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| 1 | Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells显示文摘Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1 ) 的主要 brassinosteroid (BR ) 受体在 BR 发信号,而是在 BRI1 成为主观和集会状态遗体上位于 BR 的效果下面的分子的机制起基本作用不清楚。这里,我们使用了可变角度总数内部思考荧光显微镜学和荧光分析动力学的跨关联的光谱学标注 ofGFP 的 BRI1。我们发现了那,响应 BR,有 AtFlot1-mCherry 的 BRI1-GFP 的合作本地化的度增加了,并且特别 BR 刺激了膜 BRI1 成为主观的联系 microdomain 的小径。我们也在 endocytosis 有缺点的 chc2-1 异种和 AtFlot1 amiRNA 15-5 线验证了这些观察。而且, bri1-EMS-suppressor 的 phosphorylation 地位的检查 BR 应答的基因表示的第 1a 测量表明膜 microdomains 影响 BR 发信号。这些结果 suggestthat BR 支持划分 BRI1 成功能的膜 microdomains 激活 BR 发信号。 | Li Wang Hong Li Xueqin Lv Tong Chen Ruili Li Yiqun xuei Jianjun Jiang Biao Jin Frantisek Baluska Jozef Samaj Xuelu Wang Jinxing Lin | 2015 | Molecular Plant2015，8，9： | 11 |
| 2 | The Subcellular Localization and Blue-Light- Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thalianaw显示文摘Phototropin 1 (phot1 ) 是为向光性,叶绿体运动,有气孔的洞,叶扩大,并且响应蓝光的太阳的追踪的一个光敏电阻器。有 PHOT1::GFP 的后面的更早的工作(Sakamoto 和布里格斯, 2002 ) ,我们调查了模式细胞并且在 34 d 的 phot1 的 subcellular 本地化 Arabidopsis thalinana 的旧变白的幼苗。是从本国的在上游的序列表示了, PHOT1::GFP 熔化蛋白质在子叶的离开轴心的纸巾并且在胚轴的伸长的区域强烈被表示。它中等在根顶附近在射击 / 根转变地区并且在房间被表示。一个荧光信号在根外皮,根帽子,和根是无法发现的顶端的分裂组织本身。子叶边缘附近的叶肉房间的血浆膜显得一致地标记但是跨墙由最近的房间部门创造了更强烈被标记。单个房间类型标记的模式与房间类型和发展舞台变化。蓝光的处理原因 PHOT1::GFP,均匀地相对开始在血浆膜散布了,与强烈标记的有小斑点的区域和完全缺乏在胚轴延伸区域在外皮的房间观察的 fluorescencea 现象最好的另外的区域重新组织变得进不同马赛克。伴随物与或追随者这个重组,进在所有房间类型的细胞质的 PHOT1::GFP 行动除了警卫房间调查了。它在黑暗在几个小时以后由未辩别出的机制从细胞质消失。它在细胞质的外观也不它在黑暗的最终的消失被翻译禁止者酮环己酰亚胺也不阻止,尽管后者过程被延迟。我们假设那 blue-light-induced phot1 重新本地化调制 blue-light-activated 信号 transduction。 | Ying-Lang Wan William Eisinger David Ehrhardt Ulrich Kubitscheck Frantisek Baluska Winslow Briggs | 2008 | Molecular Plant2008，1，1： | 10 |
| 3 | Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport显示文摘The most obvious symptom of Al toxicity is the inhibition of root growth. However, the mechanism of Al-inhibiting root growth remains to be elucidated. In this study, auxin transport and vesicle movement of an auxin-efflux carrier (PIN2) were investigated in Arabidopsis roots in response to Al stress. Results indicated that Al inhibited the apical transport of auxin in root tips of Arabidopsis significantly. The severe inhibition was localized in the cells of transition zone, where the concentration of auxin was only 34% that of the control. Brefeldin A (BFA), an inhibitor of vesicle transport, induced the dot-like structure of PIN2 vesicle significantly. Al decreased the size of dot-like structure of PIN2 vesicles. Re- sults of real-time RT-PCR and Western-blotting analysis showed that Al increased the transcript level of PIN2 and the accumulation of PIN2 protein in horizontal direction of plasma membrane, but decreased its distribution in endosomes, suggesting that Al inhibited the transport of PIN2 vesicles from plasma membrane