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| 1 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
| 2 | Production of a defensin-like antifungal protein NFAP from Neosartorya fischeri in Pichia pastoris and its antifungal activity against filamentous fungal isolates from human infections显示文摘 | Máté Virágh Dóra V?r?s Zoltán Kele Laura Kovács ádám Fizil Gergely Lakatos Gergely Maróti Gyula Batta Csaba Vágv?lgyi László Galgóczy | 2014 | Protein Expression and Purification2014，，： | 1 |
| 3 | Gustatory perception alterations in obesity: An fMRI study显示文摘 | Csaba Szalay Mihály Aradi Attila Schwarcz Gergely Orsi Gábor Perlaki Lívia Németh Sophia Hanna Gábor Takács István Szabó László Bajnok András Vereczkei Tamás Dóczi József Janszky Sámuel Komoly Péter ?rs Horváth László Lénárd Zoltán Karadi | 2012 | Brain Research2012，，： | 1 |
| 4 | Detection of Uric Acid with New Type of Conducting Polymer-Based BnzymaticSensor by Bipotentiostatic Technique显示文摘 | Gabor H Csaba V | 1999 | Anal Chim ACTA1999，385，： | 1 |
| 5 | Building development monitoring in multitemporal remotely sensed image pairs with stochastic birth-death dynamics显示文摘 | Csaba B Xavier D Josiane Z | 2012 | IEEE Transactions on Pattern Analysis and Machine Intelligence2012，34，1： | 1 |
| 6 | Detection of uric with a new type of conducting polyner-based enzymatic sensor by biopotentiostatic technique显示文摘 | Robert D Gabor H Csaba V | 1999 | Analytica Chimica Acta1999，385，： | 1 |
| 7 | Use of Pichia pastoris for Production of Recombinant Cytokines显示文摘 | Kevin P M Csaba P Mark D M | 2001 | Interleukin Protocols2001，60，： | 1 |
| 8 | Late endocarditis of Amplatzer atrial septal occluder device in a child显示文摘Bacterial endocarditis following atrial septal defect closure using Amplatzer device in a child is extremely rare. We report a 10-year-old girl who developed late bacterial endocarditis, 6 years after placement of an Amplatzer atrial septal occluder device. Successful explantation of the device and repair of the resultant septal defect was carried out using a homograft patch. The rare occurrence of this entity prompted us to highlight the importance of a closed long-term follow up, review the management and explore preventive strategies for similar patients who have multiple co-morbidities and a cardiac device. A high index of suspicion is warranted particularly in pediatric patients. | Neerod K Jha Laszlo Kiraly John SK Murala Csaba Tamas Haitham Talo Hazem El Badaoui Magdi Tofeig Malaika Mendonca Sameer Sajwani Mary A Thomas Sura Ahmed Al Doory Mohammad D Khan | 2015 | World Journal of Cardiology2015，7，10： | 1 |
| 9 | Fractal Description of Dendrite Growth during Electrochemical Migration显示文摘 | Csaba D Gabor H | 2008 | Microelectronics Reliability2008，48，0： | 1 |
| 10 | Characterization of packed beds of plant materials processed by supercritical fluid extraction 显示文摘 | BENCE N BELA S CSABA D A | | Journal of Food Engineering0，88，1： | 1 |
| 11 | Association of activated vitamin D treatment and mortality in chronic kidney disease显示文摘 | Csaba P Kovesdy M D Shahram A | 2008 | Arch Intern Med2008，168，4： | 1 |
| 12 | Association of Cumulatively Low or High Serum Calcium Levels with Mortality in Long-Term Hemodialysis Patients显示文摘 | Miller Jessica E Kovesdy Csaba P Norris Keith C Mehrotra Rajnish Nissenson Allen R Kopple Joel D Kalantar-zadeh Kamyar | 2010 | American Journal of Nephrology2010，，5： | 1 |
| 13 | Modulation of heparan sulfate/chondroitin sulfate ratio by glycosaminoglycan biosynthesis inhibitors affects liver metabotic potential of tumor cells显示文摘 | Jozsef T Csaba D Iren B | 1995 | Int J Cancer1995，62，6： | 1 |
| 14 | Coenzyme Q induces nigral mitoehondrial uncoupling and prevents dopamine cell loss in a primate model of parkinson's disease显示文摘 | Tamas LH Sabrina D Csaba L | 2003 | Endocrinology2003，144，7： | 1 |
| 15 | Gradient elution in non-linear preparative liquid chromatography显示文摘 | ANTIA F D CSABA H | 1989 | Journal of Chro-matography A1989，484，： | 1 |
| 16 | Taguchi optimization of ELISA procedures显示文摘 | Csaba J Orsolya D Anna L | 1999 | J Immunol Meth1999，223，： | 1 |
| 17 | Integrative phosphoproteomics defines two biologically distinct groups of KMT2A rearranged acute myeloid leukaemia with different drug response phenotypes显示文摘Acute myeloid leukaemia(AML)patients harbouring certain chromosome abnormalities have particularly adverse prognosis.For these patients,targeted therapies have not yet made a significant clinical impact.To understand the molecular landscape of poor prognosis AML we profiled 74 patients from two different centres(in UK and Finland)at the proteomic,phosphoproteomic and drug response phenotypic levels.These data were complemented with transcriptomics analysis for 39 cases.Data integration highlighted a phosphoproteomics signature that define two biologically distinct groups of KMT2A rearranged leukaemia,which we term MLLGA and MLLGB.MLLGA presented increased DOT1L phosphorylation,HOXA gene expression,CDK1 activity and phosphorylation of proteins involved in RNA metabolism,replication and DNA damage when compared to MLLGB and no KMT2A rearranged samples.MLLGA was particularly sensitive to 15 compounds including genotoxic drugs and