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| 1 | 多接收电感耦合等离子体质谱法高精密度测定汞同位素组成显示文摘采用多接收等离子体质谱(MC-ICP-MS)方法高精度测定了Hg同位素组成。本方法借助在线进样系统,最大程度上克服了同位素干扰和基体效应;采用同位素内标法和样品-标准交叉法以消除仪器自身的质量分馏;通过实验条件(如测定时间、进样量等)的优化,方法的内精度显著提高。研究表明:为保证汞同位素组成的高精度测定,汞最低进样浓度为2μg/L时,内精度<0.02‰(RSD)。运用本方法对汞标准NISTSRM3133和UM-Almadn实验室内标样长达7个月的测定,结果表明,本方法外精度<0.06‰(2SD)。此外,对一系列环境样品的同位素组成进行了测定,样品的外精度<0.10‰(2SD)。测定样品δ202Hg变化范围为-3.48‰~0.63‰,幅度达4.11‰。 | 尹润生 冯新斌 Foucher Delphine 侍文芳 赵志琦 王静 | 2010 | 分析化学2010，38，7： | 26 |
| 2 | Turning rice meiosis into mitosis显示文摘通过在庄稼的种子(单性生殖) 的同种细胞的复制的介绍有潜力由允许任何精英变化的自我繁殖革命化农业,特别地 F1 混血儿。在性模型植物 Arabidopsis thaliana,通过种子的合成同种细胞的复制能被联合三个变化把成熟分裂变成有丝分裂(笑剧) 并且(的(i) 人工地实现 ii ) 穿过获得的同种细胞的配偶子,一根线表示修改了 CENH3 并且其染色体在接合子被消除。这里,我们证明变化的另外的联合能把 Arabidopsis 成熟分裂变成有丝分裂并且在米饭(Oryza sativa ) 的三个变化的联合高效地把成熟分裂变成有丝分裂,导致在这主要庄稼的男、女的同种细胞的双配偶子的生产。在种系发生地远的 eudicot Arabidopsis 和 monocot 米饭的笑剧技术的成功的实现为它的申请打开门到任何 flowering 植物并且为在庄稼种类介绍单性生殖铺平道路。 | Delphine Mieulet Sylvie Jolivet Maud Rivard Laurence Cromer Aurore Vemet Pauline Mayonove Lucie Pereira Gaetan Droc Brigitte Courtois Emmanuel Guiderdoni Raphael Mercier | 2016 | Cell Research2016，26，11： | 14 |
| 3 | Microbial markers in colorectal cancer detection and/or prognosis显示文摘Colorectal cancer(CRC) is the second leading cause of cancer worldwide. CRC is still associated with a poor prognosis among patients with advanced disease. On the contrary, due to its slow progression from detectable precancerous lesions, the prognosis for patients with early stages of CRC is encouraging. While most robust methods are invasive and costly, actual patient-friendly screening methods for CRC suffer of lack of sensitivity and specificity. Therefore, the development of sensitive, non-invasive and cost-effective methods for CRC detection and prognosis are necessary for increasing the chances of a cure. Beyond its beneficial functions for the host, increasing evidence suggests that the intestinal microbiota is a key factor associated with carcinogenesis. Many clinical studies have reported a disruption in the gut microbiota balance and an alteration in the faecal metabolome of CRC patients, suggesting the potential use of a microbialbased test as a non-invasive diagnostic and/or prognostic tool for CRC screening. This review aims to discuss the microbial signatures associated with CRC known to date, including dysbiosis and faecal metabolome alterations, andthe potential use of microbial variation markers for noninvasive early diagnosis and/or prognostic assessment of CRC and advanced adenomas. We will finally discuss the possible use of these markers as predicators for treatment response and their limitations. | Romain Villéger Amélie Lopès Julie Veziant Johan Gagnière Nicolas Barnich Elisabeth Billard Delphine Boucher Mathilde Bonnet | 2018 | World Journal of Gastroenterology2018，24，22： | 10 |