to endosomes. Results of cytoskeleton-depolymering drugs indicated that it was via the pathway of disruption of actin microfilaments that Al inhibited the transport of PIN2 vesicles. Exposed to Al stress, the cells of elongation zone had less Al uptake and less transport frequency of vesicles than cells of transition zone. Taken together, our results suggested that Al inhibited root growth mainly by modulating the transport of PIN2 vesicles between plasma membrane and endosomes, thus block- ing auxin transport and root growth. | SHEN Hong HOU NingYan Markus SCHLICHT WAN YingLang Stefano MANCUSO Frantisek BALUSKA | 2008 | Chinese Science Bulletin2008，53，16： | 9 |
| 4 | 铝抑制拟南芥根尖PIN2循环和囊泡运输显示文摘铝对植物毒害作用最明显的症状是迅速抑制根尖生长.然而,铝抑制根尖生长的机制并不清楚.本文研究了铝对生长素和生长素运输载体(PIN2)囊泡运输的影响.结果表明,铝抑制拟南芥根尖生长素运输,其中过渡区生长素抑制率最高,达66%.布雷菲尔德菌素(Brefeldin A,BFA,一种囊泡运输抑制剂)明显诱导PIN2囊泡在细胞内形成点状结构,铝处理降低点状结构的大小,表明铝抑制PIN2囊泡在细胞内的运输.实时定量PCR和蛋白印迹反应发现,铝增加PIN2基因的转录表达,促进PIN2蛋白在细胞膜水平方向累积.细胞骨架解聚药物处理表明,铝抑制PIN2囊泡的运输,主要通过破坏肌球蛋白微丝来完成.铝处理下,拟南芥根尖伸长区细胞比过渡区具有较少的铝吸收和较低的囊泡运输频率.上述结果表明,通过调节生长素运输载体(PIN2)在质膜与胞内移动,阻碍生长素的运输,铝抑制了拟南芥根尖的生长. | 沈宏 侯凌艳 SCHLICHT Markus WAN Ying Lang MANCUSO Stefano BALUSKA Frantisek | 2008 | 科学通报2008，53，15： | 4 |
| 5 | Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains显示文摘Phototropin (辐透) 调停了发信号由蓝光(BL ) 开始了在在植物在血浆膜(下午) 优化光合的轻俘获起一个关键作用。然而,响应 BL 在 PM 位于辐透活动的规定下面的机制仍然保持大部分不清楚。在这研究,由单个粒子的追踪和 phot1-GFP 蛋白质的逐步的 photobleaching 分析,我们在黑暗 phot1 表明了那 ? 蛋白质留在一个不活跃的状态并且主要作为单体存在。Dimerization 和 phot1-GFP 的散开率响应 BL 以一种剂量依赖者方式增加了。相反, BL 没影响 kinase 不活跃的 phot1 D806N -GFP?but 确实提高了它的 dimerization,建议那 phot1 dimerization 独立于 phosphorylation。F ? rster 回声精力转移荧光一生成像显微镜学 ? 分析表明在 phot1-GFP 和充满甾醇的类脂化合物环境的一个标记之间的相互作用, AtRem1.3-mCherry,与 BL 处理的增加的时间被提高。然而,这个 BL 依赖的相互作用不在植物共同表示是明显的 phot1 D806N -GFP 和 AtRem1.3-mCherry,显示那 BL 便于功能的 phot1-GFP 的 translocation 进标记 AtRem1.3 的 microdomains 激活调停辐透的发信号。相反地,甾醇弄空稀释了 phot1-GFP 动力学, dimerization,和 phosphorylation。一起拿,这些结果显示膜 microdomains 充当在 PM 组织为激活的 phot1 的合适的功能必要的平台。 | Yiqun Xue Jingjing Xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin | 2018 | Molecular Plant2018，11，6： | 4 |
| 6 | Arabidopsis Synaptotagmin 2 Participates in Pollen Germination and Tube Growth and Is Delivered to Plasma Membrane via Conventional Secretion显示文摘Arabidopsis synaptotagmin (SYT2 ) 2 被报导了在体的房间参予一条异乎寻常的能分泌的小径。我们的结果证明 SYT2 主要在 Arabidopsis thaliana 的花粉被表示。展出的 syt2 T-DNA 和 RNA 干扰异种线的花粉减少了全部的萌芽并且阻碍了花粉试管生长。在 SYT2 对不同、补缀的分隔空间局部性,但是能与 Golgi 标记, BODIPY TR C5 ceramide 和 GmMan1-mCherry 共同本地化的花粉 tubeindicates 的 SYT2-GFP 熔化蛋白质的表示的分析。然而,对在 Arabidopsis 暂停房间的血浆膜局部性的 SYT2-DsRed-E5was,除了 Golgi 仪器。在血浆膜的 SYT2 的本地化被在花粉试管染色的 immunofluorescence 进一步支持。而且, brefeldin Atreatment 禁止了 SYT2 的运输到血浆膜并且引起了 SYT2 聚集并且形成扩大分隔空间。SYT2-C2AB 领域的截断也在 Golgi 仪器导致了 SYT2 的保留。phospholipid 有约束力的试金显示出的 invitro SYT2-C2AB 领域以一种钙依赖者方式绑在 phospholipid 膜。一起拿,我们的结果显示了那 SYT2 为花粉萌芽和花粉试管生长被要求,并且涉及常规 exocytosis。 | Hui Wang Shengcheng Han Wei Siao Chunqing Song Yun Xiang Xiaorong Wu Pengyu Cheng Hongjuan Li JAn Jasik Karol Micieta JAn Turna Boris Voigtt Frantisek Baluska Jin Liu Yingdian Wang Heping Zhao | 2015 | Molecular Plant2015，8，12： | 2 |
