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| 1 | Metallothionein:An overview显示文摘Metallothioneins (MTs) were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal levels of MTs since they are involved in metalloregulatory processes that include cell growth and multiplication. In an effort to understand the biology of this intriguing tumor, various biomarkers such as oncogenes, p53 tumor suppressor gene, waf 1 protein, proliferating cell nuclear antigen, telomerase, microsatellite markers and cytogenetic changes have been examined. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is MT. Immunohistochemical detection of MT proteins in cold acetone-fixed paraffin embedded liver sections was performed by the streptavidin-avidin-biotin immuno- peroxidase complex method. | N Thirumoorthy KT Manisenthil Kumar A Shyam Sundar L Panayappan Malay Chatterjee | 2007 | World Journal of Gastroenterology2007，13，7： | 21 |
| 2 | Vasopressors in obstetric anesthesia: A current perspective显示文摘Vasopressors are routinely used to counteract hypotension after neuraxial anesthesia in Obstetrics. The understanding of the mechanism of hypotension and the choice of vasopressor has evolved over the years to a point where phenylephrine has become the preferred vasopressor. Due to the absence of definitive evidence showing absolute clinical benefit of one over the other, especially in emergency and high-risk Cesarean sections, our choice of phenylephrine over the other vasopressors like mephentermine, metaraminol, and ephedrine is guided by indirect evidence on fetalacid-base status. This review article evaluates the present day evidence on the various vasopressors used in obstetric anesthesia today. | Deb Sanjay Nag Devi Prasad Samaddar Abhishek Chatterjee Himanshu Kumar Ankur Dembla | 2015 | World Journal of Clinical Cases2015，3，1： | 15 |
| 3 | Computer vision-based limestone rock-type classification using probabilistic neural network显示文摘Proper quality planning of limestone raw materials is an essential job of maintaining desired feed in cement plant. Rock-type identification is an integrated part of quality planning for limestone mine. In this paper, a computer vision-based rock-type classification algorithm is proposed for fast and reliable identification without human intervention. A laboratory scale vision-based model was developed using probabilistic neural network(PNN) where color histogram features are used as input. The color image histogram-based features that include weighted mean, skewness and kurtosis features are extracted for all three color space red, green, and blue. A total nine features are used as input for the PNN classification model. The smoothing parameter for PNN model is selected judicially to develop an optimal or close to the optimum classification model. The developed PPN is validated using the test data set and results reveal that the proposed vision-based model can perform satisfactorily for classifying limestone rocktypes. Overall the error of mis-classification is below 6%. When compared with other three classification algorithms, it is observed that the proposed method performs substantially better than all three classification algorithms. | Ashok Kumar Patel Snehamoy Chatterjee | 2016 | Geoscience Frontiers2016，7，1： | 13 |
