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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Air flow obstruction,dyspnea,exercise performance(BODE) index jn chronic obstruction pulmonary diseases显示文摘 | CeUi BR Cote C Marin JM | 2004 | N Engl J Med2004，350，10： | 1 |
| 3 | Support vector network显示文摘 | Cotes C Vapnic V | 1995 | Machine Learning1995，20，1： | 1 |
| 4 | Validation and comparison of reference equations for the 6-rain walk distance test 显示文摘 | Cote CG Casanova C Matin JM | 2008 | Eur Respir J2008，31，3： | 1 |
| 5 | Gasmetal reaction products inthe erosion of chromiumplated gun bores 显示文摘 | COTE P J RICKARD C | 2000 | Wear2000，214，1： | 1 |
| 6 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease 显示文摘 | CELLI B R COTE C G MARIN J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 7 | The body-mass index,airflow obstruction,dyspnea,and exercise capacity index in chronic obstructive pulmonary disease显示文摘 | Celli BR Cote CG Marin JM Casanova C Montes de Oca M Mendez RA Pinto Plata V Cabral HJ | 2004 | N Engl J Med2004，350，10： | 1 |
| 8 | The body mass index, airflow capacity inden in chronic obstructive pulmonary disease显示文摘 | CELLI N M COTE C G MARIN J M | 2004 | N England J Med2004，350，： | 1 |
| 9 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chron- ic obstructive pulmonary disease 显示文摘 | Ce|li B R Cote C G Marin J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 10 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease 显示文摘 | Celli B Cote C G Marin J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 11 | Structure of the chloroplast psbA gene encoding the QB protein from Oryza sativa 显示文摘 | Wu N H Cote C J | 1987 | Develop Genet1987，8，： | 1 |
| 12 | Studies on reac- tivity of tumor and normal cells to a wheat germ agglutinin 显示文摘 | AUB J C SANFORD B H COTE M N | 1965 | Proc Natl Acad Sci USA1965，54，2： | 1 |
| 13 | Corrosion of low carbon steel by microorganisms from the ' pigging' operation debris in water injection pipelines 显示文摘 | COTE C ROSAS O SZTYLER M | 2014 | Bioelectrochemistry2014，97，： | 1 |
| 14 | Temporal Pathogenesis of Experimental Neonatal Woodchuck Hepatitis Virus Infection: Increased Initial Viral Load and Decreased Severity of Acute Hepatitis During the Development of Chronic Viral Infection 显示文摘 | Cote PJ Toshkov I Bellezza C | 2000 | Hepatology2000，32，： | 1 |
| 15 | PCR amplification of microdissected wheat chromosome arms in a simple'single tube'reaction显示文摘 | Albani D Cote M J Armstrong K C | 1993 | Plant J1993，4，5： | 1 |
| 16 | Inspiratory to total lung capacity ratio predictsm ortality in patients with chronic obstructive pulmonary disease显示文摘 | Casanova C Cote C de Torres JP | 2005 | Am J Respir Crit Care Med2005，171，6： | 1 |
| 17 | Evaluation of the quality of prog- nosis studies in systematic reviews显示文摘 | Hayden JA Cote P Bombardier C | 2006 | Ann Intern Med2006，14，6： | 1 |
| 18 | A proteomic analysis of arginine-methylated protein complexes显示文摘 | BOISVERT F M COTE J BOULANGER M C | 2003 | Mol Cell Proteomics2003，2，12： | 1 |
| 19 | Disruption of the nonneu- ronal tphl gene demonstrates the importance of peripheral sero- tonin in cardiac function显示文摘 | Cote F Thevenot E Fligny C | 2003 | Proc Natl Acad Sci USA2003，100，13： | 1 |
| 20 | Analysis of soil wetting and solute transport in subsurface trickle irrigation显示文摘 | COTE C M BRISTOW K L CHARLESWORTH P B | 2003 | Irrigation Science2003，22，34： | 1 |
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