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| 1 | Progress in Global Gas Hydrate Development and Production as a New Energy Resource显示文摘Natural gas hydrates have been hailed as a new and promising unconventional alternative energy,especially as fossil fuels approach depletion,energy consumption soars,and fossil fuel prices rise,owing to their extensive distribution,abundance,and high fuel efficiency.Gas hydrate reservoirs are similar to a storage cupboard in the global carbon cycle,containing most of the world's methane and accounting for a third of Earth's mobile organic carbon.We investigated gas hydrate stability zone burial depths from the viewpoint of conditions associated with stable existence of gas hydrates,such as temperature,pressure,and heat flow,based on related data collected by the global drilling programs.Hydrate-related areas are estimated using various biological,geochemical and geophysical tools.Based on a series of previous investigations,we cover the history and status of gas hydrate exploration in the USA,Japan,South Korea,India,Germany,the polar areas,and China.Then,we review the current techniques for hydrate exploration in a global scale.Additionally,we briefly review existing techniques for recovering methane from gas hydrates,including thermal stimulation,depressurization,chemical injection,and CH4-CO2 exchange,as well as corresponding global field trials in Russia,Japan,United States,Canada and China.In particular,unlike diagenetic gas hydrates in coarse sandy sediments in Japan and gravel sediments in the United States and Canada,most gas hydrates in the northem South China Sea are non-diagenetic and exist in fine-grained sediments with a vein-like morphology.Therefore,especially in terms of the offshore production test in gas hydrate reservoirs in the Shenhu area in the north slope of the South China Sea,Chinese scientists have proposed two unprecedented techniques that have been verified during the field trials:solid fluidization and formation fluid extraction.Herein,we introduce the two production techniques,as well as the so-called'fbur-in-one'environmental monitoring system employed during the Shenhu production test.Methane is not currently commercially produced from gas hydrates anywhere in the world;therefore,the objective of field trials is to prove whether existing techniques could be applied as feasible and economic production methods for gas hydrates in deep-water sediments and permafrost zones.Before achieving commercial methane recovery from gas hydrates,it should be necessary to measure the geologic properties of gas hydrate reservoirs to optimize and improve existing production techniques.Herein,we propose horizontal wells,multilateral wells,and cluster wells improved by the vertical and in dividual wells applied during existing field trials.It is noteworthy that relatively pure gas hydrates occur in seafloor mounds,within near-surface sediments,and in gas migration conduits.Their extensive distribution,high saturation,and easy access mean that these types of gas hydrate may attract considerable attention from academia and industry in the future.Herein,we also review the occurrence and development of concentrated shallow hydrate accumulations and briefly introduce exploration and production techniques.In the closing section,we discuss future research needs,key issues,and major challenges related to gas hydrate exploration and production.We believe this review article provides insight on past,present,and future gas hydrate exploration and production to provide guidelines and stimulate new work into the field of gas hydrates. | LIU Liping SUN Zhilei ZHANG Lei WU Nengyou Yichao Qin JIANG Zuzhou GENG Wei CAO Hong ZHANG Xilin ZHAI Bin XU Cuiling SHEN Zhicong JIA Yonggang | 2019 | Acta Geologica Sinica(English Edition)2019，93，3： | 13 |
