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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Deep Reinforcement Learning-Based Computation Offloading for 5G Vehicle-Aware Multi-Access Edge Computing Network显示文摘Multi-access Edge Computing(MEC)is one of the key technologies of the future 5G network.By deploying edge computing centers at the edge of wireless access network,the computation tasks can be offloaded to edge servers rather than the remote cloud server to meet the requirements of 5G low-latency and high-reliability application scenarios.Meanwhile,with the development of IOV(Internet of Vehicles)technology,various delay-sensitive and compute-intensive in-vehicle applications continue to appear.Compared with traditional Internet business,these computation tasks have higher processing priority and lower delay requirements.In this paper,we design a 5G-based vehicle-aware Multi-access Edge Computing network(VAMECN)and propose a joint optimization problem of minimizing total system cost.In view of the problem,a deep reinforcement learningbased joint computation offloading and task migration optimization(JCOTM)algorithm is proposed,considering the influences of multiple factors such as concurrent multiple computation tasks,system computing resources distribution,and network communication bandwidth.And,the mixed integer nonlinear programming problem is described as a Markov Decision Process.Experiments show that our proposed algorithm can effectively reduce task processing delay and equipment energy consumption,optimize computing offloading and resource allocation schemes,and improve system resource utilization,compared with other computing offloading policies. | Ziying Wu Danfeng Yan | 2021 | China Communications2021，18，11： | 9 |
| 3 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 4 | Whole-exome sequencing identifies a novel INS mutation causative of maturity-onset diabetes of the young 10显示文摘 | Jing Yan Feng Jiang Rong Zhang Tongfu Xu Zhou Zhou Wei Ren Danfeng Peng Yong Liu Cheng Hu Weiping Jia | 2017 | Journal of Molecular Cell Biology2017，9，5： | 7 |
| 5 | Construction of heparin-based hydrogel incorporated with Cu_(5.4)O ultrasmall nanozymes for wound healing and inflammation inhibition显示文摘Excessive production of inflammatory chemokines and reactive oxygen species(ROS)can cause a feedback cycle of inflammation response that has a negative effect on cutaneous wound healing.The use of wound-dressing materials that simultaneously absorb chemokines and scavenge ROS constitutes a novel‘weeding and uprooting’treatment strategy for inflammatory conditions.In the present study,a composite hydrogel comprising an amine-functionalized star-shaped polyethylene glycol(starPEG)and heparin for chemokine sequestration as well as Cu_(5.4)O ultrasmall nanozymes for ROS scavenging(Cu_(5.4)O@Hep-PEG)was developed.The material effectively adsorbs the inflammatory chemokines monocyte chemoattractant protein-1 and interleukin-8,decreasing the migratory activity of macrophages and neutrophils.Furthermore,it scavenges the ROS in wound fluids to mitigate oxidative stress,and the sustained release of Cu_(5.4)O promotes angiogenesis.In acute wounds and impaired-healing wounds(diabetic wounds),Cu_(5.4)O@Hep-PEG hydrogels outperform the standard-of-care product Promogram®in terms of inflammation reduction,increased epidermis regeneration,vascularization,and wound closure. | Yuan Peng Danfeng He Xin Ge Yifei Lu Yuanhao Chai Yixin Zhang Zhengwei Mao Gaoxing Luo Jun Deng Yan Zhang | 2021 | Bioactive Materials2021，6，10： | 7 |
| 6 | Plant buffering against the high-light stress- induced accumulation of CsGA2ox8 transcripts via alter native splici ng to fi n ely tune gibberelli n levels and maintain hypocotyl elongation显示文摘In plants,alternative splicing(AS)is markedly induced in response to environmental stresses,but it is unclear why plants generate multiple transcripts under stress conditions.In this study,RNA-seq was performed to identify AS events in cucumber seedlings grown under different light intensities.We identi fi ed a novel transcript of the gibberellin(GA)-deactivating enzyme Gibberellin 2-beta-dioxygenase 8(CsGA2ox8).Compared with canonical CsGA2ox8.1,the CsGA2ox8.2 isoform presented intron retention between the second and third exons.Functional analysis proved that the transcript of CsGA2ox8.1 but not CsGA2ox8.2 played a role in the deactivation of bioactive GAs.Moreover,expression analysis demonstrated that both transcripts were upregulated by increased light intensity,but the expression level of CsGA2ox8.1 increased slowly when the light intensity was>400μmol-m-2-s-1 PPFD(photosynthetic photon fl ux density),while the CsGA2ox8.2 transcript levels increased rapidly when the light intensity was>200 pmol-m-2-s-1 PPFD.Our fi ndings provide evidence that plants might fi nely tune their GA levels by buffering against the normal transcripts of CsGA2ox8 through AS. | Bin Liu Shuo Zhao Pengli Li Yilu Yin Qingliang Niu Jinqiang Yan Danfeng Huang | 2021 | Horticulture Research2021，8，1： | 2 |
