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| 1 | Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells显示文摘 | Shuya Zhou Chenhui Ding Xiaoyang Zhao Eryao Wang Xiangpeng Dai Lei Liu Wei Li Zichuan Liu Haifeng Wan Chunjing Feng Tang Hai Liu Wang Qi Zhou | 2010 | Cell Research2010，20，7： | 10 |
| 2 | Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle显示文摘Whether glucose is predomi nantly metabolized via oxidative phosphorylati on or glycolysis differs between quiesce nt versus proliferating cells,iincluding tumor cells.However,how glucose metabolism is coordinated with cell cycle in mammalian cells remains elusive.Here,we report that mammalian cells predominantly utilize the tricarboxylic acid(TCA)cycle in G1 phase,but prefer glycolysis in S phase.Mechanistically,coupling cell cycle with metabolism is largely achieved by timely destruction of IDH1/2,key TCA cycle enzymes,in a Skp2-dependent manner.As such,depleting SKP2 abolishes cell cycle-dependent fluctuation of IDH1 protein abundance,leading to reduced glycolysis in S phase.Furthermore,elevated Skp2 abundance in prostate cancer cells destabilizes IDH1 to favor glycolysis and subsequent tumorigenesis.Therefore,our study reveals a mechanistic link between two cancer hallmarks,aberrant cell cycle and addiction to glycolysis,and provides the underlying mechanism for the coupling of metabolic fluctuation with periodic cell cycle in mammalian cells. | Jing Liu Yunhua Peng Le Shi Lixin Wan Hiroyuki Inuzuka Jiangang Long Jianping Guo Jinfang Zhang Min Yuan Shuangxi Zhang Xun Wang Jing Gao Xiangpeng Dai Shozo Furumoto Lijun Jia Pier Paolo Pandolfic John M.Asara William G.Kaelin Jr. Jjankang Liu Wenyi Wei | 2021 | Cell Research2021，31，1： | 2 |
| 3 | Mouse cloning and somatic cell reprogramming using electrofused blastomeres显示文摘老鼠从受精卵克隆能为遗传上定制的人的胚胎的茎(ES ) 的生产帮助途径的开发没被卵母细胞可获得性的限制抑制的房间线。然而,成功地从导致 ES 房间线的严重地区分的体的房间施主被用作标明日期仅仅接合子原子核的接受者。在富饶诊所,先进胚胎的阶段的胚胎通常被存储为支持经由体的原子转移的房间线还没被证明了的 ES 的推导的未来使用,而是他们的能力。这里,我们报导那只二房间的阶段 electrofused 老鼠胚胎,在有丝分裂逮捕了,能从从分裂球到体的房间的施主房间支持原子核的发展 reprogramming。从胚胎的施主的实时、完整术语的克隆的小狗,以及从胚胎或体的施主的 pluripotent ES 房间线,成功地从这些重建的胚胎被产生。先进阶段培植前胚胎是不能的通常发展在 electrofusion 以后称为并且一个体的房间原子核转,显示丢弃的培植前人胚胎能是为最小化为人的治疗学的克隆的道德的担心的研究的一个重要资源。我们的途径提供一种吸引人、实际的选择给为病人特定的人的 ES 房间线的产生的用捐赠卵母细胞的治疗学的克隆。 | Amjad Riaz Xiaoyang Zha Xiangpeng Dai Wei Li Lei Liu Haifeng Wan Yang Yu Liu Wang Qi Zhou | 2011 | Cell Research2011，21，5： | 2 |
| 4 | Establishment of customized mouse stem cell lines by sequential nuclear transfer显示文摘治疗学的克隆,胚胎的干细胞(转换字符) 由此从原子转移(NT ) 被导出胚胎,可以在再生药的新时代起一个主要作用。Inthis 学习我们建立了四十原子转移 -- 从不同施主房间类型或段落的 NTembryos 被导出的转换字符(NT 转换字符) 线。我们发现 NT 转换字符能够形成胚胎植物或动物身体。另外, NT 转换字符表示了 pluripotency 干细胞标记试管内并且能区分进胚胎的纸巾体内。从施主房间能形成完整的术语的早经过 R1 的 NT 胚胎开发了小狗,而那些为为实时出生是必要的 reprogramming 从迟了的经过 R1 ES 施主房间失去了潜力。我们随后建立了近来从 NT 胚囊被开发的 sequentialNT-R1-ESC 线经过 R1 转换字符施主。然而,当在他们的早段落用作原子转移施主时,这些 NT-R1-ESC 排队,没能导致实时小狗。这显示用顺序的 NT 转换字符的治疗学的克隆过程不能救居住在以前的施主代的发展缺乏。 | Chunli Zhao Ruqiang Yao Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang | 2007 | Cell Research2007，17，1： | 1 |
| 5 | SCFβ-TRCP E3 ubiquitin ligase targets the tumor suppressor ZNRF3 for ubiquitination and degradation显示文摘 | Yanpeng Ci Xiaoning Li Maorong Chen Jiateng Zhong Brian J. North Hiroyuki Inuzuka Xi He Yu Li Jianping Guo Xiangpeng Dai | 2018 | Protein & Cell2018，9，10： | 1 |
