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| 1 | TALEN-Mediated Homologous Recombination Produces Site-Directed DNA Base Change and Herbicide-Resistant Rice显示文摘Over the last decades,much endeavor has been made to advance genome editing technology due to its promising role in both basic and synthetic biology.The breakthrough has been made in recent years with the advent of sequence-specific endonucleases,especially zinc finger nucleases(ZFNs),transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPRs) guided nucleases(e.g.,Cas9).In higher eukaryotic organisms,site-directed mutagenesis usually can be achieved through non-homologous end-joining(NHEJ) repair to the DNA double-strand breaks(DSBs) caused by the exogenously applied nucleases.However,site-specific gene replacement or genuine genome editing through homologous recombination(HR) repair to DSBs remains a challenge.As a proof of concept gene replacement through TALEN-based HR in rice(Oryza sativa),we successfully produced double point mutations in rice acetolactate synthase gene(OsALS) and generated herbicide resistant rice lines by using TALENs and donor DNA carrying the desired mutations.After ballistic delivery into rice calli of TALEN construct and donor DNA,nine HR events with different genotypes of OsALS were obtained in T_0 generation at the efficiency of 1.4%—6.3%from three experiments.The HRmediated gene edits were heritable to the progeny of T_1 generation.The edited T_1 plants were as morphologically normal as the control plants while displayed strong herbicide resistance.The results demonstrate the feasibility of TALEN-mediated genome editing in rice and provide useful information for further genome editing by other nuclease-based genome editing platforms. | Ting Li Bo Liu Chih Ying Chen Bing Yang | 2016 | Journal of Genetics and Genomics2016，43，5： | 15 |
| 2 | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol显示文摘Objective:To evaluate the ability of lactic acid bacteria(LAB)strains isolated from fermented mustard to lower the cholesterol in vitro.Methods:The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method.The LAB isolates were analyzed for their resistance to acid and bile salt.Strains with lowering cholesterol activity,were determined adherence to Caco-2 cells.Results:Strain B0007,B0006 and B0022 assimilated more cholesterol than BCRC10474 and BCRC17010.The isolated strains showed tolerance to pH 3.0 for 3h despite variations in the degree of viability and bile-tolerant strains,with more than 10~s CFU/mL after incubation for 24 h at 1%oxigall in MRS.In addition,strain B0007 and B0022 identified as Lactobacillus plantarum with 16S rDNA sequences were able to adhere to the Caco-2 cell lines.Conclusions:These strains B0007 and B0022 may be potential functional sources for cholesterollowering activities as well as adhering to Caco-2 cell lines. | Shu Chen Wang Chen Kai Chang Shu Chang Chan Jiunn Shiuh Shieh Chih Kwang Chiu Pin-Der Duh | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，7： | 10 |
| 3 | 碎裂QRS波联合TIMI危险评分预测急性心肌梗死患者的短期预后(英文)显示文摘目的:探讨碎裂QRS波(fQRS)联合心肌梗死溶栓治疗临床试验(TIMI)危险评分对急性心肌梗死(AMI)患者短期预后的评估价值。创新点:TIMI危险评分常用于AMI患者入院即刻的风险评估,TIMI评分<4分的患者被评定为中危组。本研究发现,无论TIMI评分如何,出现fQRS而未行经皮冠状动脉介入治疗(PCI)的患者,其死亡率均显著升高。fQRS联合TIMI危险评分更利于AMI患者的预后评估,TIMI评分<4分但同时合并fQRS的患者,可以从早期PCI介入治疗获益。方法:回顾300例AMI患者的临床资料及院内诊治经过,记录分析患者fQRS出现的时间、部位及其他临床转归,评价fQRS联合TIMI危险评分与患者临床预后的关系。结论:(1)fQRS联合TIMI危险评分可提高恶性心律失常、左室收缩功能不全(LVSD)和死亡预测的敏感度和特异度;(2)fQRS阳性的AMI患者,进行早期血运重建可降低患者心血管事件发生率,fQRS可能作为TIMI评分<4分患者早期介入治疗的指征。 | Qin-hui SHENG Chih Chi HSU Jian-ping LI Tao HONG Yong HUO | 2018 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2018，19，5： | 8 |
| 4 | High Levels of Hepatitis B Surface Antigen Increase Risk of Hepatocellular Carcinoma in Patients With Low HBV Load显示文摘 | Tai–Chung Tseng Chun–Jen Liu Hung–Chih Yang Tung–Hung Su Chia–Chi Wang Chi–Ling Chen Stephanie Fang–Tzu Kuo Chen–Hua Liu Pei–Jer Chen Ding–Shinn Chen Jia–Horng Kao | 2012 | Gastroenterology2012，，5： | 6 |
