|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries显示文摘Due to the limitations in autogenous nerve grafting or Schwann cell transplantation,large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit.Cell based therapies provide a novel treatment for peripheral nerve injuries.In this study,we first experimented an optimal scaffold material synthesis protocol,from where we selected the 10%GFD formula(10%GelMA hydrogel,recombinant human basic fibroblast growth factor and dental pulp stem cells(DPSCs))to fill a cellulose/soy protein isolate composite membrane(CSM)tube to construct a third generation of nerve regeneration conduit,CSM-GFD.Then this CSM-GFD conduit was applied to repair a 15-mm long defect of sciatic nerve in a rat model.After 12 week post implant surgery,at histologic level,we found CSM-GFD conduit could regenerate nerve tissue like neuron and Schwann like nerve cells and myelinated nerve fibers.At physical level,CSM-GFD achieved functional recovery assessed by a sciatic functional index study.In both levels,CSM-GFD performed like what gold standard,the nerve autograft,could do.Further,we unveiled that almost all newly formed nerve tissue at defect site was originated from the direct differentiation of exogeneous DPSCs in CSM-GFD.In conclusion,we claimed that this third-generation nerve regeneration conduit,CSM-GFD,could be a promising tissue engineering approach to replace the conventional nerve autograft to treat the large gap defect in peripheral nerve injuries. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2021 | Bioactive Materials2021，6，3： | 11 |
| 2 | An updatc on antifungual targets and mechanisms of resistance in Candida albicans 显示文摘 | Akons RA | 2005 | Med Mycol2005，43，4： | 1 |
| 3 | Elevatedlevels of homocysteine increase IL-6 production inmonocytic cells显示文摘 | Van Akon BE Jansen J Deventer SJ | 2000 | Blood Coagul Fibrinoiysis2000，，11： | 1 |
| 4 | 3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities显示文摘Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility. | Tzu-Cheng Sung Chao-Wen Heish Henry Hsin-Chung Lee Jhe-Yu Hsu Chun-Ko Wang Jia-Hua Wang Yu-Ru Zhu Shih-Hsi Jen Shih-Tien Hsu Abdurahman HHirad Abdullah AAlarfaj Akon Higuchi | 2021 | Journal of Materials Science & Technology2021，，4： | 1 |
| 5 | Chemically modified polysulfone hollow fibers with vinyl pyrrolidone having improved blood compatibility 显示文摘 | Akon H Kazunobu S | 2002 | Biomaterials2002，23，： | 1 |
| 6 | 显示文摘 | Akon | 2002 | Biomaterials2002，23，13： | 1 |
| 7 | Chiral separation of phenylalaine by ultrafiltration through immobilized DNA membranes显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，： | 1 |
| 8 | 显示文摘 | | 2003 | Biomaterials2003，24，19： | 1 |
| 9 | Chemically modified polysulfone hollow fibers with vinylpyrrolidone having improved blood compatibility 显示文摘 | Akon H Kazunobu S Masaharu H | 2002 | Biomaterials2002，23，： | 1 |
| 10 | Collaborative Mobile Target Imaging in UWB Wireless Radar Sensor Networks显示文摘 | Arik M Akon O.B | | 0，，06： | 1 |
| 11 | DSC investigation of the states of water in poly (vinyl2 alcohol) membranes显示文摘 | AKON H TOSHIR L | 1985 | Polymer1985，26，8： | 1 |
| 12 | Chemically mod- ified polysulfone hollow fibers with vinylpyrrolidone having improved blood compatibility 显示文摘 | AKON H KAZUNOBU S MASAHARU H | 2002 | Biomaterials2002，23，13： | 1 |
| 13 | Pre-operative vessel mapping and early post-operative surveillance duplex scanning of arteriovenous fistulae 显示文摘 | Ives C I Akon J A George J | 2009 | Vase Access2009，10，1： | 1 |
| 14 | Biofouling-resistance control of expanded poly(tetrafluoroethylene) membrane via atmospheric plasma-induced surface PEGylation显示文摘 | Antoine Venault Yung Chang Hung-Hsing Hsu Jheng-Fong Jhong Hui-Shan Yang Ta-Chin Wei Kuo-Lun Tung Akon Higuchi James Huang | 2013 | Journal of Membrane Science2013，，： | 1 |
| 15 | Morbidity of overweight(≥85th percentile)in the first 2 years of life显示文摘 | Shibli R Rubin L Akons H | | 0，，02： | 1 |
| 16 | Chemically modified polysulfone hollow fibers with vinylpyrrolidone having improved blood compatibility 显示文摘 | Akon H Kazunobu S Masaharu H | 2002 | Biomaterials2002，23，： | 1 |
| 17 | 显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，： | 1 |
| 18 | Chemically modified polusulfone hollow fibers with vinylpyrrolidone having improved blood compatibility 显示文摘 | AKON H KAZUNOBU S MASAHARU H | 2002 | Biomaterials2002，23，13： | 1 |
| 19 | Paleoproterozoic granitoids in the basement of Bangladesh:a piece of the Indian shield or an exotic fragment of the Gondwana jigsaw显示文摘 | Ameen S.M.M Wilde S.A Kabir M.Z Akon E Chowdhury K.R Khan M.S.H | | 0，，： | 1 |
| 20 | DSC investigation of the states of water in poly(vinyl2 alcohol)membranes显示文摘 | AKON H TOSHIR L | 1985 | Polymer1985，26，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员