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| 1 | Cancer stem-like cells directly participate in vasculogenic mimicry channels in triple-negative breast cancer显示文摘Objective: Vasculogenic mimicry(VM) channels that are lined by tumor cells are a functional blood supply in malignant tumors.However, the role of VM-initiating cells remains poorly understood. Cancer stem-like cells(CSCs) are positively correlated with VM. In this study, triple-negative breast cancer(TNBC) enriched with CSCs was used to investigate the relationship between VM and CSCs.Methods: The expression of several CSC markers was detected by immunohistochemistry in 100 human breast cancer samples.The clinical significance of CSC markers and the relationship between VM, CSCs, breast cancer subtypes, and VM-associated proteins were analyzed. CD133+ and ALDH+ human and mouse TNBC cells were isolated by FACS to examine the ability of VM formation and the spatial relationship between VM and CSCs.Results: CSCs were associated with TNBC subtype and VM in human invasive breast cancer. CSCs in TNBC MDA-MB-231 cells formed more VM channels and expressed more molecules promoting VM than the non-TNBC MCF-7 cells in vitro. MDA-MB-231 cells that encircled VM channels on Matrigel expressed CD133. Moreover, CSCs were located near VM channels in the 3D reconstructed blood supply system in human TNBC grafts. The CD133+ and ALDH+ cells isolated from TA2 mouse breast cancer formed more VM channels in vivo.Conclusions: CSCs line VM channels directly. Additionally, CSCs provide more VM-related molecules to synergize VM formation. The signaling pathways that control CSC differentiation may also be potential treatment targets for TNBC. | Huizhi Sun Nan Yao Siqi Cheng Linqi Li Shiqi Liu Zhao Yang Guanjie Shang Danfang Zhang Zhi Yao | 2019 | Cancer Biology & Medicine2019，16，2： | 5 |
| 2 | Hypoxic microenvironment induced spatial transcriptome changes in pancreatic cancer显示文摘Objective: Hypoxia is a significant feature of solid tumors, including pancreatic ductal adenocarcinoma(PDAC). It is associated with tumor invasion, metastasis, and drug resistance. However, the spatial distribution of hypoxia-related heterogeneity in PDAC remains unclear.Methods: Spatial transcriptomics(STs), a new technique, was used to investigate the ST features of engrafted human PDAC in the ischemic hind limbs of nude mice. Transcriptomes from ST spots in the hypoxic tumor and the control were clustered using differentially-expressed genes. These data were compared to determine the spatial organization of hypoxia-induced heterogeneity in PDAC. Clinical relevance was validated using the Tumor Cancer Genome Atlas and KM-plotter databases. The CMAP website was used to identify molecules that may serve as therapeutic targets for PDAC.Results: ST showed that the tumor cell subgroups decreased to 7 subgroups in the hypoxia group, compared to 9 subgroups in the control group. Different subgroups showed positional characteristics and different gene signatures. Subgroup 6 located at the invasive front showed a higher proliferative ability under hypoxia. Subgroup 6 had active functions including cell proliferation, invasion, and response to stress. Expressions of hypoxia-related genes, LDHA, TPI1, and ENO1, induced changes. CMAP analysis indicated that ADZ-6482, a PI3 K inhibitor, was targeted by the invasive subgroup in hypoxic tumors.Conclusions: This study is the first to describe hypoxic microenvironment-induced spatial transcriptome changes in PDAC, and to identify potential treatment targets for PDAC. These data will provide the basis for further investigations of the prognoses and treatments of hypoxic tumors. | Huizhi Sun Danfang Zhang Chongbiao Huang Yuhong Guo Zhao Yang Nan Yao Xueyi Dong Runfen Cheng Nan Zhao Jie Meng Baocun Sun Jihui Hao | 2021 | Cancer Biology & Medicine2021，18，2： | 2 |
| 3 | The influence of different microenvironments on melanoma invasiveness and microcirculation patterns: an animal experiment study in the mouse model 显示文摘 | Baocun Sun Shiwu Zhang Danfang Zhang | 2007 | Cancer Res Clin Oncol2007，133，12： | 1 |
| 4 | Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells显示文摘 | SUN Tao SUN Baocun NI Chunsheng ZHAO Xiulan WANG Xinghui QIE Shuo ZHANG Danfang GU Qiang QI Hong ZHAO Nan | 2008 | Cancer Lett2008，263，1： | 1 |
| 5 | VEGFR2-Mediated Vascular Dilation as a Mechanism of VEGF-Induced Anemia and Bone Marrow Cell Mobilization显示文摘 | Sharon Lim Yin Zhang Danfang Zhang Fang Chen Kayoko Hosaka Ninghan Feng Takahiro Seki Patrik Andersson Jingrong Li Jingwu Zang Baocun Sun Yihai Cao | 2014 | Cell Reports2014，，： | 1 |
| 6 | Vasculogenic mimicry is associated with high tumor grade, invasion andmetastasis, and short survival in patients with hepatocellular carcinoma显示文摘 | Baocun Sun Shiwu Zhang Danfang Zhang Jing Du Hua Guo Xiulan Zhao Wei Zhang Xishan Hao | 2006 | Oncology Reports2006，，4： | 1 |
| 7 | The influence of different microenvironments on melanoma invasiveness and microcirculation patterns: an animal experiment study in the mouse model显示文摘 | Baocun Sun Shiwu Zhang Danfang Zhang Yanjun Gu Wenchao Zhang Xiulan Zhao | 2007 | Journal of Cancer Research and Clinical Oncology2007，，12： | 1 |