| 2 | Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death显示文摘Objective: Squamous esophageal carcinoma is highly prevalent in developing countries, especially in China. Tu Bei Mu (TBM), a traditional folk medicine, has been used to treat esophageal squamous cell carcinoma (ESCC) for a long term. tubeimoside I (TBMS1) is the main component of TBM, exhibiting great anticancer potential. In this study, we investigated the mechanism of TBMS1 cytotoxic effect on EC109 cells. Methods: Comparative nuclear proteomic approach was applied in the current study and we identified several altered protein spots. Further biochemical studies were carried out to detect the mitochondrial membrane potential, cell cycle and corresponding proteins' expression and location. Results: Subcellular proteomic study in the nucleus from EC109 cells revealed that altered proteins were associated with mitochondrial function and cell proliferation. Further biochemical studies showed that TBMS1-induced molecular events were related to mitochondria-induced intrinsic apoptosis and P21-cyclin B1/cdc2 complex-related G2/M cell cycle arrest. Conclusions: Considering the conventional application of TBM in esophageal cancer, TBMS1 therefore may have a great potential as a chemotherapeutic drug candidate for ESCC. | Yang Xu Guanghui Wang Quancheng Chen Ting Lin Zhiping Zeng Qiang Luo Jie Liu Cuiling Sun | 2013 | Chinese Journal of Cancer Research2013，25，3： | 12 |
| 3 | Research Progress of Seafloor Pockmarks in Spatio-Temporal Distribution and Classification显示文摘Seafloor pockmarks are important indicators of submarine methane seepages and slope instabilities.In order to promote the understanding of submarine pockmarks and their relationship with sediment instabilities and climate changes,here we summarize the research results of pockmarks in the spatio-temporal distributions and shaping factors.Most of pockmarks occur along active or passive continental margins during the last 25 kyr B.P..Circular and ellipse are the most common forms of pockmarks,whereas pockmarks in a special crescent or elongated shape are indicators of slope instabilities,and ring-shape pockmarks are endemic to the gas hydrate zones.Further researches should be focused on the trigger mechanism of climate changes based on the pockmarks in the high latitudes formed during the deglaciation periods,and the role of gas hydrates in the seafloor evolution should be elucidated.In addition,the feature of pockmarks at their early stage(e.g.,developing gas chimneys and gas driving sedimentary doming)and the relations between pockmarks and mass movements,mud diapirs could be further studied to clarify the influences of rapid methane release from submarine sediments on the atmospheric carbon contents. | XU Cuiling XU Guoqiang XING Junhui SUN Zhilei WU Nengyou | 2020 | Journal of Ocean University of China2020，19，1： | 1 |
| 4 | Graph-Based Dimensionality Reduction for Hyperspectral Imagery: A Review显示文摘Hyperspectral image(HSI)contains a wealth of spectral information,which makes fine classification of ground objects possible.In the meanwhile,overly redundant information in HSI brings many challenges.Specifically,the lack of training samples and the high computational cost are the inevitable obstacles in the design of classifier.In order to solve these problems,dimensionality reduction is usually adopted.Recently,graph-based dimensionality reduction has become a hot topic.In this paper,the graph-based methods for HSI dimensionality reduction are summarized from the following aspects.1)The traditional graph-based methods employ Euclidean distance to explore the local information of samples in spectral feature space.2)The dimensionality-reduction methods based on sparse or collaborative representation regard the sparse or collaborative coefficients as graph weights to effectively reduce reconstruction errors and represent most important information of HSI in the dictionary.3)Improved methods based on sparse or collaborative graph have made great progress by considering global low-rank information,local intra-class information and spatial information.In order to compare typical techniques,three real HSI datasets were used to carry out relevant experiments,and then the experimental results were analysed and discussed.Finally,the future development of this research field is prospected. | Zhen Ye Shihao Shi Zhan Cao Lin Bai Cuiling Li Tao Sun Yongqiang Xi | 2021 | Journal of Beijing Institute of Technology2021，30，2： | 1 |
| 5 | Approaches to studying and perceptions of the academic environment among university students in China显示文摘 | Linda Price JohnT.E. Richardson Bernadette Robinson Xia Ding Xiaoling Sun Cuiling Han | 2011 | Asia Pacific Journal of Education2011，，2： | 1 |
