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| 1 | Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients显示文摘胃的癌症是世界范围的第四很普通的癌症,与死亡的高率和低 5 年的幸存率。迄今为止,有为胃的癌症的有效治疗学的协议的缺乏。最近的研究建议癌症干细胞(CSC ) 为肿瘤开始,侵略,转移,和抵抗负责到 anticancer 治疗。因此,指向胃的 CSC 的治疗是吸引人的。然而,在人的胃的腺癌(GAC ) 的 CSC 没被描述。这里,我们从 GAC 病人在肿瘤纸巾和外部血识别 CSC。从肿瘤纸巾和病人的外部血被孤立的人的 GAC (GCSC ) 的 CSC 带了 CD44 和 CD54 表面标记,当注入了 immunodeficient 老鼠时,高度类似于原来的人的肿瘤的产生肿瘤,在 vitro 区分了进胃的上皮的房间,并且在 vivo 并且在 vitro 自我更新。我们的调查结果建议有效治疗学的协议必须指向 GCSC。从 GAC 病人的循环的 GCSC 的俘获也为为肿瘤转移潜在地负责的一张批评房间人口的鉴定显示出大潜力,并且为早诊断并且胃的癌症的纵的监视提供一个有效协议。 | Tie Chen Kun Yang Jianhua Yu Wentong Meng Dandan Yuan Feng Bi Fang Liu Jie Liu Bing Dai Xinzu Chen Fang Wang Fan Zeng Hong Xu Jiankun Hu Xianming Mo | 2012 | Cell Research2012，22，1： | 50 |
| 2 | Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize显示文摘In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome. | WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu | 2007 | Chinese Science Bulletin2007，52，2： | 35 |
| 3 | 新疆地区维、汉民族冠状动脉粥样硬化性心脏病患者血脂分析(英文)显示文摘目的通过分析维吾尔族和汉族冠状动脉粥样硬化性心脏病与血脂的水平,探讨两民族冠状动脉粥样硬化性心脏病与血脂的关系以及不同民族间的差异。方法对487例冠状动脉粥样硬化性心脏病(维吾尔族245例,汉族242例)和167例正常人(维吾尔族75例,汉族92例)进行了血清总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、三酰甘油(TG)和空腹血糖(FG)的检测。组间年龄和性别均有可比性。结果维吾尔族冠状动脉粥样硬化性心脏病组TC,LDL-C,TG和FG水平较对照组升高(q=10.01,12.69,4.45,6.78;P<0.01),HDL-C较对照组降低(q=6.95,P<0.01)。汉族冠状动脉粥样硬化性心脏病组TC,LDL-C,TG和FG水平较对照组升高(q=10.06,11.89,6.35,5.96;P<0.01),HDL-C较对照组降低(P<0.01)。两民族冠状动脉粥样硬化性心脏病组间和对照组间差别无统计学意义(q=0.35~1.41,P>0.05)。结论血脂紊乱、血糖升高均为冠状动脉粥样硬化性心脏病的危险因素。 | 楚新梅 孟竹 何秉贤 戴文英 Chu Xinmei MENG Zhu He Bingxian Dai Wenying | 2003 | 中国临床康复2003，7，9： | 30 |
| 4 | Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines显示文摘瞄准:识别在 DNA hyper-methylation 之间的关系并且嘘在 hyperme-thylated 的一修正, silenced 肿瘤压制或在人的胃的癌症房间线并且到的基因倡导者阐明 DNA 甲基化的改变是否能影响嘘一修正。方法:我们使用了染色质免疫降水(薄片) 估计地位的试金嘘在 p16 和 mutL 相当或相同的事物的倡导者区域的一乙酰化作用和甲基化 1 (MLH1 ) 在 2 根胃的癌症房间线, SGC-7901 和 MGC-803 的基因。我们使用了 methylation 特定的 PCR (MSP ) 评估 5-Aza-2'-deoxycytidine (5-Aza-dC ) 的效果, trichostatin A (TSA ) 或他们 DNA 甲基化上的联合治疗地位。我们使用了 RT-PCR 决定是否改变嘘在 5-Aza-dC 和 TSA 治疗以后的一修正地位在基因表示被反映。结果:为在二根房间线的 p16 和 MLH1 基因,与 DNA hypermethylation 联系的 silenced 部位被描绘由嘘一 H3-K9 hypoacetylation 和 hypermethylation 并且嘘一 H3-K4 hypomethylation。有 TSA 的治疗导致了中等增加了嘘在没有效果在上的 silenced 部位的一 H3-K9 乙酰化作用嘘基因表示上的一 H3-K9 甲基化和最小的效果。相反,有 5-Aza-dC 的治疗很快减少了嘘在 silenced 部位的一 H3-K9 甲基化并且导致了二基因的复活。有 5-Aza-dC 和 TSA 的联合治疗是在在显示出 DNA hypermethylation 的部位重新激活基因表示的协同的。同样,嘘一 H3-K4 甲基化没在 TSA 处理以后被影响,并且在 5-Aza-dC 处理以后在 silenced 部位中等增加了。结论:在倡导者 CpG 岛的 DNA 的 Hypermethylation 与肿瘤的 transcriptional silencing 有关压制或基因。在倡导者的不同区域的组蛋白 H3-K9 甲基化在胃的癌症房间与每基因的 DNA 甲基化地位学习了相互关联。然而,嘘一 H3-K9 乙酰化作用和 H3-K4 甲基化相反地在胃的癌症房间与每基因的 DNA 甲基化地位相关。DNA 甲基化的改变影响嘘一修正。 | Chun-Feng Meng Xin-Jiang Zhu Guo Peng Dong-Qiu Dai | 2007 | World Journal of Gastroenterology2007，13，46： | 27 |
| 5 | ^(18)F-FDG uptake as a biologic factor predicting outcome in patients with resected non-small-cell lung cancer显示文摘背景 non-small-cell 肺癌症(NSCLC ) 的外科的治疗的结果仍然保持差。在许多病人, NSCLC 的生物行为不跟随一个明确的模式,并且不能精确地在治疗前被预言。正电子排放断层摄影术(宠物) 上的 18F-fluoro-2-deoxy-glucose (18F-FDG ) 举起与 NSCLC 的攻击性被联系。现在的学习在与回顾的分析用经历了完全的切除术和外科手术前的 FDG 宠物的 82 个病人做的 NSCLC.Methods A 预言通过手术对待的病人的结果集中了于 18F-FDG 举起的角色。最大值标准化了举起价值(SUVmax ) ,除了五个 clinicopathological 因素和三 biomolecular 因素,能可能影响幸存,与病人的复发和幸存为可能的协会被作比较,由比例的危险在 multivariate 分析建模的 univariate 分析和艇长的木头等级测试。在 SUVmax 和另外的因素之间的协会也被分析。有 SUVmax 的结果病人超过 11 与不到 11 在 univariate 分析的 SUVmax 有没有疾病的幸存和比病人短的全面幸存(P0.001, P=0.002 ) 。在 multivariate 分析, SUVmax (在 11 的 dichotomized ) 是为肿瘤复发的唯一的重要预言者。TNM 舞台和 SUVmax (在 11 的 dichotomized ) 是为全面幸存的独立预言者。有 p53 overexpression 的 SUVmax 的协会,增殖的房间把肿瘤的索引和 microvascular 密度标记的原子抗原(PCNA ) 在全部组是重要的。宠物上的结论 18F-FDG 举起可以被用来 noninvasively 在 vivo 估计 NSCLC 的生物攻击性,识别能得益于另外的治疗的有差的预后的通过手术对待的病人。 | ZHANG Zhen-jiang CHEN Jing-han MENG Long DU Jia-jun ZHANG Lin LIU Ying DAI Hong-hai | 2007 | Chinese Medical Journal2007，，2： | 17 |