| 5 | Effect of platinum on the photocatalytic degradation of chlorinated organic compound显示文摘TiO 2 nanoparticles, doped with different Pt contents, were prepared by a modified photodeposition method using Degussa P-25 TiO 2 , H 2 PtCl 6 ·6H 2 O and methanol as the solvents. The physicochemical properties of Pt/TiO 2 were investigated by the nitrogen adsorption and desorption isotherm measurement technique, X-ray diffraction analysis and photoluminescence spectra, respectively. Reaction rates from photocatalytic removal of dichloromethane over Degussa P-25 TiO 2 and Pt/TiO 2 were evaluated. The average diameter and BET surface area of the TiO 2 catalyst particles were 300 nm and 50 m 2 /g, respectively. The degradation efficiency was 99.0%, 82.7%, 55.2%, and 57.9% with TiO 2 at inlet concentrations of 50, 100, 200, and 300 ppm, respectively. And the degradation efficiency was 99.3%, 79.7%, 76.5%, and 73.4% with a 0.005 wt.% Pt/TiO 2 at inlet concentrations of 50, 100, 200, and 300 ppm, respectively. In addition, we found that the photoluminescence emission peak intensities decreased with increases in the doping amount of Pt, which indicates that the irradiative recombination was weakened. Furthermore, the results showed that the UV/0.005 wt.% Pt/TiO 2 process was capable of efficiently decomposing gaseous DCM in air. | Chih Ming Ma Ya Wen Lee Gui Bing Hong Te Li Su Je Lueng Shie Chang Tang Chang | 2011 | Journal of Environmental Sciences2011，23，4： | 5 |
| 6 | OPTIMUM CHANNEL GEOMETRY AND MINIMUM ENERGY DISSIPATION RATE显示文摘The theory of minimum rate of energy dissipation in conjunction with Yang’s sediment transport equation and Manning’s equation for water discharge is used to derive the optimum open channel geometry. The optimum channel geometry thus derived is consistent with those based on the tractive force approach and is identical to those hosed on the least wetted perimeter for a given cross-sectional area with the maximum conveyance. laboratory and field data are used to support the theoretical analyses. | Chih Ted Yang and Charles C. S. Song International and Special Projects Coordinator, U. S. Bureau of Reclamation, Denyer, Colorado 80225, U. S. A. Professor, St. Anthony Falls Hydraulic Laboratory, University of Minnesota, MinneaPOlis, Minnesota 55414, U. S. A. | 1990 | International Journal of Sediment Research1990，5，1： | 5 |
| 7 | 乳腺浸润性导管癌伴导管原位癌的病理特征及预后关系研究显示文摘乳腺浸润性导管癌(invasive ductal carcinoma, IDC)伴导管原位癌(ductal carcinoma in situ, DCIS)是临床上常见的病理类型。DCIS被普遍认为是IDC的前驱病变,若未经治疗,近一半的DCIS将进展成IDC。IDC伴DCIS的临床病理特征和肿瘤生物学行为可能有别于DCIS和IDC,但IDC中的DCIS成分是否具有预后相关性,目前仍未达成共识。本文阐述了IDC的进展假设模型以及其影响肿瘤生物学的可能因素。此外,基于IDC伴DCIS的临床生物学特性,对IDC肿瘤内的DCIS组织学分级、占比、分子分型和预后方面进行综述。 | Chih Wan Goh 吴佳毅 朱丽 | 2019 | 外科理论与实践2019，24，6： | 5 |
| 8 | 建筑能量循环模式的热力学分析显示文摘以某中学的供暖系统为例,借助CyclePad软件进行仿真建模。用热力学方程组完整地描述了该建筑的两种基本能量循环模式,计算和比较了有新风和无新风、围护结构有保温措施和无保温措施四类模型的系统一次能源利用系数和系统的相对热力学完善程度。 | 王瑞 龚光彩 陈飞虎 Chih Wu | 2009 | 暖通空调2009，39，5： | 4 |
| 9 | APPLICABILITY OF SEDIMENT TRANSPORT FORMULAS显示文摘The paper provides a comprehensive testing of the applicability of 13 sediment transport formulas under different flow and sediment conditions. The dimensionless parameters used for testing the reliability and sensitivity of formulas are dimensionless particle diameter, relative depth, Froude number, relative shear velocity, dimensionless unit stream power, and sediment concentration. A total of 3,391 sets of laboratory and river data are used in the tests. Engineers may find the test results useful to their selection of formulas under different flow and sediment conditions. | Chih Ted YANG and Caian HUANG Manager, Sedimentation and River Hydraulics Group, Technical Service Center, U.S. Bureau of Reclamation, Denver, Colorado 80225. Graduate student, College of Harbour, Waterway and Coastal Engineering, Hohai University, Nanjin | 2001 | International Journal of Sediment Research2001，16，3： | 4 |
| 10 | GSTARS computer models and their applications, Part Ⅱ: Applications显示文摘In part 1 of this two-paper series, a brief summary of the basic concepts and theories used in developing the Generalized Stream Tube model for Alluvial River Simulation (GSTARS) computer models was presented. Part 2 provides examples that illustrate some of the capabilities of the GSTARS models and how they can be applied to solve a wide range of river and reservoir sedimentation problems. Laboratory and field case studies are used and the examples show representative applications of the earlier and of the more recent versions of GSTARS. Some of the more recent capabilities implemented in GSTARS3, one of the latest versions of the series, are also discussed here with more detail. | Francisco J.M.SIM■ES Chih Ted YANG | 2008 | International Journal of Sediment Research2008，23，4： | 4 |
