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| 1 | Antimicrobial activity of Sapindus mukorossi and Rheum emodi extracts against H pylori: In vitro and in vivo studies显示文摘AIM: To evaluate the antibacterial activity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi). METHODS: Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylori, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram +ve (S. aureus, B. subtilis) and two Gram -ve (E. coli, P. vugaris) organisms were screened for their susceptibility patterns against these extracts. RESULTS: In our screening, all 30 resistant isolates and the other four organisms (two Gram +ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris) were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited H pylori at very low concentrations. In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations (10 μg/mL) for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi (2.5 mg/mL) and R. emodi (3.0 mg/mL) given orally for seven days. CONCLUSION: We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages. | Mohammed Ibrahim Aleem A Khan Santosh K Tiwari Mohammed Aejaz Habeeb MN Khaja CM Habibullah | 2006 | World Journal of Gastroenterology2006，12，44： | 46 |
| 2 | Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies显示文摘AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats. METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats. RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities. CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury. | Mohammed Ibrahim Mohammed Nane Khaja Anjum Aara Aleem Ahmed Khan Mohammed Aejaz Habeeb Yalavarthy Prameela Devi Mangamoori Lakshmi Narasu Chitoor Mohammed Habibullah | 2008 | World Journal of Gastroenterology2008，14，16： | 24 |
| 3 | 基于热模拟实验的富有机质泥页岩成岩作用及演化特征显示文摘富有机质泥页岩蕴含丰富的油气资源,但成岩研究基础薄弱,已成为制约页岩油气勘探开发进程的重要因素。通过开展成岩热模拟实验,结合扫描电镜观察、流体成分测试及有机酸测试等实验测试手段,全面描述实验过程中所发生的水—岩化学反应,试图揭示泥页岩成岩过程及成岩演化规律。研究结果显示,有机质热演化过程中存在一个较宽的有机酸生成窗口,对孔隙流体性质具有重要影响;长石和方解石存在接力溶蚀现象,长石溶蚀高峰过后紧接着出现方解石溶蚀高峰,但方解石溶蚀窗较窄,此后出现方解石重新沉淀结晶;黏土矿物转化及长石的溶蚀过程中会产生大量自生微晶石英,成链状或簇状胶结泥页岩骨架。泥页岩地层作为一个相对封闭体系,各类成岩作用之间相互关联,相互影响,构成错综复杂的成岩体系,进一步增加其成岩作用研究难度。 | 栾国强 董春梅 马存飞 林承焰 张津营 吕夏霏 Muhammad Aleem Zahid | 2016 | 沉积学报2016，34，6： | 18 |
| 4 | Characterization of hepatic progenitors from human fetal liver during second trimester显示文摘AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule (EpCAM) as a marker from human fetal liver and investigate the expression of human leukocyte antigen (HLA) and their markers associated with hepatic progenitor cells. METHODS: EpCAM +ve cells were isolated using magnetic cell sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 (integrin β1), CD49f (integrin α6) and CD90 (Thy 1) was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ (A, B, C) and class Ⅱ (DR) expression was studied by flow cytometry only. RESULTS: FACS analysis indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA class Ⅰ, albumin and AFP but negative for HLA class Ⅱ (DR) and CD45. RT PCR showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical staining, EpCAM +ve cells were shown positive signals for CK18 and albumin. CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor. | Mekala Subba Rao Aleem Ahmed Khan Nyamath Parveen Mohammed Aejaz Habeeb Chittoor Mohammed Habibullah Gopal Pande | 2008 | World Journal of Gastroenterology2008，14，37： | 16 |
| 5 | Association between cag-pathogenicity island in Helicobacter pylori isolates from peptic ulcer,gastric carcinoma,and non-ulcer dyspepsia subjects with histological changes显示文摘AIM: To investigate the presence of the cay-pathogenicity island and the associated histological damage caused by strains with complete cay-PAI and with partial deletions in correlation to the disease status.METHODS: We analyzed the complete cag-PAI of 174representative Helicobacter pylori (H pylori ) clinical isolates obtained from patients with duodenal ulcer,gastric ulcer, gastric cancer, and non-ulcer dyspepsia using eight different oligonucleotide primers viz cagA1,cagA2, cagAP1, cagAP2, cagE, cagT, LEC-1, LEC-2spanning five different loci of the whole cag-PAI by polymerase chain reaction (PCR).RESULTS: The complete screening of the genes comprising the cag-PAI showed that larger proportions of subjects with gastric ulcer (97.8%) inhabited strains with complete cag-PAI, followed by gastric cancer (85.7%),non-ulcer dyspepsia (7.1%), and duodenal ulcer (6.9%),significant differences were found in the