| 6 | Effects of Different Enucleation Methods on Developmental Potency of Pig Handmade Clone Reconstructed Embryos显示文摘Effects of different enucleation methods on developmental potency of pig handmade clone(HMC)reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body(Pb1)positioning method and demecolcine(DM)assisted enucleation,as well as their effects on development of HMC reconstructed embryos.The results showed that overall enucleation efficiency of Pb1 positioning method was significantly higher than that of blind cut method(P<0.05).The protuberance rate and overall enucleation efficiency of 0.4μg/mL DM treated group for 60 min was significantly higher than that of other concentrations and time treatment groups(P<0.05).For effects on development of HMC reconstructed embryos,there was no significant difference between DM-assisted enucleation and Pb1 positioning method.In conclusion,appropriate addition of DM could enhance enucleation efficiency of HMC,which had no significant influence on developmental potency of reconstructed embryos. | Lv Lingyan Lu Xingrong Sun Junming Pan Cuiling Lan Haien Wang Yanling Cui Kuiqing Xie Bingkun | 2016 | Animal Husbandry and Feed Science2016，8，3： | 0 |
| 7 | Back Pressure Characteristics for Turbine Channel of External-Parallel TBCC Inlet显示文摘For investigating the back pressure characteristics of turbine channel of an external-parallel turbine-based combined cycle(TBCC)inlet during the mode transition with the freestream air Mach number of 1.8,wind tunnel tests and numerical simulations are carried out.The results show that the critical back pressure of the turbine channel decreases linearly with the decrease of the open degree of splitter plate.The turbine channel has self-starting capacity when the open degree of the turbine channel is 100%.The total pressure recovery coefficient increases with the increase of back pressure when turbine channel is at supercritical and critical state.The mass capture ratio,total pressure recovery coefficient and outlet pressure ratio decrease obviously when turbine channel is at subcritical state.Results of the research would provide scheme reference and technology storage for TBCC propulsion evolution. | SUN Bo XIANG Cuiling ZHUO Changfei | 2019 | Transactions of Nanjing University of Aeronautics and Astronautics2019，36，5： | 0 |
| 8 | The Physiological and Molecular Responses of Exogenous Selenium to Selenium Content and Fruit Quality in Walnut显示文摘To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast selenium and sodium selenite solutions were applied on the leaf of walnut,and the selenium levels in leaves,pericarp and kernel were determined at the ripening stage.The fruit quality,mineral nutrient content,antioxidant enzyme activity,and related genes’expression were analyzed.The results showed that the three exogenous selenium increased the selenium levels in leaves,pericarp and kernel of walnut.They also significantly increased fruit and kernel weights,and kernel linoleic acid,but markedly decreased kernel crude fat and saturated fatty acid.Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc,but inhibited the absorption of calcium,and had no significant effect on iron and magnesium in the kernel.Three exogenous selenium increased the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)significantly in the kernel.Except for sodium selenate treatment significantly reduced malondialdehyde(MDA)content in the kernel,the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide(H2O2)levels.They also increased the expression of JrCu/Zn-SOD,Jr2-Cys POD,JrCyt-APX and JrCAT in kernel,to different extents.These implies that,in the walnut fruit enlargement period,the foliar spraying selenium could increase the selenium content of walnut,affect the mineral nutrient absorption,improve the antioxidant capacity and related genes’expression,and reduce the degree of peroxide,and then improve the quality of fruit.Furthermore,yeast selenium showed the comprehensive effect of the best. | Mufang Sun Xinran Hui Cuiling Tong Longyi Yuan Dejian Zhang | 2023 | Phyton-International Journal of Experimental Botany2023，92，3： | 0 |
