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| 1 | Role of the advanced glycation end products receptor in Crohn's disease inflammation显示文摘AIM:To investigate the level of mucosal expression and the involvement of the receptor for the advanced glycation end products(RAGE)in delayed apoptosis and tumor necrosis factor(TNF)-αproduction in Crohn’s disease(CD).METHODS:Surgical and endoscopic specimens from both inflamed and non-inflamed areas of the ileum and/or colon were collected from 20 and 14 adult CD patients,respectively,and used for the assessment of RAGE expression by means of immunohistochemistry and western blotting analysis.Normal tissues from 21 control subjects were used for comparison.The same polyclonal anti-human RAGE antibody(R and D System)was used in all experimental conditions.RAGE staining was quantized by a score including both the amount of positive cells and intensity of immunoreactivity;cellular pattern was also described.The effects of RAGE blocking on apoptotic rate and TNF-αproduction were investigated on immune cells freshly isolated from CD mucosa and incubated both with and without the muramyl dipeptide used as antigenic stimulus.Statistical analysis was performed via the test for trend,with regression models to account for intra-patient correlations.A 2-sided P<0.05 was considered significant.RESULTS:In inflamed areas,RAGE expression in both the epithelial and lamina propria compartments was higher than control tissues(P=0.001 and 0.021,respectively),and a cluster of positive cells were usually found in proximity of ulcerative lesions.Similar results were obtained in the lamina propria compartment of non-inflamed areas(P=0.025).The pattern of staining was membranous and granular cytosolic at the epithelial level,while in the lamina propria it was diffuse cytosolic.When evaluating the amount of protein expression by immunoblotting,a significant increase of both surface area and band intensity(P<0.0001 for both)was observed in CD inflamed areas compared to control tissue,while in non-inflamed areas a significant increase was found only for band intensity(P<0.005).Moreover,a significantly lower expression in noninflamed areas in comparison with inflamed areas was found for both surface area and band intensity(P<0.0006 for both).Finally,RAGE blocking largely affects both the apoptotic rate of mucosal cells(towards an increase in both non-inflamed and inflamed areas of P<0.001 and<0.0001,respectively)and TNF-αsecretion(towards a decrease in both non-inflamed and inflamed areas of P<0.05 and<0.01,respectively),mainly in the presence of antigenic stimulation.CONCLUSION:RAGE is up-regulated in CD,especially in inflamed areas,and it appears to play a role in the mechanisms involved in chronic inflammation. | Rachele Ciccocioppo Alessandro Vanoli Catherine Klersy Venerina Imbesi Vincenzo Boccaccio Rachele Manca Elena Betti Giuseppina Cristina Cangemi Elena Strada Roberta Besio Antonio Rossi Colomba Falcone Sandro Ardizzone Paolo Fociani Piergiorgio Danelli Gino Roberto Corazza | 2013 | World Journal of Gastroenterology2013，19，45： | 2 |
| 2 | Changes in efficiency and resource utilization after increasing experience with double balloon enteroscopy显示文摘AIM:To investigate changes in efficiency and resource utilization as a single endoscopist's experience increased with each subsequent 100 double balloon enteroscopy(DBE) procedures.METHODS:We reviewed consecutive DBE procedures performed by a single endoscopist at our center over 4 years.DBE was employed when the clinician deemed the procedure was needed for disease management.The approach(oral,anal or both) was chosen based on suspected location of the target lesion.All DBE was performed in a standard endoscopy room with a portable fluoroscopy unit.Fluoroscopy was used to aid in shortening the small intestine and reducing bowel loops.For oral DBE,measurements were taken from the incisors.For anal DBE,measurements were taken from the anal verge.Enteroscopy continued until the target lesion was reached,until the entire small intestine was examined,or until no further progress was deemed possible.The length of small intestine examined(cm),procedure duration(min),and fluoroscopy time(s) were analyzed for sequential groups of 100 DBE.Sub-groups of diagnostic and therapeutic procedures were analyzed using multivariable linear regression.RESULTS:802 consecutive DBE procedures were analyzed.For oral DBE,median [interquartile range(IQR)] length of small bowel examined was 230.8 cm(range:210-248 cm) and for anal DBE was 143.5 cm(range:100-180 cm).No significant increase in length examined was noted for either the oral or anal approach with advancing position in series.In terms of duration of procedure,the median(IQR) for oral DBE was 86 min(range:71-105 min) and for anal DBE was 81.3 min(range:67-105 min).When comparing by the position in series,there was a significant(P value < 0.001) decrease in procedure duration for both upper and lower procedures with increasing experience.Median(IQR) time of exposure to fluoroscopy for oral DBE was 190 