| 4 | Type I interferons promote the survival and proinflammatory properties of transitional B cells in systemic lupus erythematosus patients显示文摘A hallmark of systemic lupus erythematosus(SLE)is the breaking of B-cell tolerance with the generation of high-affinity autoantibodies;however,the antibody-independent features of the B-cell compartment in SLE are less understood.In this study,we performed an extensive examination of B-cell subsets and their proinflammatory properties in a Chinese cohort of new-onset SLE patients.We observed that SLE patients exhibited an increased frequency of transitional B cells compared with healthy donors and rheumatoid arthritis patients.Plasma from SLE patients potently promoted the survival of transitional B cells in a type I IFN-dependent manner,which can be recapitulated by direct IFN-αtreatment.Furthermore,the effect of IFN-αon enhanced survival of transitional B cells was associated with NF-κB pathway activation and reduced expression of the pro-apoptotic molecule Bax.Transitional B cells from SLE patients harbored a higher capacity to produce proinflammatory cytokine IL-6,which was also linked to the overactivated type I IFN pathway.In addition,the frequency of IL-6-producing transitional B cells was positively correlated with disease activity in SLE patients,and these cells were significantly reduced after short-term standard therapies.Thus,the current study provides a direct link between type I IFN pathway overactivation and the abnormally high frequency and proinflammatory properties of transitional B cells in active SLE patients,which contributes to the understanding of the roles of type I IFNs and B cells in the pathogenesis of SLE. | Mei Liu Qiang Guo Chunmei Wu Delphine Sterlin Shyamal Goswami Ying Zhang Teng Li Chunde Bao Nan Shen Qiong Fu Xiaoming Zhang | 2019 | Cellular & Molecular Immunology2019，16，4： | 9 |
| 5 | 小麦挥发物对蚜虫及其天敌的行为影响——田间有效挥发性信息化合物的筛选(英文)显示文摘【目的】植物挥发性信息化合物能够影响植食性昆虫及其他天敌行为,因此,在植物-植食性昆虫-天敌互作系统中具有重要地位。目前,小麦[Triticum aestivum L.(Gramineae)]中多种挥发物已被鉴定,为明确其对蚜虫及其天敌的行为影响。【方法】本试验利用四臂嗅觉仪和风洞测定4种不同浓度的小麦挥发物(水杨酸甲酯、3-己酰醋酸酯、己烯醇和1-己醇)对麦长管蚜Sitobion avenae(Fabricus)及其两种重要天敌,异色瓢虫[Harmonia axyridis(Pallas)(Coleoptera:Coccinellidae)]和黑带食蚜蝇[(Episyrphus balteatus(De Geer)(Diptera:Syrphidae)]的行为反应。【结果】结果显示,水杨酸甲酯仅吸引异色瓢虫。3-己酰醋酸酯和己烯醇吸引麦长管蚜及异色瓢虫,但随其浓度增加对蚜虫的吸引作用降低,对异色瓢虫吸引作用增强。3-己酰醋酸酯对黑带食蚜蝇也具有吸引作用。另外,与天敌相比,1-己醇对麦长管蚜吸引作用更强。【结论】总之,本试验明确除1-己醇外,水杨酸甲酯、3-己酰醋酸酯、己烯醇可以作为吸引天敌,控制蚜虫的潜在用于推-拉策略的挥发物性化学信息物。 | 解海翠 Durieux Delphine 范佳 刘勇 Bragard Claude Haubruge Eric 孙京瑞 Francis Frédéric 陈巨莲 | 2014 | 应用昆虫学报2014，51，6： | 8 |
| 6 | Sampling variability of liver fibrosis in chronic hepatitis C显示文摘 | Pierre Bedossa Delphine Dargère Valerie Paradis | 2003 | Hepatology2003，，6： | 6 |
| 7 | New tools for optimizing fluid resuscitation in acute pancreatitis显示文摘Acute pancreatitis(AP)is a frequent disease with degrees of increasing severity responsible for high morbidity.Despite continuous improvement in care,mortality remains significant.Because hypovolemia,together with microcirculatory dysfunction lead to poor outcome,fluid therapy remains a cornerstone of the supportive treatment.However,poor clinical evidence actually support the aggressive fluid therapy recommended in recent guidelines since available data are controversial.Fluid management remains unclear and leads to current heterogeneous