| 4 | Hepatitis viruses and non-Hodgkin's lymphoma: A review显示文摘Non-Hodgkin's lymphoma(NHL) is among the haematological malignancies with high prevalence worldwide, causing estimated 355 900 new cases and 191 400 deaths in 2008. High prevalence of NHL is documented in economically more developed areas while low prevalence is observed in less developed areas of the globe. A wide array of environmental factors have been reported to be either directly involved or in modifying the risk of NHL development. In addition to these factors, a number of infectious agents, chiefly viruses have also been implicated in the development of NHL. This article reviews the available literature to discuss the role of hepatitis viruses in NHL development, possible mechanisms of lymphomagenesis and also identify the areas in which further research is required to better understand this disease. A brief discussion on the clinical aspects such as classification, staging, treatment approaches have also been included in this article. | Sibnarayan Datta Soumya Chatterjee Rudragoud S Policegoudra Hemant K Gogoi Lokendra Singh | 2012 | World Journal of Virology2012，1，6： | 11 |
| 5 | 纳米技术和可持续发展混凝土:独特的新兴组合(英文)显示文摘在混凝土中加入少量的、至少在一个维度内为纳米尺度(<100 nm)的材料,就可能实现某种性能的提升,达到普通材料无法实现的效果。纳米二氧化硅、纳米碳酸钙、纳米碳管和纤维在改进混凝土性能方面受到越来越多的关注。涂料中二氧化钛纳米粒子可以捕获和分解有机和无机污染物,并使外露的混凝土表面实现自清洁。纳米技术和纳米材料可以使钢筋抗腐蚀能力增强。总的来讲,纳米技术能够使混凝土结构的使用周期中实现包括物质和能量消耗的最小化,同时减少建筑物对环境的负面影响,最终实现建筑物使用中的舒适性和友好性。尽管纳米材料和纳米技术在混凝土结构中有较好的前景,但目前对此类新型材料的对混凝土长期性能了解较少,特别是对其毒性方面的了解更少。随着公众对建筑物各方面安全性越来越关注,需要制定相应的毒性测试分析方法,建立更安全的生产工艺,并在混凝土结构中使用更环保的纳米材料。对纳米技术应用于建筑工业中是否能够真正意义上实现可持续发展进行了综述。 | Anjan K CHATTERJEE | 2015 | 硅酸盐学报2015，43，10： | 10 |
| 6 | 槲皮素诱导肿瘤细胞P53非依赖的G2/M周期阻滞和凋亡显示文摘目的研究槲皮素对肿瘤细胞的增殖、周期和凋亡的影响。方法用槲皮素12.5,25,50和100μmol·L^(-1),分别作用于人肝癌HepG2和Hep3B细胞、人乳腺癌MDA-MA-231细胞、人结肠癌HCT116 p53野生型(HCT116 p53WT)和p53敲除型(HCT116 p53KO)细胞12,24和48 h。荧光显微镜观察细胞形态变化;MTT法测定细胞存活率;采用Hoechst33258染色观察细胞核染色质的变化;采用流式细胞术检测细胞周期的变化;Western印迹法检测胱天蛋白酶3和聚腺苷二磷酸核糖聚合酶(PARP)蛋白水平的变化。结果槲皮素对肿瘤细胞增殖的抑制作用显著且呈浓度(rHepG2=0.972,P<0.01;rHep3B=0.959,P<0.01;rMDA-MB-231=0.979,P<0.01;rHCT116p53WT=0.983,P<0.01;rHCT116p53KO=0.980,P<0.01)和时间(rHepG2=0.974,P<0.01;rHep3B=0.956,P<0.01;rMDA-MB-231=0.959,P<0.01;rHCT116p53WT=0.971,P<0.01;rHCT116p53KO=0.988,P<0.01)依赖性,与细胞对照组相比,槲皮素处理组细胞数量明显减少。槲皮素诱导G_2/M周期阻滞(P<0.01);镜下观察见核染色质浓缩和碎裂等典型的凋亡特性(P<0.01)。Western印迹法结果显示,胱天蛋白酶3蛋白表达水平明显降低(P<0.05,P<0.01),活化的胱天蛋白酶3表达水平无显著变化,PARP表达水平明显降低(P<0.01),活化的PARP明显升高(P<0.01);在加入泛胱天蛋白酶抑制剂后,与槲皮素单独作用相比,胱天蛋白酶3和PARP蛋白表达水平升高,活化的PARP表达水平明显降低(P<0.05,P<0.01,P<0.05)。结论槲皮素能通过诱导P53非依赖性的G_2/M细胞周期阻滞和细胞凋亡,从而实现对肿瘤细胞增殖的抑制作用。 | 王梓萱 周静 唐玥 石欢 黄晓薇 邓敬桓 胡晓晓 陈攀红 李忠杰 戴惠淇 冯吉 Sayantan CHATTERJEE 卢国栋 | 2018 | 中国药理学与毒理学杂志2018，32，10： | 9 |
| 7 | Lee discrepancy on asymmetrical factorials with two-and three-levels显示文摘Lee discrepancy has been employed to measure the uniformity of fractional factorials.In this paper,we further study the statistical justification of Lee discrepancy on asymmetrical factorials.We will give an expression of the Lee discrepancy of asymmetrical factorials with two-and three-levels in terms of quadric form,present a connection between Lee discrepancy,orthogonality and minimum moment aberration,and obtain a lower bound of Lee discrepancy of asymmetrical factorials with two-and three-levels. | CHATTERJEE Kashinath QIN Hong ZOU Na | 2012 | Science China Mathematics2012，55，3： | 7 |
| 8 | Isolation and identification of antibacterial components in seed extracts of Argemone mexicana L.(Papaveraceae)显示文摘Objective:The column chromatographic fractions of chloroform(CH1,CH2,CH3) seed extracts of Argemone mexicana(A.mexicana) were screened for antibacterial activity and phytochemical analysis.Methods:CH3 fraction was isolated and identified by column chromatography,thin layer chromatography,spectral data analysis and phytochemical screening were used for analysis. Results:CH3 fraction was significantly active at 4 to 64 mg/mL against Staphylococcus aureus, Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.125 mg/mL.The active fraction(CH3) revealed presence of alkaloid with retention factor value of 0.44.The active antibacterial agent in the most potent fraction (CH3) was isolated and identified as N-demethyloxysanguinarine by thin layer chromatography (TLC) and phytochemical screening.The CH1 and CH2 fractions did not show inhibitory activity. Conclusions:The results support the ethnomedicinal use of seeds of A.mexicana for treatment of bacterial diseases. | Indranil Bhattacharjee Soroj Kumar Chatterjee Goutam Chandra | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，7： | 7 |