| 6 | Isoliquiritigenin inhibits TGF-β1-induced fibrogenesis through activating autophagy via PI3K/AKT/mTOR pathway in MRC-5 cells显示文摘Isoliquiritigenin(ISL),a natural flavonoid derived from the root of liquorice,has been reported to possess anti-inflammatory and antioxidant activities.Previous studies have found that ISL plays a crucial role in anti-fibrosis of adipose tissue and renal tissue;however,its effect on pulmonary fibrogenesis has not been demonstrated.In this study,we aimed to explore the roles and the underlying mechanisms of ISL in TGF-β1-induced fibrogenesis using human lung fibroblast-derived MRC-5 cells.Cell proliferation and migration were determined by MTT and wound healing assay,respectively.The expression levels of alpha-smooth muscle actin(α-SMA),collagen type I alpha 1(COLIA1)and fibronectin(FN),microtubule-associated protein light chain 3(LC3)and related signaling molecules were detected by quantitative real-time PCR,western blot and immunofluorescence assay,correspondingly.EGFP-LC3 transfection was used for autophagy analysis.The results showed that ISL inhibited the TGF-β1-induced proliferation and migration,and down-regulated the expressions ofα-SMA,COLIA1 and FN.ISL treatment led to up-regulation of LC3 in TGF-β1-treated MRC-5 cells,accompanied by significant decrease in the phosphorylation levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),and mammalian target of rapamycin(mTOR).In addition,the inhibitory effects of ISL on TGF-β1-induced fibrogenic features in MRC-5 cells were enhanced by pretreatment with autophagy activator Rapmycin and PI3K/AKT inhibitor LY294002 and reversed by autophagy inhibitor 3-methyladenine and PI3K/AKT activator IGF-1.Taken together,our results demonstrated that ISL could attenuate the fibrogenesis of TGF-β1-treated MRC-5 cells by activating autophagy via suppressing the PI3K/AKT/mTOR pathway.Therefore,ISL holds a great potential to be developed as a novel therapeutic agent for the treatment of pulmonary fibrosis. | Jinjuan He Hao Peng Meifang Wang Ying Liu Xingrong Guo Bin Wang Longjun Dai Xueqin Cheng Zhongji Meng Leyong Yuan Fenglin Cai Yijun Tang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，8： | 16 |
| 7 | Sirtuin 3 deficiency exacerbates diabetic cardiomyopathy via necroptosis enhancement and NLRP3 activation显示文摘Sirtuin 3(SIRT3)is a potential therapeutic target for cardiovascular,metabolic,and other aging-related diseases.In this study,we investigated the role of SIRT3 in diabetic cardiomyopathy(DCM).Mice were injected with streptozotocin(STZ,60 mg/kg,ip)to induce diabetes mellitus.Our proteomics analysis revealed that SIRT3 expression in the myocardium of diabetic mice was lower than that of control mice,as subsequently confirmed by real-time PCR and Western blotting.To explore the role of SIRT3 in DCM,SIRT3-knockout mice and 129S1/SvImJ wild-type mice were injected with STZ.We found that diabetic mice with SIRT3 deficiency exhibited aggravated cardiac dysfunction,increased lactate dehydrogenase(LDH)level in the serum,decreased adenosine triphosphate(ATP)level in the