practice.Different strategies may help to improve fluid resuscitation in AP.On one hand,integration of fluid therapy in a global hemodynamic resuscitation has been demonstrated to improve outcomein surgical or septic patients.Tailored fluid administration after early identification of patients with high-risk of poor outcome presenting inadequate tissue oxygenation is a major part of this strategy.On the other hand,new decision parameters have been developed recently to improve safety and efficiency of fluid therapy in critically ill patients.In this review,we propose a personalized strategy integrating these new concepts in the early fluid management of AP.This new approach paves the way to a wide range of clinical studies in the field of AP. | Perrine Bortolotti Fabienne Saulnier Delphine Colling Alban Redheuil Sebastien Preau | 2014 | World Journal of Gastroenterology2014，20，43： | 5 |
| 8 | Clinical characteristics and outcomes of patients with hepatic angiomyolipoma:A literature review显示文摘First reported in 1976,hepatic angiomyolipoma(HAML)is a rare mesenchymal liver tumor occurring mostly in middle-aged women.Diagnosis of the liver mass is often incidental on abdominal imaging due to the frequent absence of specific symptoms.Nearly 10%of HAMLs are associated with tuberous sclerosis complex.HAML contains variable proportions of blood vessels,smooth muscle cells and adipose tissue,which renders radiological diagnosis hazardous.Cells express positivity for HMB-45 and actin,thus these tumors are integrated into the group of perivascular epithelioid cell tumors.Typically,a HAML appears on magnetic resonance imaging(or computed tomography scan)as a hypervascular solid tumor with fatty areas and with washout,and can easily be misdiagnosed as other liver tumors,particularly hepatocellular carcinoma.The therapeutic strategy is not clearly defined,but surgical resection is indicated for symptomatic patients,for tumors showing an aggressive pattern(i.e.,changes in size on imaging or high proliferation activity and atypical epithelioid pattern on liver biopsy),for large(>5 cm)biopsy-proven HAML,and if doubts remain on imaging or histology.Conservative management may be justified in other conditions,since most cases follow a benign clinical course.In summary,the correct diagnosis of HAML is challenging on imaging and relies mainly on pathological findings. | Paul Calame Gaelle Tyrode Delphine Weil Verhoeven Sophie Felix Anne Julia Klompenhouwer Vincent Di Martino Eric Delabrousse Thierry Thevenot | 2021 | World Journal of Gastroenterology2021，27，19： | 5 |
| 9 | 癌周及癌中心不同截短片段丙型肝炎病毒核心蛋白表达质粒的构建及表达显示文摘目的 研究不同截短片段丙型肝炎病毒(HCV)的核心蛋白(CORE)在HCV持续感染和肝癌发病机制中的作用,构建并表达不同HCV病毒株及不同截短片断CORE原核表达质粒。 方法 用PCR扩增基因型为HCV 1 b型的7个不同截短片段CORE基因:氨基酸(aa)长度分别为癌中心株(BT):B:1-172 aa、1-126 aa、1-58 aa、59-126 aa、127-172 aa;癌旁株(BNT):1-172 aa及C191(HCV-J6):1-172 aa,扩增产物用BamH Ⅰ及EcoR Ⅰ双酶切后将其插入到原核表达质粒pGEX-4T-1中,阳性克隆转染到BL21中,IPTG诱导表达GST-CORE融合蛋白,纯化定量,并用western blot加以验证。 结果 7个不同片段CORE在体外都得到相应表达,但表达量存在一定的差异,较长片段表达量相对较低,其中BT及BNT的1-172 aa截短片段CORE的表达高于同基因型等长度片段的C191,而BT以59-126 aa片段的表达量最高。BT、BNT及C191三株HCV基因1b型病毒株部分截短片段可形成二聚体。 结论 构建不同HCV病毒株及不同截短片段的CORE原核表达质粒获得成功,为研究HCV不同病毒株及不同区域CORE功能奠定基础;不同截短片段的CORE表达量不等,可能与不同片段具有不同的疏水基团、细胞毒性及在HCV结构和在HCV致病性中所起作用不同有关;HCV基因1b型的CORE二聚体的形成依耐于59-126 aa区域。 | 颜学兵 陈智 Delphine BOUCREUX Nicole PAVIO Christian BRECHOT | 2004 | 中华肝脏病杂志2004，12，11： | 5 |