| 6 | A Hu sheep genome with the first ovine Y chromosome reveal introgression history after sheep domestication显示文摘The Y chromosome plays key roles in male fertility and reflects the evolutionary history of paternal lineages.Here,we present a de novo genome assembly of the Hu sheep with the first draft assembly of ovine Y chromosome(o MSY),using nanopore sequencing and Hi-C technologies.The o MSY that we generated spans 10.6 Mb from which 775 Y-SNPs were identified by applying a large panel of whole genome sequences from worldwide sheep and wild Iranian mouflons.Three major paternal lineages(HY1a,HY1b and HY2)were defined across domestic sheep,of which HY2 was newly detected.Surprisingly,HY2 forms a monophyletic clade with the Iranian mouflons and is highly divergent from both HY1a and HY1b.Demographic analysis of Y chromosomes,mitochondrial and nuclear genomes confirmed that HY2 and the maternal counterpart of lineage C represented a distinct wild mouflon population in Iran that diverge from the direct ancestor of domestic sheep,the wild mouflons in Southeastern Anatolia.Our results suggest that wild Iranian mouflons had introgressed into domestic sheep and thereby introduced this Iranian mouflon specific lineage carrying HY2 to both East Asian and Africa sheep populations. | Ran Li Peng Yang Ming Li Wenwen Fang Xiangpeng Yue Hojjat Asadollahpour Nanaei Shangquan Gan Duo Du Yudong Cai Xuelei Dai Qimeng Yang Chunna Cao Weidong Deng Sangang He Wenrong Li Runlin Ma Mingjun Liu Yu Jiang | 2021 | Science China(Life Sciences)2021，64，7： | 1 |
| 7 | Effects of trace calcium and strontium on microstructure and properties of Cu-Cr alloys显示文摘Cu-0.57Cr-0.01Ca and Cu-0.58Cr-0.01Sr(wt.%)alloys were fabricated and processed by thermo-mechanical treatment.Their mechanical and electrical properties and microstructure were investigated in detail and compared with those of a Cu-0.57Cr(wt.%)alloy.The results showed that the softening resistance of the Cu-Cr alloy was significantly improved by the additions of Ca and Sr elements.Compared with the Cu-Cr alloy,the deformation microstructure of the Cu-Cr-Ca and Cu-Cr-Sr alloys was more difficult to recrystallize at elevated temperatures,and the Cr precipitates in the Cu-Cr-Ca and Cu-Cr-Sr alloys were smaller in size and had an FCC structure at any given aging state.The high strengths of the Cu-Cr-Ca and Cu-Cr-Sr alloys were mainly attributed to the dislocation strengthening provided by high-density dislocations and the precipitate strengthening provided by fine Cr precipitates.First-principles calculation showed that the segregations of Ca and Sr atoms at interface between Cr precipitates and copper matrix were favorable in energetics.This segregation effectively hindered the growth of Cr precipitates and significantly enhanced the pinning effect on the motion of dislocations and subgrain boundaries,eventually leading to the improvement in the softening resistance of the Cu-Cr alloy. | Muzhi Ma Zhu Xiao Xiangpeng Meng Zhou Li Shen Gong Jie Dai Hongyun Jiang Yanbin Jiang Qian Lei Haigen Wei | 2022 | Journal of Materials Science & Technology2022，，17： | 1 |
| 8 | Author Correction:Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle显示文摘The author affiliations were mixed up in the previous published version.In addition,the author contribution section missed a statement of The idea was conceived by J.K.L.,W.W.and This correction does not affect the description of the results or the conclusion of this work.We apologize for any inconvenience this may have caused. | Jing Liuc Yunhua Peng Le Shi Lixin Wanc Hiroyuki Inuzuka Jiangang Long Jianping Guo Jinfang Zhang Min Yuan Shuangxi Zhang Xun Wangc Jing Gao Xiangpeng Dai Shozo Furumoto Lijun Jia Pier Paolo Pandolfi John M.Asara William G.Kaelin Jr. Jiankang Liu Wenyi Wei | 2021 | Cell Research2021，31，1： | 0 |
