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您的检索式：作者名="Axel R. Zander"
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| 1 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 2 | Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients显示文摘 | William Krüger Cordula Krzizanowski Michael Holweg Marcus Stockschl?der Nicolaus Kr?ger Roman Jung Klaus Mross Walter Jonat Axel R. Zander | 1996 | Journal of Cancer Research and Clinical Oncology1996，，11： | 1 |
| 3 | Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use显示文摘 | Norbert Stute Katja Holtz Michael Bubenheim Claudia Lange Felix Blake Axel R. Zander | 2004 | Experimental Hematology2004，，12： | 1 |
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