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您的检索式：作者名="B.Jacobson"
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| 1 | The ecological limits of hydrologic alteration (ELOHA): a new framework for developing regional environmental flow standards显示文摘 | N. LEROYPOFF BRIAN D.RICHTER ANGELA H.ARTHINGTON STUART E.BUNN ROBERT J.NAIMAN ELOISEKENDY MIKEACREMAN COLINAPSE BRIAN P.BLEDSOE MARY C.FREEMAN JAMESHENRIKSEN ROBERT B.JACOBSON JONATHAN G.KENNEN DAVID M.MERRITT JAY H.O’KEEFFE JULIAN D.OLDEN KEVINROGERS RE | 2009 | Freshwater Biology2009，，1： | 1 |
| 2 | Biodegradable nanoparticles with sustained release of functional siRNA in skin显示文摘 | Gunilla B.Jacobson EmilioGonzalez‐Gonzalez RyanSpitler RajeshShinde DevinLeake Roger L.Kaspar Christopher H.Contag Richard N.Zare | 2010 | J Pharm Sci2010，，10： | 1 |
| 3 | Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway显示文摘Metabolic engineering strategies have been successfully implemented to improve the production of isobutanol,a next-generation biofuel,in Saccharomyces cerevisiae.Here,we explore how two of these strategies,pathway re-localization and redox cofactor-balancing,affect the performance and physiology of isobutanol producing strains.We equipped yeast with isobutanol cassettes which had either a mitochondrial or cytosolic localized isobutanol pathway and used either a redox-imbalanced(NADPH-dependent)or redox-balanced(NADH-dependent)ketol-acid reductoisomerase enzyme.We then conducted transcriptomic,proteomic and metabolomic analyses to elucidate molecular differences between the engineered strains.Pathway localization had a large effect on isobutanol production with the strain expressing the mitochondrial-localized enzymes producing 3.8-fold more isobutanol than strains expressing the cytosolic enzymes.Cofactor-balancing did not improve isobutanol titers and instead the strain with the redox-imbalanced pathway produced 1.5-fold more isobutanol than the balanced version,albeit at low overall pathway flux.Functional genomic analyses suggested that the poor performances of the cytosolic pathway strains were in part due to a shortage in cytosolic Fe-S clusters,which are required cofactors for the dihydroxyacid dehydratase enzyme.We then demonstrated that this cofactor limitation may be partially recovered by disrupting iron homeostasis with a fra2 mutation,thereby increasing cellular iron levels.The resulting isobutanol titer of the fra2 null strain harboring a cytosolic-localized isobutanol pathway outperformed the strain with the mitochondrial-localized pathway by 1.3-fold,demonstrating that both localizations can support flux to isobutanol. | Francesca V.Gambacorta Ellen R.Wagner Tyler B.Jacobson Mary Tremaine Laura K.Muehlbauer Mick A.McGee Justin J.Baerwald Russell L.Wrobel John F.Wolters Mike Place Joshua J.Dietrich Dan Xie Jose Serate Shabda Gajbhiye Lisa Liu Maikayeng Vang-Smith Joshua J.Coon Yaoping Zhang Audrey P.Gasch Daniel Amador-Noguez Chris Todd Hittinger Trey K.Sato Brian F.Pfleger | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 4 | 网络新产品和升级产品的新闻发布显示文摘新产品Metricom公司把无线引向大规模应用 Metricom公司在其新的无线广域网中,提供了一种无线服务,该无线服务声称比其它公司更快更便宜。Ricochet微蜂窝数据网(MCDN)允许固定站的用户和便携计算机用户以及个人数字助手(PDA)的用户在本地或广域网上发送和接收图形文件或大数据文件。 Ricochet系统由用户无线调制解调器(其大小如同电视的遥控器)及被安装在大楼顶部或电话杆上的袖珍电台单元(其大小如同烤箱)组成。这些电台单元不同于传统无线网络, | Vicki B.Jacobson 魏红 | 1994 | 个人电脑1994，0，2： | 0 |
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