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| 1 | miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus显示文摘AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium. | Cameron M Smith David I Watson Mary P Leong George C Mayne Michael Z Michael Bas PL Wijnhoven Damian J Hussey | 2011 | World Journal of Gastroenterology2011，17，8： | 13 |
| 2 | MicroRNA profile in neosquamous esophageal mucosa following ablation of Barrett's esophagus显示文摘AIM To investigate the micro RNA expression profile in esophageal neosquamous epithelium from patients who had undergone ablation of Barrett's esophagus.METHODS High throughput screening using Taq Man~ Array Human Micro RNA quantitative PCR was used to determine expression levels of 754 micro RNAs in distal esophageal mucosa(1 cm above the gastro-esophageal junction) from 16 patients who had undergone ablation of non-dysplastic Barrett's esophagus using argon plasma coagulation vs pretreatment mucosa, posttreatment proximal normal non-treated esophageal mucosa, and esophageal mucosal biopsies from 10 controls without Barrett's esophagus. Biopsies of squamous mucosa were also taken from 5 cm above the pre-ablation squamo-columnar junction. Predicted m RNA target pathway analysis was used to investigate the functional involvement of differentially expressed micro RNAs.RESULTS Forty-four micro RNAs were differentially expressed between control squamous mucosa vs post-ablation neosquamous mucosa. Nineteen micro RNAs were differentially expressed between post-ablation neosquamous and post-ablation squamous mucosa obtained from the more proximal non-treated esophageal segment. Twelve microRNAs were differentially expressed in both neosquamous vs matched proximal squamous mucosa and neosquamous vs squamous mucosa from healthy patients. Nine micro RNAs(mi R-424-5p, mi R-127-3p, mi R-98-5p, mi R-187-3p, mi R-495-3p, mi R-34c-5p, mi R-223-5p, mi R-539-5p, mi R-376a-3p, mi R-409-3p) were expressed at higher levels in post-ablation neosquamous mucosa than in matched proximal squamous and healthy squamous mucosa. These micro RNAs were also more highly expressed in Barrett's esophagus mucosa than matched proximal squamous and squamous mucosa from controls. Target prediction and pathway analysis suggests that these micro RNAs may be involved in the regulation of cell survival signalling pathways. Three micro RNAs(mi R-187-3p, mi R-135b-5p and mi R-31-5p) were expressed at higher levels in postablation neosquamous mucosa than in matched proximal squamous and healthy squamous mucosa. These mi RNAs were expressed at similar levels in preablation Barrett's esophagus mucosa, matched proximal squamous and squamous mucosa from controls. Target prediction and pathway analysis suggests that these micro RNAs may be involved in regulating the expression of proteins that contribute to barrier function.CONCLUSION Neosquamous mucosa arising after ablation of Barrett's esophagus expresses micro RNAs that may contribute to decreased barrier function and micro RNAs that may be involved in the regulation of survival signaling pathways. | Loveena Sreedharan George C Mayne David I Watson Timothy Bright Reginald V Lord Alfiya Ansar Tingting Wang Jakob Kist David StJ Astill Damian J Hussey | 2017 | World Journal of Gastroenterology2017，23，30： | 3 |
| 3 | Bedside detection of awareness in the vegetative state: a cohort study显示文摘 | Damian C Srivas C Camilla C | 2011 | Lancet2011，378，9809： | 1 |
| 4 | Cross-neutralization of influenza A viruses mediated by a single antibody loop显示文摘 | Ekiert Damian C | 2012 | Nature2012，489，7417： | 1 |
| 5 | Multiscale Description of the Inhomogeneity of Multiphase Materials 显示文摘 | JERZY C DAMIAN S JANUSZ S | 2009 | Materials Characterization2009，60，： | 1 |
| 6 | Solid phase microextraction (SPME) combined with gas-chromatography and olfactometry-mass spectrometry for characterization of cheese aroma compounds显示文摘 | DAMIAN C F CAROLINE M O JOHN P | 2004 | LWT2004，37，2： | 1 |
| 7 | Towards automatic measure- ment of anteversion and neck-shaft angles in human femurs using CT images 显示文摘 | Casciaro ME Damian C | 2014 | Comput Methods Biomech Biomed Engin2014，17，2： | 1 |