| 7 | Cytoplasmic and nuclear genome variations of rice hybrids and their parents inform the trajectory and strategy of hybrid rice breeding显示文摘The male sterility(MS)line is a prerequisite for efficient production of hybrid seeds in rice,a self-pollinating species.MS line breeding is pivotal for hybrid rice improvement.Understanding the historical breeding tra-jectory will help to improve hybrid rice breeding strategies.Maternally inherited cytoplasm is an appro-priate tool for phylogenetic reconstruction and pedigree tracing in rice hybrids.In this study,we analyzed the cytoplasmic genomes of 1495 elite hybrid rice varieties and identified five major types of cytoplasm,which correspond to different hybrid production systems.As the cytoplasm donors for hybrids,461 MS lines were also divided into five major types based on cytoplasmic and nuclear genomic architecture.Spe-cific core accessions cooperating with different fertility-associated genes drove the sequence divergence of MS lines.Dozens to hundreds of convergent and divergent selective sweeps spanning several agro-nomic trait-associated genes were identified among different types of MS lines.We further analyzed the cross patterns between different types of MS lines and their corresponding restorers.This study system-atically analyzed the cytoplasmic genomes of rice hybrids revealed their relationships with nuclear ge-nomes of MS lines,and illustrated the trajectory of hybrid rice breeding and the strategies for breeding different types of MS lines providing new insights for future improvement of hybrid rice. | Zhoulin Gu Zhou Zhu Zhen Li Qilin Zhan Qi Feng Congcong Zhou Qiang Zhao Yan Zhao Xiaojian Peng Bingxin Dai Rongrong Sun Yan Li Hengyun Lu Lei Zhang Tao Huang Junyi Gong Danfeng Lv Xuehui Huang Bin Han | 2021 | Molecular Plant2021，14，12： | 2 |
| 8 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 9 | Study on test instrument and filtration theory of the carbonized micron wood fiber DPF 显示文摘 | Guo Xiurong Du Danfeng Wang Fenghu Ma Yan | 2011 | Microporous and Mesoporous Materials2011，142，23： | 1 |
| 10 | Trust-Compensation-Based Access Control Model for Web Services显示文摘For most current Web Service access control methods,Web Service providers create a series of access control rules based on specified attributes.Only by meeting all the rules can a subject obtain the access to necessary operations and resources.However,because of the dynamic and open traits of Web Services,it is difficult for Web Service providers to work out an access control policy with moderate intensity and to realize a satisfactory balance between protecting the security of resources and maintaining the service reachable rate.To provide a solution to the above problem,this paper proposed a trust compensation access control method based on the Attribute-Based Access Control model.Our main contributions include a formal description of the access control method,a method to calculate the attribute trust degree based on time decay,and the trust compensation value of the attribute trust degree,as well as a new Service Oriented Architecture(SOA) architecture and its procedures based on a detailed trust compensation access control method. | Yan Danfeng Sun Jing Zhang Liying Yang Fangchun | 2012 | China Communications2012，9，12： | 1 |
| 11 | Multi-label text classification model based on semantic embedding显示文摘Text classification means to assign a document to one or more classes or categories according to content. Text classification provides convenience for users to obtain data. Because of the polysemy of text data, multi-label classification can handle text data more comprehensively. Multi-label text classification become the key problem in the data mining. To improve the performances of multi-label text classification, semantic analysis is embedded into the classification model to complete label correlation analysis, and the structure, objective function and optimization strategy of this model is designed. Then, the convolution neural network(CNN) model based on semantic embedding is introduced. In the end, Zhihu dataset is used for evaluation. The result shows that this model outperforms the related work in terms of recall and area under curve(AUC) metrics. | Yan Danfeng Ke Nan Gu Chao Cui Jianfei Ding Yiqi | 2019 | The Journal of China Universities of Posts and Telecommunications2019，26，1： | 1 |