myocardium,exacerbated myocardial injury,and promoted myocardial reactive oxygen species(ROS)accumulation.Neonatal rat cardiomyocytes were transfected with SIRT3 siRNA,then exposed to high glucose(HG,25.5 mM).We found that downregulation of SIRT3 further increased LDH release,decreased ATP level,suppressed the mitochondrial membrane potential,and elevated oxidative stress in HG-treated cardiomyocytes.SIRT3 deficiency further raised expression of necroptosis-related proteins including receptor-interacting protein kinase 1(RIPK1),RIPK3,and cleaved caspase 3,and upregulated the expression of inflammation-related proteins including NLR family pyrin domain-containing protein 3(NLRP3),caspase 1 p20,and interleukin-1βboth in vitro and in vivo.Collectively,SIRT3 deficiency aggravated hyperglycemia-induced mitochondrial damage,increased ROS accumulation,promoted necroptosis,possibly activated the NLRP3 inflammasome,and ultimately exacerbated DCM in the mice.These results suggest that SIRT3 can be a molecular intervention target for the prevention and treatment of DCM. | Shu Song Yue Ding Guo-liang Dai Yue Zhang Meng-ting Xu Jie-ru Shen Ting-ting Chen Yun Chen Guo-liang Meng | 2021 | Acta Pharmacologica Sinica2021，42，2： | 14 |
| 8 | Serum Uric Acid is Associated with the Predicted Risk of Prevalent Cardiovascular Disease in a Community-dwelling Population without Diabetes显示文摘Objective To examine the association between serum uric acid levels and cardiovascular disease risk among individuals without diabetes. Methods We investigated the association between serum uric acid levels and the risk of prevalent cardiometabolic diseases, 10-year Framingham risk for coronary heart disease, and 10-year risk for atherosclerotic cardiovascular diseases(ASCVD) among 8,252 participants aged ≥ 40 years without diabetes from Jiading district, Shanghai, China. Results Body mass index, waist circumference, blood glucose, glycated hemoglobin, blood pressure, and serum lipids increased progressively across the sex-specific quartiles of uric acid(all P trend < 0.05). Compared with individuals in the lowest quartile, those in the higher quartiles had a significantly higher prevalence of obesity, hypertension, and dyslipidemia(all P trend < 0.05). A fully adjusted logistic regression analysis revealed that individuals in the highest quartile had an increased risk of predicted cardiovascular disease compared with those in the lowest quartile of uric acid. The multivariate adjusted odds ratios(ORs) [95% confidence intervals(CIs)] for the highest quartiles for high Framingham risk were 3.00(2.00-4.50) in men and 2.95(1.08-8.43) in women. The multivariate adjusted ORs(95% CIs) for the highest quartile for high ASCVD risk were 1.93(1.17-3.17) in men and 4.53(2.57-7.98) in women. Conclusion Serum uric acid level is associated with an increased risk of prevalent obesity, hypertension, dyslipidemia, 10-year Framingham risk for coronary heart disease, and 10-year risk for ASCVD among Chinese adults without diabetes. | CHENG Di DU Rui WU Xue Yan LIN Lin PENG Kui MA Li Na XU Yu XU Min CHEN Yu Hong BI Yu Fang WANG Wei Qing DAI Meng LU Jie Li | 2018 | Biomedical and Environmental Sciences2018，31，2： | 13 |
