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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Scientific and clinical challenges in sepsis 显示文摘 | Ulloa L Brunner M Ramos L | 2009 | Curr Pharm Des2009，15，16： | 1 |
| 3 | On the Relationship Between Double Bounce and the Ori- entation of Buildings in VHR SAR Images显示文摘 | FERRO A BRUNNER D BRUZZONE L | 2011 | IEEE Geoscienee and Remote Sensing Letters2011，8，4： | 1 |
| 4 | Ftuorescence in situ hybridiz- ation for detecting upper urinary tract tumorse a preliminary report显示文摘 | Akkad T Brunner A Pallwein L et aI | 2007 | Urology2007，70，： | 1 |
| 5 | Small animal model of weightlessness for pharmacokinetic evaluation 显示文摘 | FELDMAN S BRUNNER L J | 1994 | Clin Pharmacol1994，34，： | 1 |
| 6 | In vitro study of ingested coins: leave them or retrieve them 显示文摘 | Rebhandl W Milassin A Brunner L Steffan I Benk T H? rmann M Burtscher J | 2007 | Pediatr Surg2007，42，10： | 1 |
| 7 | Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver显示文摘 | BRUNNER A L JOHNSON D S KIM S W | 2009 | Genome Res2009，19，6： | 1 |
| 8 | Deposition of sol-gel derived membranes on α-Al2O3 hollow fibers by a vacuum-assisted dip-coating process显示文摘 | Pan X L Stroh N Brunner H | 2003 | Journal of Membrane Science2003，226，12： | 1 |
| 9 | Flu- orescence in situ hybridization for detecting upper uri- nary tract tumors--a preliminary report显示文摘 | AKKAD T BRUNNER A PALLWEIN L | 2007 | Urology2007，70，： | 1 |
| 10 | A comparison of FE-BE coupling schemes for large-scale problems with fluid-structure interaction显示文摘 | BRUNNER D JUNGE M GAUL L | | 0，，： | 1 |
| 11 | Photonic information processing beyond Turing: An optoelectronic implementation of reservoir computing显示文摘 | L Larger M C Soriano D Brunner | 2012 | Opt Express2012，20，3： | 1 |
| 12 | Nutrient balances for Danube counties:a strategic analysis显示文摘 | SOMLYODY L BRUNNER P H KROIBH | 1999 | Wat Sci Tech1999，40，10： | 1 |
| 13 | Pd / ceramic hollow fibers for H2 separation 显示文摘 | Pan X L Stroh N Brunner H | 2003 | Separation and Purification Technology2003，32，13： | 1 |
| 14 | Transgene x environmentinteractions in genetically modified wheat 显示文摘 | ZellerS L Kalinina 0 Brunner S | 2010 | Plos One2010，5，7： | 1 |
| 15 | Unequal death in T helper cell (Th)1 and Th2 effectors:Th1, hut not Th2, effectors undergo rapid Fas-FasL-mediated apoptosis显示文摘 | ZHANG X BRUNNER T CARTER L | 1997 | J Exp Med1997，185，10： | 1 |
| 16 | Scientific and clinical challenges in sepsis显示文摘 | ULLOA L BRUNNER M RAMOS L | 2009 | Curr Pharm Des2009，15，16： | 1 |
| 17 | Functional analysis and cell-specific expression of a phosphate transporter from tomato 显示文摘 | Daram P Brunner S Persson B L | 1998 | Planta1998，206，2： | 1 |
| 18 | Comparative evaluation of urokinase-type plasminogen activator receptor expression in primary breast carcinomas and on metastatic tumor cells显示文摘 | HEMSEN A RIETHDORF L BRUNNER N | 2003 | Int J Cancer2003，107，6： | 1 |
| 19 | Building Height Retrieval From VHR SAR Imagery Based on an Iterative Simulation and Matching Technique显示文摘 | Brunner D Lemoine G Bruzzone L | 2010 | Geoscience and Remote Sensing IEEE Transactions on2010，48，3： | 1 |
| 20 | Renin, angiotensin and aldosterone system in pathogenesis and management of hypertensive vascular disease显示文摘 | Laragh J H L Bear H R Brunner | 1972 | Am J Med1972，52，： | 1 |
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