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| 1 | Preprocessing of 2-Dimensional Gel Electrophoresis Images Applied to Proteomic Analysis: A Review显示文摘Various methods and specialized software programs are available for processing twodimensional gel electrophoresis(2-DGE)images.However,due to the anomalies present in these images,a reliable,automated,and highly reproducible system for 2-DGE image analysis has still not been achieved.The most common anomalies found in 2-DGE images include vertical and horizontal streaking,fuzzy spots,and background noise,which greatly complicate computational analysis.In this paper,we review the preprocessing techniques applied to 2-DGE images for noise reduction,intensity normalization,and background correction.We also present a quantitative comparison of non-linear?ltering techniques applied to synthetic gel images,through analyzing the performance of the?lters under speci?c conditions.Synthetic proteins were modeled into a two-dimensional Gaussian distribution with adjustable parameters for changing the size,intensity,and degradation.Three types of noise were added to the images:Gaussian,Rayleigh,and exponential,with signal-to-noise ratios(SNRs)ranging 8–20 decibels(d B).We compared the performanceof wavelet,contourlet,total variation(TV),and wavelet-total variation(WTTV)techniques using parameters SNR and spot ef?ciency.In terms of spot ef?ciency,contourlet and TV were more sensitive to noise than wavelet and WTTV.Wavelet worked the best for images with SNR ranging 10–20 d B,whereas WTTV performed better with high noise levels.Wavelet also presented the best performance with any level of Gaussian noise and low levels(20–14 d B)of Rayleigh and exponential noise in terms of SNR.Finally,the performance of the non-linear?ltering techniques was evaluated using a real 2-DGE image with previously identi?ed proteins marked.Wavelet achieved the best detection rate for the real image. | Manuel Mauricio Goez Maria Constanza Torres-Madronero Sarah Rothlisberger Edilson Delgado-Trejos | 2018 | Genomics, Proteomics & Bioinformatics2018,16,1: | 5 |
| 2 | Management of Helicobacter pylori infection in Latin America: A Delphi technique-based consensus显示文摘AIM: To optimize diagnosis and treatment guidelines for this geographic region, a panel of gastroenterologists, epidemiologists, and basic scientists carried out a structured evaluation of available literature.METHODS: Relevant questions were distributed among the experts, who generated draft statements for consideration by the entire panel. A modified three-round Delphi technique method was used to reach consensus. Critical input was also obtained from representatives of the concerned medical community. The quality of the evidence and level of recommendation supporting each statement was graded according to United States Preventive Services Task Force criteria.RESULTS: A group of ten experts was established. The survey included 15 open-ended questions that were distributed among the experts, who assessed the articles associated with each question. The levels of agreement achieved by the panel were 50% in the first round, 73.3% in the second round and 100% in the third round. Main consensus recommendations included:(1) when available, urea breath and stool antigen test(HpSA) should be used for non-invasive diagnosis;(2) detect and eradicate Helicobacter pylori(H. pylori) in all gastroscopy patients to decrease risk of peptic ulcer disease, prevent o retard progression in patients with preneoplastic lesions, and to prevent recurrence in patients treated for gastric cancer;(3) further investigate implementation issues and health outcomes of H. pylorieradication for primary prevention of gastric cancer in high-risk populations;(4) prescribe standard 14-d triple therapy or sequential therapy for first-line treatment;(5) routinely assess eradication success post-treatment in clinical settings; and(6) select second- and third-line therapies according to antibiotic susceptibility testing.CONCLUSION: These achievable steps toward better region-specific management can be expected to improve clinical health outcomes. | Antonio Rollan Juan Pablo Arab M Constanza Camargo Roberto Candia Paul Harris Catterina Ferreccio Charles S Rabkin Juan Cristóbal Gana Pablo Cortés Rolando Herrero Luisa Durán Apolinaria García Claudio Toledo Alberto Espino Nicole Lustig Alberto Sarfatis Catalina Figueroa Javier Torres Arnoldo Riquelme | 2014 | World Journal of Gastroenterology2014,20,31: | 2 |
