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| 1 | Glutamate reduces experimental intestinal hyperpermeability and facilitates glutamine support of gut integrity显示文摘AIM:To assess whether glutamate plays a similar role to glutamine in preserving gut wall integrity.METHODS:The effects of glutamine and glutamate on induced hyperpermeability in intestinal cell lines were studied.Paracellular hyperpermeability was induced in Caco2.BBE and HT-29CL.19A cell lines by adding phorbol-12,13-dibutyrate(PDB) apically,after which the effects of glutamine and glutamate on horseradish peroxidase(HRP) diffusion were studied.An inhibitor of glutamate transport(L-trans-pyrrolidine-2,4-dicarboxylic acid:trans-PDC) and an irreversible blocker(acivicin) of the extracellular glutamine to glutamate converting enzyme,γ-glutamyltransferase,were used.RESULTS:Apical to basolateral HRP flux increased significantly compared to controls not exposed to PDB (n=30,P<0.001).Glutamine application reduced hyperpermeability by 19%and 39%in the respective cell lines.Glutamate application reduced hyperpermeability by 30%and 20%,respectively.Incubation of HT29CL.19A cells with acivicin and subsequent PDB and glutamine addition increased permeability levels.Incubation of Caco2.BBE cells with trans-PDC followed by PDB and glutamate addition also resulted in high permeability levels.CONCLUSION:Apical glutamate-similar to glutaminecan decrease induced paracellular hyperpermeability.Extracellular conversion of glutamine to glutamate and subsequent uptake of glutamate could be a pivotal step in the mechanism underlying the protective effect of glutamine. | Mechteld AR Vermeulen Jeffrey de Jong Mathijs J Vaessen Paul AM van Leeuwen Alexander PJ Houdijk | 2011 | World Journal of Gastroenterology2011，17，12： | 9 |
| 2 | Antioxidant enriched enteral nutrition and oxidative stress after major gastrointestinal tract surgery显示文摘AIM: To investigate the effects of an enteral supple-ment containing antioxidants on circulating levels of antioxidants and indicators of oxidative stress after major gastrointestinal surgery.METHODS: Twenty-one patients undergoing major upper gastrointestinal tract surgery were randomised in a single centre, open label study on the effect of postoperative enteral nutrition supplementedwith antioxidants. The effect on circulating levels of antioxidants and indicators of oxidative stress, such as F2-isoprostane, was studied. RESULTS: The antioxidant enteral supplement showed no adverse effects and was well tolerated. After surgery a decrease in the circulating levels of antioxidant parameters was observed. Only selenium and glutamine levels were restored to pre-operative values one week after surgery. F2-isoprostane increased in the first three postoperative days only in the antioxidant supplemented group. Lipopolysaccharide binding protein (LBP) levels decreased faster in the antioxidant group after surgery.CONCLUSION: Despite lower antioxidant levels there was no increase in the circulating markers of oxidative stress on the first day after major abdominal surgery. The rise in F2-isoprostane in patients receiving the antioxidant supplement may be related to the conversion of antioxidants to oxidants which raises questions on antioxidant supplementation. Module AOX restored the postoperative decrease in selenium levels. The rapid decrease in LBP levels in the antioxidant group suggests a possible protective effect on gut wall integrity. Further studies are needed on the role of oxidative stress on outcome and the use of antioxidants in patients undergoing major abdominal surgery. | Mireille FM van Stijn Gerdien C Ligthart-Melis Petra G Boelens Peter G Scheffer Tom Teerlink Jos WR Twisk Alexander PJ Houdijk Paul AM van Leeuwen | 2008 | World Journal of Gastroenterology2008，14，45： | 4 |
