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| 1 | Distribution and relative activity of matrix metalloproteinase-2 in human coronal dentin显示文摘The presence of matrix metalloproteinase-2 (MMP-2) in dentin has been reported, but its distribution and activity level in mature human coronal dentin are not well understood. The purpose of this study was to determine the MMP-2 distribution and relative activity in demineralized dentin. Crowns of twenty eight human molars were sectioned into inner (ID), middle (MD), and outer dentin (OD) regions and demineralized. MMP-2 was extracted with 0.33 mol·L -1 EDTA/2 mol·L -1 guanidine-HCl, pH 7.4, and MMP-2 concentration was estimated with enzyme-linked immunoabsorbant assay (ELISA). Further characterization was accomplished by Western blotting analysis and gelatin zymography. The mean concentrations of MMP-2 per mg dentin protein in the dentin regions were significantly different (P=0.043): 0.9 ng (ID), 0.4 ng (MD), and 2.2 ng (OD), respectively. The pattern of MMP-2 concentration was OD>ID>MD. Western blotting analysis detected ~66 and ~72 kDa immunopositive proteins corresponding to pro-and mature MMP-2, respectively, in the ID and MD, and a ~66 kDa protein in the OD. Gelatinolytic activity consistent with MMP-2 was detected in all regions. Interestingly, the pattern of levels of Western blot immunodetection and gelatinolytic activity was MD>ID>OD. The concentration of MMP-2 in human coronal dentin was highest in the region of dentin that contains the dentinoenamel junction and least in the middle region of dentin. However, levels of Western blot immunodetection and gelatinolytic activity did not correlate with the estimated regional concentrations of MMP-2, potentially indicating region specific protein interactions. | Lee W. Boushell Masaru Kaku Yoshiyuki Mochida Mitsuo Yamauchi | 2011 | International Journal of Oral Science2011，3，4： | 2 |
| 2 | Near-infrared spectroscopy for monitoring muscle oxygenation显示文摘 | Boushel R Piantadosi CA | 2000 | Acta Physiol Scand2000，168，： | 1 |
| 3 | Tissue engineering strategies for the regeneration of orthopedic interfaces显示文摘 | Lu HH Subramony SD Boushell MK | | 0，，06： | 1 |
| 4 | Cerebral desaturation during exercise reversed by 02 supplementation 显示文摘 | NIELSEN H B BOUSHEL R MADSEN P | 1999 | Am J Physiol1999，277，32： | 1 |
| 5 | On the mechanisms that limit oxygen uptake during exercise in acute and chronic hypoxia: role of muscle mass显示文摘 | Calbet J A Radegran G Boushel R | 2009 | J Physiol2009，587，2： | 1 |
| 6 | Near-infrared spectroscopy for monitoring muscle oxygenation 显示文摘 | Boushel R Piantadosi C A | 2000 | Acta Physiol Scand2000，168，4： | 1 |
| 7 | Increased matrixmetallo -proteinase -2 and bone sialoprotein response to humancoronal caries显示文摘 | Boushell LW Nagaoka H Nagaoka H | 2011 | Caries Res2011，45，5： | 1 |
| 8 | Near-infrared spectroscopy determined brain and muscle oxygenation during exercise with normal and resistive breathing 显示文摘 | Nielsen HB Boushel R Secher NH | 2009 | Acta Physiological Scandinavia2009，171，8： | 1 |
| 9 | Immunohistochemical localization of ma-trixmetalloproteinase-2 in human coronal dentin显示文摘 | Boushell LW Kaku M Mochida Y | | 0，，： | 1 |
| 10 | Ex vivo measures of muscle mitochondrial capacity reveal quantitative limits of oxygen delivery by the cir?culation during exercise显示文摘 | Boushel R Saltin B | 2013 | Int J Biochem Cell Bioi2013，45，1： | 1 |
| 11 | Monitoring tissue oxygen availability with near infrared spectroscopy(NIBS)in health and disease显示文摘 | Boushel R Langberg H Olesen J | 2001 | Scand J Med Sci Sports2001，11，4： | 1 |
| 12 | Near-infrared spectroscop for monitoring muscule ox-ygenation显示文摘 | Boushel R Piantadosi CA | 2000 | Acta Physiol Scand2000，168，： | 1 |
| 13 | Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects显示文摘 | Steen Larsen Joachim Nielsen Christina Neigaard Hansen Lars Bo Nielsen Flemming Wibrand Nis Stride Henrik Daa Schroder Robert Boushel J?rn Wulff Helge Flemming Dela Martin Hey‐Mogensen | 2012 | The Journal of Physiology2012，，14： | 1 |
| 14 | Determinants of maximal oxygen uptake in severe acute hypoxia显示文摘 | CALBET J A BOUSHEL R RADEGRAN G | 2003 | Am J Physiol Regul Integr Comp Physiol2003，284，2： | 1 |
| 15 | Increased matrix metallo-proteinase-2 and bone sialoprotein response to human coro- nal caries显示文摘 | Boushell LW Nagaoka H Nagaoka H | 2011 | Caries Res2011，45，5： | 1 |
| 16 | Metabolic control of muscle blood flow during exercise in humans显示文摘 | Boushel R | 2003 | J Appl Physiol2003，28，5： | 1 |
| 17 | Combined inhibition of nitric oxide and prostaglandins reduces human skeletal muscle blood flow during exercise显示文摘 | Boushel R Langberg H Gemmer C | 2002 | J Physiol2002，543，2： | 1 |
| 18 | Monitoring tissue oxygen availability with near infrared spectroscopy(NIRS)in health and disease显示文摘 | Boushel R Langberg H Olesen J | 2001 | Scand J Med Sci Sports2001，11，4： | 1 |
| 19 | Regional blood flow during exercise in humans measured by near - infrared spectroscopy and indocyanine green 显示文摘 | Boushel R Langberg H Olesen J | 2000 | J Appl Physiol2000，89，： | 1 |
| 20 | On the Mechanisms That Limit Oxygen Uptake During Exercise in Acute and Chronic Hy- poxia Role of Muscle Mass显示文摘 | CalbetJ A Radegran G Boushel R etal | 2010 | High Ah Med Biol2010，11，2： | 1 |
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