|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives显示文摘Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades,emphasizing their importance in various assisted reproductive technologies.Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization.Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos,including preservation of the reproductive potential of patients who may become infertile,establishment of cryopreserved oocyte banks,and transport of oocytes and embryos internationally.However,the success rates are still far lower than those obtained with fresh oocytes and embryos,and there are still obstacles that need to be overcome.In this review,we address the major obstacles in the development of effective cryopreservation techniques.Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement.Furthermore,this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. | MOUSSA Mahmoud SHU Juan ZHANG XueHong ZENG FanYi | 2014 | Science China(Life Sciences)2014，57，9： | 18 |
| 2 | Efficient and rapid generation of induced pluripotent stem :ells using an alternative culture medium显示文摘 | Xiao-yang Zhao Wei Li Zhuo Lv Lei Liu Man Tong Tang Hai Jie Hao Chang-long Guo Xia ng Wang Liu Wang Fanyi Zeng Qi Zhou | 2010 | Cell Research2010，20，3： | 9 |
| 3 | Effects of donor cells on in vitro development of cloned bovine embryos显示文摘The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer (SCNT). The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The oocytes were obtained through ovum pick up operation. After in vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer. The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastocyst as the parameters of SCNT efficiency. The donor cells from different individuals (04036, 06081, 06088, and 06129) had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate (P<0.05), and the rate was 62.3%, 37.0%, 35.1%, and 15.6%, respectively. There was no significant difference among the rate of fusion, cleaved and blastocyst in donor cells with different foreign genes (P>0.05). It was concluded that the genetic background of the donor cells could affect the effi- ciency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency. | Jing Fu Pengfei Guan Leiwen Zhao Hua Li Shuzhen Huang Fanyi Zeng Yitao Zeng | 2008 | Journal of Genetics and Genomics2008，35，5： | 6 |
| 4 | Integration-free Methods for Generating Induced Pluripotent Stem Cells显示文摘Induced pluripotent stem(iPS)cells can be generated from mouse or human fbroblasts by exogenous expression of four factors,Oct4,Sox2,Klf4 and c-Myc,and hold great potential for transplantation therapies and regenerative medicine.However,use of retroviral vectors during iPS cell generation has limited the technique’s clinical application due to the potential risks resulting from genome integration of transgenes,including insertional mutations and altered differentiation potentials of the target cells,which may lead to pathologies such as tumorigenesis.Here we review recent progress in generating safer transgene-free or integration-free iPS cells,including the use of non-integrating vectors,excision of vectors after integration,DNA-free delivery of factors and chemical induction of pluripotency. | Yi-ye Zhou Fanyi Zeng | 2013 | Genomics, Proteomics & Bioinformatics2013，11，5： | 3 |
| 5 | Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning显示文摘重建的胚胎从 intersubspecies 导出体的房间原子转移(SCNT ) 从 intrasubspecies SCNT 比那些有更差的发展潜力。把那家荷兰的一种乳牛奶店基于我们的以前的学习牛( HD )在施主 karyoplast 和接受者胞质之间的 mitochondrial DNA ( mtDNA ) haplotype 相容性为 SCNT 胚胎开发是关键的,我们根据聚合酶链反应限制决定的 mtDNA haplotypes 作为接受者胞质作为施主 karyoplast 和 Luxi 黄小母牛( LY )用 HD 执行了 intersubspecies SCNT 碎片长度多型性( PCR-RFLP )分析。结果证明 intersubspecies mtDNA homotype SCNT 胚胎象改进胚囊 reprogramming 地位一样比 intrasubspecies mtDNA heterotype 胚胎有更高的培植前和培植以后的发展胜任,包括象 Oct4 和 Sox2 那样的 pluripotent 基因的正常 H3K9 dimethylation 模式和倡导者 hypomethylation ,用 LY 卵母细胞建议那 intersubspecies SCNT 维持克隆效率的 HD 并且可以发展成正常的改编 HD 原子核克隆动物 ulti 我们的结果显示 karyoplastcytoplast 相互作用和 mtDNA haplotype 相容性可以影响牛的 intersubspecies SCNT 效率。牛的 intersubspecies SCNT 上的这研究为理解在影响牛的 SCNT 效率的 karyoplast 和胞质之间的 mtDNA haplotype 相容性的机制是珍贵的,并且为克隆的 HD 提供一个其他、经济的资源。 | Hao Yan Zhonghai Yan Qingwen Ma Fei Jiao Shuzhen Huang Fanyi Zeng Yitao Zeng | 2011 | Journal of Genetics and Genomics2011，38，1： | 3 |