| 10 | 光激酶C基因突变导致的大头精子畸形是阿尔及利亚男性不育症的最常见遗传因素显示文摘克氏综合征和Y染色体微缺失是过去针对男性不育症仅有的两项基因检测分析技术。北非男性经常出现两种罕见的畸形精子症:大头和圆头精子畸形,推荐进行AURKC和DPYl9L2基因检测。对阿尔及利亚男性不育患者进行以上基因检测,评估两种畸形精子症的患病率,并与克氏综合征和Y染色体微缺失的频率进行比较。我们对阿尔及利亚君士坦丁市的IbnRochd医院辅助生殖中心进行不育症咨询的599名患者进行了回顾性研究,其中404人存在精液参数异常现象。14人和7人分别患有典型的大头精子畸形或圆头精子畸形,进而对这些患者进行了AURKC和DPYl9L2基因分析显示,11名大头精子畸形患者存在4URKC基因突变(79%),相当于所有精液异常人群的2.7%。而5名圆头精子畸形患者,相当于1.2%的不育症患者,均存在DPYl9L2基因缺失。通过比较,我们预测该人群中克氏综合症和Y染色体微缺失的患病率分别是1.6%和0.23%。我们的研究表明AURKC基因突变较克氏综合症更常见,它是导致北非男性不育的主要遗传因素。此外,本文也预测了AURKC和DP"觋2基因缺陷发生率分别是Y染色体微缺失发生率的10倍和5倍。 | Leyla Ounis Abdelali Zoghmar Charles Coutton Leila Rouabah Maroua Hachemil Delphine Martinez Guillaume Martinez Ines Bellil Douadi Khelifi Christophe Arnoult Julien Faure Sebti Benbouhedja Abdelkader Rouabah Pierre F Ray | 2015 | Asian Journal of Andrology2015，17，1： | 5 |
| 11 | Defense-Related Calcium Signaling Mutants Uncovered via a Quantitative High-Throughput Screen in Arabidopsis thaliana显示文摘钙为包括 MAMP (联系微生物的分子的模式) 发信号到许多刺激充当第二个送信人,例如分别地从细菌的 flagellin 和延伸因素 Tu 被导出的 flg22 和 elf18。这里,有响应 flg22 处理的改变的钙举起(cce ) 的 Arabidopsis thaliana 异种被孤立并且描绘。除 flg22 受体的新奇变异的等位基因以外, FLS2 (Flagellin 敏感 2 ) ,并且联系受体的 kinase, BAK1 (Brassinosteroid 受体 1 伙伴 Kinase 1 ) ,新 cce 异种能被分成二主要 groupsthose 与一减少或提高的钙举起。而且,从两个组的 cce 异种显示出微分显型到 MAMP 的不同集合。因此,这些异种将在早 MAMP 发信号便于新奇部件的发现并且在钙在 plantmicrobe 相互作用期间发信号的当前的知识衔接差距。上次然而并非最不,屏蔽方法为速度被优化(盖住 384 在 3 的植物或 10h ) 并且能被改编遗传上把在钙层次导致一个变化的任何另外的刺激。 | Stefanie Ranf Julia Grimmer Yvonne P6schl Pascal Pecher Delphine Chinchilla Dierk Scheel Justin Lee | 2012 | Molecular Plant2012，5，1： | 5 |
| 12 | 减少丝光羊毛在染色中的损伤显示文摘阐述了染色时氯化丝光羊毛发生损伤的原因以及染色工艺条件对丝光羊毛损伤的影响。控制染色工艺参数可减少丝光羊毛染色时的损伤 ,交链类羊毛保护剂对丝光羊毛有较好的保护作用。通过正交实验得出 ,染色的pH值对丝光羊毛的强力影响最大 ,其次是保护剂 ,次之是染色的温度 ,最后是染色的时间。 | 蒋冶欣 朱泉 Roux Delphine | 2001 | 毛纺科技2001，29，2： | 5 |
| 13 | Noninvasive assessment of liver fibrosis with impulse elastography: comparison of Supersonic Shear Imaging with ARFI and Fibroscan显示文摘 | Christophe Cassinotto Bruno Lapuyade Amaury Mouries Jean-baptiste Hiriart Julien Vergniol Delphine Gaye Claire Castain Brigitte Le Bail Faiza Chermak Juliette Foucher Fran?ois Laurent Michel Montaudon Victor De Ledinghen | 2014 | Journal of Hepatology2014，，： | 4 |
| 14 | What neonatal complications should the pediatrician be aware of in case of maternal gestational diabetes?显示文摘In the epidemiologic context of maternal obesity and type 2 diabetes(T2D),the incidence of gestational diabetes has significantly increased in the last decades.Infants of diabetic mothers are prone to various neonatal adverse outcomes,including metabolic and hematologic disorders,respiratory distress,cardiac disorders and neurologic impairment due to perinatal asphyxia and birth traumas,among others.Macrosomia is the most constant consequence of diabetes and its severity is mainly influenced by maternal blood glucose