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| 1 | 内毒素诱导大鼠葡萄膜炎的组织平片和切片研究显示文摘目的:探讨内毒素诱导的视网膜脉络膜改变和平片、切片结果的差异。方法:从内毒素注射的Lewis鼠制备视网膜和脉络膜-巩膜复合体平片以及眼组织冰冻切片,并进行免疫组织化学染色。结果:平片上显示,注射后4小时单核细胞浸润,随后,大量的单核巨噬细胞浸润至整个视网膜和脉络膜,脉络膜中尚有MHC-Ⅱ类抗原阳性细胞增多;切片上发现,于注射后一定时期,视网膜和脉络膜中有上述细胞浸润。结论:平片技术为视网膜脉络膜原发性严重受累提供了确凿的证据,内毒素诱导的葡萄膜炎可作为人类全葡萄膜炎的动物模型。 | 杨培增 Alex F. de Vos Lidy Broersma Aize kijlstra | 1996 | 中华眼底病杂志1996，12，1： | 16 |
| 2 | TLR4-MyD88在大鼠急性前葡萄膜炎虹膜中的表达显示文摘目的观察内毒素诱导的大鼠急性前葡萄膜炎(EIU)虹膜组织内Toll样受体4(TLR4)、髓样分化因子88(MyD88)及核因子-κBp65(NF-κB p65)的表达。方法Wistar大鼠50只,随机分为5组,0、12、24、48、72h,每组10只,0h组为正常对照组,其余4组均足垫部注射霍乱弧菌内毒素脂多糖(LPS)200μg,建立EIU动物模型,每隔2h用裂隙灯观察大鼠眼前节炎症反应。通过铺片免疫组织化学染色,检测虹膜睫状体组织内TLR4、MyD88和NF-κBp65的表达,并对虹膜内TLR4+和MyD88+及NF-κBp65+细胞进行计数。结果注射后24~48h大鼠眼前段的炎症反应达到高峰,72h炎症反应逐渐缓解。组织病理学检查表明,虹膜睫状体组织的炎性细胞浸润在24~48h达到高峰,与临床反应结果相符。TLR4在模型鼠虹膜睫状体炎复合体中表达的免疫组织化学检测结果表明,0h组虹膜铺片内无阳性细胞,12h后可见细胞形态大多为类圆形的阳性细胞,48h达高峰,72h阳性细胞数开始减少,各组阳性细胞数总体差异有统计学意义(F=46.79,P<0.05)。MyD88和NF-κBp65的表达与TLR4的改变趋势相一致(F=54.37,P<0.05;F=85.32,P<0.05)。结论内毒素诱导的EIU虹膜内,TLR4及其下游信号传导分子的表达量发生改变,提示TLR4-MyD88依赖传导途径可能参与了EIU的发病。 | 李上 卢弘 胡小凤 陈巍 杨培增 Aize Kijlstra 许颖知 王婧 | 2010 | 眼科研究2010，28，2： | 10 |
| 3 | 实验性自身免疫性葡萄膜视网膜炎中浸润细胞的表型及其凋亡的研究显示文摘目的 探讨实验性自身免疫性葡萄膜视网膜炎 (experimental autoimmune uveoretinitis,EAU)中参与的细胞表型及其凋亡。 方法 用光感受器间维生素 A类结合蛋白 (interphotoreceptorretinoid- binding protein,IRBP)免疫 16只 L ewis鼠后 ,于眼组织切片和平片上进行免疫组织化学染色和原位凋亡染色 ,所用抗体为抗单核细胞、巨噬细胞 (EDI)、MHC- 类抗原 (OX6 )、T淋巴细胞 (R73)的单克隆抗体 ,所用原位凋亡试剂盒为 TACS1Klenow。 结果 用 IRBP免疫 L ewis鼠后 ,16只鼠中 12只发生了临床可见的葡萄膜炎 ,炎症平均得分为 1.2 9± 0 .7级 ;免疫组织化学染色发现葡萄膜和视网膜中有大量的单核细胞、淋巴细胞及 MHC- +细胞浸润 ,这些组织中均可见浸润细胞的凋亡 ,虹膜睫状体中凋亡细胞明显多于脉络膜和视网膜中的凋亡细胞。 结论 单核巨噬细胞、淋巴细胞和 MHC- +细胞均参与了 EAU的形成 ,在 EAU早期即有浸润细胞发生凋亡 ,此可能是导致这种炎症迅速消退的重要机制。 | 杨培增 Nicole H Herzberg 周红颜 黄祥坤 谢楚芳 金浩丽 籍利 Lidy Broersma Mavc D de Smet Aize Kijlstra | 2000 | 中华眼底病杂志2000，16，1： | 9 |
| 4 | Role of T-cell receptor V beta 8.3 peptide vaccine in the prevention of experimental autoimmune uveoretinitis显示文摘Background T-cell receptor (TCR) plays an important role in the development of autoimmune diseases. Recently, it was reported that immunization of animals with TCR peptide derived from the pathogenic cells could prevent autoimmune diseases. The aim of this study was to investigate whether vaccination with a synthetic peptide from the hypervariable region of TCR Vβ 8.3, an experimental autoimmune uveoretinitis (EAU)-associated gene, was able to prevent the disease. Methods EAU was induced in Lewis rats by immunization with IRBP R16 peptide emulsified in complete Freund’s adjuvant (CFA). The clinical and histological appearances were scored. Delayed type hypersensitivity (DTH) and lymphocyte proliferation were detected. Cytokine levels of aqueous humour, supernatants of cells from spleen and draining lymph nodes were measured by enzyme linked immunosorbent assay (ELISA). Gene expression