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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Restoration Prospects for Heitutan Degraded Grassland in the Sanjiangyuan显示文摘In many ecosystems ungulates have coexisted with grasslands over long periods of time. However, high densities of grazing animals may change the floristic and structural characteristics of vegetation, reduce biodiversity, and increase soil erosion, potentially triggering abrupt and rapid changes in ecosystem condition. Alternate stable state theory provides a framework for understanding this type of dynamic. In the Sanjiangyuan atop the Qinghai-Tibetan plateau (QTP), grassland degradation has been accompanied by irruptions of native burrowing animals, which has accentuated the loss of ground cover. Severely degraded areas of alpine meadows are referred to as 'Heitutan'. Here, using the framework of alternate stable state theory, we describe the proximate and ultimate drivers of the formation of Heitutan on the QTP, and we assess prospects for recovery, in relation to the degree of biophysical alteration, of these alpine meadows. Effective rehabilitation measures must address the underlying causes of degradation rather than their symptoms. Heitutan degradation is not uni-causal. Rather it reflects different mechanisms operating at different spatio-temporal scales across this vast region. Underlying causes include overly aggressive exploitation of the grasslands (e.g. overgrazing), amplification of grazing and erosion damage by small mammals when outbreaks occur, and/or climate change. Given marked variability in environmental conditions and stressors, restorative efforts must vary across the region. Restoration efforts are likely toyield greatest success if moderately and severely degraded areas are targeted as the first priority in management programmes, before these areas are transformed into extreme Heitutan. | LI Xi-lai PERRY LW George BRIERLEY Gary GAO Jay ZHANG Jing YANG Yuan-wu | 2013 | Journal of Mountain Science2013，10，4： | 23 |
| 3 | Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Thl cells within immune organ of mouse IgA nephropathy显示文摘 | Jia, LW Wang, C Kong, HW Yang, J Li, FL Lv, S Xu, GW | 2007 | 中国生物学文摘2007，21，11： | 19 |
| 4 | Chemical composition of gas from mantle xenoliths in alkali- basalt from Damaping, Hebei显示文摘WE have measured the chemical composition of the gas extracted from ortho- and clino-pyroxenes in spinel lherzolite xenolith, and from black pyroxenolite xenolith in Cenozoic alkah-basalt from Damáping, Wanquan County, Hebei Province, by pyrolystic method. | Liu, G Wang, XB Li, LW | 1997 | Chinese Science Bulletin1997，42，6： | 11 |
| 5 | Effects of HBV gene variations on disease development and antiviral therapy for patients with chronic hepatitis B显示文摘Viral variation may change pathogenicity, escape immunity, lead to persistence infection, and cause drug resistance against antiviral therapy. This study was undertaken to investigate the effects of HBV gene variation on the progression of disease and on the efficacy of antiviral therapy for patients with chronic hepatitis B(CHB). METHODS:Hepatitis B virus (HBV) gene mutational sites were detected using gene chip in selected hepatitis B patients. RESULTS:In the patients HBeAg did not show serologic conversion or HBeAg(-)/anti-HBe(+), but their HBV DNA remained positive 24 weeks after α-interferon therapy, which was associated with mutations of nt1896, nt1814, nt1762 and nt1764. In the patients, that HBV DNA levels decreased or were undetectable, but rebounded later after antiviral therapy by lamivudine was associated with mutations of aa528 and(or) aa552(i.e.YMDD mutation), which resulted in lamivudine-resistance. YMDD mutation was prone to occur 52 weeks after lamivudine therapy in some chronic hepatitis B patients (26.4%). Nt1896 mutation was common in most chronic hepatitis B patients (68.5%). Chronic severe hepatitis, cirrhosis, and primary liver carcinoma were related to the mutations of nt1896, nt1762 and nt1764. CONCLUSIONS:HBV gene mutations could aggravate patient’s condition and affect the efficacy of antiviral therapy. The regular detection of HBV gene mutation is helpful for identification of disease prognosis and adjustment of therapeutic strategy. | Shenzhen Eastlake Hospital, Shenzhen 518020, China (Chen JY) and National Key Laboratory of Virology (Wuhan University) Department of Infectious Diseases, Renmin Hospital, Wuhan University, Wuhan 430060, China(Wang LW, Sun XM and Gong ZJ) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 8 |