| 9 | Recent advances in molecular diagnostics of hepatitis Bvirus显示文摘Hepatitis B virus(HBV)is one of the important global health problems today.Infection with HBV can lead to a variety of clinical manifestations including severe hepatic complications like liver cirrhosis and hepatocellular carcinoma.Presently,routine HBV screening and diagnosis is primarily based on the immuno-detection of HBV surface antigen(HBsAg).However,identification of HBV DNA positive cases,who do not have detectable HBsAg has greatly encouraged the use of nucleic acid amplification based assays,that are highly sensitive,specific and are to some extent tolerant to sequence variation.In the last few years,the field of HBV molecular diagnostics has evolved rapidly with advancements in the molecular biology tools,such as polymerase chain reaction(PCR)and real-time PCR.Recently,apart of PCR based amplification methods,a number of isothermal amplification assays,such as loop mediated isothermal amplification,transcription mediated amplification,ligase chain reaction,and rolling circle amplification have been utilized for HBV diag-nosis.These assays also offer options for real time detection and integration into biosensing devices.In this manuscript,we review the molecular technologies that are presently available for HBV diagnostics,with special emphasis on isothermal amplification based technologies.We have also included the recent trends in the development of biosensors and use of next generation sequencing technologies for HBV. | Sibnarayan Datta Soumya Chatterjee Vijay Veer Molecular Virology Laboratory | 2014 | World Journal of Gastroenterology2014，20，40： | 6 |
| 10 | Molecular Biology of the Hepatitis B Virus for Clinicians显示文摘 | Sibnarayan Datta Soumya Chatterjee Vijay Veer Runu Chakravarty | 2012 | Journal of Clinical and Experimental Hepatology2012，，4： | 5 |
| 11 | Next-generation sequencing in clinical virology: Discovery of new viruses显示文摘Viruses are a cause of significant health problem world-wide, especially in the developing nations. Due to different anthropological activities, human populations are exposed to different viral pathogens, many of which emerge as outbreaks. In such situations, discovery of novel viruses is utmost important for deciding prevention and treatment strategies. Since last century, a number of different virus discovery methods, based on cell culture inoculation, sequence-independent PCR have been used for identification of a variety of viruses. However, the recent emergence and commercial availability of nextgeneration sequencers(NGS) has entirely changed the field of virus discovery. These massively parallel sequencing platforms can sequence a mixture of genetic materials from a very heterogeneous mix, with high sensitivity. Moreover, these platforms work in a sequenceindependent manner, making them ideal tools for virus discovery. However, for their application in clinics, sample preparation or enrichment is necessary to detect low abundance virus populations. A number of techniques have also been developed for enrichment or viral nucleic acids. In this manuscript, we review the evolution of sequencing; NGS technologies available today as well as widely used virus enrichment technologies. We also discuss the challenges associated with their applications in the clinical virus discovery. | Sibnarayan Datta Raghvendra Budhauliya Bidisha Das Soumya Chatterjee Vanlalhmuaka Vijay Veer | 2015 | World Journal of Virology2015，4，3： | 5 |
| 12 | 健康领域的文化能力显示文摘1背景
