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| 1 | Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death显示文摘Objective: Squamous esophageal carcinoma is highly prevalent in developing countries, especially in China. Tu Bei Mu (TBM), a traditional folk medicine, has been used to treat esophageal squamous cell carcinoma (ESCC) for a long term. tubeimoside I (TBMS1) is the main component of TBM, exhibiting great anticancer potential. In this study, we investigated the mechanism of TBMS1 cytotoxic effect on EC109 cells. Methods: Comparative nuclear proteomic approach was applied in the current study and we identified several altered protein spots. Further biochemical studies were carried out to detect the mitochondrial membrane potential, cell cycle and corresponding proteins' expression and location. Results: Subcellular proteomic study in the nucleus from EC109 cells revealed that altered proteins were associated with mitochondrial function and cell proliferation. Further biochemical studies showed that TBMS1-induced molecular events were related to mitochondria-induced intrinsic apoptosis and P21-cyclin B1/cdc2 complex-related G2/M cell cycle arrest. Conclusions: Considering the conventional application of TBM in esophageal cancer, TBMS1 therefore may have a great potential as a chemotherapeutic drug candidate for ESCC. | Yang Xu Guanghui Wang Quancheng Chen Ting Lin Zhiping Zeng Qiang Luo Jie Liu Cuiling Sun | 2013 | Chinese Journal of Cancer Research2013，25，3： | 12 |
| 2 | Current perspectives on in vitro maturation and its effects on oocyte genetic and epigenetic profiles显示文摘In vitro maturation(IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years.Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016. Many different aspects of the IVM method may influence oocyte potential,including priming, gonadotrophin, growth factors, and culture times. The culture conditions of IVM result in alterations in the oocyte or cumulus cell transcriptome that are not observed under in vivo culture conditions. Additionally, epigenetic modifications, such as DNA methylation or acetylation, are also different between in vitro and in vivo cultured oocytes. In sum,current IVM technique is still not popular and requires more systematic and intensive research to improve its effects and applications. This review will help point our problems, supply evidence or clues for future improving IVM technique, thus assist patients for fertility treatment or preservation as an additional option. | Cuiling Lu Yaoyao Zhang Xiaoying Zheng Xueling Song Rui Yang Jie Yan Huailiang Feng Jie Qiao | 2018 | Science China(Life Sciences)2018，61，6： | 10 |
| 3 | Granulosa cell proliferation differentiation and its role in follicular development显示文摘Granulosa cells (GCs) are the most important cells in the ovary that undergo serious changes morphologi- cally and physiologically during the processes of follicular proliferation, differentiation, ovulation, lutenization and atresia. Oocyte (OC) directs GC proliferation and differen- tiation, while GCs influence OC maturation. Many ovarian factors are involved in the regulation of these processes via different molecular mechanisms and signal pathways. P38 MAPK can selectively regulate steroidogenesis in GCs con- trolled by FSH; Transcript factors LRH-1 and DAX-1 play an important role in this process; FSH induces GC prolifera- tion and differentiation by stimulating PCNA and StAR ex- pression and steroidogenesis. Activated ERK1/2 signal pathway may be involved in the FSH-regulated GC prolif- eration and differentiation. Therefore,GC is an ideal model for studying cell proliferation, differentiation and interaction as well as signal transduction. This review briefly summa- rizes the latest data in the literature, including the results achieved in our laboratory. | LU Cuiling YANG Wei HU Zhaoyuan LIU Yixun | 2005 | Chinese Science Bulletin2005，50，23： | 3 |
| 4 | Statin therapy improves response to interferon alfa and ribavirin in chronic hepatitis C: A systematic review and meta-analysis显示文摘 | Qianqian Zhu Na Li Qunying Han Pingping Zhang Cuiling Yang Xiaoyan Zeng Yanping Chen Yi Lv Xi Liu Zhengwen Liu | 2013 | Antiviral Research2013，，3： | 3 |
| 5 | Develop-ment and application of a universal TaqMan real-time PCR for quantitation of duck hepatitis B virus DNA显示文摘 | WANG Yawen LI Yiping YANG Cuiling | | 0，，1： | 1 |
| 6 | IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection显示文摘 | Na Li Qianqian Zhu Zhu Li Qunying Han Jinghong Chen Yi Lv Yawen Wang Xiaoyan Zeng Yanping Chen Cuiling Yang Zhengwen Liu | 2013 | Human Immunology2013，，： | 1 |
| 7 | The tumor suppressor SMAD4/DPC4 is essential for epiblast proliferation and mesoderm induction in mice显示文摘 | Yang Xiao Li Cuiling Xu Xiaoling | 1998 | Proc Natl Acad Sci USA1998，95，： | 1 |
| 8 | Genetic variations of PD1 and TIM3 are differentially and interactively associated with the development of cirrhosis and HCC in patients with chronic HBV infection显示文摘 | Zhu Li Na Li Qianqian Zhu Guoyu Zhang Qunying Han Pingping Zhang Meng Xun Yawen Wang Xiaoyan Zeng Cuiling Yang Zhengwen Liu | 2013 | Infection, Genetics and Evolution2013，，： | 1 |
| 9 | Development and application of a universal Taqman real-time PCR for quantitation of duck hepatitis B virus DNA显示文摘 | Yawen Wang Yiping Li Cuiling Yang Lingyun Hui Qunying Han Lieting Ma Quanying Wang Guangxiao Yang Zhengwen Liu | 2013 | Journal of Virological Methods2013，，1： | 1 |
| 10 | Exogenous NO triggers preconditioning via a CGMP-and mito-KATP-dependent mechanism 显示文摘 | Qin Q Yang XM Cuil | 2004 | Am J Physiol Heart Circ Physiol2004，287，2： | 1 |
| 11 | Screening of abiotic stress-responsive cotton genes using a cotton full-length cDNA overexpressing Arabidopsis library显示文摘Cotton(Gossypium hirsutum L.)is a major crop and the main source of natural fiber worldwide.Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,improved stress resistance of this crop plant is urgently needed.In this study,we used Gateway technology to construct a normalized full-length cDNA overexpressing(FOX)library from upland cotton cultivar ZM12 under various stress conditions.The library was transformed into Arabidopsis to produce a cotton-FOX-Arabidopsis library.Screening of this library yielded 6,830 transgenic Arabidopsis lines,of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs.GO and KEGG analyses mapped most of the cotton ESTs to plant biological process,cellular compo-nent,and molecular function categories.Next,156potential stress-responsive cotton genes were identified from the cotton-FOX-Arabidopsis library under drought,salt,ABA,and other stress conditions.Four stress-related genes identified from the library,designated as GhCAS,GhAPX,GhSDH,and GhPOD,were cloned from cotton complementary DNA,and their expression patterns under stress were analyzed.Phenotypic experiments in-dicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress.The method developed in this study lays a foundation for high-throughput cloning and rapid identification of cotton functional genes. | Shengting Li Hao Chen Zhi Hou Yu Li Cuiling Yang Daojie Wang Chun-Peng Song | 2020 | Journal of Integrative Plant Biology2020，62，7： | 0 |
| 12 | FORMULAS FOR INCIPIENT MOTION OF MUD WITH VARIOUS DENSITIES显示文摘FORMULASFORINCIPIENTMOTIONOFMUDWITHVARIOUSDENSITIESYANGMeiqingandWANGCuilingAbstract:Mudformedfromfinecohesivesedimentscanhav... | YANG Meiqing and WANG Cuiling | 1996 | International Journal of Sediment Research1996，11，1： | 0 |
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