| 7 | Dynamics and roles of phragmoplast microfilaments in cell plate formation during cytokinesis of tobacco BY-2 cells显示文摘The phragmoplast is a special apparatus that functions in establishing a cell plate in dividing plant cells. It is known that microfilaments (MFs) are involved in constituting phragmoplast structure, but the dynamic distribution and role of phragmoplast MFs are far from being understood. In this study, the precise structure and dynamics of MFs during the initiation and the late lateral expansion of the phragmoplast were observed by using a tobacco BY-2 cell line stably expressing the microfilament reporter construct GFP-fABD2. Three-dimensional imaging showed that the phragmoplast MFs were initiated by two populations of MFs emerging between the reconstituting daughter nuclei at anaphase, which migrated to the mid-zone and gave rise to two layers of microfilament arrays. FM4-64 stained vesicles accumulated and fused with the cell plate between the two populations of MFs. The two layers of microfilament arrays of phragmoplast with ends overlapped always surrounded the centrifugally expanding cell plate. Partial disruption of MFs at metaphase by low concentration of latrunculin B resulted in the inhibition of the cell plate consolidation and the blockage of cell plate lateral expansion, whereas high concentration of latrunculin B restrained the progression of the cell cycle. Treating the cell after the initiation of phragmoplast led to the cease of the expansion of the cell plate. Our observations provide new insights into the precise structure and dynamics of phragmoplast MFs during cytokinesis and suggest that dynamic phragmoplast MFs are important in cell plate formation. | ZHANG Yan ZHANG WenJie BALUSKA Frantisek MENZEL Diedrik REN HaiYuni | 2009 | Chinese Science Bulletin2009，54，12： | 2 |
| 8 | Dynamic spatial reorganization of BSK1 complexes in the plasma membrane underpins signal-specific activation for growth and immunity显示文摘Growth and immunity are opposing processes that compete for cellular resources,and proper resource allocation is crucial for plant survival.BSK1 plays a key role in the regulation of both growth and immunity by associating with BRI1 and FLS2,respectively.However,it remains unclear how two antagonistic signals co-opt BSK1 to induce signal-specific activation.Here we show that the dynamic spatial reorganiization of BSK1 within the plasma membrane underlies the mechanism of signal-specific activation for growth or immunity.Resting BSK1 localizes to membrane rafts as complexes.Unlike BSK1-associated FLS2 and BRI1,flg22 or