尽管人类对其他社会的兴趣纵贯历史的各个阶段,但是检验不同文化概念对人类健康实践的影响从20世纪才开始,当时是伴随着长期的人类学田野调查产生的,这些田野调查揭露了不同文化之间相关卫生实践的多样性、复杂性和连续性。 | Napier A D Ancarno C Butler B Calabrese j Chater A Chatterjee H Guesnet F Home R Jacyna S Jadhav S Macdonald A Neuendorf U Parkhurst A Reynolds R Scambler G Shamdasani S Smith S Z Stougaard-Nielsen J Thomson L Tyler N Volkmann A M Walker T Watson J Williams AC Willott C Wilson J Woolf K | 2016 | 中国卫生政策研究2016，9，2： | 5 |
| 13 | Carbon Dioxide and Nitrous Oxide Emissions from Naturally Occurring Sulfate-Based Saline Soils at Different Moisture Contents显示文摘Soil salinization may negatively affect microbial processes related to carbon dioxide(CO_2) and nitrous oxide(N_2O) emissions. A short-term laboratory incubation experiment was conducted to investigate the effects of soil electrical conductivity(EC) and moisture content on CO_2 and N_2O emissions from sulfate-based natural saline soils. Three separate 100-m long transects were established along the salinity gradient on a salt-affected agricultural field at Mooreton, North Dakota, USA. Surface soils were collected from four equally spaced sampling positions within each transect, at the depths of 0–15 and 15–30 cm. In the laboratory, artificial soil cores were formed combining soils from both the depths in each transect, and incubated at 60% and 90% water-filled pore space(WFPS) at 25?C. The measured depth-weighted EC of the saturated paste extract(EC_e) across the sampling positions ranged from 0.43 to 4.65 dS m^(-1). Potential nitrogen(N) mineralization rate and CO_2 emissions decreased with increasing soil EC_e, but the relative decline in soil CO_2 emissions with increasing ECe was smaller at 60% WFPS than at 90% WFPS. At 60% WFPS, soil N_2O emissions decreased from 133 μg N_2O-N kg^(-1) soil at EC_e < 0.50 dS m^(-1) to 72 μg N_2O-N kg^(-1) soil at EC_e = 4.65 dS m^(-1). In contrast, at 90% WFPS,soil N_2O emissions increased from 262 μg N_2O-N kg^(-1) soil at EC_e = 0.81 dS m^(-1) to 849 μg N_2O-N kg^(-1) soil at EC_e = 4.65 dS m^(-1), suggesting that N_2O emissions were linked to both soil ECe and moisture content. Therefore, spatial variability in soil EC_e and pattern of rainfall over the season need to be considered when up-scaling N_2O and CO_2 emissions from field to landscape scales. | Resham THAPA Amitava CHATTERJEE Abbey WICK Kirsten BUTCHER | 2017 | Pedosphere2017，27，5： | 5 |
| 14 | Comparative analysis of APACHE-Ⅱ and P-POSSUM scoring systems in predicting postoperative mortality in patients undergoing emergency laparotomy显示文摘BACKGROUND Laparotomy remains one of the commonest emergency surgical procedures.Early prognostic evaluation would aid in selecting the high-risk patients for an aggressive treatment.Awareness about risks could potentially contribute to the quality of perioperative care and optimum utilization of resources.Portsmouth modification of Physiological and operative severity for the enumeration of mortality and morbidity (P-POSSUM) and the acute physiology and chronic health evaluation Ⅱ(APACHE-Ⅱ) have been the most widely used scoring systems for emergency laparotomies.It is always better to have a single scoring system to predict outcomes and audit healthcare organizations.AIM To compare the ability of APACHE-II and P-POSSUM to predict postoperative morbidity and mortality in patients undergoing emergency laparotomy.METHODS All patients undergoing emergency laparotomy at the Tata Main Hospital,Jamshedpur between December 2013 and November 2014 were included in the study.In this observational study,P-POSSUM and APACHE-Ⅱ scoring were done,and the outcome analysis evaluated with mortality being the primary outcome.RESULTS For P-POSSUM,at a cut off value of 63 to predict mortality using receiver operating characteristics curve analysis,the area under the curve was 0.989;and for APACHE-Ⅱ,at the cut off value of 24,the area under the curve was 0.965.CONCLUSION Because the ability of APACHE-Ⅱ to predict mortality was similar to P-POSSUM and APACHE-Ⅱ does not need scoring for intra-operative findings and histopathology reports,APACHE-Ⅱ can be used pre-operatively to assess the risk in patients undergoing emergency laparotomy.However,for audit purposes,either of the two scoring systems can be used. | Deb Sanjay Nag Ankur Dembla Pratap Rudra Mahanty Shashi Kant Abhishek Chatterjee Devi Prasad Samaddar Parul Chugh | 2019 | World Journal of Clinical Cases2019，7，16： | 5 |
