ethyl methacrylate)(mPEG-b-PDPA-b-PGEM,PEDG)were synthesized by reversible addition-fragmentation chain transfer polymerization of 2-(diisopropylamino)ethyl methacrylate and N-(tert-butoxycarbonyl)amino ethyl methacrylate monomers,followed by deprotection of tert-butyl protective groups and guanidinylation of obtained primary amines.1H NMR,13C NMR and GPC results indicated the successful synthesis of well-defined PEDG copolymers.PEDG copolymers could self-assemble into nanoparticles in aqueous solution,which were of cationic surface charges and showed acid-triggered disassembly contributed by PGEM and PDPA moieties,respectively.Significantly,PEDG nanoparticles could effectively condense with negatively charged model antigen ovalbumin(OVA)to form OVA/PEDG nanoparticle formulations with no influence on its secondary and tertiary structures demonstrating by far-UV circular dichroism and UV-vis spectra.In vitro antigen cellular uptake by bone marrow DCs(BMDCs)indicated using PEDG nanoparticles as antigen delivery vehicles could significantly improve the antigen uptake efficiency of OVA compared with free OVA or the commercialized Alum adjuvant.Moreover,as the surface cationic charges of OVA/PEDG nanoparticle formulations reduced,the uptake efficiency decreased correspondingly.Collectively,our work suggests that guanidinylated,cationic and acid-responsive PEDG nanoparticles represent a new kind of promising antigen delivery vehicle to DCs and hold great potential to serve as immunoadjuvants in the development of vaccines. | Pan Li Junhui Zhou Pingsheng Huang Chuangnian Zhang Weiwei Wang Chen Lia Deling Kong | 2017 | Regenerative Biomaterials2017，4，1： | 2 |
| 13 | Prepara- tion and Thermo-oxidative Degradation of Poly (L-lactic acid)/Poly(L-lactic acid)-grafted SiO2 Nanocomposites 显示文摘 | Li Deling Liu Guangtian Wang Lihong | 2011 | Polymer Bulletin2011，67，8： | 1 |
| 14 | Stabilization of Chaotic Time Series by Proportional Pulse in the System Variable Based on Genetic Algorithm显示文摘The PPSV (Proportional Pulse in the System Variable) algorithm is a convenient method for the stabilization of the chaotic time series. It does not require any previous knowledge of the system. The PPSV method also has a shortcoming, that is, the determination off. is a procedure by trial and error, since it lacks of optimization. In order to overcome the blindness, GA (Genetic Algorithm), a search algorithm based on the mechanics of natural selection and natural genetics, is used to optimize the λi The new method is named as GAPPSV algorithm. The simulation results show that GAPPSV algorithm is very efficient because the control process is short and the steady-state error is small. | Qing Li Deling Zheng Jianlong Zhou(Information Engineering School, University of Science and Technology Beijing, Beijing 100083, China)(Handan iron and Steel Co., Handan 056015, China) | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，3： | 1 |
| 15 | Synthesis rheological behavior and mechanical properties of graft-type ACS resin 显示文摘 | Gao Jungang Sun Shuhui Li Deling | 2002 | Polym-Plast Technol Eng2002，1，5： | 1 |
| 16 | Targeting Phosphatidylcholine-Specific Phospholipase C for Atherogenesis Therapy显示文摘 | HaiYing Li Lu Zhang DeLing Yin Yun Zhang JunYing Miao | 2010 | Trends in Cardiovascular Medicine2010，，5： | 1 |
| 17 | Effect of Surface Lewis Acidity on Selective Catalytic Reduction of NO by C4 H6 over Calcined Hydrotalcite显示文摘 | Yuan Deling Li Xinyong Zhao Qidong | 2013 | Appl Catal A: General2013，451，： | 1 |
| 18 | Beta-arrestin 2 modulates resveratrol-induced apoptosis and regulation of Akt/GSK3β pathways显示文摘 | Xiuli Sun Yi Zhang Jianliu Wang Lihui Wei Hui Li Gregory Hanley Miaoqing Zhao Yi Li Deling Yin | 2010 | BBA - General Subjects2010，，9： | 1 |
| 19 | Synthesis Rheological Behavior and Mechanical Properties of Graft-type ACS Resin 显示文摘 | Jungang Gao Shuhui Sun Deling Li | 2002 | Polymer-Plastics Technology and Engineering2002，41，5： | 1 |
| 20 | Rheological Behavior and Mechanical Properties of Blends of Chlorinated Polyethylene with Poly ( acrylonitrile-styrene-methyl methacrylate) 显示文摘 | Jungang Gao Deling Li Dianye Wang | 2000 | European Polymer Journal2000，36，11： | 1 |
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