| 9 | PCA-based sea-ice image fusion of optical data by HIS transform and SAR data by wavelet transform显示文摘Sea ice as a disaster has recently attracted a great deal of attention in China. Its monitoring has become a routine task for the maritime sector. Remote sensing, which depends mainly on SAR and optical sensors, has become the primary means for sea-ice research. Optical images contain abundant sea-ice multi-spectral information, whereas SAR images contain rich sea-ice texture information. If the characteristic advantages of SAR and optical images could be combined for sea-ice study, the ability of sea-ice monitoring would be improved. In this study, in accordance with the characteristics of sea-ice SAR and optical images, the transformation and fusion methods for these images were chosen. Also, a fusion method of optical and SAR images was proposed in order to improve sea-ice identification. Texture information can play an important role in sea-ice classification. Haar wavelet transformation was found to be suitable for the sea-ice SAR images, and the texture information of the sea-ice SAR image from Advanced Synthetic Aperture Radar(ASAR) loaded on ENVISAT was documented. The results of our studies showed that, the optical images in the hue-intensity-saturation(HIS) space could reflect the spectral characteristics of the sea-ice types more efficiently than in the red-green-blue(RGB) space, and the optical image from the China-Brazil Earth Resources Satellite(CBERS-02B) was transferred from the RGB space to the HIS space. The principal component analysis(PCA) method could potentially contain the maximum information of the sea-ice images by fusing the HIS and texture images. The fusion image was obtained by a PCA method, which included the advantages of both the sea-ice SAR image and the optical image. To validate the fusion method, three methods were used to evaluate the fused image, i.e., objective, subjective, and comprehensive evaluations. It was concluded that the fusion method proposed could improve the ability of image interpretation and sea-ice identification. | LIU Meijie DAI Yongshou ZHANG Jie ZHANG Xi MENG Junmin XIE Qinchuan | 2015 | Acta Oceanologica Sinica2015，34，3： | 11 |
| 10 | Effects of radix curcumae-derived diterpenoid C on Helicobacter pylori-induced inflammation and nuclear factor kappa B signal pathways显示文摘AIM:To study effect of diterpenoid C extracted from radix curcumae on Helicobacter pylori(H.pylori)-infected inflammation,intestinal metaplasia,and nuclear factor kappa B(NF-κB)signaling pathway in vitro.METHODS:We used I-type H.pylori to infect human gastric epithelial gastric epithelium cell line(GES-1)cell lines,and then H.pylori-infected GES-1 cells were treated with radix curcumae(RC)-derived diterpenoid C of different concentrations(5,10,20μg/mL)and amoxicillin.The expression of p65,IκB kinase(IKK)αand IKKγproteins was detected with Western blotting,and the expression of interleukin(IL)-8,IL-6 and IL-4 was determined with enzyme-linked immunosorbent assay method.Data were analyzed using SPSS software ver18.0.For comparisons between groups of more than two unpaired values,one-way analysis of variance(ANOVA)was used.If an ANOVA F value was significant,post hoc comparisons were performed between groups.If results were not normally distributed,the Mann-Whitney U test was used to compare two groups of unpaired values,whereas for comparisons between groups of more than two unpaired values,the Kruskal-Wallis H test was used.Statistical significance was established at P<0.05.RESULTS:The MTT assay results revealed the inhibited rate of GES-1,and indicated that the IC5 of RCderived diterpenoid C and amoxicillin all were 5μg/mL for gastric GES-1 cells.The expression of IL-8 