|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Role of the advanced glycation end products receptor in Crohn's disease inflammation显示文摘AIM:To investigate the level of mucosal expression and the involvement of the receptor for the advanced glycation end products(RAGE)in delayed apoptosis and tumor necrosis factor(TNF)-αproduction in Crohn’s disease(CD).METHODS:Surgical and endoscopic specimens from both inflamed and non-inflamed areas of the ileum and/or colon were collected from 20 and 14 adult CD patients,respectively,and used for the assessment of RAGE expression by means of immunohistochemistry and western blotting analysis.Normal tissues from 21 control subjects were used for comparison.The same polyclonal anti-human RAGE antibody(R and D System)was used in all experimental conditions.RAGE staining was quantized by a score including both the amount of positive cells and intensity of immunoreactivity;cellular pattern was also described.The effects of RAGE blocking on apoptotic rate and TNF-αproduction were investigated on immune cells freshly isolated from CD mucosa and incubated both with and without the muramyl dipeptide used as antigenic stimulus.Statistical analysis was performed via the test for trend,with regression models to account for intra-patient correlations.A 2-sided P<0.05 was considered significant.RESULTS:In inflamed areas,RAGE expression in both the epithelial and lamina propria compartments was higher than control tissues(P=0.001 and 0.021,respectively),and a cluster of positive cells were usually found in proximity of ulcerative lesions.Similar results were obtained in the lamina propria compartment of non-inflamed areas(P=0.025).The pattern of staining was membranous and granular cytosolic at the epithelial level,while in the lamina propria it was diffuse cytosolic.When evaluating the amount of protein expression by immunoblotting,a significant increase of both surface area and band intensity(P<0.0001 for both)was observed in CD inflamed areas compared to control tissue,while in non-inflamed areas a significant increase was found only for band intensity(P<0.005).Moreover,a significantly lower expression in noninflamed areas in comparison with inflamed areas was found for both surface area and band intensity(P<0.0006 for both).Finally,RAGE blocking largely affects both the apoptotic rate of mucosal cells(towards an increase in both non-inflamed and inflamed areas of P<0.001 and<0.0001,respectively)and TNF-αsecretion(towards a decrease in both non-inflamed and inflamed areas of P<0.05 and<0.01,respectively),mainly in the presence of antigenic stimulation.CONCLUSION:RAGE is up-regulated in CD,especially in inflamed areas,and it appears to play a role in the mechanisms involved in chronic inflammation. | Rachele Ciccocioppo Alessandro Vanoli Catherine Klersy Venerina Imbesi Vincenzo Boccaccio Rachele Manca Elena Betti Giuseppina Cristina Cangemi Elena Strada Roberta Besio Antonio Rossi Colomba Falcone Sandro Ardizzone Paolo Fociani Piergiorgio Danelli Gino Roberto Corazza | 2013 | World Journal of Gastroenterology2013，19，45： | 2 |
| 2 | Spinal anesthesia with hyperbaric levobupivacaine and ropivacaine for outpatient knee arthroscopy: a prospective, randomized, double-blind study显示文摘 | Cappelleri G Aldegheri G Danelli G | 2005 | Anesth Analg2005，101，6： | 1 |
| 3 | Spinal anesthesia with hyperbaric levobupivacaine and ropivacaine for outpatient knee arthroscopy:a prospective,randomized,double-blind study显示文摘 | Cappelleri G Aldegheri G Danelli G | 2005 | Anesth Analg2005，101，： | 1 |
| 4 | Spinal anestesia with Hyperbaric levobupivacaine and ropivacaine for outpatient knee arthroseopy:a prospective,randomized,Double-blind study显示文摘 | Cappelleri G Aldegheri G Danelli G | 2005 | Anesth Analg2005，101，： | 1 |