| 3 | Capsaicin consumption, Helicobacter pylori CagA status and IL1B-31C <ce:hsp sp='0.25'/>><ce:hsp sp='0.25'/> T genotypes: A host and environment interaction in gastric cancer显示文摘 | Lizbeth López-Carrillo M. Constanza Camargo Barbara G. Schneider Liviu A. Sicinschi Raúl U. Hernández-Ramírez Pelayo Correa Mariano E. Cebrian | 2012 | Food and Chemical Toxicology2012,,6: | 2 |
| 4 | Risk factors for gastric cancer in Latin America: a meta-analysis显示文摘 | Patricia Bonequi Fernando Meneses-González Pelayo Correa Charles S. Rabkin M. Constanza Camargo | 2013 | Cancer Causes & Control2013,,2: | 2 |
| 5 | The value of the world' s eco-system services and natural capital 显示文摘 | Constanza R | 1997 | Nature1997,386,: | 1 |
| 6 | The value of the world's ecosystem services and nature capital显示文摘 | CONSTANZA R DARGER NORTON B | 1997 | Nature1997,387,: | 1 |
| 7 | The value of the world's ecosystem services and natural capital显示文摘 | CONSTANZA R d'ARGE R GROOT R | 1997 | Nature1997,38,6: | 1 |
| 8 | Case–case comparison of smoking and alcohol risk associations with Epstein–Barr virus‐positive gastric cancer显示文摘 | M. Constanza Camargo Chihaya Koriyama Keitaro Matsuo Woo‐Ho Kim Roberto Herrera‐Goepfert Linda M. Liao Jun Yu Gabriel Carrasquilla Joseph J.Y. Sung Isabel Alvarado‐Cabrero Jolanta Lissowska Fernando Meneses‐Gonzalez Yashushi Yatabe Ti Ding Nan Hu Philip R | 2014 | Int J Cancer2014,,4: | 1 |
| 9 | Does the presence of hepatitis virus B and C influence the evolution of diffuse large B-cell lymphoma?显示文摘 | Judit Rubio Fernando Franco Antonio Sá nchez Blanca Cantos Miriam Mé ndez Virginia Calvo Constanza Maximiano David Perez Isabel Millá n Margarita Sá nchez-Beato Mariano Provencio | 2015 | Leukemia & Lymphoma2015,,6: | 1 |
| 10 | The value of the world's ecosystem services and natural capital显示文摘 | CONSTANZA R ARGE R d' RUDOLF de GROOT | 1997 | Nature1997,387,: | 1 |
| 11 | The value of the world' s ecosystem services and natural capital 显示文摘 | Constanza R d'Arge R de Groot R | 1997 | Nature1997,386,6630: | 1 |
| 12 | Global estimates of market and non--market values derived from nighttime satellite imagery,land cover,and ecosystem service valuation显示文摘 | Sutton P C Constanza R | 2002 | Ecological Economies2002,41,: | 1 |
| 13 | The value of the world's ecosystem services and natureal capital显示文摘 | Constanza R Rudolf de Groot el al | 1997 | Nature1997,387,: | 1 |
| 14 | The value of the orld's eeesystem services and natural capital显示文摘 | Constanza R d'Arge R de Groot R | 1997 | Nature1997,387,: | 1 |
| 15 | Thevalue of the worlds ecosystem services and natural capital显示文摘 | Constanza R d Arge R de-Groot R | | 0,,6630: | 1 |
| 16 | The value of the world,s ecosystem services and natural capital 显示文摘 | CONSTANZA R D ARGE R DE G ROOT R | 1997 | Nature1997,,387: | 1 |
| 17 | The value of the world's ecosystem services and natural capital显示文摘 | D'arge R Rudolf DE G | 1997 | Nature1997,387,: | 1 |
| 18 | The value of the world's ecosystem services and natural capital 显示文摘 | Constanza R d' Arge R Rudolf de Groot | 1997 | Nature1997,387,: | 1 |
| 19 | The value of the world ecosystem service andnatural capital显示文摘 | CONSTANZA R GROOT R | 1997 | Nature1997,387,: | 1 |
| 20 | A rapid method for infectivity titration of Andes hantavirus using flow cytometry显示文摘 | Gonzalo P. Barriga Constanza Martínez-Valdebenito Héctor Galeno Marcela Ferrés Pierre-Yves Lozach Nicole D. Tischler | 2013 | Journal of Virological Methods2013,,: | 1 |