percentagedistribution of the genes in all the clinical groups studied.It was found that strains with complete cag-PAI were able to cause severe histological damage than with the partially deleted ones.CONCLUSION: The cay-PAI is a strong virulent marker in the disease pathogenesis as it is shown that a large number of those infected with strain with complete cag-PAI had one or the other of the irreversible gastric pathologies and interestingly 18.5% of them developed gastric carcinoma. The presence of an intact cayPAI correlates with the development of more severe pathology, and such strains were found more frequently in patients with severe gastroduodenal disease. Partial deletions of the cag-PAI appear to be sufficient to render the organism less pathogenic. | Mahaboob Ali Aleem A Khan Santosh K Tiwari Niyaz Ahmed L Venkateswar Rao CM Habibullah | 2005 | World Journal of Gastroenterology2005，11，43： | 15 |
| 6 | Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker显示文摘AIM: To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. METHODS: Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation. RESULTS: Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture. CONCLUSION: The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations. | Parveen Nyamath Ayesha Alvi Aejaz Habeeb Sanjeev Khosla Aleem A Khan CM Habibullah | 2007 | World Journal of Gastroenterology2007，13，16： | 9 |
| 7 | Hepatic stem cells: A viable approach for the treatment of liver cirrhosis显示文摘Liver cirrhosis is characterized by distortion of liver architecture, necrosis of hepatocytes and regenerative nodules formation leading to cirrhosis. Various types of cell sources have been used for the management and treatment of decompensated liver cirrhosis.Knowledge of stem cells has offered a new dimension for regenerative therapy and has been considered as one of the potential adjuvant treatment modality in patients with end stage liver diseases(ESLD). Human fetal hepatic progenitor cells are less immunogenic than adult ones. They are highly propagative and challenging to cryopreservation. In our earlier studies we have demonstrated that fetuses at 10-18 wk of gestation age contain a large number of actively dividing hepatic stem and progenitor cells which possess bipotent nature having potential to differentiate into bile duct cells and mature hepatocytes. Hepatic stem cell therapy for the treatment of ESLD is in their early stage of the translation. The emerging technology of decellularization and recellularization might offer a significant platform for developing bioengineered personalized livers to come over the scarcity of desired number of donor organs for the treatment of ESLD. Despite these significant advancements long-term tracking of stem cells in human is the most important subject nowadays in order to answer several unsettles issues regarding the route of delivery, the choice of stem cell type(s), the cell number and the timepoint of cell delivery for the treatment in a chronic setting. Answering to these questions will further contribute to the development of safer, noninvasive, and repeatable imaging modalities that could discover better cell therapeutic approaches from bench to bedside. Combinatorial approach of decellularization and nanotechnology could pave a way towards the better understanding in determination of cell fate posttransplantation. | Md Aejaz Habeeb Sandeep Kumar Vishwakarma Avinash Bardia Aleem Ahmed Khan | 2015 | World Journal of Stem Cells2015，7，5： | 5 |
| 8 | Cardiac papillary fibroelastoma显示文摘Papillary fibroelastoma(PFE)is a primary,histologically benign endocardial neoplasm.Though PFE has long been reported as the second most common primary cardiac neoplasm,it has since pulled ahead of cardiac myxomas,largely due to evolving cardiac imaging modalities.While PFEs are benign histologically,they have the potential for devastating clinical consequences,transient ischemic attack,stroke,myocardial infarction,syncope,pulmonary,and peripheral embolism.Despite increased detection rate,there remains uncertainty regarding etiology,exact prevalence,and clinical management of PFEs.This paucity of information is reflected by the lack of official guidelines on this matter.In this article,we aim to summarize the current state of understanding regarding PFE and discuss areas of ongoing controversy. | Dominika M Zoltowska Edin Sadic Kyeesha Becoats Shreya Ghetiya Aleem Azal Ali Srinivasan Sattiraju Emil Missov | 2021 | Journal of Geriatric Cardiology2021，18，5： | 4 |
