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295篇 您的检索式：作者名="Brunner D"

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	题名	作者	年代	出处	被引量
1	Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype.	C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf	2014	World Journal of Cardiology2014，6，4：	3
2	Comparison of nanoscale and microscale bioactive glass on the properties of P(3HB)/Bioglass(R) composites显示文摘	MISRA S K MOHN D BRUNNER T J	2008	Biomaterials2008，29，12：	1
3	On the Relationship Between Double Bounce and the Ori- entation of Buildings in VHR SAR Images显示文摘	FERRO A BRUNNER D BRUZZONE L	2011	IEEE Geoscienee and Remote Sensing Letters2011，8，4：	1
4	Associations of C- reactive protein and interleukin-6 with cognitive symptoms of depression: 12-year follow-up of the Whitehall II study 显示文摘	Gimeno D Kivimaki M Brunner EJ	2009	Psychol Med2009，39，3：	1
5	Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes 显示文摘	Methe H Brunner S Wiegand D	2005	J Am Coil Cardiol2005，45，12：	1
6	Bacterial degradation of dichloromethane显示文摘	Brunner W Staub D Leisinger T	1980	Applied and Environmental Microbiology1980，40，5：	1
7	Toward an integrated assessment of the impacts of extreme wind events on Barrow,Alaska显示文摘	Lynch A H Curry J A Brunner R D	2004	Bulletin of American Meteorology Society2004，85，：	1
8	Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver显示文摘	BRUNNER A L JOHNSON D S KIM S W	2009	Genome Res2009，19，6：	1
9	Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes显示文摘	Brunner S Wiegand D	2005	J Am Coll Cardiol2005，45，：	1
10	Sodium balance-neutral sodium profiling does not improve dialysis tolerance 显示文摘	Iselin H Tsinalis D Brunner FP	2001	Swiss Med Wkly2001，131，4344：	1
11	A comparison of FE-BE coupling schemes for large-scale problems with fluid-structure interaction显示文摘	BRUNNER D JUNGE M GAUL L		0，，：	1
12	Photonic information processing beyond Turing: An optoelectronic implementation of reservoir computing显示文摘	L Larger M C Soriano D Brunner	2012	Opt Express2012，20，3：	1
13	Lifetime course ofmyasthenia gravis显示文摘	Grob D Brunner N Namba T	2008	Muscle Nerve2008，37，2：	1
14	Enhanced T-help- er-1 lymphocyte activation patterns in acute coronary syn- dromes显示文摘	Methe H Brunner S Wiegand D	2005	J Am Colt Cardiol2005，45，12：	1
15	Facial Sweet syndrome mimicking photoallergic contact dermatitis 显示文摘	Johannemann D Brunner M Zouboulis CC	2009	J Dtsch Dermatol Ges2009，7，8：	1
16	Arterial stiffness, physi- cal function,and functional limitation: the Whitehall Ⅱ Study显示文摘	Brunner E J Shipley M J Witte D R	2011	Hypertension2011，57，5：	1
17	Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods显示文摘	van der Valk J Brunner D De Smet K		0，，04：	1
18	Prediction-based optimal power management in a fuel cell/battery plug-in hybrid vehicle显示文摘	P Bubna D Brunner S G Advani		0，，19：	1
19	Using color in machine vision systems for wood processing 显示文摘	Brunner C C Shaw G B Butler D A	1990	Wood Fiber Sci1990，22，5：	1
20	Breast milk leptin andadiponectin in relation to infant body composition up to 2 years显示文摘	Brunner S Schmid D Zang K	2015	Pediatric Obesity2015，10，1：	1

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