| 5 | Prospective randomized com- parison of ultrasound-guided and neurostimulation techniques for continuous interscalene brachial plexus block in patients undergoing coracoacromial ligament repair显示文摘 | Danelli G Bonarelli S Tognu A | 2012 | Br J Anaesth2012，108，6： | 1 |
| 6 | Mast cell/MDSC a liaison immuno- suppressive for tumor microenvironment 显示文摘 | Danelli L Frossi B Pucillo CE | 2015 | Oncoimmunology2015，4，10： | 1 |
| 7 | Intratheeal 2-ehloroprocaine for lower limb outpatient surgery: a prospective, randomized, double-blind, clinieal evaluation显示文摘 | Casati A Danelli G Berti M | 2006 | Anesth Analg2006，103，1： | 1 |
| 8 | Reassessing the HAROLD model: is the hemispheric asymmetry reduction in older adults a special case of compensatory-related utilisation of neural circuits? 显示文摘 | BERLINGERI M DANELLI L BOTTINI G | 2013 | Exp Brain Res2013，224，3： | 1 |
| 9 | A prospective, randomized comparison, between ultrasound and nerve stimulation guidance for multiple injection axillary bra chial plexus block 显示文摘 | Casati A Danelli G Baciarello M | 2007 | Anesthesiology2007，106，5： | 1 |
| 10 | A prospective,randomized comparison between ultrasound and nerve stimulationguidance for multiple injection axillary brachial plexus block显示文摘 | Casati A Danelli G Baciarello M | 2007 | Anesthesiology2007，106，5： | 1 |
| 11 | Five-Aminosalicylic Acid: An Update for the Reappraisal of an Old Drug显示文摘 | Cristiana Perrotta Paolo Pellegrino Eliana Moroni Clara De Palma Davide Cervia Piergiorgio Danelli Emilio Clementi Bj?rn Moum | 2015 | Gastroenterology Research and Practice2015，，： | 1 |
| 12 | Prospective ran- domized comparison of ultrasound-guided and neurostimula- tion techniques for continuous interscalene brachial plexus block in patients undergoing coracoaeromial ligament repair 显示文摘 | Danelli G Bonarelli S Tognfi A | 2012 | Br J Anaesth2012，108，6： | 1 |
| 13 | Spinal mlesthesia with lidocaine or preservative - free 2 - chlornprocaine for outpatient knee ardu'oscopy: a prnspective, randomized, double - blind comparison 显示文摘 | CASATI A FANELI I G DANELLI G | 2007 | A nesth Analg2007，104，4： | 1 |
| 14 | A prospective,randomized comparison between ultrasound and nerve stimulation guidance for multiple injection axillary brachial plexus block显示文摘 | Casati A Danelli G Baciarello M | 2007 | Anesthesiolog- y2007，106，5： | 1 |
| 15 | A prospective, randomized comparison between ultrasound and nerve stimulation guidance for multiple injection axillary brachial plexus block 显示文摘 | CASATI A DANELLI G BACIARELLO M | 2007 | Anesthesiology2007，106，5： | 1 |
| 16 | Spinal anesthesia with hyperbaric levobupivacaine and ropivacaine for outpatient knee arthroscopy: a prospective, randomized, double-blind study显示文摘 | Cappelleri G Aldegheri G Danelli G | 2005 | Anesth Analg2005，101，1： | 1 |
| 17 | Spinal ropivacaine or bupivacaine for cesarean delivery:a prospective,randomized,doubleblind comparison显示文摘 | Danelli G F anelli G Berti M | | 0，，： | 1 |
| 18 | Spinal anesthesia with hyperbaric levobupivaeaine and ropivaeaine for outpatient knee arthroscopy : a prospective, randomized, double - blind study显示文摘 | CAPPELLERI G ALDEGHERI G DANELLI G | 2005 | Anesth Analg2005，101，1： | 1 |
| 19 | Prospective randomized comparison of ultrasound-guided and neurostimulation techniques for continuous interscalene brachial plexus block in patients undergoing coracoacromial ligament repair 显示文摘 | Danelli G Bonarelli S Tognff A | 2012 | Br J Anaesth2012，108，6： | 1 |
| 20 | A prospective, randomized comparison between ultrasound and nerve stimulation guidance for multiple injection axillary brachial plexus block显示文摘 | Casati A Danelli G Baciarello M | 2007 | Anesthesiology2007，106，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员