level.Neonatal hypoglycemia is the main metabolic disorder that should be prevented as soon as possible after birth.The severity of macrosomia and the maternal health condition have a strong impact on the frequency and the severity of adverse neonatal outcomes.Pregestational T2 D and maternal obesity significantly increase the risk of perinatal death and birth defects.The high incidence of maternal hyperglycemia in developing countries,associated with the scarcity of maternal and neonatal care,seriously increase the burden of neonatal complications in these countries. | Delphine Mitanchez Catherine Yzydorczyk Umberto Simeoni | 2015 | World Journal of Diabetes2015，6，5： | 4 |
| 15 | Gastrointestinal Microbiome Signatures of Pediatric Patients With Irritable Bowel Syndrome显示文摘 | Delphine M. Saulnier Kevin Riehle Toni–Ann Mistretta Maria–Alejandra Diaz Debasmita Mandal Sabeen Raza Erica M. Weidler Xiang Qin Cristian Coarfa Aleksandar Milosavljevic Joseph F. Petrosino Sarah Highlander Richard Gibbs Susan V. Lynch Robert J. Shulman | 2011 | Gastroenterology2011，，5： | 4 |
| 16 | 丙型肝炎病毒核心蛋白结合蛋白激酶R的功能区域研究显示文摘目的构建并表达丙型肝炎病毒(HCV)不同病毒株:癌中心株(BT)、癌旁珠(BNT)、HCV-J及BT不同截短片段谷胱甘肽(GST)-核心融合蛋白;寻找核心蛋白(Core)与蛋白激酶R(PKR)相互作用区域,探讨它们在HCV持续感染及细胞癌(HCC)发病机制中的作用.方法用聚合酶链反应扩增不同片段HCV核心蛋白基因,并将7个不同的基因片段分别克隆到原核表达载体pGEX-4T-1,诱导表达并纯化表达蛋白,与两株细胞(HepG2和Huh-7)的PKR的进行相互作用试验.结果7个不同片段Core在体外都得到相应表达,不同片段结合PKR的能力存在一定差异.BT、BNT、C191的Core N端1~172氨基酸(aa)3个片段均能与PKP发生直接结合,BT与PKR结合的区域在Core N端的1~58aa.结论 不同片段Core在原核细胞中获得较好的表达;Core/PKP相互作,在HCV持续感染和HCC的发病机制中可能起重要作用. | 颜学兵 陈智 Delphine BOUCREUX Nicole PAVIO Christian BRECHOT | 2005 | 中华传染病杂志2005，23，1： | 4 |
| 17 | 疗效评价标准及其在淋巴瘤中的应用:第一部分显示文摘无论对于个体患者还是人群.癌症治疗的有效性都需要一种系统的、可重复的疗效评价方法。为满足这一需求,学界制定了一些评估疗效的指导原则,主要从解剖和代谢方面来量化治疗后的变化。过去几年针对特定的肿瘤组织制定了相应的疾病疗效分类标准迄今为止,恶性淋巴瘤可能是疾病疗效分类应用最普遍的肿瘤类型.该文对现有的淋巴瘤疗效评价标准进行概述,重点介绍这些评价标准各自的方法及其适用性。针对阻碍疗效评价标准化发展的问题,该文也对其中许多标准的技术复杂度和主观阈值判定的内容进行了评述。 | Mateen C.Moghbel Laie Kostakoglu Katherine Zukotynski Delphine L.Chen Helen Nadel Ryan Niederkohr Erik Mittra 李慧玲(译) 兰晓莉 刘振(审校) | 2019 | 中华核医学与分子影像杂志2019，39，3： | 3 |
| 18 | Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit显示文摘Improvement of nutritional and organoleptic quality of fruits is a key goal in current strawberry breeding programs.The ratio of sugars to acids is a determinant factor contributing to fruit liking,although different sugars and acids contribute in varying degrees to this complex trait.A segregating F1 population of 95 individuals,previously characterized for several fruit quality characters,was used to map during 2 years quantitative trait loci(QTL)for 50 primary metabolites,L-ascorbic acid(L-AA)and other related traits such as soluble solid content(SSC),titratable acidity(TA),and pH.A total of 133 mQTL were detected above the established thresholds for 44 traits.Only 12.9%of QTL were detected in the 2 years,suggesting a large environmental influence on primary metabolite content.An objective of this study was the identification of key metabolites that were associated to the overall variation in SSC and acidity.As it was observed in previous studies,a number of QTL