| 9 | A statistical analysis of conjugate equatorial plasma bubbles based on 630 nm airglow observations显示文摘Using the observations of the 630-nm all-sky imagers(ASIs)located in the geomagnetic conjugate points in the American sector from 2014 to 2017,this study statistically analyzed the features of conjugate equatorial plasma bubbles(EPBs),including their occurrence rate,zonal width,location and zonal drift velocity.The results show that the occurrence rate of the EPBs that occur simultaneously at geomagnetic conjugate points is~84%.The zonal widths of the EPBs are mainly~100 km,and the width differences of EPBs between the northern and southern hemispheres are mainly within±30 km.The zonal displacements of the center locations of the northern and southern EPBs are within±50 km.The zonal drift velocities of the northern and southern EPBs are nearly equal.However,it should be noted that the velocity of the EPBs in the northern hemisphere is 10%faster than that in the southern hemisphere.The results suggest that conjugate EPBs are common.Moreover,the non-conjugate EPBs in the northern and southern hemisphere can occur occasionally,which is probably associated with the different ionospheric backgrounds between the two hemispheres.The features of the conjugate EPBs as shown in this study provides support for the nowcasting of EPBs in the conjugate hemispheres. | Zukang DAI Tao YU Na YANG Jin WANG Di FU Xiangpeng FENG Yutao FENG | 2023 | Science China Earth Sciences2023，66，3： | 0 |
| 10 | Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b显示文摘The epigenetic state of donor cells plays a vital role in the nuclear reprogramming and chromatin remodel-ing of cloned embryos.In this study we investigated the effect of DNA methylation state of donor cells on the development of mouse embryos reconstructed with embryonic stem(ES)cell nuclei.Our results confirmed that deletion of the DNA methyltransferase 3a(Dnmt3a)and DNA methyltransferase 3b(Dnmt3b)distinctly decreases the level of DNA methylation in ES cells.In contrast to wild type ES cells(J1),Dnmt3a–/–3b–/–(DKO)and Dnmt3b–/–(3bKO)donor cells significantly elevated the percentage of embryonic stem cell nuclear transfer(ECNT)morula,blastocysts and postimplantation embryos(P<0.05).However,the efficiency of establish-ment of NT-ES cell lines derived from DKO reconstructed blastocysts was not improved,and the expression pattern of OCT4 and CDX2 in cloned blastocysts and postim-plantation embryos was not altered either.Our results suggest that the DNA methylation state of the donor nucleus is an important factor in regulation of the donor nuclear reprogramming. | Xiangpeng DAI Xiaoyang ZHAO Hai TANG Jie HAO Jean-Paul RENARD Qi ZHOU Alice JOUNEAU Liu WANG | 2010 | Frontiers in Biology2010，5，5： | 0 |
| 11 | Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts显示文摘Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi-or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer- ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning. | FAN Yong TONG Man ZHAO ChunLi DING ChenHui HAO Jie LV Zhuo DAI XiangPeng HAI Tang LI XueMei YAO RuQiang YU Yang LI ZanDong WANG Liu ALICE Jouneau ZHOU Qi | 2008 | Chinese Science Bulletin2008，53，23： | 0 |
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