was significantly increased,especially at 12 h time point;and the expression of IL-4 was decreased in H.pyloriinfected GES-1 cells.After H.pylori-infected GES-1 cells were treated with RC-derived diterpenoid C of different concentrations and amoxicillin,the expression of IL-8was decreased at 12,24,48,72 h points(P<0.01),especially in high-concentration diterpenoid C(20μg/mL)group;and the expression of IL-4 was increased,especially in moderate and high-concentration diterpenoid C(10 and 20μg/mL)groups.RC-derived diterpenoid C had the inhibitory effects on H.pylori-induced p65 translocation from cytoplasm into cell nucleus,H.pylori-stimulant IkBαdegradation,the phosphorylation of p65 and IkBα,and the expression of IKKαand IKKβproteins.CONCLUSION:RC-derived diterpenoid C can block NF-κB signal pathway,effectively reducing the secretion of H.pylori-induced proinflammatory cytokine and increasing the secretion of anti-inflammatory cytokine. | Xuan Huang Bin Lv Shuo Zhang Qun Dai Bing-Bing Chen Li-Na Meng | 2013 | World Journal of Gastroenterology2013，19，31： | 10 |
| 11 | Chromosome-Level Reference Genome and Population Genomic Analysis Provide Insights into the Evolution and Improvement of Domesticated Mulberry (Morns alba)显示文摘Mulberry(Morns spp.)is the sole plant consumed by the domesticated silkworm.However,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains unclear.Here,we report a high-quality,chromosome-level domesticated mulberry(Morus alba)genome.Analysis of genomic data and karyotype analyses confirmed that M.alba is a diploid with 28 chromosomes(2/7=2x=28).Population genomic analysis based on resequencing of 134 mulberry accessions classified domesticated mulberry into three geographical groups,namely,Taihu Basin of southeastern China(Hu mulberry),northern and southwestern China,and Japan.Hu mulberry had the lowest nucleotide diversity among these accessions and demonstrated obvious signatures of selection associated with environmental adaptation.Further phylogenetic analysis supports a previous proposal that multiple domesticated mulberry accessions previously classified as different species actually belong to one species.This study expands our understanding of genome evolution of the genus Morus and population structure of domesticated mulberry,which would facilitate mulberry breeding and improvement. | Feng Jiao Rongsong Luo Xuelei Dai Hui Liu Gang Yu Shuhua Han Xin Lu Chao Su Qi Chen Qinxia Song Caiting Meng Fanghong Li Hongmei Sun Rui Zhang Tian Hui Yonghua Qian Aichun Zhao Yu Jiang | 2020 | Molecular Plant2020，13，7： | 10 |
| 12 | MTERF1 regulates the oxidative phosphorylation activity and cell proliferation in HeLa cells显示文摘mitochondrial 抄写结束因素(MTERF ) 家庭是一组高度保存的 DNA 有约束力的蛋白质 composedof 四个关键成员, MTERF14。迄今为止,几研究在 mitochondrial genomeand 上调查了 MTERF1 的有约束力的地点 mitochondrial 基因抄写的规定,而是在 mitochondrial 基因 transcriptionregulation 之间的更复杂的连接, mitochondrial 氧化 phosphorylation (OXPHOS ) ,和房间增长仍然糟糕被理解。在这研究,我们构造了在表示上和是进 HeLa 房间的 transfected 调查 MTERF1 的功能的 MTERF1 的击倒的向量。结果证明尽管 MTERF1 是 mitochondrial 基因抄写的一个 positiveregulatory 因素,它没在 MTERF1 的 mitochondrial DNA.Over表示的复制上有重要效果增加的 mitochondrial 氧化 phosphorylation 活动和支持的 ATP 合成, cyclin D1expression ,和房间增长它的击倒的禁止的 ATP 合成,减少的 cyclin D1 表示,并且 slowedthe 房间生长。这些结果建议 MTERF1 可以由在 HeLa 房间调整氧化 phosphorylationactivity 支持房间增长。最终,这些调查结果由提供新奇卓见进位于房间增长下面的潜在的机制在 MTERF 家庭的 physiologicalfunctions 上为进一步、更最后的研究创造一个基础规定。 | Guiyuan Chen Jie Dai Shirui Tan Shengke Meng Zhongjian Liu Meizhang Li Qinghua Cui Min Yu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，6： | 10 |
