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| 1 | Extracellular vesicles in the diagnosis and treatment of central nervous system diseases显示文摘Extracellular vesicles,including exosomes and microvesicles,play a fundamental role in the activity of the nervous system,participating in signal transmission between neurons and providing the interaction of central nervous system with all body systems.In many neurodegenerative diseases,neurons pack toxic substances into vesicles and release them into the extracellular space,which leads to the spread of misfolded neurotoxic proteins.The contents of neuron-derived extracellular vesicles may indicate pathological changes in the central nervous system,and the analysis of extracellular vesicle molecular content contributes to the development of non-invasive methods for the diagnosis of many central nervous system diseases.Extracellular vesicles of neuronal origin can be isolated from various biological fluids due to their ability to cross the blood-brain barrier.Today,the diagnostic potential of almost all toxic proteins involved in nervous system disease pathogenesis,specificallyα-synuclein,tau protein,superoxide dismutase 1,FUS,leucine-rich repeat kinase 2,as well as some synaptic proteins,has been well evidenced.Special attention is paid to extracellular RNAs mostly associated with extracellular vesicles,which are important in the onset and development of many neurodegenerative diseases.Depending on parental cell type,extracellular vesicles may have different therapeutic properties,including neuroprotective,regenerative,and anti-inflammatory.Due to nano size,biosafety,ability to cross the blood-brain barrier,possibility of targeted delivery and the lack of an immune response,extracellular vesicles are a promising vehicle for the delivery of therapeutic substances for the treatment of neurodegenerative diseases and drug delivery to the brain.This review describes modern approaches of diagnosis and treatment of central nervous system diseases using extracellular vesicles. | Alisa A.Shaimardanova Valeriya V.Solovyeva Daria S.Chulpanova Victoria James Kristina V.Kitaeva Albert A.Rizvanov | 2020 | Neural Regeneration Research2020，15，4： | 7 |
| 2 | Functionality of a bicistronic construction containing HEXA and HEXB genes encoding β-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses显示文摘Tay-Sachs disease and Sandhoff disease are severe hereditary neurodegenerative disorders caused by a deficiency ofβ-hexosaminidase A(HexA)enzyme,which results in the accumulation of GM2 gangliosides in the nervous system cells.In this work,we analyzed the efficacy and safety of cell-mediated gene therapy for Sandhoff disease and Sandhoff disease using a bicistronic lentiviral vector encoding cDNA of HexAα-andβ-subunit genes separated by the nucleotide sequence of a P2A peptide(HEXA-HEXB).The functionality of the bicistronic construct containing the HEXA-HEXB genetic cassette was analyzed in a culture of HEK293T cells and human umbilical cord blood mononuclear cells(hUCBMCs).Our results showed that the enzymatic activity of HexA in the conditioned medium harvested from genetically modified HEK293T-HEXA-HEXB and