| 8 | Mild cognitive impairment and deficits in instrumental activities of daily living: a systematic review显示文摘 | Jekel K Damian M Wattmo C | 2015 | Alzheimers Res Ther2015，7，1： | 1 |
| 9 | Measurements in the thick axisymmetric turbulent boundary layer near the tail of a body of revolution显示文摘 | Patel V C Nakayama A Damian R | 1974 | Journal of Fluid Mechanics1974，63，： | 1 |
| 10 | Turner' syndrome,diagnosis and therapeutical approach显示文摘 | Moraru E Dumitrache C Rusu T Damian A Rusu C Cernescu I | 2005 | Rev Med Chir Soc Med Nat Iasi2005，109，1： | 1 |
| 11 | Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus显示文摘BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many diseases.However,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA biomarkers.While small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been defined.AIM To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker studies.METHODS Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano Sight.RNA was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q PCR.The overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV preparations.The diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also compared.RESULTS The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV preparations.Specific blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct 20-25)was greater than serum s EV preparations(50%vs 31%).While the percentage of vesicle-associated mi RNAs expressed at relatively higher levels was greater in the serum s EV preparations than plasma s EV preparations(70%vs 44%).A 5-mi RNA biomarker panel produced a higher cross validated accuracy for discriminating patients with esophageal adenocarcinoma from healthy controls using serum s EV preparations compared with plasma s EV preparations(AUROC 0.80 vs 0.54,P<0.05).CONCLUSION Although plasma s EV preparations contained more mi RNAs than serum s EV preparations,they also contained more mi RNAs from non-vesicle origins.Serum appears to be more suitable than plasma for s EV mi RNAs biomarkers studies. | Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey | 2020 | World Journal of Gastroenterology2020，26，20： | 1 |
| 12 | High intensity focused ultrasound ablation of kidney guided by MRI显示文摘 | DAMIANOU C PAVLOU M VELEV O | 2004 | Ultrasound Med Biol2004，30，3： | 1 |
| 13 | ITS-RFLP and se- quence analysis of endophytes from Acianthus, Caladenia and Pterostylis (()rchidaceae) in southeastern Queensland 显示文摘 | Jeremy J B Damian S B John W G C | 2005 | Mycol Res2005，109，4： | 1 |
| 14 | The contribution of ultrasonography and sonoelastography in assessment of myositis显示文摘 | Botar-Jid C Damian L Dudea SM | 2010 | Medical Ultrasonography2010，12，2： | 1 |
| 15 | Precontoured Parallel Plate Fixation of AO/OTA Type C Distal Humerus Fractures显示文摘 | George S Athwal Samuel C Hoxie Damian M Rispoli Scott P Steinmann | 2009 | Journal of Orthopaedic Trauma2009，，8： | 1 |
| 16 | Managingand Sharing Servents'Reputations in P2P Systems显示文摘 | Damian E di Vimercati S D C Paraboschi S | 2003 | IEEETransactions on Data and Knowledge Engineering2003，15，4： | 1 |
| 17 | Oxygen tension modulates neu- rite outgrowth in PC12 cells through a mechanism involving HIF and VEGF显示文摘 | Damian C Genetos Whitney K | 2010 | J Mol Neurosci2010，40，360： | 1 |
| 18 | Percutaneous injection of thrombin for the treatment of pseudoaneurysms after catheterization: an alternative to sonographically guided compression显示文摘 | John A P Damian E D John J C | 2000 | American journal of Roentgenology2000，175，24： | 1 |
| 19 | Characterization of the timing and prevalence of receptor tyrosine kinase expression changes in oesophageal carcinogenesis显示文摘 | Anna L Paterson Maria O’Donovan Elena Provenzano Liam J Murray Helen G Coleman Brian T Johnson Damian T McManus Marco Novelli Laurence B Lovat Rebecca C Fitzgerald | 2013 | J Pathol2013，，1： | 1 |
| 20 | Histologic effects of high intensity pulsed ultrasound exposure with subharmonic emission in rabbit brain in vivo显示文摘 | Vykhodtseva NI Hynynen K Damianou C | 1995 | Ultrasound Med Biol1995，21，7： | 1 |
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