| 13 | Baihui(DU20)-penetrating-Qubin(GB7) acupuncture inhibits apoptosis in the perihemorrhagic penumbra显示文摘Baihui(DU20)-penetrating-Qubin(GB7) acupuncture can inhibit inflammatory reactions and activate signaling pathways related to proliferation after intracerebral hemorrhage.However,there is no research showing the relationship between this treatment and cell apoptosis.Rat models of intracerebral hemorrhage were established by injecting 60 μL of autologous blood into the right side of the caudate-putamen.Six hours later,the needle traveled subcutaneously from the Baihui acupoint to Qubin acupoint.The needle was alternately rotated(180 ± 10 turns/min) manually along clockwise and counter-clockwise directions.Stimulation lasted for 7 days,and was performed three times each for 6 minutes with 6-minute intervals between stimulations.Rats intraperitoneally receiving Sonic hedgehog pathway activator,purmorphamine(1 mg/kg per day),served as positive controls.Motor and sensory function were assessed using the Ludmila Belayev test.Extent of pathological changes were measured in the perihemorrhagic penumbra using hematoxylin-eosin staining.Apoptosis was examined by terminal deoxynucleotidyl transferase(Td T)-mediated d UTP nick end labeling assay.Expression of smoothened(Smo) and glioma-associated homolog 1(Gli1) was determined by western blot assay.Our results showed that Baihui-penetrating-Qubin acupuncture promoted recovery of motor and sensory function,reduced the apoptotic cell percentage in the perihemorrhagic penumbra,and up-regulated Smo and Gli1 expression.We conclude that Baihui-penetrating-Qubin acupuncture can mitigate hemorrhage and promote functional recovery of the brain in a rat model of intracerebral hemorrhage,possibly by activating the Sonic hedgehog pathway. | Beng Zhang Xiao-Hong Dai Xue-Ping Yu Wei Zou Wei Teng Xiao-Wei Sun Wei-Wei Yu Hao Liu Hui Wang Meng-Juan Sun Meng Li | 2018 | Neural Regeneration Research2018，13，9： | 9 |
| 14 | Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated hepatitis B core antigen enhances hepatitis B virus-specific immune responses in vivo显示文摘肝炎 B 病毒(HBV ) 的活动特定的细胞毒素的 T 淋巴细胞(CTL ) 戏在 HBV 的清理的一个占优势的角色。树枝状的房间(DC ) 是关键介绍抗原的房间并且在有免疫力的回答的开始起一个重要作用。我们以前验证了有效地编码 ubiquitinated 肝炎 B 核心抗原(LV-Ub-HBcAg ) 的那 lentiviral 向量导致成熟的 transduced DC,和成熟 DC 高效地导致了 T 房间极化到 Th1 并且产生了 HBcAg 特定的 CTL 前 vivo。在这研究,在 BALB/c 老鼠的 LV-Ub-HBcAg 的 HBV 特定的有免疫力的回答(H-2K d ) 被评估。LV-Ub-HBcAg 的直接注射增加了 cytokines IL-2 和 IFN- 的生产,这被显示出,得到了强壮的抗体回答,并且显著地产生了 IFN-+ 有在 BALB/c 老鼠的 HBV 特定的 CTL 回答的 CD8 + T 房间。另外, LV-Ub-HBcAg 的直接注射导致了有势力 anti-HBV 免疫者回答,类似于得到由的那些在 vitro-transduced DC。在结论,基于 DC 的治疗学的疫苗的 LV-Ub-HBcAg 得到了特定的抗体免疫者回答并且在 vivo 导致了柔韧的特定的 CTL 活动。 | Shenglan Dai Meng Zhuo Linlin Song Xiaohua Chen Yongsheng Yu Zhenghao Tang Guoqing Zang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 9 |
| 15 | Investigation on a vertical radial flow adsorber designed by a novel parallel connection method显示文摘Due to the increasing global demand for industrial gas, the development of large-scale cryogenic air separation systems has attracted considerable attention in recent years. Increasing the height of the adsorption bed in a vertical radial flow adsorber used in cryogenic air separation systems may efficiently increase the treatment capacity of the air in the adsorber. However, uniformity of the flow distribution of the air inside the adsorber would be deteriorated