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| 1 | Sensitive and rapid determination of amantadine without derivatization in human plasma by LC–MS/MS for a bioequivalence study显示文摘A highly sensitive, rapid and rugged liquid chromatography-tandem mass spectrometry(LC-ESI-MS/MS)method was developed for reliable estimation of amantadine(AMD), an antiviral drug in human plasma.The analyte and internal standard(IS), amantadine-d6(AMD-d6), were extracted from 200 m L plasma by solid phase extraction on Phenomenex Strata-X-C 33 m cartridges. Chromatography was performed on Synergi? Hydro-RP C18(150 mm ? 4.6 mm, 4 mm) analytical column using a mixture of acetonitrile and10 m M ammonium formate, p H 3.0(80:20, v/v) as the mobile phase. Detection and quantitation was done by multiple reaction monitoring in the positive ionization mode for AMD(m/z 152.1-135.1) and IS(m/z 158.0-141.1) on a triple quadrupole mass spectrometer. The assay was linear in the concentration range of 0.50–500 ng/m L with correlation coefficient(r2) Z 0.9969. The limit of detection of the method was 0.18 ng/m L. The intra-batch and inter-batch precisions were r 5.42% and the accuracy varied from98.47% to 105.72%. The extraction recovery of amantadine was precise and quantitative in the range of97.89%–100.28%. IS-normalized matrix factors for amantadine varied from 0.981 to 1.012. The stability of AMD in whole blood and plasma was evaluated under different conditions. The developed method was successfully applied for a bioequivalence study with 100 mg of AMD in 32 healthy volunteers. The reproducibility of the assay was determined by reanalysis of 134 subject samples. | Abhaysingh Bhadoriya Shivprakash Rathnam Bhavesh DasANDi Dharmesh Parmar Mallika Sanyal Pranav S.Shrivastav | 2018 | Journal of Pharmaceutical Analysis2018，8，3： | 9 |
| 2 | A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity显示文摘AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management. | BM Srikanta MN Siddaraju Shylaja M Dharmesh | 2007 | World Journal of Gastroenterology2007，13，39： | 6 |
| 3 | Esophageal tuberculosis presenting with hematemesis显示文摘Esophageal tuberculosis is rare, constituting about 0.3% of gastrointestinal tuberculosis. It presents commonly with dysphagia, cough, chest pain in addition to fever and weight loss. Complications may include hemorrhage from the lesion, development of arterioesophageal fistula, esophagocutaneous fistula or tracheoesophageal fistula. There are very few reports of esophageal tuberculosis presenting with hematemesis due to ulceration. We report a patient with hematemesis that was due to the erosion of tuberculous subcarinal lymph nodes into the esophagus. A 15-year-old boy presented with hemetemesis as his only complaint. Esophagogastroduodenoscopy(EGD) revealed an eccentric ulcerative lesion involving 50% of circumference of the esophagus. Biopsy showed caseating epitheloid granulomas with lymphocytic infiltrates suggestive of tuberculosis. Computerised tomography of the thorax revealed thickening of the mid-esophagus with enlarged mediastinal lymph nodes in the subcarinal region compressing the esophagus along with moderate right sided pleural effusion. Patient was treated with anti-tuberculosis therapy(Rifampicin, Isoniazid, Pyrazinamide, Ethambutol) for 6 mo. Repeat EGD showed scarring and mucosal tags with complete resolution of the esophageal ulcer. | Samit S Jain Piyush O Somani Rajeshkumar C Mahey Dharmesh K Shah Qais Q Contractor Pravin M Rathi | 2013 | World Journal of Gastrointestinal Endoscopy2013，5，11： | 6 |
| 4 | Quantitation of tadalafil in human plasma using a sensitive and rapid LC-MS/MS method for a bioequivalence study显示文摘A highly sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed for the determination of tadalafil(TAD) in human plasma. TAD and its deuterated internal standard(IS), tadalafil-d3, were extracted from 200 mL plasma using Phenomenex Strata-X-C 33 m extraction cartridges. Chromatographic analysis was carried out on Synergi? Hydro-RP C18(100 mm ? 