| 15 | Isolation and identification of bioactive antibacterial components in leaf extracts of Vangueria spinosa(Rubiaceae)显示文摘Objective:The column chromatographic fraction of ethyl acetate(EA1,EA2,EA3,EA4 and EA5) leaf extracts of Vangueria spinosa(V.spinosa) were screened for antibacterial activity and phytochemical analysis.Methods:EA3 fraction was isolated and identified by column chromatography,thin layer chromatography,spectral data analysis and phytochemical screening were used for analysis.Results:EA3 fraction was significantly active at 4 to 64 mg/L against Staphylococcus aureus,Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.1250 mg/mL.The active fraction(EA3) revealed the presence of flavonoid with retention factor value(R_f) of 0.39.The active antibacterial agent in the most potent fraction(EA3) was isolated and identified as flavonoid(-)-epicatechin-3-O-β-glucopyranoside by thin layer chromatography(TLC) and phytochemical screening.EA1 and EA2 show inhibitory activity at 4 to 64 mg/L against Staphylococcus aureus only where as fraction EA4 and EA5 do not shows any inhibitory activity within that range of concentration against any bacteria.Conclusions:The results support the ethnomedicinal use of leaf of V.spinosa for the treatment of bacterial diseases. | Soroj Kumar Chatterjee Indranil Bhattacharjee Goutam Chandra | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，1： | 5 |
| 16 | 1α,25-dihydroxyvitamin D_(3) prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital显示文摘AIM:To investigate the chemopreventive iffects of 1α,25-dihydroxyvitamin D3 in diethylnitrosamine induced,phenobarbital promoted rat hepatocarcinogenesis.METHODS:The rats were randomly divided into 6 different groups(A-F).Group A,C and E rats received a single intraperitoneal (i.p) injection of diethylnitrosamine (DEN) at a dose of 200 mg/kg body mass in 9 g/L NaCl solution at 4 wk of age ,while group B served as normal vehicle control received normal saline once.After a brief recovery of 2 WK, all the DEN treated rats were given phenobarbital (PB) at 0.5g/L daily in the basal diet till WK 20.Group C was started 4 wk prior to DEN injection and continued thereafter till wk 20 at a dose (os) twice a week.Group E received the treatment of 1α,25-(OH)2D3 at the same dose mentioned as above for 15 wk.The rats in group D and F received 1α,25-(OH)2D3 alone as in group C without DEN injection.RESULTS:The comet assay showed statistically higher mean values for length to width ratios (L:W) of DNA mass and tailed cells (p<0.01;p<0.01 respectively) in DEN treated rats as compared to their normal controls.Continuous supplementation of 1α,25-dihydroxyvitaminD3 showed a significant (p<0.01) decrease in L:W ratio of DNA mass tailed cells.Furthermore,1α,25-(OH)2D3 supplementations elevated the super oxide dismutase (SOD) activety,hepatic malondialdehyde(MDA) levle,reduced glutathione (GSH) and glutathione S-transferase (GST) activety (p<0.01,p<0.01,p<0.05 and p<0.05 respectively).As an endpoint marker histological changes were observed to establish the chemopreventive effects of 1α,25-dihydroxyvitaminD3.CONCLUSION:Supplementations of 1α,25-(OH)2D3 has a marked protection against hepatic nodelogenesis,antioxidant enzymes and DNA damages in DEN induced rat hepatocarcinogenesis promoted by phenobarbital. | Mahendrakumar Chandrasekharappa Banakar Suresh Kanna Paramasivan Mitali Basu Chattopadhyay Subrata Datta Prabir Chakraborty Malay Chatterjee Kalaiselvi Kannan Elayaraja Thygarajan | 2004 | World Journal of Gastroenterology2004，10，9： | 5 |
