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您的检索式：作者名="SCHLICHT Markus"
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| 1 | Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport显示文摘The most obvious symptom of Al toxicity is the inhibition of root growth. However, the mechanism of Al-inhibiting root growth remains to be elucidated. In this study, auxin transport and vesicle movement of an auxin-efflux carrier (PIN2) were investigated in Arabidopsis roots in response to Al stress. Results indicated that Al inhibited the apical transport of auxin in root tips of Arabidopsis significantly. The severe inhibition was localized in the cells of transition zone, where the concentration of auxin was only 34% that of the control. Brefeldin A (BFA), an inhibitor of vesicle transport, induced the dot-like structure of PIN2 vesicle significantly. Al decreased the size of dot-like structure of PIN2 vesicles. Re- sults of real-time RT-PCR and Western-blotting analysis showed that Al increased the transcript level of PIN2 and the accumulation of PIN2 protein in horizontal direction of plasma membrane, but decreased its distribution in endosomes, suggesting that Al inhibited the transport of PIN2 vesicles from plasma membrane to endosomes. Results of cytoskeleton-depolymering drugs indicated that it was via the pathway of disruption of actin microfilaments that Al inhibited the transport of PIN2 vesicles. Exposed to Al stress, the cells of elongation zone had less Al uptake and less transport frequency of vesicles than cells of transition zone. Taken together, our results suggested that Al inhibited root growth mainly by modulating the transport of PIN2 vesicles between plasma membrane and endosomes, thus block- ing auxin transport and root growth. | SHEN Hong HOU NingYan Markus SCHLICHT WAN YingLang Stefano MANCUSO Frantisek BALUSKA | 2008 | Chinese Science Bulletin2008，53，16： | 9 |
| 2 | 铝抑制拟南芥根尖PIN2循环和囊泡运输显示文摘铝对植物毒害作用最明显的症状是迅速抑制根尖生长.然而,铝抑制根尖生长的机制并不清楚.本文研究了铝对生长素和生长素运输载体(PIN2)囊泡运输的影响.结果表明,铝抑制拟南芥根尖生长素运输,其中过渡区生长素抑制率最高,达66%.布雷菲尔德菌素(Brefeldin A,BFA,一种囊泡运输抑制剂)明显诱导PIN2囊泡在细胞内形成点状结构,铝处理降低点状结构的大小,表明铝抑制PIN2囊泡在细胞内的运输.实时定量PCR和蛋白印迹反应发现,铝增加PIN2基因的转录表达,促进PIN2蛋白在细胞膜水平方向累积.细胞骨架解聚药物处理表明,铝抑制PIN2囊泡的运输,主要通过破坏肌球蛋白微丝来完成.铝处理下,拟南芥根尖伸长区细胞比过渡区具有较少的铝吸收和较低的囊泡运输频率.上述结果表明,通过调节生长素运输载体(PIN2)在质膜与胞内移动,阻碍生长素的运输,铝抑制了拟南芥根尖的生长. | 沈宏 侯凌艳 SCHLICHT Markus WAN Ying Lang MANCUSO Stefano BALUSKA Frantisek | 2008 | 科学通报2008，53，15： | 4 |
| 3 | Both Local Anesthetics and Salbutamol Pretreatment Affect Reflex Bronchoconstriction in Volunteers with Asthma undergoing Awake Fiberoptic Intubation显示文摘 | Harald Groeben Markus Schlicht Sven Stieglitz Goran Pavlakovic Jürgen Peters | 2002 | Anesthesiology2002，，6： | 1 |
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