hUCBMCs-HEXA-HEXB was increased by 23 and 8 times,respectively,compared with the conditioned medium of native cells.Western blot analysis showed that hUCBMCs-HEXA-HEXB secreted both completely separated HEXA and HEXB proteins,and an uncleaved protein containing HEXA+HEXB linked by the P2A peptide.Intravenous injection of genetically modified hUCBMCs-HEXA-HEXB to laboratory Wistar rats was carried out,and the HexA enzymatic activity in the blood plasma of experimental animals,as well as the number of live cells of immune system organs(spleen,thymus,bone marrow,lymph nodes)were determined.A significant increase in the enzymatic activity of HexA in the blood plasma of laboratory rats on days 6 and 9(by 2.5 and 3 times,respectively)after the administration of hUCBMCsHEXA-HEXB was shown.At the same time,the number of live cells in the studied organs remained unchanged.Thus,the functionality of the bicistronic genetic construct encoding cDNA of the HEXA and HEXB genes separated by the nucleotide sequence of the P2A peptide was shown in vitro and in vivo.We hypothesize that due to the natural ability of hUCBMCs to overcome biological barriers,such a strategy can restore the activity of the missing enzyme in the central nervous system of patients with GM2 gangliosidoses.Based on the obtained data,it can be concluded that intravenous administration of hUCBMCs with HexA overexpression is a promising method of the therapy for GM2 gangliosidoses.The animal protocol was approved by the Animal Ethics Committee of the Kazan Federal University(No.23)on June 30,2020. | Alisa A.Shaimardanova Daria S.Chulpanova Valeriya V.Solovyeva Aleksandr M.Aimaletdinov Albert A.Rizvanov | 2022 | Neural Regeneration Research2022，17，1： | 3 |
| 3 | Androgen synthesis inhibitors in the treatment of castration-resistant prostate cancer显示文摘gonadal 睾丸激素合成的抑制首先代表标准为变形前列腺癌症的治疗的线治疗。在得阉割抵抗的前列腺癌症(CRPC ) 的病人的多数,然而,通过自己在肾上腺或在肿瘤以内生产的雄激素检测雄激素受体(AR ) 的坚持的激活是可能的。Abiraterone 醋酸盐作为 17 α 与活动作为双功能的细胞色素 P450 酶 CYP17 的一个不可逆的禁止者被开发; -hydroxylase 和 17,20-lyase。CYP17 为从胆固醇的 nongonadal 雄激素的生产是必要的。基于与 abiraterone 和泼尼松对泼尼松在全面幸存(OS ) 显示出重要改进的阶段 III 试用,在 2011 的 abiraterone 的规章的赞同代表了指向 AR 为与 CRPC 在人改进结果是必要的原则的证明。 17 α 的抑制;由 abiraterone 的 -hydroxylase 由于垂体的调停皮质醇的抑制的损失导致在上游的 mineralocorticoids 的累积促肾上腺皮质的荷尔蒙( ACTH ),为 17,20-lyase 为 CYP17 禁止者的开发向一个基本原理提供增加的特性( orteronel , galeterone 和 VT-464 )没有外长的 corticosteroids ,那能潜在地被管理。在这篇文章,我们考察 abiraterone 和另外的 CYP17 禁止者的发展;有 abiraterone 的最近的研究在 quality-of-life,反应的潜在的早预言者,并且关于另外的代理人的 abiraterone 的最佳的定序上通知我们的理解象药效果那样的临床的参数;并且提供卓见进抵抗机制给导致临床的试用,药联合设计了延长 abiraterone 利益或恢复 abiraterone 活动的 CYP17 禁止者的翻译研究结果。 | Mark N Stein | 2014 | Asian Journal of Andrology2014，16，3： | 3 |
| 4 | 汞离子选择电极在海水痕量元素检测中的应用显示文摘由于自由汞离子(II)在低浓度时不易保持其形态,在此以汞络合平衡的基本原理为基础,计算并讨论了汞在溶液体系中的存在形态,从而获得稳定的低浓度自由汞离子以及溶液中自由汞离子浓度和总汞浓度之间的关系.通过对汞离子选择电极(Hg-ISE)在类似海水的缓冲溶液体系实验测试及分析,其结果显示在自由汞离子浓度高于10-19mol/L时,总汞浓度和自由汞浓度仍然保持成一定的线性关系,表明可以通过检测自由汞离子浓度来检测海水中总汞浓度. | 胡卫军 邹绍芳 Alisa Rudnitskaya 王平 | 2007 | 传感技术学报2007，20，6： | 2 |