| 12 | An Early Stage Detecting Method against SYN Flooding Attacks显示文摘Existing detection methods against SYN flooding attacks are effective only at the later stages when attacking signatures are obvious.In this paper an early stage detecting method(ESDM) is proposed.The ESDM is a simple but effective method to detect SYN flooding attacks at the early stage.In the ESDM the SYN traffic is forecasted by autoregressive integrated moving average model, and non-parametric cumulative sum algorithm is used to find the SYN flooding attacks according to the forecasted traffic.Trace-driven simulations show that ESDM is accurate and efficient to detect the SYN flooding attacks. | Sun Qibo Wang Shangguang Yan Danfeng Yang Fangchun | 2009 | China Communications2009，6，4： | 1 |
| 13 | Targeted gene panel provides advantages over whole-exome sequencing for diagnosing obesity and diabetes mellitus显示文摘A small fraction of patients diagnosed with obesity or diabetes mellitus has an underlying monogenic cause.Here,we constructed a targeted gene panel consisting of 83 genes reported to be causative for monogenic obesity or diabetes.We performed this panel in 481 patients to detect causative variants and compared these results with whole-exome sequencing(WES)data available for 146 of these patients.The coverage of targeted gene panel sequencing was significantly higher than that of WES.The diagnostic yield in patients sequenced by the panel was 32.9%with subsequent WES leading to three additional diagnoses with two novel genes.In total,178 variants in 83 genes were detected in 146 patients by targeted sequencing.Three of the 178 variants were missed by WES,although the WES-only approach had a similar diagnostic yield.For the 335 samples only receiving targeted sequencing,the diagnostic yield was 32.2%.In conclusion,taking into account the lower costs,shorter turnaround time,and higher quality of data,targeted sequencing is a more effective screening method for monogenic obesity and diabetes compared to WES.Therefore,this approach could be routinely established and used as a first-tier test in clinical practice for specific patients. | Hairong Yu Haoyong Yu Rong Zhang Danfeng Peng Dandan Yan Yunjuan Gu Yuqian Bao Weiping Jia Hong Zhang Cheng Hu | 2023 | Journal of Molecular Cell Biology2023，15，6： | 0 |
| 14 | Illumination robust image transformations for feature-based SLAM using photometric and feature matches loss显示文摘Simultaneous localization and mapping(SLAM) technology becomes more and more important in robot localization. The purpose of this paper is to improve the robustness of visual features to lighting changes and increase the recall rate of map re-localization under different lighting environments by optimizing the image transformation model. An image transformation method based on matches and photometric error(name the method as MPT) is proposed in this paper, and it is seamlessly integrated into the pre-processing stage of the feature-based visual SLAM framework. The results of the experiment show that the MPT method has a better matching effect on different visual features. In addition, the image transformation module encapsulated by a robot operating system(ROS) can be used with multiple visual SLAM systems and improve its re-localization effect under different lighting environments. | Zhang Miao Wang Zixian Yan Danfeng | 2022 | The Journal of China Universities of Posts and Telecommunications2022，29，3： | 0 |
| 15 | ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development显示文摘Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation. | Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei Tingwei Yan Yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu Yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-NanYang Xiangyuan Wan | 2023 | Molecular Plant2023，16，8： | 0 |
