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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004,30,4: | 2 |
| 2 | Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis显示文摘 | Wies I Brunner S Henninger J | 2000 | Lancet2000,355,2534: | 1 |
| 3 | Solublevascular endothelial growth factor levels in patients with primarycolorectal carcinoma : the Danish RANX05 colorectal Cancer StudyGroup显示文摘 | WERTHER K CHRISTENSEN I J BRUNNER N | 2000 | Eur J Surg Oncol2000,26,7: | 1 |
| 4 | In vitro study of ingested coins: leave them or retrieve them 显示文摘 | Rebhandl W Milassin A Brunner L Steffan I Benk T H? rmann M Burtscher J | 2007 | Pediatr Surg2007,42,10: | 1 |
| 5 | Heavy metalaccumulation and phytostabilisafion potential of tree fine roots in a contaminated soil显示文摘 | Brunner I Luster J Giienthardt-Goerg M S | 2008 | Environmental Pollution2008,152,3: | 1 |
| 6 | Chemical properties of solid biofuels - significance and impact显示文摘 | Obernberger I Brunner T Bairnthaler G | 2006 | Biomass and Bioenergy2006,30,11: | 1 |
| 7 | Soluble vascular endothelial growth factor level in patients with primary colorectal carcinoma显示文摘 | Werther K Christensen I J Brunner N | 2000 | Eur J Surg Oncol2000,26,7: | 1 |
| 8 | Reduction of biomass decay rate under anoxic and anaerobic conditions显示文摘 | Siegrist I Brunner G Koch G | 1999 | Wat Sci Tech1999,39,1: | 1 |
| 9 | A case of nasal leiomyosarcoma: the first comparative genomic hybridization analysis显示文摘 | Peters I Brunner C Urbschat S | 2003 | Kulak Burun Bogaz Ihtis Derg2003,11,4: | 1 |
| 10 | A case of nasal leiomyosarco- ma:the first comparative genomic hybridization analysis 显示文摘 | Peters I Brunner G Urbschat S | 2003 | Kulak Burun Bogaz Ihtis Derg2003,11,4: | 1 |
| 11 | Validating in- ternal controls for quantitative plant gene expression studies显示文摘 | Brunner A M Yakovlev I A Strauss S H | 2004 | BMC Plant Biology2004,4,1: | 1 |
| 12 | Aluminium resistance of Norway spruce: Root exudation versus immobilization in roots显示文摘 | Heim A Brunner I Frey B | 2001 | Plant Nutrition2001,9,: | 1 |
| 13 | Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis 显示文摘 | Wies I Brunner S Henninger J | 2000 | Lancet2000,355,9214: | 1 |
| 14 | Urinary neutrophil gelatinase -associated lipocalin as a biomarker of nephritis in childhood-onset systemic lupus erythematosus 显示文摘 | BRUNNER H I MUELLER M RUTHERFORD C | 2006 | Arthritis Rheum2006,54,8: | 1 |
| 15 | Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis显示文摘 | Wies I Brunner S Henninger J | 2000 | Lancet2000,355,: | 1 |
| 16 | Wood-ash recycling affects forest soil and tree fine-root chemistry and reverses soil acidification显示文摘 | Brunner I Zimmermann S Zingg A Blaser P | 2004 | Plant & Soil2004,267,: | 1 |
| 17 | Soluble vascular endothelial growth factor levels in patients with primary colorectal carcinoma显示文摘 | Werther K Christensen I J Brunner N | 2000 | Eur J Surg Oncot2000,26,: | 1 |
| 18 | Thermodynamic equilibrium calculations concerning the removal of heavy metals from sewage sludge ash by chlorination显示文摘 | FRAISSLER G JOLLER M MATTENBERGER H BRUNNER T OBERNBERGER I | 2009 | Chemical Engineering and Processing:Process Intensification2009,48,1: | 1 |
| 19 | Altered expression of beta-catenin/E-cadherin in meningiomas显示文摘 | Romeike BF Jung I | 2006 | Histopathology2006,49,2: | 1 |
| 20 | Difference in disease features between child- hood-onset and adult-onset systemic lupus erythematosus 显示文摘 | Brunner H I | 2008 | Arthritis Rheum2008,58,2: | 1 |