|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries显示文摘Due to the limitations in autogenous nerve grafting or Schwann cell transplantation,large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit.Cell based therapies provide a novel treatment for peripheral nerve injuries.In this study,we first experimented an optimal scaffold material synthesis protocol,from where we selected the 10%GFD formula(10%GelMA hydrogel,recombinant human basic fibroblast growth factor and dental pulp stem cells(DPSCs))to fill a cellulose/soy protein isolate composite membrane(CSM)tube to construct a third generation of nerve regeneration conduit,CSM-GFD.Then this CSM-GFD conduit was applied to repair a 15-mm long defect of sciatic nerve in a rat model.After 12 week post implant surgery,at histologic level,we found CSM-GFD conduit could regenerate nerve tissue like neuron and Schwann like nerve cells and myelinated nerve fibers.At physical level,CSM-GFD achieved functional recovery assessed by a sciatic functional index study.In both levels,CSM-GFD performed like what gold standard,the nerve autograft,could do.Further,we unveiled that almost all newly formed nerve tissue at defect site was originated from the direct differentiation of exogeneous DPSCs in CSM-GFD.In conclusion,we claimed that this third-generation nerve regeneration conduit,CSM-GFD,could be a promising tissue engineering approach to replace the conventional nerve autograft to treat the large gap defect in peripheral nerve injuries. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2021 | Bioactive Materials2021，6，3： | 11 |
| 2 | 3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities显示文摘Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility. | Tzu-Cheng Sung Chao-Wen Heish Henry Hsin-Chung Lee Jhe-Yu Hsu Chun-Ko Wang Jia-Hua Wang Yu-Ru Zhu Shih-Hsi Jen Shih-Tien Hsu Abdurahman HHirad Abdullah AAlarfaj Akon Higuchi | 2021 | Journal of Materials Science & Technology2021，，4： | 1 |
| 3 | 显示文摘 | Akon | 2002 | Biomaterials2002，23，13： | 1 |
| 4 | Chiral separation of phenylalaine by ultrafiltration through immobilized DNA membranes显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，： | 1 |
| 5 | 显示文摘 | | 2003 | Biomaterials2003，24，19： | 1 |
| 6 | Biofouling-resistance control of expanded poly(tetrafluoroethylene) membrane via atmospheric plasma-induced surface PEGylation显示文摘 | Antoine Venault Yung Chang Hung-Hsing Hsu Jheng-Fong Jhong Hui-Shan Yang Ta-Chin Wei Kuo-Lun Tung Akon Higuchi James Huang | 2013 | Journal of Membrane Science2013，，： | 1 |
| 7 | 显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，： | 1 |
| 8 | Optical resolution of amino acids by ultrafiltration membranes containing serum albumin显示文摘 | Akon Higuchi Mariko Hara Toru Horiuchi | 1994 | Journal of Membrane Science1994，93，： | 1 |
| 9 | Optical resolution of amino acid by ultrafiltration through immobilized DNA membranes显示文摘 | Akon Higuchi Kentaro Furuta Hiroshi Yomogita | 2002 | Desalination2002，148，13： | 1 |
| 10 | Optical resolution of amino acid by ultrafiltration using recognition sites of DNA显示文摘 | Akon Higuchi Hiroshi Yomogita Boo Ok Yoon | 2002 | Journal of Membrane Science2002，205，12： | 1 |
| 11 | Chiral separation of phenylalanine by ultrafiltration through immobilized DNA membranes显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，12： | 1 |
| 12 | Optical resolution of amino acid by ultrafiltration using recognition sites of DNA 显示文摘 | Akon Higuchi H Y Toshinori Kojima Mariko Hara | 2002 | J Membr Sci2002，205，： | 1 |
| 13 | Chiral separation of phenylalanine by ultrafiltration through immobilized DNA membranes 显示文摘 | Akon Higuchi Yuichiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，： | 1 |
| 14 | Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods显示文摘 | Akon Higuchi Shigeo Tamiya Taro Tsubomura Akira Katoh Chong-Su Cho Toshihiro Akaike Mariko Hara | 2000 | Journal of Biomaterials Science Polymer Edition2000，，2： | 1 |
| 15 | Optical resolution of amino acid by ultrafiltration using recognition sites of DNA 显示文摘 | Akon Higuchi Hiroshi Yomogita Boo Ok Yoon | 2002 | Journal of Membrane Science2002，205，12： | 1 |
| 16 | Chiral separation of phenylalanine by ultrafiltration through immobilized DNA membranes显示文摘 | Akon Higuchi Yuiehiro Higuchi Kentaro Furuta | 2003 | Journal of Membrane Science2003，221，12： | 1 |
| 17 | Corrigendum to‘Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries’[Bioactive Mater.6(3)(2021)638-654]显示文摘The authors regret that the author(A.H.)was removed as an agreement could not be reached between the two affiliations of the author.The authors would like to apologise for any inconvenience caused. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2022 | Bioactive Materials2022，7，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员