| 8 | Acyl-biotinyl Exchange Chemistry and Mass Spectrometry-Based Analysis of Palmitoylation Sites of In Vitro Palmitoylated Rat Brain Tubulin显示文摘 | Zhiqiang Zhao Junjie Hou Zhensheng Xie Jianwei Deng Xiaoming Wang Danfang Chen Fuquan Yang Weimin Gong | 2010 | The Protein Journal2010，，8： | 1 |
| 9 | Predic- tors for replanning in loco-regionally advanced nasopha- ryngeal carcinoma patients undergoing intensity-modulat- ed radiation therapy: A prospective observational study显示文摘 | Danfang Yan Senxiang Yan Qidong Wang | 2013 | BMC Cancer2013，13，4： | 1 |
| 10 | The influence of different microenvironments on melanoma invasiveness and microcirculation patterns: an animal experiment study in the mouse model显示文摘 | Baocun Sun Shiwu Zhang Danfang Zhang Yanjun Gu Wenchao Zhang Xiulan Zhao | 2007 | Journal of Cancer Research and Clinical Oncology2007，，12： | 1 |
| 11 | ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment显示文摘Objective: Hypoxia is an important feature of pancreatic ductal adenocarcinoma(PDAC). Previously, we found that hypoxia promotes ENO1 expression and PDAC invasion. However, the underlying molecular mechanism was remains unclear.Methods: The relationship between ENO1 expression and clinicopathological characteristics was analyzed in 84 patients with PADC. The effects of CoCl2-induced hypoxia and ENO1 downregulation on the apoptosis, invasion, and proliferation of PDAC cells were evaluated in vitro and in vivo. Hypoxia-and ENO1-induced gene expression was analyzed by transcriptomic sequencing.Results: The prognosis of PDAC with high ENO1 expression was poor(P < 0.05). High ENO1 expression was closely associated with histological differentiation and tumor invasion in 84 PDAC cases(P < 0.05). Hypoxia increased ENO1 expression in PDAC and promoted its migration and invasion. Apoptotic cells and the apoptosis marker caspase-3 in the CoCl_(2)-treated ENO1-sh group were significantly elevated(P < 0.05). Transcriptomic sequencing indicated that CoCl_(2)-induced PDAC cells initiated MAPK signaling. Under hypoxic conditions, PDAC cells upregulated ENO1 expression, thereby accelerating ERK phosphorylation and inhibiting apoptosis(P < 0.05). Consistent results were also observed in a PDAC-bearing mouse hindlimb ischemia model.Conclusions: Hypoxia-induced ENO1 expression promotes ERK phosphorylation and inhibits apoptosis, thus leading to PDAC survival and invasion. These results suggest that ENO1 is a potential therapeutic target for PDAC. | Huizhi Sun Jing Mo Runfen Cheng Fan Li Yue Li Yuhong Guo Yanlei Li Yanhui Zhang Xiaoyu Bai Yalei Wang Xueyi Dong Danfang Zhang Jihui Hao | 2022 | Cancer Biology & Medicine2022，19，11： | 0 |
| 12 | Bilateral asymmetric tonic seizure in insuloopercular epilepsy:an anatomo-electroclinical study显示文摘Background:Insulo-opercular seizures are highly heterogeneous in seizure semiology and electrical features.Bilateral asymmetric limb posturing,as a classical pattern of supplementary sensorimotor area(SMA)seizure,also occurs in insulo-opercular epilepsy.This study was aimed to study the anatomo-electro-clinical correlations in bilateral asymmetric tonic seizures(BATS),in order to advance the understanding of insulo-opercular epilepsy.Methods:Eight patients with insulo-opercular epilepsy as confirmed by stereoelectroencephalography(SEEG)and manifesting BATS as the major ictal motor sign,in Guangdong Sanjiu Brain Hospital Epilepsy Center from 2014 to 2018,were employed in this study.The BATS of the patients were evaluated,and the semiologic features and concomitant intracerebral EEG changes were quantified.Then the variables were examined with Cluster Analysis,and the semiologic features were correlated with anatomic localization using the Kendall correlation test.Results:Of the 8 patients,the most frequent initial motor sign was bilateral asymmetric tonic posturing(62.5%).Facial tonic-clonic sign also had a high prevalence in the evolution of seizures(87.5%).The results of Cluster Analysis showed that the semiologic features were subdivided into two main groups,one group comprising exclusively BATS and the other including signs of focal tonic seizure,aura,focal limb tonic-clonic seizure(TCS),facial TCS,hypermotor behavior,eye movement,autonomic changes and generalized TCS.The BATS was strongly associated with the posterior long gyrus(PLG)of insula(t=0.732)and parietal operculum(t=1.000);the hypermotor behaviors were associated with the anterior long gyrus(ALG)(t=0.770);and the autonomic changes were associated with the anterior limiting sulcus(ALS)(t=0.734)and middle short gyrus(MSG)(t=0.700).Conclusions:The seizure semiology of insulo-opercular epilepsy is characterized,in temporal order,by BATS,with or without simultaneous hypermotor behaviors,and frequently ends up with facial tonic-clonic signs,which is different from that of the SMA seizure.The early spread network involving the posterior insular lobe and parietal operculum may contribute to this pattern of manifestation. | Xiangshu Hu Kaihui Li Xiao Wang Ping Yang Qinghua Tan Danfang Li Xiaobo Wang Fang Zeng Yang Jin Lingxia Fei Hua Li Junxi Chen Qiang Guo | 2020 | Acta Epileptologica2020，2，1： | 0 |
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