controlling several metabolites and traits were co-located in homoeology group V(HG V).mQTL controlling a large variance in raffinose,sucrose,succinic acid,and L-AA were detected in approximate the same chromosomal regions of different homoeologous linkage groups belonging to HG V.Candidate genes for selected mQTL are proposed based on their co-localization,on the predicted function,and their differential gene expression among contrasting F1 progeny lines.RNA-seq analysis from progeny lines contrasting in L-AA content detected 826 differentially expressed genes and identified Mannose-6-phosphate isomerase,FaM6PI1,as a candidate gene contributing to natural variation in ascorbic acid in strawberry fruit. | JoséG.Vallarino Delphine M.Pott Eduardo Cruz-Rus Luis Miranda Juan J.Medina-Minguez Victoriano Valpuesta Alisdair R.Fernie JoséF.Sánchez-Sevilla Sonia Osorio Iraida Amaya | 2019 | Horticulture Research2019，6，1： | 3 |
| 19 | Liver Fibrosis: Noninvasive Assessment with Acoustic Radiation Force Impulse Elastography—Comparison with FibroScan M and XL Probes and FibroTest in Patients with Chronic Liver Disease显示文摘 | Christophe Cassinotto Bruno Lapuyade Ania A?t-Ali Julien Vergniol Delphine Gaye Juliette Foucher Claire Bailacq-Auder Faiza Chermak Brigitte Le Bail Victor de Lédinghen | 2013 | Radiology2013，，1： | 3 |
| 20 | Stability of highly supersaturated vanadium electrolyte solution and characterization of precipitated phases for vanadium redox flow battery显示文摘The vanadium redox flow battery(VRFB)has been receiving great attention in recent years as one of the most viable energy storage technologies for large-scale applications.However,higher concentrations of vanadium species are required in the H_(2)O-H_(2)SO_(4) electrolyte in order to improve the VRFB energy density.This might lead to unwanted precipitation of vanadium compounds,whose nature has not been accurately characterized yet.For this purpose,this study reports the preparation ofⅤ^((Ⅱ)),ⅤV^((Ⅲ)),Ⅴ^((Ⅳ))andⅤ^((Ⅴ))supersaturated solutions in a 5 M H_(2)SO_(4)-H_(2)O electrolyte by an electrolytic method,from the only vanadium sulfate compound commercially available(VOSO_(4)).The precipitates obtained by ageing of the stirred solutions are representative of the solids that may form in a VRFB operated with such supersaturated solutions.The solid phases are identified using thermogravimetric analysis,X-ray diffraction and SEM.We report that dissolvedⅤ^((Ⅱ)),Ⅴ^((Ⅲ))andⅤ^((Ⅳ))species precipitate as crystals of VSO_(4),V_(2)(SO_(4))3 and VOSO_(4) hydrates and not in their anhydrous form;conversely V^((Ⅴ))precipitates as an amorphous V_(2) O_(5) oxide partially hydrated.The measured hydration degrees(respectively 1.5,9,3 and 0.26 mol of H_(2)O per mol of compound)might significantly affect the overall engineering of VRFB operating with high vanadium concentrations. | Waldemir M.Carvalho Jr Laurent Cassayre Delphine Quaranta Fabien Chauvet Ranine El-Hage Theodore Tzedakis Béatrice Biscans | 2021 | Journal of Energy Chemistry2021，30，10： | 3 |
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