using the height-increasing method. In order to reduce the non-uniformity of the flow distribution caused by the excessive height of adsorption bed in a vertical radial flow adsorber, a novel parallel connection method is proposed in the present work. The experimental apparatus is designed and constructed; the Computational Fluid Dynamics(CFD) technique is used to develop a CFD-based model, which is used to analyze the flow distribution, the static pressure drop and the radial velocity in the newly designed adsorber. In addition, the geometric parameters of annular flow channels and the adsorption bed thickness of the upper unit in the parallelconnected vertical radial flow adsorber are optimized, so that the upper and lower adsorption units could be penetrated by air simultaneously. Comparisons are made between the height-increasing method and the parallel connection method with the same adsorber height. It is shown that using the parallel connection method could reduce the difference between the maximum and minimum radial static pressure drop by 86.2% and improve the uniformity by 80% compared with those of using the height-increasing method. The optimal thickness ratio of the upper and lower adsorption units is obtained as 0.966, in which case the upper and lower adsorption units could be penetrated by air simultaneously, so that the adsorbents in adsorption space could be used more efficiently. | Zhengshu Dai Meng Yu Daozhe Rui Xuejun Zhang Yang Zhao | 2018 | Chinese Journal of Chemical Engineering2018，26，3： | 9 |
| 16 | A Transgenic Study on Affecting Potato Tuber Yield by Expressing the Rice Sucrose Transporter Genes OsSUT5Z and OsSUT2M显示文摘在许多植物,蔗糖 transporters 为从来源和进口的两个蔗糖出口是必要的进水池,显示功能在吸收划分。为了调查蔗糖 transporters 是否能改进淀粉的收益,种,土豆植物(茄属 tuberosum L。cv。 | Aijun Sun Yan Dai Xinsheng Zhang Chunmin Li Kun Meng Honglin Xu Xiaoli Wei Guifang Xiao Pieter B.F, Ouwerkerk Mei Wang Zhen Zhu | 2011 | Journal of Integrative Plant Biology2011，53，7： | 9 |
| 17 | Association between large artery stenosis,cerebral small vessel disease and risk of ischemic stroke显示文摘We aimed to assess the associations of large artery stenosis(LAS)and cerebral small vessel disease(CSVD)with the risk of ischemic stroke and to investigate their respective and combined contributions.In the prospective population-based Shunyi Study,1,082 stroke-free participants aged 55.9±9.1 years were included.Participants were followed for incident stroke throughout the study period(2013-2019).Total small vessel disease score was used to measure CSVD burden.Cervico-cerebral large artery stenosis was evaluated via brain magnetic resonance angiography and carotid ultrasound.We estimated the risk of ischemic stroke in relation to LAS and CSVD with Cox regression models.During a mean follow-up of 4.2 years,34 participants(3.1%)experienced at least one ischemic stroke.Severe LAS(≥50% stenosis versus no stenosis:HR=3.27(95%CI:1.31-8.18))and high CSVD burden(total small vessel disease score 2-4 versus 0 point:HR=12.73(4.83-33.53))were associated with increased stroke risk independently.In multivariate models,CSVD burden(7.72%)explained a larger portion of the variation in stroke risk than severity of LAS(3.49%).Our findings identified that both LAS and CSVD were associated with future ischemic stroke in asymptomatic subjects,while those with high CSVD burden deserve more attention in primary prevention of stroke. | Fei Han Ding-Ding Zhang Fei-Fei Zhai Jun Xue Jiang-Tao Zhang Shuang Yan Li-Xin Zhou Jun Ni Ming Yao Meng Yang Ming-Li Li Zheng-Yu Jin Qing Dai Shu-Yang Zhang Li-Ying Cui Yi-Cheng Zhu | 2021 | Science China(Life Sciences)2021，64，9： | 9 |