| 17 | Saline-Sodic Soils:Potential Sources of Nitrous Oxide and Carbon Dioxide Emissions?显示文摘Increasing salt-affected agricultural land due to low precipitation,high surface evaporation,irrigation with saline water,and poor cultural practices has triggered the interest to understand the influence of salt on nitrous oxide(N_2O) and carbon dioxide(CO_2)emissions from soil.Three soils with varying electrical conductivity of saturated paste extract(EC_e)(0.44-7.20 dS m^(-1)) and sodium adsorption ratio of saturated paste extract(SARe)(1.0-27.7),two saline-sodic soils(S2 and S3) and a non-saline,non-sodic soil(S1),were incubated at moisture levels of 40%,60%,and 80%water-filled pore space(WFPS) for 30 d,with or without nitrogen(N)fertilizer addition(urea at 525 μg g^(-1) soil).Evolving CO_2 and N2 O were estimated by analyzing the collected gas samples during the incubation period.Across all moisture and N levels,the cumulative N_2O emissions increased significantly by 39.8%and 42.4%in S2 and S3,respectively,compared to S1.The cumulative CO_2 emission from the three soils did not differ significantly as a result of the complex interactions of salinity and sodicity.Moisture had no significant effect on N_2O emissions,but cumulative CO_2 emissions increased significantly with an increase in moisture.Addition of N significantly increased cumulative N_2O and CO_2 emissions.These showed that saline-sodic soils can be a significant contributor of N_2O to the environment compared to non-saline,non-sodic soils.The application of N fertilizer,irrigation,and precipitation may potentially increase greenhouse gas(N2O and CO_2) releases from saline-sodic soils. | Upasana GHOSH Resham THAPA Thomas DESUTTER HE Yangbo Amitava CHATTERJEE | 2017 | Pedosphere2017，27，1： | 5 |
| 18 | Human immunodeficiency virus/acquired immune deficiency syndrome: Using drug from mathematical perceptive显示文摘Entry of acquired immune deficiency syndrome virus into the host immune cell involves the participation of various components of host and viral cell unit. These components may be categorized as attachment of the viral surface envelope protein subunit, gp120, to the CD4+ receptor and chemokine coreceptors, CCR5 and CXCR4, present on T cell surface. The viral fusion protein, gp41, the second cleaved subunit of Env undergoes reconfiguration and the membrane fusion reaction itself. Since the CD4+ T cell population is actively involved; the ultimate outcome of human immunodeficiency virus infection is total collapse of the host immune system. Mathematical modeling of the stages in viral membrane protein-host cell receptor-coreceptor interaction and the effect of antibody vaccine on the viral entry into the susceptible host cell has been carried out using as impulsive differential equations. We have studied the effect of antibody vaccination and determined analytically the threshold value of drug dosage and dosing interval for optimum levels of infection. We have also investigated the effect of perfect adherence of drug dose on the immune cell count in extreme cases and observed that systematic drug dosage of the immune cells leads to longer and improved lives. | Amar Nath Chatterjee Shubhankar Saha Priti Kumar Roy | 2015 | World Journal of Virology2015，4，4： | 4 |