| 5 | Impaired swallowing mechanics of post radiation therapy head and neck cancer patients: A retrospective videofluoroscopic study显示文摘AIM: To determine swallowing outcomes and hyolaryngeal mechanics associated with post radiation therapy head and neck cancer(rt HNC) patients using videofluoroscopic swallow studies. METHODS: In this retrospective cohort study, video-fluoroscopic images of rt HNC patients(n = 21) were compared with age and gender matched controls(n = 21). Penetration-aspiration of the bolus and bolus residue were measured as swallowing outcome variables. Timing and displacement measurements of the anterior and posterior muscular slings elevating the hyolaryngeal complex were acquired. Coordinate data of anatomical landmarks mapping the action of the anterior muscles(suprahyoid muscles) and posterior muscles(long pharyngeal muscles) were used to calculate the distance measurements, and slice numbers were used to calculate time intervals. Canonical variate analysis with post-hoc discriminant function analysis was performed on coordinate data to determine multivariate mechanics of swallowing associated with treatment. Pharyngeal constriction ratio(PCR) was also measured to determine if weak pharyngeal constriction is associated with post radiation therapy.RESULTS: The rt HNC group was characterized by poor swallowing outcomes compared to the control group in regards to: Penetration-aspiration scale(P < 0.0001), normalized residue ratio scale(NRRS) for the valleculae(P = 0.002) and NRRS for the piriform sinuses(P = 0.003). Timing and distance measurements of the anterior muscular sling were not significantly different in the two groups, whereas for the PMS time of displacement was abbreviated(P = 0.002) and distance of excursion was reduced(P = 0.02) in the rt HNC group. A canonical variate analysis shows a significant reduction in pharyngeal mechanics in the rt HNC group(P < 0.0001). The PCR was significantly higher in the test group than the control group(P = 0.0001) indicating reduced efficiency in pharyngeal clearance. CONCLUSION: Using videofluoroscopy, this study shows rt HNC patients have worse swallowing outcomes associated with reduced hyolaryngeal mechanics and pharyngeal constriction compared with controls. | William G Pearson Jr Alisa A Davidoff Zachary M Smith Dorothy E Adams Susan E Langmore | 2016 | World Journal of Radiology2016，8，2： | 2 |
| 6 | Evaluation of spatial and temporal patterns of insect damage and aflatoxin level in the pre-harvest corn fields to improve management tactics显示文摘在与有一致基因背景的植物的一个玉米领域里的黄麴毒素污染的关系的昆虫损坏的空间、时间的模式很好没被理解。在昆虫损坏和黄麴毒素在的空间模式的以前的考试以后预先收获玉米地,我们进一步与二采样检验了喂圆块、喂核的昆虫损坏,和黄麴毒素水平的空间、时间的模式在在 2008 和 2009 预先收获。由每 ear/kernel-feeding 昆虫的喂的损坏(即,丝绸 / 圆块上的玉米一种有害幼虫 / 秋天粟蚕损坏,和玉米核由的褪色坏透臭虫) 并且玉米象鼻虫人口用五只耳朵在每个格子点被估计。采样数据在两年里处于昆虫损坏和黄麴毒素污染显示出域边效果。玉米象鼻虫比任何一个玉米一种有害幼虫更经常向聚集的分布趋于或坏透两年里的臭虫损坏。为黄麴毒素水平检测聚集的分发的频率是昆虫损坏评价的不到任何东西。坏透臭虫损坏和玉米象鼻虫数字更仔细比是玉米一种有害幼虫的与黄麴毒素水平被联系损坏。另外,空间时间的协会(χ
) 的索引证明玉米象鼻虫的数字被联系在之间第一(4 个星期预先收获) 并且秒(1 个星期预先收获) 所有地上的两年里的采样。相反,在来自 Belflower 农场上的地的第一和第二采样之间的玉米一种有害幼虫损坏,和黄麴毒素水平和玉米一种有害幼虫在 Lang 农场上从这块地损坏在 2009 被分裂。 | Xinzhi Ni Jeffrey P. Wilson Michael D. Toews G. David Buntin R. Dewey Lee Xin Li Zhongren Lei Kanglai He Wenwei Xu Xianchun Li Alisa Huffaker Eric A. Schmelz | 2014 | Insect Science2014，21，5： | 2 |