inhibitors of mitotic kinases and inosine-5-monosphosphate dehydrogenase(IMPDH)relative to other cases.Intermediate-risk KMT2A-MLLT3 cases were mainly represented in a third group closer to MLLGA than to MLLGB.The expression of IMPDH2 and multiple nucleolar proteins was higher in MLLGA and correlated with the response to IMPDH inhibition in KMT2A rearranged leukaemia,suggesting a role of the nucleolar activity in sensitivity to treatment.In summary,our multilayer molecular profiling of AML with poor prognosis and KMT2A-MLLT3 karyotypes identified a phosphoproteomics signature that defines two biologically and phenotypically distinct groups of KMT2A rearranged leukaemia.These data provide a rationale for the potential development of specific therapies for AML patients characterised by the MLLGA phosphoproteomics signature identified in this study. | Pedro Casado Ana Rio-Machin Juho JMiettinen Findlay Bewicke-Copley Kevin Rouault-Pierre Szilvia Krizsan Alun Parsons Vinothini Rajeeve Farideh Miraki-Moud David CTaussig Csaba Bödör John Gribben Caroline Heckman Jude Fitzgibbon Pedro R.Cutillas | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 0 |
| 18 | Restoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury显示文摘Efficient in vivo delivery of anti-inflammatory proteins to modulate the microenvironment of an injured spinal cord and promote neuroprotection and functional recovery is a great challenge.Nucleoside-modified messenger RNA(mRNA)has become a promising new modality that can be utilized for the safe and efficient delivery of therapeutic proteins.Here,we used lipid nanoparticle(LNP)-encapsulated human interleukin-10(hIL-10)-encoding nucleoside-modified mRNA to induce neuroprotection and functional recovery following rat spinal cord contusion injury.Intralesional administration of hIL-10 mRNA-LNP to rats led to a remarkable reduction of the microglia/macrophage reaction in the injured spinal segment and induced significant functional recovery compared to controls.Furthermore,hIL-10 mRNA treatment induced increased expression in tissue inhibitor of matrix metalloproteinase 1 and ciliary neurotrophic factor levels in the affected spinal segment indicating a time-delayed secondary effect of IL-105 d after injection.Our results suggest that treatment with nucleoside-modified mRNAs encoding neuroprotective factors is an effective strategy for spinal cord injury repair. | LászlóGál Tamás Bellák Annamária Marton Zoltán Fekécs Drew Weissman Dénes Török Rachana Biju Csaba Vizler Rebeka Kristóf Mitchell B.Beattie Paulo J.C.Lin Norbert Pardi Antal Nógrádi Krisztián Pajer | 2023 | Research2023，，4： | 0 |
| 19 | Capsaicin-sensitive afferentation represents an indifferent defensive pathway from eradication in patients with H.pylori gastritis显示文摘AIM:To study the role of capsaicin-sensitive afferent nerves in Helicobacter pylori (H.pylori) positive chronic gastritis before and after eradication.METHODS:Gastric biopsy samples were obtained from corpus and antrum mucosa of 20 healthy human subjects and 18 patients with H.pylori positive chronic gastritis (n=18) before and after eradication.Tradi-tional gastric mucosal histology (and Warthin-Starry silver impregnation) and special histochemical examina-tions were carried out.Immunohistochemistry for cap-saicin receptor (TRVP1),calcitonin gene-related peptide (CGRP) and substance P (SP) were carried out by the labeled polymer immunohistological method (Lab VisionCo.,USA) using polyclonal rabbit and rat monoclonal antibodies (Abcam Ltd.,UK).RESULTS:Eradication treatment was successful in 16 patients (89%).Seven patients (7/18,39%) re-mained with moderate complaints,meanwhile 11 pa-tients (11/28,61%) had no complaints.At histological evaluation,normal gastric mucosa was detected in 4 patients after eradication treatment (4/18,22%),and moderate chronic gastritis could be seen in 14 (14/18,78%) patients.Positive immuno-staining for capsaicin receptor was seen in 35% (7/20) of controls,89% (16/18,P < 0.001) in patients before and 72% (13/18,P < 0.03) after eradication.CGRP was positive in 40% (8/20) of controls,and in 100% (18/18,P < 0.001) of patients before and in 100% (18/18,P < 0.001) after eradication.The immune-staining of gastric mucosa for substance-P was positive in 25% (5/20) of healthy con-trols,and in 5.5% (3/18,P > 0.05) of patients before and in 0% of patients (0/18,P > 0.05) after H.pylori eradication.CONCLUSION:Distibution of TRVP1 and CGRP is altered during the development of H.pylori positive chronic gastritis.The immune-staining for TRVP1,CGRP and SP rwemained unchanged before and after H.py-lori eradication treatment.The capsaicin-sensitive affer-entation is an independent from the eradication treat-ment.The 6 wk time period might not be enough time for the restituion of chronic H.pylori positive chronic gastritis.The H.pylori infection might not represent the main pathological factor in the development of chronic | Lilla Lakner András Dmtr Csaba Tóth Imre L Szabó gnes Meczker Rebeka Hajós László Kereskai Gyrgy Szekeres Zoltán Dbrnte Gyula Mózsik | 2011 | World Journal of Gastrointestinal Pharmacology and Therapeutics2011，2，5： | 0 |
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