| 9 | Subversion of cellular autophagy during virus infection:Insights from hepatitis B and hepatitis C viruses显示文摘Autophagy is a self-eating process,in which the damaged or excessed cell organelles and misfolded protein aggregates are removed from the cellular microenvironment.Autophagy is generally thought of as a pro-survival mechanism which is not only important for balancing energy supply at times of nutrient deprivation but also in the removal of various stress stimuli to ensure homeostasis.In addition to the target materials of“self”origin,autophagy can also eliminate intracellular pathogens and acts as a defense mechanism to curb infections.In addition,autophagy is linked to the host cell's innate immune response.However,viruses have evolved various strategies to manipulate and overtake host cell machinery to establish productive replication and maintain infectious process.In fact,replication of many viruses has been found to be autophagy-dependent and suppression of autophagy can potentially affect the viral replication.Thus,autophagy can either serve as an anti-viral defense mechanism or a pro-viral process that supports viral replication.Hepatitis B virus(HBV)and hepatitis C virus(HCV)are known to co-opt cellular autophagy process as a pro-viral tool.Both viruses also induce mitophagy,which contributes to the establishment of chronic hepatitis.This review focuses on the roles of autophagy and mitophagy in the chronic liver disease pathogenesis associated with HBV and HCV infections. | Mohsin Khan Hasan Imam Aleem Siddiqui | 2018 | Liver Research2018，2，3： | 4 |
| 10 | 丙型肝炎病毒非结构蛋白5a对细胞周期的影响显示文摘丙型肝炎病毒(HCV)感染是慢性肝炎、肝硬化的主要病因之一,并与肝细胞癌的发生关系非常密切.但HCV导致原发性肝细胞癌的机制,特别是其基因产物(结构蛋白和非结构蛋白)在致癌中的作用仍不清楚.HCV非结构蛋白5A(NS5A)能激活多种转录因子,并与细胞内因子结合,影响其功能,导致细胞增殖分化异常,可能与原发性肝细胞癌的发生有关.为进一步探讨它们之间的联系,采用放线菌素D(Act.D)作用于HepG2肝癌细胞株研究HCV NS5A对细胞周期调控的影响,从而为阐明HCV参与肝细胞癌的分子机制积累更多的资料. | 龚国忠 Aleem Siddiqui 苏先狮 | 2004 | 中华肝脏病杂志2004，12，1： | 3 |
| 11 | Pneumomediastinum, pneumorachis, subcutaneous emphysema: An unusual complication of leukemia in a child显示文摘Pneumorrhachis(PR), or epidural emphysema, denotes the presence of air in the spinal epidural space. It can be associated with a variety of etiologies, including trauma; recent iatrogenic manipulations during surgical, anesthesiological and diagnostic interventions;malignancy and its associated therapy. It usually represents an asymptomatic epiphenomenon but also can be symptomatic by itself, as well as by its underlying pathology, and rarely can be fatal. The pathogenesis and etiology of PR are varied and can sometimes be a diagnostic challenge. As such, there are no standard guidelines for the management of symptomatic PR and its treatment is often individualized. Here, we present a case of a 14-year-old boy treated for leukemia who developed this complication and whether chemotherapy related or not, it proved to be fatal for him. To our knowledge, this is the first case in the literature of this complication with acute lymphoblastic leukemia. | Hakim Irfan Showkat Aleem Jan Arif Hussain Sarmast Gull Mohammad Bhat Basharat Mujtaba Jan Yasir Bashir | 2013 | World Journal of Clinical Cases2013，1，7： | 2 |
| 12 | Multicentered prospective investigator initiated study to evaluate the clinical outcomes with extracorporeal cytokine adsorption device (CytoSorb®) in patients with sepsis and septic shock显示文摘BACKGROUND Sepsis is a severe clinical syndrome related to the host response to infection.The severity of infections is due to an activation cascade that will lead to an auto amplifying cytokine production:The cytokine storm.Hemoadsorption by CytoSorb®therapy is a new technology that helps to address the cytokine storm and to regain control over various inflammatory conditions.AIM To evaluate prospectively CytoSorb®therapy used as an adjunctive therapy along with standard of care in septic patients admitted to intensive care unit(ICU).METHODS This was a prospective,real time,investigator initiated,observational multicenter study conducted in patients admitted to the ICU with sepsis and septic shock.The improvement of mean arterial pressure and reduction of vasopressor needs were evaluated as primary outcome.The change in laboratory parameters,sepsis scores[acute physiology and chronic health evaluation(APACHE II)and sequential organ failure assessment(SOFA)]and vital parameters were