| 9 | Cloning and functional analysis of the promoter of allergen gene Ara h 1 from peanut显示文摘Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites.Seed-specific promoters(SSPs)can direct the expression of genes specifically in seeds to avoid undesirable effects associated with constitutive expression.However,few SSPs have been identified in peanut.Previous studies have shown that some allergen-encoding genes encode seed storage proteins or exhibit seed-specific/preferential expression.In this study,we characterized allergen-encoding genes from across the genomes of Arachis species to explore seed-specific genes.We found that at least 9 out of 16 identified peanut allergen-encoding genes were expressed specifically in the seeds or were preferentially expressed.A 1493-bp promoter fragment of allergen gene Ara h 1(we named it AHSSP6)was isolated from cultivated peanut genome.cis-element analysis showed that three RY repeat elements which usually exsisted in seed or embryo specific promoter sequence were also present in AHSSP6 sequence.Histochemical analysis showed AHSSP6 could drive the expression of aβ-glucuronidase(GUS)reporter gene specifically in the seeds or cotyledon tissue of transgenic Arabidopsis,while not in other tissues.These findings indicated that these promoters of allergen genes were candidate SSPs,and AHSSP6 was a novel SSP which could be potentially utilized in peanut improvement. | Cuiling Yuan Chunjuan Li Caixia Yan Xiaobo Zhao Juan Wang Yifei Mou Zhiwei Wang Quanxi Sun Shihua Shan | 2022 | Oil Crop Science2022，7，1： | 0 |
| 10 | Inductive effect of hydroxyl safflower yellow-A on apoptosis in abnormal HUVEC via the mitochondrial pathway显示文摘Objective:To investigate the mechanism by which hydroxyl safflower yellow A,an active component of safflower (Carthamus tinctorius L.),promotes apoptosis in abnormal human umbilical vein endothelial cells (HUVECs).Methods:Supernatant of BGC-823 was used to stimulate HUVECs to establish a model of abnormal proliferation of HUVECs.After determining an ideal concentration of HSYA by MTT assay,apoptosis was detected with flow cytometry and TUNEL assay.Mechanism of apoptosis was assessed using quantitative real-time polymerase chain reaction,Western blot,and ELISA.Results:A range of concentrations of HSYA inhibited proliferation and promoted apoptosis of abnormal HUVECs.As the rate of apoptosis increased,mRNA expression of caspase-3 increased while expression of mutant p53 decreased.HSYA had no effect on Fas gene expression.Analogously,protein expression of Bax was increased while those of Bcl-2,Fas,and Fas-L were decreased.Conclusions:HSYA appears to induce apoptosis of HUVECs with the stimulation of the supematant of tumor cells.The mechanism of apoptosis by HSYA may involve activation of the mitochondrial apoptotic pathway and regulation of the expressions of Bcl-2,Bax,and p53. | Na Si Jingjing Wang Yingying Xu Lintao Liu Xu Wang Hongmei Sun Zong Lin Xixi Wang Li Liu Qi Zhang Qian Zhang Cuiling Feng | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，1： | 0 |
| 11 | Pore-water geochemistry in methane-seep sediments of the Makran accretionary wedge off Pakistan:Possible link to subsurface methane hydrate显示文摘Cold seeps are pervasive along the continental margin worldwide,and are recognized as hotspots for elemental cycling pathway on Earth.In this study,analyses of pore water geochemical compositions of one-400 cm piston core(S3)and the application of a mass balance model are conducted to assess methane-associated biogeochemical reactions and uncover the relationship of methane in shallow sediment with gas hydrate reservoir at the Makran accretionary wedge off Pakistan.The results revealed that approximately 77%of sulfate is consumed by the predominant biogeochemical process of anaerobic oxidation of methane.However,the estimated sulfate-methane interface depth is-400 cm below sea floor with the methane diffusive flux of 0.039 mol/(m^(2)·a),suggesting the activity of methane seepage.Based on the δ^(13)C_(DIC) mass balance model combined with the contribution proportion of different dissolved inorganic carbon sources,this study calculated the δ^(13)C of the exogenous methane to be-57.9‰,indicating that the exogenous methane may be a mixture source,including thermogenic and biogenic methane.The study of pore water geochemistry at Makran accretionary wedge off Pakistan may have considerable implications for understanding the specific details on the dynamics of methane in cold seeps and provide important evidence for the potential occurrence of subsurface gas hydrate in this area. | Xianrong Zhang Jianming Gong Zhilei Sun Jing Liao Bin Zhai Libo Wang Xilin Zhang Cuiling Xu Wei Geng | 2021 | Acta Oceanologica Sinica2021，40，9： | 0 |
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