| 16 | DRO-SLAM: real-time object-aware SLAM for navigation robots and autonomous driving in dynamic environments显示文摘Traditional simultaneous localization and mapping(SLAM) mostly performs under the assumption of an ideal static environment, which is not suitable for dynamic environments in the real world. Dynamic real-time object-aware SLAM(DRO-SLAM) is proposed in this paper, which is a visual SLAM that can realize simultaneous localizing and mapping and tracking of moving objects indoor and outdoor at the same time. It can use target recognition, oriented fast and rotated brief(ORB) feature points, and optical flow assistance to track multi-target dynamic objects and remove them during dense point cloud reconstruction while estimating their pose. By verifying the algorithm effect on the public dataset and comparing it with other methods, it can be obtained that the proposed algorithm has certain guarantees in real-time and accuracy, it also provides more functions. DRO-SLAM can provide the solution to automatic navigation which can realize lightweight deployment, provide more vehicles, pedestrians and other environmental information for navigation. | Wang Zixian Zhang Miao Yan Danfeng | 2023 | The Journal of China Universities of Posts and Telecommunications2023，30，3： | 0 |
| 17 | Recent advances in the development of in vitro liver models for hepatotoxicity testing显示文摘Liver injury is a common cause of drug approval withdrawal during drug development,pre-clinical research,and clinical treatment.If not properly treated,patients with severe liver injury can suffer from acute liver failure or even death.Thus,utilization of the convenient in vitro hepatotoxicity assessment model for early detection of drug-induced hepatotoxicity is vital for drug development and safe personalized medication.Biomaterials(e.g.,hydrogels,nanofibers,decellularized liver matrix)and bioengineering technologies(e.g.,microarrays,micropatterns,3D printing,and microfluidics)have been applied for in vitro hepatotoxicity assessment models.This review summarizes the structure and functions of the liver as well as the components of in vitro hepatotoxicity assessment models.In addition,it highlights the latest advances in developing hepatotoxicity models with the ultimate goal of further clinical translation. | Siming Lu Jingwei Zhang Sha Lin Danfeng Zheng Yan Shen Jiale Qin Yangyang Li Shuqi Wang | 2021 | Bio-Design and Manufacturing2021，4，4： | 0 |
| 18 | Fabrication and characterization of multi-scale coated boron powders with improved combustion performance:A brief review显示文摘Boron has high mass and volume calorific values,but it is difficult to ignite and has low combustion efficiency.This literature review summarizes the strategies that are used to solve the above-mentioned problems,which include coatings of boron by using fluoride compounds,energetic composites,metal fuels,and metal oxides.Coating techniques include recrystallization,dual-solvent,phase transfer,electrospinning,etc.As one of the effective coating agents,the fluorine compounds can react with the oxide shell of boron powder.In comparison,the energetic composites can effectively improve the flame temperature of boron powder and enhance the evaporation efficiency of oxide film as a condensed product.Metals and metal oxides would react with boron powder to form metal borides with a lower ignition point,which could reduce its ignition temperature. | Rui Liu Danfeng Yang Kunyu Xiong Ying-Lei Wang Qi-Long Yan | 2024 | Defence Technology(防务技术)2024，31，1： | 0 |
| 19 | A domestication-associated gene, CsLH, encodes a phytochrome B protein that regulates hypocotyl elongation in cucumber显示文摘Introduction Plant height is an important agronomic trait;tall plants are prone to collapse(lodging)and are unsuitable for high-density planting(Li et al.,2020).During the Green Revolution,a multitude of genes acting as core or peripheral regulators of plant height were identified and used in breeding(Eshed and Lippman,2019);however,most were reported in cereal crop plants(Eshed and Lippman,2019)and few have been characterized in the Cucurbitaceae,which are economically important horticultural plants cultivated worldwide.Here,we describe LONG HYPOCOTYL(CsLH),encoding the photoreceptor phytochrome B(PHYB),which we show has been subjected to selection during cucumber(Cucumis sativus L.)domestication. | Bin Liu Jinyang Weng Dailu Guan Yan Zhang Qingliang Niu Enrique López-Juez Yunsong Lai Jordi Garcia-Mas Danfeng Huang | 2021 | Molecular Horticulture2021，1，1： | 0 |
| 20 | A New Advance on Camellia Breeding in Chine. Studies on Thirty Summer-blooming Hybrids from Crosses between Camellia azalea and Other Camellias显示文摘 | Xinkai Liu Naisheng Zhong Guimei Feng Danfeng Yan Jiyin Gao | 2013 | Journal of Agricultural Science and Technology(B)2013，3，4： | 0 |
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