| 19 | Leukemic stromal hematopoietic microenvironment negatively regulates the normal hematopoiesis in mouse model of leukemia显示文摘Background and Objective: Leukemic microenvironment has a major role in the progression of leukemia. Leukemic cells can induce reversible changes in microenvironmental components, especially the stromal function which results in improved growth conditions for maintaining the malignant leukemic cells. This study aimed to investigate the survival advantage of leukemic cells over normal hematopoietic cells in stromal microenvironment in long term. Methods: The mice were injected intraperitoneally with N-N’ ethylnitrosourea (ENU) to induce leukemia; the mice received injection of normal saline were used as control. At 180 days after ENU induction, the mice were killed and the bone marrows were cultured for 19 days. Colony-forming assays were used to analyze the formation of various cell colonies. The expression of Sca-1, CD146, VEGFR2, CD95, pStat3, pStat5, and Bcl-xL in marrow cells were detected by flow cytometry. Results: Long-term leukemic bone marrow culture showed abnormal elongated stromal fibroblasts with almost absence of normal hematopoietic cells. Adherent cell colonies were increased, but CFU-F and other hematopoietic cell colonies were significantly decreased in leukemia group (P< 0.001). Primitive progenitor-specific Sca-1 receptor expression was decreased with subsequent increased expression of CD146 and VEGFR-2 in leukemic bone marrow cells. Decreased Fas antigen expression with increased intracellular pStat3, pStat5 and Bcl-xL proteins were observed in leukemic bone marrow cells. Conclusions: Stromal microenvironment shows altered morphology and decreased maturation in leukemia. Effective progenitor cells are decreased in leukemia with increased leukemia-specific cell population. Leukemic microenvironment plays a role in promoting and maintaining the leukemic cell proliferation and survivability in long term. | Pratima Basak Sumanta Chatterjee Prosun Das Madhurima Das Jacintha Archana Pereira Ranjan Kumar Dutta Malay Chaklader Samaresh Chaudhuri Sujata Law | 2010 | Chinese Journal of Cancer2010，29，12： | 3 |
| 20 | ICC density predicts bacterial overgrowth in a rat model of post-infectious IBS显示文摘AIM:To investigate the interstitial cells of Cajal(ICC) number using a new rat model.METHODS:Sprague-Dawley rats were assigned to two groups.The first group received gavage with Campylobacter jejuni(C.jejuni) 81-176.The second group was gavaged with placebo.Three months after clearance of Campylobacter from the stool,precise segments of duodenum,jejunum,and ileum were ligated in self-contained loops of bowel that were preserved in anaerobic bags.Deep muscular plexus ICC(DMP-ICC) were quantified by two blinded readers assessing the tissue in a random,coded order.The number of ICC per villus was compared among controls,Campylobacter recovered rats without small intestinal bacterial overgrowth(SIBO),and Campylobacter recovered rats with SIBO.RESULTS:Three months after recovery,27% of rats gavaged with C.jejuni had SIBO.The rats with SIBO had a lower number of DMP-ICC than controls in the jejunum and ileum.Additionally there appeared to be a density threshold of 0.12 DMP-ICC/villus that was associated with SIBO.If ileal density of DMP-ICC was < 0.12 ICC/villus,54% of rats had SIBO compared to 9% among ileal sections with > 0.12(P<0.05).If the density of ICC was < 0.12 DMP-ICC/villus in more than one location of the bowel,88% of these had SIBO compared to 6% in those who did not(P<0.001).CONCLUSION:In this post-infectious rat model,the development of SIBO appears to be associated with a reduction in DMP-ICC.Further study of this rat model might help understand the pathophysiology of postinfectious irritable bowel syndrome. | Sam-Ryong Jee Walter Morales Kimberly Low Christopher Chang Amy Zhu Venkata Pokkunuri Soumya Chatterjee Edy Soffer Jeffrey L Conklin Mark Pimentel | 2010 | World Journal of Gastroenterology2010，16，29： | 3 |
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