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| 1 | Relevance of α-defensins(HNP1-3) and defensin β-1 in diabetes显示文摘AIM: To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.METHODS: Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients(117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3(human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral leukocytes of the diabetic patients was measured by quantitative RT PCR analysis. Three SNPs of the human DEFB1(human defensin β-1) gene: DEFB1 G-20A(rs11362), DEFB1 C-44G(rs1800972) and DEFB1 G-52A(rs1799946) were genotyped by Custom TaqMan? Real Time PCR assay.RESULTS: Significant differences were observed in HNP1-3 levels between the healthy subjects and both groups of diabetic patients. The mean ± SE was 28.78 ± 4.2 ng/mL in type 1 diabetes, and 29.82 ± 5.36 ng/mL in type 2 diabetes, vs 11.94 ± 2.96 ng/mL in controls; P < 0.01 respectively. There was no significant difference between patients with type 1 and type 2 diabetes in the high plasma concentrations of HNP1-3. The highest concentrations of α-defensin were found in diabetic patients with nephropathy(49.4 ± 4.8 ng/mL), neuropathy(38.7 ± 4.8 ng/mL) or cardiovascular complications(45.6 ± 1.45 ng/L). There was no significant difference in the cumulative copy numbers of DEFA1/DEFA3 genes between controls and patients, or between patients with the two types of diabetes. Comparisons of HNP 1-3 plasma level and DEFA1/A3 copy number of the same patient did not reveal significant relationship between defensin-α levels and the gene copy numbers(r2 = 0.01). Similarly, no positive correlation was observed between the copy numbers and the mRNA expression levels of DEFA1/A3. Regarding the C-44G polymorphism of DEFB1, the GG 'protective' genotype was much less frequent(1%-2%) among both groups of patients than among controls(9%).CONCLUSION: Elevated HNP1-3 levels in diabetes are independent of DEFA1/DEFA3 copy numbers, but GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin β-1 production. | Balázs Csaba Németh Tamás Várkonyi Ferenc Somogyvári Csaba Lengyel Katalin Fehértemplomi Szabolcs Nyiraty Péter Kempler Yvette Mándi | 2014 | World Journal of Gastroenterology2014，20，27： | 4 |
| 2 | Amplification of hormone receptors by neonatal oxytocin and vasopressin treatment显示文摘 | Csaba G Rónai A László V | | 0，，01： | 1 |
| 3 | Production of a defensin-like antifungal protein NFAP from Neosartorya fischeri in Pichia pastoris and its antifungal activity against filamentous fungal isolates from human infections显示文摘 | Máté Virágh Dóra V?r?s Zoltán Kele Laura Kovács ádám Fizil Gergely Lakatos Gergely Maróti Gyula Batta Csaba Vágv?lgyi László Galgóczy | 2014 | Protein Expression and Purification2014，，： | 1 |
| 4 | Aggression to dummy cuckoos by potential European cuckoo hosts显示文摘 | Eivin R?skaft Arne Moksnes B?rd G. Stokke Vítezslav Bicík Csaba Moskát | | Behaviour0，，： | 1 |
| 5 | Controlled domain wall motion in micron-scale permalloy square rings 显示文摘 | Imre A Csaba G Metlushko V | 2003 | PhysicaE2003，19，： | 1 |
| 6 | Apoptosis-inducing factor deficiency induces early mitochondrial degeneration in brain followed by progressive multifocal neuropathology显示文摘 | E1 Ghouzzi V Csaba Z Olivier P | 2007 | J Neuropathol Exp Neurol2007，66，9： | 1 |
| 7 | Detection of Uric Acid with New Type of Conducting Polymer-Based BnzymaticSensor by Bipotentiostatic Technique显示文摘 | Gabor H Csaba V | 1999 | Anal Chim ACTA1999，385，： | 1 |
| 8 | Baker's yeast-mediated synthesis of (R)- and(S)-heteroaryl-ethane-1,2-diols显示文摘 | PAULA V P CSABA P MONICA I T | 2008 | Tetrahedron Asymmetry2008，19，： | 1 |
| 9 | Detection of uric with a new type of conducting polyner-based enzymatic sensor by biopotentiostatic technique显示文摘 | Robert D Gabor H Csaba V | 1999 | Analytica Chimica Acta1999，385，： | 1 |
| 10 | Diabetes mellitus at- tenuates the repolarization reserve in mammalian heart显示文摘 | Csaba L Laszlo V Tamas B | 2007 | Cardiovasc Res2007，73，3： | 1 |
| 11 | Gallbladder hypomotility in diabetic polyneuropathy显示文摘 | Tamás T. Várkonyi M.D. Csaba Lengyel M.D. László Madácsy M.D. Borbála Vel?sy M.D. Ph.D. Péter Kempler M.D. Ph.D. D.Sc. Tamás Fazekas M.D. Ph.D. D.Sc. László Pávics M.D. Ph.D. László Csernay M.D. Ph.D. D.Sc. János Lonovics M.D. Ph.D | 2001 | Clinical Autonomic Research2001，，6： | 1 |
| 12 | Effects of treatment with the plant hormone gibberellin on neonatal rats显示文摘 | Csaba G Darvas S Laszlo V | 1977 | Acta Biol Med Ger1977，36，： | 1 |
| 13 | Aggression to dummy cuckoos by potential European cuckoo hosts显示文摘 | Eivin R?skaft Arne Moksnes B?rd G. Stokke Vítezslav Bicík Csaba Moskát | | 0，，： | 1 |
| 14 | Shedding of the Interleukin-6(IL-6) Receptor (gp80) Determines the Ability of IL-6 to Induce gp130 Phosphorylation in Human Osteoblasts显示文摘 | Csaba V Joshua JJ Jian Z | | 0，，： | 1 |
| 15 | Plasticity of somatostatin and somatostatinsst2A receptors in the rat dentate gyrus during kin- dling epileptogenesis 显示文摘 | Csaba Z Richichi C Bernard V | 2004 | Eur J Neurosci2004，19，： | 1 |
| 16 | On Ekeland vriational principle in b - metric spaces 显示文摘 | Bota M Moinar A Csaba V | 2011 | Fixed Point Theory2011，12，： | 1 |
| 17 | Effects of treatment with the plant hormone gibberellin on neonatal rats显示文摘 | CSABA G DARVAS S LASZL0 V | 1976 | Acta Biologica et Mediea Germaniea1976，36，10： | 1 |
| 18 | Screening in pregnancy with unconjugate serum estriol compared with urinary estriol 显示文摘 | Torok A Csemus V Csaba I | 1988 | Gynecol Obstet Invest1988，26，： | 1 |
| 19 | Diabetes mellitus attenuates the repolarization reserve in mammalian heart显示文摘 | Csaba L LászlóV Tamas B | 2007 | Cardiovaseular Research2007，73，3： | 1 |
| 20 | Genetic variability in the species Rhizopus stolonifer,assessed by random amplified polymorphic DNA analysis显示文摘 | Henrietta Heinrich Klára Acs | 2004 | Antonie van Leeuwenhoek2004，86，： | 1 |
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