4.6 mm, 4 mm)column with a mobile phase consisting of methanol and 10 m M ammonium formate, p H 4.0(90:10, v/v),delivered at a flow rate of 0.9 m L/min. Quantitation of the protonated analyte was done on a triple quadrupole mass spectrometer using multiple reaction monitoring via electrospray ionization. The precursor to product ions transitions monitored for TAD and TAD-d3 were m/z 390.3-268.2 and m/z393.1-271.2, respectively. The calibration curve was linear over the concentration range of 0.50–500 ng/m L with correlation coefficient, r2 Z 0.9994. Acceptable intra-batch and inter-batch precision(r3.7%) and accuracy(97.8% to 104.1%) were obtained at five concentration levels. The recovery of TAD from spiked plasma was highly precise and quantitative(98.95% to 100.61%). Further, the effect of endogenous matrix components was minimal. TAD was found to be stable under different storage conditions in human plasma and also in whole blood samples. The validated method was successfully used to determine TAD plasma concentration in a bioequivalence study with 20 mg TAD tablets in 24 healthy volunteers. Method performance was evaluated by reanalyzing 115 study samples. | Abhaysingh Bhadoriya Bhavesh Dasandi Dharmesh Parmar Priyanka A. Shah Pranav S. Shrivastav | 2018 | Journal of Pharmaceutical Analysis2018，8，4： | 4 |
| 5 | Inhibition of gastric H ^+ , K^+ - ATPase and Helicobacter pylori growth by phenolic antioxidants of curcuma amada显示文摘 | Siddaraju MN Dharmesh SM | 2007 | J Agri Food Chem2007，55，18： | 1 |
| 6 | Extracorporeal liver support with molecular adsorbents recirculating system in patients with severe acute alcoholic hepatitis显示文摘 | Rajiv Jalan Sambit Sen Christian Steiner Dharmesh Kapoor Akeel Alisa Roger Williams | 2002 | Journal of Hepatology2002，，1： | 1 |
| 7 | Inhibition of gastric H^+ , K^+ - ATPasc and Helieobacter priori growth by phenolic antioxidants of zingiber officinale 显示文摘 | Siddaraju MN Dharmesh SM | 2007 | Mol Nutr Food Res2007，51，3： | 1 |
| 8 | Oeh'entide as therapculie op- tion for congenital idiopathic ehylothorax:a ease series显示文摘 | Shall Dharmesh Sinn John KH | 2012 | Acta Paediatr2012，101，4： | 1 |
| 9 | Gastroprotective effect of swallow root (Decalepis hamiltonii) extract:possible involvement of H+-K+-ATPase inhibition and antioxidative mechanism显示文摘 | Naik Y Jayaram S Harish Nayaka MA Lakshman Dharmesh SM | | 0，，1： | 1 |
| 10 | Characterization and theoretical analysis of protein fouling of cellulose acetate membrane during constant flux dead-end microfiltration显示文摘 | Xinghua Sun Dharmesh M. Kanani Raja Ghosh | 2008 | Journal of Membrane Science2008，，1： | 1 |
| 11 | Comparison of total knee arthroplasty in stiff knees and knees with good preoperative range of motion 显示文摘 | Khatri A Dharmesh B Mathotra C | 2009 | J Knee Surg2009，22，： | 1 |
| 12 | Inhibition of Helicobacter pylori growth and its cytotoxicity by 2-hydroxy 4-methoxy benzaldehyde of Decalepis hamiltonii (Wight & Arn); a new functional attribute显示文摘 | Belagihalli M. Srikanta Mysore A. Harish Nayaka Shylaja M. Dharmesh | 2011 | Biochimie2011，，4： | 1 |
| 13 | Inhibition of Aldose Reductase from CataractedEye Lenses by Finger Millet(Eleusine Coracana)Polyphenols显示文摘 | Chethan S Dharmesh Shylaja M Malleshi NagappaG | 2008 | Bioorganic&Medicinal Chemis-try2008，16，10： | 1 |
| 14 | Correlation be-tween near-infrared spectroscopy and magnetic resonance imaging of rat brain oxygenation modulation显示文摘 | Chen Y Dharmesh R T Intes X | 2003 | Phys Medic Biol2003，48，4： | 1 |
| 15 | Nuclear protein binding at the β-myosin heavy chain A/T-rich element Is enriched following increased skeletal muscle activity 显示文摘 | Dharmesh RV John JM Richard WT | 1999 | J Biol Chem1999，274，30： | 1 |
| 16 | Antioxidant and cytotoxic effect of oxidized lutein in human cervical carcinoma cells (HeLa)显示文摘 | Rangaswamy Lakshminarayana Upparahalli V. Sathish Shylaja M. Dharmesh Vallikannan Baskaran | 2010 | Food and Chemical Toxicology2010，，7： | 1 |
| 17 | Monoclonal anti-DNA antibodies: structure,specificity,and biology显示文摘 | Meera RK Dharmesh DD | 1997 | Methods: A companion to Methods in Enzymology1997，11，： | 1 |
| 18 | Reversible and irreversible membrane fouling during in-line microfiltration of concentrated protein solutions显示文摘 | Dharmesh M.Kanani Xinghua Sun Raja Ghosh | | 0，，1： | 1 |
| 19 | Ulcer preventive and antioxidative properties of astaxanthin from Haematococcus pluvialis显示文摘 | Kamath B S Srikanta B M Dharmesh S M | 2008 | Eur J Pharmacol2008，590，13： | 1 |
| 20 | Estrogen modulates expression of the glycosyltransferases that synthesize sulfated oligosaccharides on lutropin 显示文摘 | Dharmesh SM Baenziger JU | 1993 | Proc Natl Acad Sci U S A1993，190，11： | 1 |
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