|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells显示文摘瞄准:为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀,区分了 hepatocytic 房间。方法:MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF ), hepatocyte 生长因素( HGF ),表皮的生长因素( EGF ),和成纤维细胞生长因素 4 ( FGF-4 )独自一个,或在刚孤立的 FLC 的存在。在合作文化的房间被收获,并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ,白朊,和 alpha-fetoprotein 在不同房间人口被执行。结果:在指定文化条件下面,与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊, CK-18,和 AFP-RNA。在 wk 3, MSC 失去了 hepatocytic 基因表示,可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论:从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间,在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。 | Claudia Lange Helge Bruns Dietrich Kluth Axel R Zander Henning C Fiegel | 2006 | World Journal of Gastroenterology2006，12，15： | 23 |
| 2 | Nucleoporin 88 expression in hepatitis B and C virus-related liver diseases显示文摘AIM: To investigate the expression of nucleoporin 88 (Nup88) in hepatitis B virus (HBV) and C virus (HCV)-related liver diseases. METHODS: We generated a new monoclonal Nup88 antibody to investigate the Nup88 protein expression by immunohistochemistry (IHC) in 294 paraffin-embedded liver specimens comprising all stages of hepatocellular carcinogenesis. In addition, in cell culture experiments HBV-positive (HepG2.2.15 and HB611) and HBV-negative (HepG2) hepatoma cell lines were tested for the Nup88 expression by Western-immunoblotting to test data obtained by IHC.RESULTS: Specific Nup88 expression was found in chronic HCV hepatitis and unspecific chronic hepatitis, whereas no or very weak Nup88 expression was detected in normal liver. The Nup88 expression was markedly reduced or missing in mild chronic HBV infection and inversely correlated with HBcAg expression. Irrespective of the HBV- or HCV-status, increasing Nup88 expression was observed in cirrhosis and dysplastic nodules, and Nup88 was highly expressed in hepatocellular carcinomas. The intensity of Nup88 expression significantly increased during carcinogenesis (P < 0.0001) and correlated with dedifferentiation (P < 0.0001). Interestingly, Nup88 protein expression was significantly downregulated in HBV-positive HepG2.2.15 (P < 0.002) and HB611 (P < 0.001) cell lines as compared to HBV-negative HepG2 cells. CONCLUSION: Based on our immunohistochemical data, HBV and HCV are unlikely to influence the expression of Nup88 in cirrhotic and neoplastic liver tissue, but point to an interaction of HBV with the nuclear pore in chronic hepatitis. The expression of Nup88 in nonneoplastic liver tissue might reflect enhanced metabolic activity of the liver tissue. Our data strongly indicate a dichotomous role for Nup88 in non-neoplastic and neoplastic conditions of the liver. | Martina Knoess Anna Kordelia Kurz Olga Goreva Nuran Bektas Kai Breuhahn Magarethe Odenthal Peter Schirmacher Hans Peter Dienes C Thomas Bock Hanswalter Zentgraf Axel zur Hausen | 2006 | World Journal of Gastroenterology2006，12，36： | 4 |
| 3 | Paclitaxel inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using local drug delivery显示文摘 | Axel DI Kunert W Goggelmann C | 1997 | Circulation1997，96，2： | 1 |
| 4 | Comprehensive Identification of Proteins from MALDI Imaging显示文摘 | Stefan K. Maier Hannes Hahne Amin Moghaddas Gholami Benjamin Balluff Stephan Meding Cédrik Schoene Axel K. Walch Bernhard Kuster | 2013 | Molecular & Cellular Proteomics2013，，10： | 1 |
| 5 | Measurement of the specific surface area of 176 snow samples using methane adsorption at 77 K显示文摘 | Lot'c Legagneux Axel Cabanes Florent Domine | 2002 | Journal of Geophysical Research2002，107，17： | 1 |
| 6 | A short synthesis of 2,3-di ( hetero ) arylpyrido thiazepines 显示文摘 | AXEL C BRIC D PIERRE G | 1996 | Synthesis1996，，3： | 1 |
| 7 | Paclitaxel inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using local drug delivery显示文摘 | Axel DI Kunert W Goggelmann C | 1997 | Circulation1997，96，2： | 1 |
| 8 | Expression of candidate pheromone receptor genes in vomeronasal neurons显示文摘 | Dulac C Axel R | 1998 | Chem Senses1998，23，4： | 1 |
| 9 | A novel family of genes encoding putative pheromone receptors in mammals显示文摘 | Dulac C Axel R | 1995 | Cell1995，83，2： | 1 |
| 10 | Marine mammal Brucella isolates with different genomic characteristics dlsplay a differential response when infecting human macrophages in culture显示文摘 | Marianne M Michel S Z Axel C | 2009 | Microbes and Infection2009，11，： | 1 |
| 11 | Paclitaxel inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using local drug delivery显示文摘 | Axel DI Kunert W Goggelmann C | 1997 | Circulation1997，96，： | 1 |
| 12 | Quantification of cyclic dipeptides from cultures of Lactobacillus brevis R2Δ by HRGC/MS using stable isotope dilution assay显示文摘 | Axel C Zannini E Arendt EK | 2014 | Analytical and Bioanalytical Chemistry2014，406，910： | 1 |
| 13 | Fast multi-contrast MRI reconstruction显示文摘 | HUANG 1 CHEN C AXEL L | 2014 | Magnetic Resonance Imaging2014，32，10： | 1 |
| 14 | Fractures of the scapula: long- term results after conservative treatment 显示文摘 | Markus D Sehofer Axel C | 2009 | Arch Orthop Trauma Surg2009，129，11： | 1 |
| 15 | Multiple core-shell functionalized colloidal mesoporous silica nanoparticles显示文摘 | Valentina C Axel S Johann K | 2009 | J Am Chem Soc2009，131，11： | 1 |
| 16 | Paclitaxel in- hibits arterial smooth muscle cell proliferation and migra- tion in vitro and in vivo using local drag delivery 显示文摘 | Axel D I Kunert W Grogelman C | 1997 | Circulation1997，96，2： | 1 |
| 17 | Case study of hydraulic fracturing using finite element methods显示文摘 | NG AXEL K L SMALL J C | 1999 | Canadian Geotechnical Journal1999，36，5： | 1 |
| 18 | Paclitaxel inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using local drug delivery显示文摘 | Kunert W Goggelmann C | 1997 | Circulation1997，96，2： | 1 |
| 19 | Paclitaxel inhibits arterial smoothmusclecel proliferation andmigration in vitroand invivousing local drug delivery显示文摘 | Axel DI Kunert W Goggelmann C | | 0，，： | 1 |
| 20 | Paclitaxel inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using local drug delivery显示文摘 | Axel DI Kunert W Gggelmann C | 1997 | Circulation1997，96，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员