| 6 | Shared Gene Regulation during Human Somatic Cell Reprogramming显示文摘人的导致的 pluripotent 茎(iPS ) 细胞有能力区分进所有体的细胞并且维持无限的自强。因此,他们为许多疾病在基本研究和临床的治疗有大潜力。识别人的体的房间 reprogramming 的潜在地通用的机制,我们在经历 reprogramming 的房间的三种类型学习了基因表示变化。通常在 iPS 房间的正式就职期间调整的 570 基因的集合包括已知的胚胎的茎(ES ) 房间标记和 pluripotency 相关基因。我们也识别了可能与体的房间 reprogramming 有关的新奇基因和生物范畴。例如,一些下面调整的基因被预言 pluripotency microRNA 簇 miR302/367 的目标,和从这些通常认为的目标基因的蛋白质根据我们的网络分析与干细胞 pluripotency 因素 POU5F1 交往。我们的结果识别了候选人基因集合在在体的房间 reprogramming 期间操作的机制上指导研究。 | Xiang Wang Xuesong Chen Huijun Zhang Wenyi Qin Yan Xue Fanyi Zeng | 2012 | Journal of Genetics and Genomics2012，39，12： | 2 |
| 7 | A Profile of Native Integration Sites Used by φC31 Integrase in the Bovine Genome显示文摘Streptomyces 噬菌体 C31 integrase 能高效地在哺乳动物的染色体以内指向忍受 attB transgenes 到内长的假 attP 地点。更好在牛的染色体理解 C31 integrase 的活动, 44 个集成事件的 DNA 序列被分析,并且 32 个假 attP 地点被识别。这些地点的多数分享包含转换重复并且有类似到野类型的 attP 的一个顺序主题地点。Genomic DNA flanking 这些地点典型地包含了重复顺序元素,例如短、长的散布重复元素。这些顺序特征显示 DNA 顺序识别在指导调停 C31 的地点特定的集成起一个重要作用。另外, BF27 集成热点地点在牛的染色体被识别,它说明了 13.6% 所有孤立的集成事件并且印射到 intron 在肝癌症 1 删除了(DLC1 ) 基因。另外,我们发现在牛的染色体的假 attP 地点在人的染色体和那些一样有另外的特征。这研究代表在牛的染色体的假 attP 地点的顺序特征被分析的第一次。我们断定这个地点特定的 integrase 系统为牛的染色体的应用修正有大潜力。 | Lijuan Qu Qingwen Ma Zaiwei Zhou Haiyan Ma Ying Huang Shuzhen Huang Fanyi Zeng Yitao Zeng | 2012 | Journal of Genetics and Genomics2012，39，5： | 1 |
| 8 | Establishment of customized mouse stem cell lines by sequential nuclear transfer显示文摘治疗学的克隆,胚胎的干细胞(转换字符) 由此从原子转移(NT ) 被导出胚胎,可以在再生药的新时代起一个主要作用。Inthis 学习我们建立了四十原子转移 -- 从不同施主房间类型或段落的 NTembryos 被导出的转换字符(NT 转换字符) 线。我们发现 NT 转换字符能够形成胚胎植物或动物身体。另外, NT 转换字符表示了 pluripotency 干细胞标记试管内并且能区分进胚胎的纸巾体内。从施主房间能形成完整的术语的早经过 R1 的 NT 胚胎开发了小狗,而那些为为实时出生是必要的 reprogramming 从迟了的经过 R1 ES 施主房间失去了潜力。我们随后建立了近来从 NT 胚囊被开发的 sequentialNT-R1-ESC 线经过 R1 转换字符施主。然而,当在他们的早段落用作原子转移施主时,这些 NT-R1-ESC 排队,没能导致实时小狗。这显示用顺序的 NT 转换字符的治疗学的克隆过程不能救居住在以前的施主代的发展缺乏。 | Chunli Zhao Ruqiang Yao Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang | 2007 | Cell Research2007，17，1： | 1 |
| 9 | Amelioration of β^(654)-thalassemia in mouse model with the knockdown of aberrantly spliced β-globin mRNA显示文摘Large amounts of aberrantly spliced mRNA from the β654 allele was present in erythroid cells, which might impair the erythropoiesis. A therapeutic strategy for β-thalassemia was explored by knocking down the aberrantly spliced mRNA of β-globin. Lentiviral vector with siRNA fragment targets on the specific portion of β654-globin aberrantly spliced pre-mRNA was constructed. In HeLa β654 cells, the siRNA vector could reduce approximately 60% of aberrantly spliced mRNA, which was assessed by RT-PCR and qRT-PCR. Furthermore, a disease model of β654 thalassemia mice with lentiviral-mediated siRNA was produced by subzonal injection (named Hβi-Hbbth-4/Hbb+ transgenic mice). Our results showed that the hemotological parameters were improved in Hβi-Hbbth-4/Hbb+ transgenic mice. This study provides a potential way for β654-thalassemia therapy by knocking down the aberrantly spliced β-globin mRNA, whilst supporting that the aberrantly spliced β-globin mRNA may aggravate the disease. | Shuyang Xie Wei Li Zhaorui Ren Jingzhi Zhang Xinbin Guo Shu Wang Shuzhen Huang Fanyi Zeng Yi-Tao Zeng | 2008 | Journal of Genetics and Genomics2008，35，10： | 1 |
| 10 | 小鼠GV卵中母源基因的克隆和其中一个基因在植入前胚表达的研究显示文摘目的 克隆和筛选小鼠生发泡完整卵母细胞 (GV卵 )中母源基因 ,并检测其中一个基因在植入前不同发育阶段胚中的表达 ,初步了解该基因在卵母细胞发育成熟及早期胚发育中的作用。 方法 应用抑制性消减杂交技术 (SSH) ,以生发泡完整的卵母细胞为检测子 (tester) ,8 细胞胚为驱赶子 (driver) ,建立GV卵中母源基因的cDNA文库。通过斑点杂交法进一步筛选GV卵中母源基因的cDNA文库 ,对阳性克隆进行序列测定和同源性分析 ;并采用RT PCR方法观察其中 1个阳性克隆的基因在着床前胚的表达。 结果 克隆得到 18个基因的cDNA序列和 8个同源EST序列在GV卵中特异表达 ,包括PeroxiredoxinⅡ基因。RT PCR显示PeroxiredoxinⅡ基因呈阶段性差异表达 ,在GV卵、MⅡ卵、1 细胞胚和 2 细胞胚有表达 ,但从MⅡ卵开始表达下降。而在 4 细胞胚、8 细胞胚、桑椹胚和囊胚中不表达。 结论 PeroxiredoxinⅡ基因是GV卵中母源基因cDNA文库中的一个基因 ,提示该基因在卵母细胞发育成熟和合子基因激活中起一定作用。 | 王世鄂 黄威权 Zeng Fanyi Richard MSchultz | 2004 | 解剖学报2004，35，4： | 1 |