exogenous brassinosteroid(BR)treatment did not decrease BSK1 levels at the plasma membrane(PM)but rather induced BSK1 multimerization and dissociation from FLS2/BSK1 or BRI1/BSK1,respectively.Moreover,flg22-activated BSK1 translocated from membrane rafts to non-membrane-raft regions,whereas BR-activated BSK1 remained in membrane rafts.When applied together with flg22,BR suppressed various flg22-induced BSK1 activities such as BSK1 dissociation from FLS2/BSK1,BSK1 interaction with MAPKKK5,and BSK translocation together with MAPKKK5.Taken together,this study provides a unique insight into how the precise control of BSK1 spatiotemporal organization regulates the signaling specificity to balance plant growth and immunity. | Bodan Su Xi Zhang Li Li Sammar Abbas Meng Yu Yaning Cui Frantisek Baluska Inhwan Hwang Xiaoyi Shan Jinxing Lin | 2021 | Molecular Plant2021，14，4： | 2 |
| 9 | Eucaryotic cells and their cell bodies: celll theory revised显示文摘 | BALUSKA F VOLKMANN D BARLOW PW | 2004 | Ann Bot2004，94，1： | 1 |
| 10 | Endocytosis,actin cytoskeleton,and signaling显示文摘 | Samaj J Baluska F Voigt B | 2004 | Plant Physiol2004，135，3： | 1 |
| 11 | Endocytosis,actin cytoskeleton,and signaling显示文摘 | SAMAJ J BALUSKA F VOIGT B SCHLICHT M VOLKMANN D MENZEL D | 2004 | Plant Physiology2004，135，3： | 1 |
| 12 | Plant synapses: Actin-based adhesion domains for cell-to-cell communication显示文摘 | BALUSKA F VOLKMANN D MENZEL D | 2005 | Trend Plant Sci2005，10，： | 1 |
| 13 | Endocytosis actin cytoskeleton and signaling显示文摘 | Samaj J Baluska F Voigt B | 2004 | Pant physiol2004，135，3： | 1 |
| 14 | From signal to cell polarity:Mitogen-activated protein kinases as sensors and effectors of cytoskeleton dynamicity显示文摘 | Samai J Baluska F Hirt H | 2004 | J Exp Bot2004，55，395： | 1 |
| 15 | Latrunculin B-induced plant dwarfism:plant cell elongation is F-Actin-dependent 显示文摘 | Baluska F Jasik J Edelmann H G | 2001 | Developmental Biology2001，231，1： | 1 |
| 16 | Plant synapses:actin-based domains for cell-to-cell communication显示文摘 | Baluska F Volkmann D Menzel D | 2005 | Trends Plant Sci2005，10，3： | 1 |
| 17 | Actin cytoskeleton in plants: from transport network to signaling network显示文摘 | Volkmann D Baluska F | 1999 | Microscopy Research and Technique1999，47，： | 1 |
| 18 | The endocytic network in plants显示文摘 | Samaj J Read ND Volkmann D Menzel D Baluska F | | 0，，： | 1 |
| 19 | From signal to cell polarity: mitogen-activated protein kinases as sensors and effectors of cytoskeleton dynamicity显示文摘 | Samaj J Baluska F Hirt H | 2004 | J Exper Botany2004，395，55： | 1 |
| 20 | Extracellular matrix surface network of embryogenic units of friable maize callus contains arabinogalactan - proteins recognized by monoclonal antibody JIM4 显示文摘 | Samaj J Baluska F Bobak M | 1999 | Plant Cell Rep1999，18，： | 1 |
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