| 11 | Estimating overland flow erosion capacity using unit stream power显示文摘Soil erosion caused by water flow is a complex problem. Both empirical and physically based approaches were used for the estimation of surface erosion rates. Their applications are mainly limited to experimental areas or laboratory studies. The maximum sediment concentration overland flow can carry is not considered in most of the existing surface erosion models. The lack of erosion capacity limitation may cause over estimations of sediment concentration. A correlation analysis is used in this study to determine significant factors that impact surface erosion capacity. The result shows that the unit stream power is the most dominant factor for overland flow erosion which is consistent with experimental data. A bounded regression formula is used to reflect the limits that sediment concentration cannot be less than zero nor greater than a maximum value. The coefficients used in the model are calibrated using published laboratory data. The computed results agree with laboratory data very well. A one dimensional overland flow diffusive wave model is used in conjunction with the developed soil erosion equation to simulate field experimental results. This study concludes that the non-linear regression method using unit stream power as the dominant factor performs well for estimating overland flow erosion capacity. | Hui-Ming SHIH Chih Ted YANG | 2009 | International Journal of Sediment Research2009，24，1： | 4 |
| 12 | Sedation-associated hiccups in adults undergoing gastrointestinal endoscopy and colonoscopy显示文摘AIM:To investigate whether the incidence of hiccups in patients undergoing esophagogastroduodenoscopy (EGD) or same-day bidirectional endoscopy (EGD and colonoscopy;BDE) with sedation is different from those without sedation in terms of quantity,duration and typical onset time.METHODS:Consecutive patients scheduled for elective EGD or same-day BDE at the gastrointestinal endoscopy unit or the health examination center were allocated to two groups:EGD without sedation (Group A) and BDE with sedation (Group B).The use of sedation was based on the patients' request.Anesthesiologists participated in this study by administrating sedative drugs as usual.A single experienced gastroenterologist performed both the EGD and the colonoscopic examinations for all the patients.The incidence,duration and onset time of hiccups were measured in both groups.In addition,the association between clinical variables and hiccups were analyzed.RESULTS:A total of 435 patients were enrolled in the study.The incidences of hiccups in the patients with and without sedation were significantly different (20.5% and 5.1%,respectively).The use of sedation for patients undergoing endoscopy was still significantly associated with an increased risk of hiccups (adjusted odds ratio:8.79,P < 0.001) after adjustment.The incidence of hiccups in males under sedation was high (67.4%).The sedated patients who received 2 mg midazolam developed hiccups more frequently compared to those receiving 1 mg midazolam (P = 0.0028).The patients with the diagnosis of gastroesophageal reflux disease (GERD) were prone to develop hiccups (P = 0.018).CONCLUSION:Male patients undergoing EGD or BDE with sedation are significantly more likely to suffer from hiccups compared to those without sedation.Midazolam was significantly associated with an increased risk of hiccups.Furthermore,patients with GERD are prone to develop hiccups. | Chien Cheng Liu Cheng Yuan Lu Chih Fang Changchien Ping Hsin Liu Daw Shyong Perng | 2012 | World Journal of Gastroenterology2012，18，27： | 4 |
| 13 | Peginterferon Alfa-2a Plus Ribavirin for the Treatment of Dual Chronic Infection With Hepatitis B and C Viruses显示文摘 | Chun–Jen Liu Wan–Long Chuang Chuan–Mo Lee Ming–Lung Yu Sheng–Nan Lu Shun–Sheng Wu Li–Ying Liao Chi–Ling Chen Hsing–Tao Kuo You–Chen Chao Shui–Yi Tung Sien–Sing Yang Jia–Horng Kao Chen–Hua Liu Wei–Wen Su Chih–Lin Lin Yung–Ming Jeng Pei–Jer Chen Ding–Shinn | 2009 | Gastroenterology2009，，2： | 3 |