| 7 | Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda显示文摘The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas. | Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang | 2022 | Protein & Cell2022，13，7： | 2 |
| 8 | 激动人心的丙肝治疗革命显示文摘最新公布的III期临床数据显示,telaprevir——首个治疗丙肝的蛋白酶抑制剂,显著提高了丙肝的治愈率,给丙肝药物研发带来了美好的前景。 | Alisa Opar 朱宝益 | 2010 | 中国处方药2010，，7： | 2 |
| 9 | A Systematic Review of Medial and Lateral Entry Pinning Versus Lateral Entry Pinning for Supracondylar Fractures of the Humerus显示文摘 | Carmen Alisa Brauer Ben Minsuk Lee Donald S. Bae Peter M. Waters Mininder S. Kocher | 2007 | Journal of Pediatric Orthopaedics2007，，2： | 2 |
| 10 | 滇池水中细菌和古菌氮代谢功能基因的空间分布显示文摘氮代谢在滇池水生态系统氮素循环和转化过程中起到重要的作用,不仅真核生物参与氮素转化,原核生物作为氮素循环的主要驱动者,在氮素生物化学循环中的作用更不容忽视.基于16S rDNA高通量测序技术,监测滇池草海和外海区域13个点位,分析滇池水中原核生物氮循环功能关键基因的分布特征.结果发现,滇池水中细菌35门,427属,主要以变形菌门和拟杆菌门为优势门类;古菌14门,61属,主要以广古菌门为优势门类;β多样性指数显示滇池整体细菌丰富度指数高于古菌,草海细菌多样性指数高于外海.PICRUSt功能解析表明细菌和古菌具有功能上的丰富性,细菌中有35个参与氮代谢的KO通路,涉及氮异化硝酸盐还原基因nirB、一氧化氮还原酶基因norB和硝酸还原酶基因nasK等关键基因;古菌中有23个参与氮代谢的KO通路,涉及固氮酶基因nifH、nifK和nifD,古菌固氮酶基因拷贝数显著高于其它氮代谢基因,草海中古菌氮代谢能力整体高于外海,滇池水中古菌比细菌固氮潜能更大.本研究从原核生物氮循环中功能基因的角度,探讨滇池不同区域水中细菌和古菌氮循环差异,为进一步揭示氮循环机制,解决氮素污染引起的富营养化提供理论参考. | 张宇 左剑恶 王丝可 Alisa Salimova 李爱军 李玲玲 | 2020 | 环境科学2020，41，6： | 2 |
| 11 | Unmasking of α-fetoprotein-specific CD4+ T cell responses in hepatocellular carcinoma patients undergoing embolization 显示文摘 | AYARU L PEREIRA SP ALISA A | 2007 | J Immunol2007，178，3： | 1 |
| 12 | Human CD4+ T cells recognize an epitope within α-fetoprotein sequence and develop into TGF-β-producing CD4+ T cells 显示文摘 | ALISA A BOSWELL S PATHAN AA | 2008 | J Immunol2008，180，7： | 1 |
| 13 | Mind- fulness- based stress reduction compared with cognitive behav- ioral therapy for the treatment of insomnia comorbid with canc- er: A randomized, partially blinded, noninferiority trial显示文摘 | Sheila N Garland Linda E Carlson Alisa J Stephens | 2014 | Jour- nal of Clinical Oncology2014，32，5： | 1 |
| 14 | Extracorporeal liver support with molecular adsorbents recirculating system in patients with severe acute alcoholic hepatitis显示文摘 | Rajiv Jalan Sambit Sen Christian Steiner Dharmesh Kapoor Akeel Alisa Roger Williams | 2002 | Journal of Hepatology2002，，1： | 1 |
| 15 | Human CD4 (+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4 (+) T cells显示文摘 | Alisa A Boswell S Pathan AA | 2008 | J Immunol2008，180，7： | 1 |
| 16 | Alpha-fetoprotein impairs APC function and induces their apoptosis显示文摘 | Um SH Mulhall C Alisa A | 2004 | J Immunol2004，173，3： | 1 |
| 17 | a-Fetoprotein Impairs APC Function and Induces Their Apoptosis显示文摘 | Um S H MulhaU C Alisa A | 2004 | J lmmunol2004，173，3： | 1 |
| 18 | Instrumental measurement of beer taste attributes using an electronic tongue显示文摘 | Alisa Rudnitskaya Evgeny Polshin Dmitry Kirsanov | 2009 | Analytica Chimica Acta2009，646，12： | 1 |
| 19 | Expansion of anti-AFP Th1 and Tcl responses in hepatocellular carcinoma occur in different stages of disease显示文摘 | Behboudi S Alisa A Boswell S et a1 | 2010 | Br J Cancer2010，102，4： | 1 |
| 20 | Tasting of beverages using an electronic tongue显示文摘 | Andrey Legin Alisa Rudnitskaya Yuri Vlasov Corrado Di Natale Fabrizio Davide Arnaldo D’Amico | 1997 | Sensors & Actuators: B Chemical1997，，1： | 1 |
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