s(114-275) compared to anal DBE which was 196.4 s(312-128).This represented a significant(P value < 0.001) decrease in the amount of fluoroscopy used with increasing position in series.For both oral and anal DBE,fluoroscopy time was reduced by greater than 50% over the course of 802 total procedures performed.Sub-group analysis was conducted on therapeutic and diagnostic groups.Out of 802 procedures,a total of 434 were considered therapeutic.Argon plasma coagulation was by far the most common therapeutic intervention performed.There was no evidence of a difference in length examined or fluoroscopy exposure among oral DBE for diagnostic and therapeutic procedures,P = 0.91 and P = 0.32 respectively.The median(IQR) for length was 235 cm(range:178-280 cm) for diagnostic vs 230 cm(range:180-275 cm) for therapeutic procedures;additionally,fluoroscopy time median(IQR) was 180 s(range:110-295 s) and 162 s(range:102-263 s) for no intervention and intervention.However,there was a significant difference in procedure duration among oral DBE(P < 0.001).The median(IQR) was 80 min(range:60-97 min) and 94 min(range:77-110 min) for diagnostic and therapeutic interventions respectively.CONCLUSION:For a single endoscopist,increased DBE experience with number of performed procedures is associated with increased efficiency and decreased resource utilization. | Neal C Patel William C Palmer Kanwar R Gill David Cangemi Nancy Diehl Mark E Stark | 2013 | World Journal of Gastrointestinal Endoscopy2013，5，3： | 2 |
| 3 | Fibulin-1 is a marker for arterial extracellular matrix alterations in type 2 diabetes显示文摘 | Cangemi C Skov V Poulsen Mk | 2011 | Clin Chem2011，57，11： | 1 |
| 4 | Is NOX2 upregulation implicated in myocardial injury in patients with pneumonia显示文摘 | Cangemi R Calvieri C Bucci T | 2014 | Antioxid Redox Signal2014，20，18： | 1 |
| 5 | Prognositic value of ferritin,neuron-specific enolase,lactate dehydrogenase,and urinary and plasmatic catecholamine metabolites in children with neuroblastoma显示文摘 | Cangemi G Reggiardo G Barco G | 2012 | Onco Targets Ther2012，5，： | 1 |
| 6 | In vitro antibacterial effect of different irrigating solutions on Enterococcus faecalis显示文摘 | Bulacio Mde L Cangemi R Cecilia M | 2006 | Acta Odontol Latinoam2006，19，2： | 1 |
| 7 | Intestinal lschemia in the Elderly显示文摘 | Cangemi JR Picco MF | 2009 | Gastroen- terol Clin North Am2009，38，7： | 1 |
| 8 | A review on cancer psychospirtual status interactions显示文摘 | Lissoni P Cangemi P Pirato D | 2001 | Neuroendocrinol Lett2001，22，3： | 1 |
| 9 | Pioglitazone,vitamin E ,or placebo for nonal- coholic steatohepatitis显示文摘 | Violi F Cangemi R | 2010 | N Engl J Med2010，363，12： | 1 |
| 10 | Pioglitazone, Pioglitazone, vitamin E, or placebo for nonalcoholic steatohepatitis显示文摘 | Violi F Cangemi R | 2010 | N Engl J Med2010，363，12： | 1 |
| 11 | TOZAL Study: an open ease control study of an oral antioxidant and omega-3 supplement for dry AMD 显示文摘 | Cangemi FE | 2007 | BMC Ophthafmo]2007，7，： | 1 |
| 12 | Lower mortality rate in elderly patients with community-onset pneumonia on treatment with aspirin显示文摘 | Falcone M Russo A Cangemi R | 2015 | J Am Heart Assoc2015，4，00： | 1 |
| 13 | Cholesterol-adjusted vitamin E serum levels are associated with cardiovascular events in patients with non-valvular atrial fibrillation 显示文摘 | Cangemi R Pignatelli P Camevale R | 2013 | Int J Cardiol2013，168，4： | 1 |
| 14 | Lower mortality rate in elderly patients with community-onset pneumonia on treatment with aspirin显示文摘 | Falcone M Russo A Cangemi R | 2015 | J Am Heart Assoc2015，4，00： | 1 |
| 15 | Protective effect of intranasally inoculated LactobaciUus fermentum against Streptococcus pneumoniae challenge on the mouse respiratory tract显示文摘 | Cangemi de Gutierrez R Santos V Nader-Maclas ME | 2001 | FEMS Immunol Med Microbiol2001，31，3： | 1 |
| 16 | Randomized controlled trial of recombinant alpha-2a-interferon for chronic hepatitis C显示文摘 | David D. Douglas MD Dr. Jorge Rakela MD Hsiang Ju Lin DSc F. Blaine Hollinger MD Howard F. Taswell MD Albert J. Czaja MD John B. Gross MD Monte L. Anderson MD Kevin Parent MD C. Richard Fleming MD John R. Cangemi MD Peter C. O’Brien PhD Pauline E. Powis R | 1993 | Digestive Diseases and Sciences1993，，4： | 1 |
| 17 | Small bowel tumors discovered during double-balloon enteroscopy: analysis of a large prospectively collected single-center database显示文摘 | Cangemi DJ Patel MK Gomez V | 2013 | J Clin Gastroenterol2013，47，： | 1 |
| 18 | Effect of intranasal administration of Lactobacillus fermentum on the respiratory tract of mice显示文摘 | CANGEMI de Gutierrez RV SANTOS de Araoz ME NADERMACIAS | 2000 | Biol Pharm Bull2000，23，： | 1 |
| 19 | Protective effect of intranasally inoculated Lactobacillus fermentum against Streptococcus pneumoniae challenge on the mouse respiratory tract显示文摘 | CANGEMI de Gutierrez R SANTOS V NADER-MACIAS ME | 2001 | FEMS Immunol Med Microbiol2001，31，3： | 1 |
| 20 | A review on cancer-psychospiritual status interactions显示文摘 | LISSONI P CANGEMI P PIRATO D | 2001 | Neuro Endocrinol Lett2001，22，3： | 1 |
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