| 18 | The Leu477 and Leu613 of ORF2-Encoded Protein Are Critical in Forming Neutralization Antigenic Epitope of Hepatitis E Virus Genotype 4显示文摘Hepatitis E virus (HEV) genotype 4 was originally identified in China. Its neutralization antigenic epitopes have not been characterized. Recently, we identified a neutralizing monoclonal antibody (mAb) 1G10, which was generated following immunization of mice with p166Chn, a recombinant protein comprising 464-629 amino acids (aa) of the HEV genotype 4 capsid protein. In this study, a panel of 22 N- and/or C-terminal truncated and 6 site-directed mutated p166Chn proteins were prepared. Only those N- or C-terminal truncated proteins containing the region 477-613 aa could react with the mAb 1G10, suggesting the neutralization epitope of HEV genotype 4 is located between aa477 and aa613. However, a both N- and C-terminal truncated protein, pN477-C613, neither reacted to 1G10 nor elicited neutralizing antibodies in mice, while another both terminal truncated protein, pN472-C617, did, suggesting the flanking regions of the pN477-C613 could help to stabilize and allow presentation of the neutralization epitope to the immune system. Substituting Leu477 and/or Leu613 with the polar, uncharged threonine (Thr) caused ≥ 50% reduction of the mutants' immunoreactivity to 1G10, whereas replacement by hydrophobic phenylalanine (Phe) made little impact on the immunoreactivity, revealing functional associations between hydrophobicity of aa at positions 477 and 613 and the antigenicity of p166Chn. These data suggested Leu477 and Leu613 are critical in forming the neutralization epitope of HEV genotype 4. | Hongmei Zhang Xing Dai Xiangnian Shan Jsihong Meng | 2008 | Cellular & Molecular Immunology2008，5，6： | 9 |
| 19 | Clinical and Radiological Profile of Acute Fibrinous and Organizing Pneumonia: A Retrospective Study显示文摘 | Jing-Hong Dai Hui Li Wei Shen Li-Yun Miao Yong-Long Xiao Mei Huang Meng-Shu Cao Yang Wang Bin Zhu Fan-Qing Meng Hou-Rong Cai | 2015 | Chinese Medical Journal2015，，20： | 9 |
| 20 | Fabrication and evaluation of molecularly imprinted regenerated cellulose composite membranes via atom transfer radical polymerization显示文摘A simple and effective method for surface molecularly imprinted composite membranes(MICMs) for artemisinin(Ars) based on regenerated cellulose membranes was first prepared through surfaceinitiated atom transfer radical polymerization(ATRP),and the as-prepared MICMs were then evaluated as adsorbents for selective recognition and separation of Ars molecules.Batch rebinding studies were conducted to determine the specific adsorption equilibrium,kinetics and selective permeation performance.The adsorption capacity of MICMs toward Ars by the Langmuir isotherm model was2.008 mg g^(-1),which was nearly 5.0 times higher than non-molecularly imprinted composite membranes(NICMs).The kinetic property of MICMs was well-fitted by the pseudo-second-order rate equation.The selective permeation experiments were successfully investigated to prove the excellent selective permeation performance for Ars than the competitive analog(artemether). | Yi-Lin Wu Yong-Sheng Yan Jian-Ming Pan Xiao-Hui Dai Wei-Dong Shi Min-Jia Meng | 2014 | Chinese Chemical Letters2014，25，2： | 9 |
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