| 14 | Characterization of proteolipid protein-peptide-specific CD_4^+ T cell of experimental allergic encephalomyelitis in Biozzi AB/H mice | Yong Peng and Chih pin Liu Department of Neurology, The Third Affiliated Hospital, Hunan Medical University , Changsha 410013, China (Peng Y) Division of Immunology, Beckman Research Institute, City of Hope National M edical Center, Duarte, CA 9 | 2002 | Chinese Medical Journal2002，，4： | 3 |
| 15 | Ten‐Day Quadruple Therapy Comprising Proton‐Pump Inhibitor, Bismuth, Tetracycline, and Levofloxacin Achieves a High Eradication Rate for H elicobacter pylori Infection after Failure of Sequential Therapy显示文摘 | Ping‐I Hsu Wen‐Chi Chen Feng‐Woei Tsay Chih‐An Shih Sung‐Shuo Kao Huay‐Min Wang Hsien‐Chung Yu Kwok‐Hung Lai Hui‐Hwa Tseng Nan‐Jing Peng Angela Chen Chao‐Hung Kuo Deng‐Chyang Wu | 2014 | Helicobacter2014，，1： | 3 |
| 16 | Evaluation of total load sediment transport formulas using ANN显示文摘The calculated results from various sediment transport formulas often differ from each other and from measured data. Some parameters in the sediment transport formulas are more effective than others to estimate total sediment load. In this study, an Artificial Neural Network (ANN) model is trained using four dominant parameters of sediment transport formulas. ANN models are able to reveal hidden laws of natural phenomena such as sediment transport process. The results of ANN and some total bed material load sediment transport formulas have been compared to indicate the importance of variables which can be used in developing sediment transport formulas. To train ANN, average flow velocity, water surface slopes, average flow depth, and median particle diameter are used as dominant parameters to estimate total bed material load. Two hundreds and fifty samples are used to train the ANN model. Twenty-four sets of field data not used in the training nor calibration of ANN are used to compare or verify the accuracy of ANN and some well-known total bed material load formulas. The test results show that the ANN model developed in this study using minimum number of dominant factors is a reliable and uncomplicated method to predict total sediment transport rate or total bed material load transport rate. Results show that the accuracy of formulas in descending order are those by Yang (1973), Laursen (1958), Engelund and Hansen (1972), Ackers and White (1973), and Toffaleti (1969). These results are similar to those made by ASCE (1982) based on laboratory and field data not used in this paper. Study results also show that the formulas based on physical laws of sediment transport, like those formulas that were developed based on power concept, are more accurate than other formulas for estimating total bed material sediment load in rivers. | Chih Ted YANG Reza MARSOOLI Mohammad Taghi AALAMI | 2009 | International Journal of Sediment Research2009，24，3： | 3 |
| 17 | MODELING OF RIVER MORPHOLOGIC CHANGES显示文摘MODELINGOFRIVERMORPHOLOGICCHANGESCharlesC.S.SONG;YifanZHENG;andChihTedYANG(Professor,St.AnthonyFallsHydraulicLaboratory,Depar... | Charles C. S. SONG Yifan ZHENG and Chih Ted YANG( , St. Anthony Falls Hydraulic Laboratory, Department of Civil Engineering, University of Minnesota, Minneapolis MN 55414, U. S. A.)( Bechtel Corporation, formerly Reseaxch Assistant, St. Anthony Falls H | 1995 | International Journal of Sediment Research1995，10，2： | 2 |
| 18 | GreenLight HPS laser 120‐W versus diode laser 200‐W vaporization of the prostate: Comparative clinical experience显示文摘 | Po HuiChiang Chien HsuChen Chih HsiungKang Yao ChiChuang | 2010 | Lasers Surg Med2010，，7： | 2 |
| 19 | Promoting innovation through the accumulation of intellectual capital, social capital, and entrepreneurial orientation显示文摘 | Wann‐YihWu Man‐LingChang Chih‐WeiChen | 2008 | R&D Management2008，，3： | 2 |
| 20 | Derivation and verification of minimum energy dissipation rate principle of fluid based on minimum entropy production rate principle显示文摘基于非平衡热力学理论,最小的精力驱散率原则能从最小的熵被导出生产率原则。最小的熵生产率原则等价于最小的精力驱散率原则。为了验证液体运动追随者最小精力驱散率原则, RNG k 骚乱模型和 GMO,流动 3D 当模特儿被使用在笔直地矩形的斜槽模仿液体运动。结果证明那个液体运动满足最小的精力驱散率原则。冲积的河的最小的精力驱散率的有效性与地数据被测试了。当一个河系统以一个相对平衡状态时,它的精力驱散率的价值在最小。最小的值取决于用于河系统的限制。由于一条河的动态性质,然而,最小的价值可以变化与对时间和地点各自。统一溪流力量原则的最小的溪流力量和最小是更一般的最小的精力驱散率原则的特殊、简化的版本。 | Guobin Xu Lina Zhao Chih Ted Yang | 2016 | International Journal of Sediment Research2016，31，1： | 2 |
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