| 3 | In situ liver transection with portal vein ligation for rapid growth of the future liver remnant in two‐stage liver resection显示文摘 | W. T. Knoefel I. Gabor A. Rehders A. Alexander M. Krausch J. Schulte am Esch G. Fürst S. A. Topp | 2012 | Br J Surg2012，，3： | 2 |
| 4 | Indoleamine 2,3-dioxygenase expression in transplanted NOD islets prolongs gratt snrvlvat aster adoptive transter of diabetogenic splenocytes显示文摘 | Crawford M Bertera S | 2002 | Diabetes2002，51，： | 1 |
| 5 | Acute kidney injury post neonatal asphyxia显示文摘 | Durkan AM Alexander RT | 2011 | J Pediatr2011，158，2： | 1 |
| 6 | Expression and tar- geting of CX3CL1 (fractalkine) in renal tubular epithelial cells 显示文摘 | Durkan AM Alexander RT Liu GY | 2007 | J Am Soc Nephrol2007，18，1： | 1 |
| 7 | Acute kidney injury post neonatal as?phyxia显示文摘 | Durkan AM Alexander RT | 2011 | J Pediatr2011，158，2： | 1 |
| 8 | Genotypic vatiations in the accumulation of Cd, Cu, Pb and Zn exhibited by six commonly grown vegetables显示文摘 | Alexander PD Alloway B J Dourado AM | 2006 | Environ- mental Pollution2006，144，： | 1 |
| 9 | The role of negative intraarticular pressure and the long head of biceps tendon on passive stability of the glenohumeral joint 显示文摘 | Alexander S Southgate DF Bull AM | 2013 | J Shoulder Elbow Surg2013，22，1： | 1 |
| 10 | Indoleamine 2,3- dioxygenase expression in transplanted NOD islets prolongs graft survival after adoptive Transfer of diabetogenie splenocytes 显示文摘 | Alexander AM Crawford M Bertera S | 2002 | Diabetes2002，51，2： | 1 |
| 11 | Non-operative management of herniated nucleus pulposus:patient selection by the extension sign显示文摘 | Alexander AH Jones AM Rosenbaum DH | 1991 | Orthop Trans1991，15，： | 1 |
| 12 | The effect of surface roughness on the stress adaptation of trabecular architecture around a cylindrica limplant显示文摘 | Luo G Sadegh AM Alexander H | 1999 | J Biomech1999，32，3： | 1 |
| 13 | Prevalence,pathophysiology,and treatment of patients with asthma and gastroesophageal reflux disease显示文摘 | Alexander JA Hunt LW Patel AM | | 0，，： | 1 |
| 14 | Evaluation of the Cepheid Xpert MTB/RIF assay 显示文摘 | SHINNICK TM STARKS AM ALEXANDER HL | 2015 | Expert Rev Mol Diagn2015，15，1： | 1 |
| 15 | Acute kidney injury post neonatal as- phyxia显示文摘 | Durken AM Alexander RT | 2011 | J Pediatr2011，158，2： | 1 |
| 16 | Indoleamine 2,3- Dioxygenase expression in transplanted NOD islets prolongs graft survival after adoptive transfer of diabetogenic splenocytes显示文摘 | Alexander AM Crawford M Bertera M | 2002 | Diabetes2002，51，12： | 1 |
| 17 | Effects of gamma irradiation on the transduction of dividing and nondividing cells in brain and muscle of rats by adeno-associated virus vectors显示文摘 | Alexander IE Russell DW Spence AM | 1996 | Human Gene Ther1996，7，： | 1 |
| 18 | Expression and targeting ofCX3CL1 (fractalkine) in renal tubular epithelial cells显示文摘 | Durkan AM Alexander RT Liu GY ef al | 2007 | J Am SocNephrol2007，18，1： | 1 |
| 19 | Elec- trocardiogram screening for disorders that cause sudden cardiac death in asymptomatic children : a meta-analysis 显示文摘 | RODDAY AM TRIEDMAN JK ALEXANDER ME | 2012 | Pediatrics2012，129，4： | 1 |
| 20 | A biomechanical comparison of single and double-row fixation in arthroscopic rotator cuff repair显示文摘 | Smith CD Alexander S Hill AM | 2006 | J Bone Joint Surg Am2006，88，11： | 1 |
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