considered as secondary outcome.The outcomes were also evaluated in the survivor and nonsurvivor group.Descriptive statistics were used;a P value<0.05 was considered RESULTS Overall,45 patients aged≥18 and≤80 years were included;the majority were men(n=31;69.0%),with mean age 47.16±14.11 years.Post CytoSorb®therapy,26 patients survived and 3 patients were lost to follow-up.In the survivor group,the percentage dose reduction in vasopressor was norepinephrine(51.4%),epinephrine(69.4%)and vasopressin(13.9%).A reduction in interleukin-6 levels(52.3%)was observed in the survivor group.Platelet count improved to 30.1%(P=0.2938),and total lung capacity count significantly reduced by 33%(P<0.0001).Serum creatinine and serum lactate were reduced by 33.3%(P=0.0190)and 39.4%(P=0.0120),respectively.The mean APACHE II score was 25.46±2.91 and SOFA scores was 12.90±4.02 before initiation of CytoSorb®therapy,and they were reduced significantly post therapy(APACHE II 20.1±2.47;P<0.0001 and SOFA 9.04±3.00;P=0.0003)in the survivor group.The predicted mortality in our patient population before CytoSorb®therapy was 56.5%,and it was reduced to 48.8%(actual mortality)after CytoSorb®therapy.We reported 75%survival rate in patients given treatment in<24 h of ICU admission and 68%survival rates in patients given treatment within 24-48 h of ICU admission.In the survivor group,the average number of days spent in the ICU was 4.44±1.66 d;while in the nonsurvivor group,the average number of days spent in ICU was 8.5±15.9 d.CytoSorb®therapy was safe and well tolerated with no adverse events reported.CONCLUSION CytoSorb®might be an effective adjuvant therapy in stabilizing sepsis and septic shock patients.However,it is advisable to start the therapy at an early stage(preferably within 24 h after onset of septic shock). | Rajib Paul Prachee Sathe Senthil Kumar Shiva Prasad Ma Aleem Prashant Sakhalvalkar | 2021 | World Journal of Critical Care Medicine2021，10，1： | 2 |
| 13 | Helicobacter pylori and other Helicobacter species DNA in human bile samples from patients with various hepato-biliary diseases显示文摘瞄准:为了由 16S rRNA 基因的嵌套的 PCR 调查 Helicobacter 种类的存在,由 Helicobacter pylori (H pylori ) 的存在跟随了 16S rRNA,脲,在获得在的胆汁的 cagA 基因内视镜后退从 60 印第安人的 cholangio-pancreatography (ERCP ) 使遭到。方法:六十件胆汁样品从在 ERCP 与各种各样的 hepato 胆汁的疾病和控制题目诊断的病人被获得。PCR 分析为 Helicobacter 类 16S rRNA 基因和 H pylori (16S rRNA,脲和 cagA ) 用教材被执行基因。胃的 H pylori 地位也从与各种各样的 hepato 胆汁的疾病和控制从病人在内视镜检查法获得的活体检视被估计。控制组主要与胃的混乱由题目组成了。定序分析被执行证实有 16S rRNA 和 cagA 教材的 PCR 产品从 H pylori 被导出。结果没有 Helicobacters 从胆汁样品在文化被种。Helicobacter DNA 在 96.7% 组和 6.6% 的胆汁被检测我和 II 分别地。十来自组,我为 16S rRNA 和脲是积极的, 9 为 cagA 是积极的基因。在从控制的 2 的对比, 1 与 16S 放大了教材使用了的 rRNA,脲和 cagA。16S rRNA 基因和 cagA 的序列是 99% 类似于 Helicobacter pylori。结论:Helicobacters 与各种各样的 hepato 胆汁的混乱的致病被联系。 | Santosh K Tiwari Aleem A Khan Mohd Ibrahim Mohd Aejaz Habeeb C M Habibullah | 2006 | World Journal of Gastroenterology2006，12，14： | 2 |
| 14 | Frank hematuria as the presentation feature of acute leukemia显示文摘 | Suriya OM Aleem A | | 0，，05： | 1 |
| 15 | Laser welding of low carbon steel and thermal stress analysis 显示文摘 | Yilbas B S Arif A F M Abdul Aleem B J | 2010 | Optics & Laser Technology2010，42，5： | 1 |
| 16 | Macrophage migration inhibitory factor, Toll-like receptor 4, and CD14 polymorphisms with altered expression levels in patients with ulcerative colitis显示文摘 | Gunisetty Sivaram Santosh K. Tiwari Avinash Bardia Farha Anjum Satti Vishnupriya Aejaz Habeeb Aleem A. Khan | 2012 | Human Immunology2012，，2： | 1 |
| 17 | World Health Organi- zation randomized trial of calcium supplementation among low calcium intake pregnant women 显示文摘 | Villar J Abdel - Aleem H Merialdi M | 2006 | Am J Obstet Gynecol2006，194，3： | 1 |
| 18 | Functional polymorphisms in XRCC - 1 and APE - 1 contribute to increased apoptosis and risk of ulcerative colitis显示文摘 | Avinash Bardia Santosh K. Tiwari Sivaram Gunisetty Farha Anjum Pratibha Nallari Md. Aejaz Habeeb Aleem A. Khan | 2012 | Inflammation Research2012，，4： | 1 |
| 19 | Imperfect Information, Screening, and the Costs of Informal Lending: A Study of a Rural Credit Market in Pakistan显示文摘 | Aleem I | 1990 | The World Bank Economic Review1990，，4： | 1 |
| 20 | World Health Organization randomized trial of calcium supplementation a- mong low calcium intake pregnant women显示文摘 | VILLAR J ABDEL ALEEM H MERIALDI M | 2006 | Am J Obstet Gyne- col2006，194，3： | 1 |
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