| 6 | Role of sonic hedgehog in maintaining a pool of proliferating stem cells in the human fetal epidermis显示文摘 | Zhou JX Jia LW Liu WM Miao CL Liu S Cao YJ Duan EK | 2006 | 中国生物学文摘2006，20，5： | 8 |
| 7 | 血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性显示文摘【据Joural of Viral Hepatitis 2020年8月报道】题:血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性(作者Chen YP等)研究表明,FIB-4及APRI等常规指数诊断慢性乙型肝炎肝硬化、显著肝纤维化在不同研究中存在差异。近期,研究者们也先后提出了多项新的常规指数,包括红细胞体积分布宽度-血小板比值(RPR)、谷氨酰转肽酶-血小板比值(GPR)及简易肝纤维化指数(eLIFT)。为评估ALT水平对这些常规指数诊断表现的影响,1005例慢性乙型肝炎患者被纳入回顾性分析,这些患者均已接受肝活检及常规血液检查。在该研究中,似然比10、0.1的对应值分别被确定为确认诊断、排除诊断界值。 | 林小钰 陈永鹏 CHEN YP HUANG LW LIN XY | 2020 | 临床肝胆病杂志2020，36，9： | 7 |
| 8 | A chemical index of weather-ing without effect of wind sorting: Fe/Mg ratios in the acid-insoluble phases of loess deposits显示文摘Chemical analysis of acid-insoluble fractions in loess and paleosols shows that concentrations of Fe and Mg were under control of wind sorting and post-depositional weathering-pedogenesis. The former caused Fe and Mg concentrated in the finer grain-size fractions, displaying synchronous variations, while the latter made Fe and Mg separated, leading to Fe retained in the weathered section and Mg leached out. Therefore, Fe/Mg ratios in the acid insoluble fraction of loess and paleosols can eliminate the effect of wind sorting and serve as an excellent proxy record on intensity of weathering-pedogenesis. Based on calculation, leaching percentage of Mg in the paleosol S1 from the Luochuan, Xifeng and Huanxian sections is 15%, 11% and 2%, respectively, and on average 9% for the paleosols S2—S14 from the Luochuan section, with the highest value amounting to 22% in S5-1, suggesting the strongest weathering-pedogenesis. | Liu, LW Chen, J Wang, HT Chen, Y | 2001 | Chinese Science Bulletin2001，46，16： | 5 |
| 9 | 高危心血管病老年人血压变异性的相关因素显示文摘诊问血压变异性是心血管病的独立预测因素。该研究探讨老年人血压变异的相关因素。方法:在普伐他汀干预高危老年人的前瞻性研究( pravastatin inelderly individuals at risk of vascular disease, PROS-PER)中,分析70~82岁有心血管病史或危险因素的男性和女性者3794人。 | Poortvliet RK Lloyd SM Ford I Sattar N de Craen AJ Wijsman LW Mooijaart SP Westendorp G Jukema JW de Ruijter W Gussekloo J Stott DJ | 2015 | 中华高血压杂志2015，23，10： | 3 |
| 10 | A randomized double-blinded sham-controlled trial of electroencephalogram-guided transcranial magnetic stimulation for obsessive-compulsive disorder显示文摘 | Ma xY HuangYQ Liao LW Jin .Y | 2014 | Chinese Medical Journal2014，，4： | 3 |
| 11 | 国际口腔颌面创伤外科发展史显示文摘自古以来创伤便一直伴随人类左右。作为口腔颌面外科的常见病和多发病,口腔颌面创伤是口腔颌面外科的重要内容之一。从最早的文字记载开始,口腔颌面创伤外科经历了漫长的发展历程。如今,口腔颌面创伤外科取得的长足进步离不开无数前人的尝试和努力。本文总结梳理了国际口腔颌面创伤外科的发展历程,追溯其中具有里程碑意义的事件,为广大读者提供参考。 | 黄秀玲(编译) 张益(审校) stoelinga pjw john lw | 2017 | 中国口腔颌面外科杂志2017，15，2： | 3 |
| 12 | Joint Semantic and Feature Based Image Retrieval Using Relevance Feedback显示文摘 | LU Y ZHANG HJ YIN LW | 2003 | IEEE Transaction on Multimedia2003，5，3： | 2 |
| 13 | Outcomes and perioperative hyperglycemia in patients with or without diabetes mellitus undergoing coronary artery bypass grafting 显示文摘 | Estrada CA Young JA Nifong LW | 2003 | Ann Thorac Surg2003，75，5： | 2 |
| 14 | Longitudinal stability of visual evoked potentials in children and adolescents with hydrocephalus 显示文摘 | Desch LW | 2001 | Dev Med Child2001，43，： | 2 |
| 15 | MR image artifacts from periodic motion显示文摘 | Michael LW Henkelman RM | 1985 | Med Phys1985，12，2： | 2 |
| 16 | Paraventricular nucleus:a site for integration of neuroendocrine and autonomic mechanisms显示文摘 | Swanson LW Sawchenko PE | 1980 | Neuroendocrinology1980，31，： | 2 |
| 17 | Intra-articular hyaluronan (hyaluronic acid) and hylans for the treatment of osteoarthritis:mechanisms of action显示文摘 | Moreland LW | | Arthritis Research & Therapy0，，： | 2 |
| 18 | Induction of alpha-fetoprotein- specific CD4- and CD8-mediated T-cell response using RNA-trans-fected dendritic cells显示文摘 | Zhang HM Zhang LW Ren J | 2006 | Cell Immunol2006，239，2： | 1 |
| 19 | Costimulatory blockade in patients with rheumatoid arthritis: a pilot, dose-finding, double-blind, placebo-controlled clinical trial evaluating CTLA-4Ig and LEA29Y eighty-five days after the first infusion显示文摘 | MORELAND LW ALTEN R van den BOSCH F l | 2002 | Arthritis Rheum2002，46，6： | 1 |
| 20 | Penetrating injuries of the pleural cavity显示文摘 | Luvuma FM and Baker LW | 1994 | Thorax1994，39，： | 1 |
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