| 11 | Long-term deregulated human hematopoiesis in goats transplanted in utero with BCR-ABL-transduced lin-CD34* cord blood cells显示文摘 | Fanyi Zeng Shu-Zhen Huang Zhi-Juan Gong Mei-Jue Chen Don A Baldwin Wei Hu Hui Qian Jing-bin Yan Juan Wang Yan Ping Xiao Yves Chalandon Ashley Ringrose Zhao-Rui Ren Allen Eaves Connie Eaves Xiaoyan Jiang | 2013 | Cell Research2013，23，6： | 0 |
| 12 | Autologous peripheral hematopoietic stem-cell transplantation in a patient with refractory pemphigus显示文摘The aim of this study is to explore the effective-ness of autologous peripheral hematopoietic stem-cell transplantation in the treatment of refractory pemphigus.A 35-year-old male patient presented with a 4-year history of recurrent bullae on his trunk and extremities.The dia-gnosis of pemphigus was made on the basis of the clinical,histologic and immunofluorescence findings.The patient had shown resistance to conventional therapy with gluco-corticoid and immunosuppressive agents.Two months before admission,he complained of hip joint pain.X-ray and CT scan revealed aseptic necrosis of the femoral head.Stem-cell mobilization was achieved by treatment with cyclophosphamide,granulocyte colony-stimulating factor(G-CSF)and rituximab.Peripheral blood stem cells were collected via leukapheresis and cryopreserved for later use.Immunoablation was accomplished by using cyclophospha-mide(200 mg/kg;divided into 50 mg/kg on days-5,-4,-3,and-2),antithymocyte globulin(ATG;10 mg/kg;divided into 2.5 mg/kg on days-6,-5,-4,and-3),and rituximab(1200 mg/d;divided into 600 mg/d on days 0 and 7).Autologous peripheral hematopoietic stem cell transplanta-tion was followed by reconstitution of the immune system which was monitored by flow cytometry.The glucocorti-coid was withdrawn immediately after transplantation.The pemphigus titer turned negative 6 weeks after transplanta-tion and remained negative.The patient was in complete drug-free remission with no evidence of residual clinical or serological activity of pemphigus during 1 year of follow-up.The patient’s response suggests that autologous peri-pheral hematopoietic stem cell transplantation may be a potential“cure”for refractory pemphigus.However,fur-ther studies are needed to evaluate the risk-benefit ratio of this approach in patients with pemphigus showing resist-ance to conventional therapy. | Ledong SUN Jing SUN Kang ZENG Fanyi MENG Youtao DIAO Dan XU Liang HUANG Jie ZHAO Qifa LIU | 2008 | Frontiers of Medicine2008，2，2： | 0 |
| 13 | Activation of proHGF by St14 induces mouse embryonic stem cell differentiation显示文摘 | Yan, Xiaoshuang Xue, Yan Zhou, Yiye Cheng, Yan Yin, Shang Ma, Qingwen Zeng, Fanyi | 2016 | Protein & Cell2016，7，8： | 0 |
| 14 | Directed hepatic differentiation from embryonic stem cells显示文摘The liver is the largest internal organ in mammals,and is important for the maintenance of normal physiological functions of other tissues and organs.Hepatitis,cirrhosis,liver cancer and other chronic liver diseases are serious threats to human health,and these problems are compounded by a scarcity of liver donors for transplantation therapies.Directed differentiation of embryonic stem cells to liver cells is a promising strategy for obtaining hepatocytes that can be used for cell transplantation.In vitro hepatocyte differentiation of embryonic stem cells requires a profound understanding of normal development during embryonic hepatogenesis.Here we provide a simple description of hepatogenesis in vivo and discuss directed differentiation of embryonic stem cells into hepatocytes in vitro. | Xuesong Chen Fanyi Zeng | 2011 | Protein & Cell2011，2，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员