of TCR Vβ 8.3 on CD4+ T cells was examined by real time quantitative polymerase chain reaction (PCR). Results After vaccination, the intraocular inflammation was significantly mitigated, antigen specific DTH and lymphocyte proliferation responses were suppressed, interleukin (IL)-2 in aqueous humour, interferon (IFN)-( and IL-2 produced by the spleen and draining lymph node cells were significantly decreased, whereas the production of IL-4 and IL-10 were increased. The response of draining lymph node cells to TCR Vβ 8.3 peptide was enhanced after vaccination. Inoculation with CFA alone did not affect the severity of EAU and the above parameters. The suppression of EAU was much stronger in the group of four fold inoculations than the group of two fold inoculations. The expression of TCR Vβ 8.3 gene was significantly reduced in the group of fourfold inoculations. Conclusion Vaccination with the synthetic TCR Vβ 8.3 peptide could remarkably inhibit the development of EAU. | ZHANG Rui YANG Pei-zeng WU Chang-you JIN Hao-li LI Bing HUANG Xiang-kun ZHOU Hong-yan GAO Yang ZHU Lian-xiang Aize Kijlstra | 2006 | Chinese Medical Journal2006，，9： | 8 |
| 5 | The Optimization of Ultrasonic Extraction Technology of Total Flavonoids in Leaves of Mallotus apelta by Response Surface Analysis Methodology显示文摘[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves. | Dengfeng ZOU Ruifen FAN Xiao HUANG Aize XIE | 2018 | Medicinal Plant2018，9，4： | 8 |
| 6 | Extraction Process Optimization of Total Flavonoids from Mallotus apelta Stems by Central Composite Design/Response Surface Method显示文摘[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good. | Xiao HUANG Dengfeng ZOU Ruifen FAN Shuoying GUO Hua ZHU Aize XIE | 2017 | Medicinal Plant2017，8，5： | 2 |
| 7 | Robotic fiber placement process analysis and optimization using response surface method显示文摘 | AIZED T SHIRINZADEH B | 2011 | International Journal of Advanced Manufacturing Technology2011，55，14： | 1 |
| 8 | 内毒素在Lewis鼠诱导的角膜改变——角膜平片的免疫组织化学研究显示文摘目的:探讨内毒素全身注射所致的角膜改变。方法:使用抗单核细胞、巨噬细胞和MHC-Ⅱ类抗原阳性细胞的单克隆抗体,用标准的ABC方法于内毒素注射前、后制备的角膜平片上进行免疫组织化学染色。结果:发现在正常角膜中央区实质内有散在分布的巨噬细胞,越近角膜缘分布越密集,MHC-Ⅱ类抗原阳性细胞则仅存在于角膜缘;内毒素注射后,中央区角膜单核巨噬细胞增多,这些细胞于角膜实质内发生了一系列形态学改变,MHC-Ⅱ类抗原阳性细胞仅见于注射后早期的中央区角膜内皮面。结论:内毒素诱导的角膜中巨噬细胞的增多可能是机体应激状态下的重要防御机制,角膜实质中MHC-Ⅱ类抗原的缺如则对维持角膜局部免疫微环境的稳定性有重要意义。眼科学报1996;12:70—74。 | 杨培增 Alex F de Vos Aize Kijlstra | 1996 | Eye Science1996，16，2： | 1 |
| 9 | A wind tunnel study of airflow through horticultural crops:determination of the drag coefficient显示文摘 | Molina Aiz F D Valera D L Alvarez A J | 2006 | Biosystems Enginee-ring2006，93，4： | 1 |
| 10 | Immunohistochemical Studies of Macrophages and MHC Class Ⅱ -positive Cells in the Iris and Ciliary Body of Lewis Rats显示文摘Purpose:To investigate the density, distribution and morphology of macrophages and MHC class II -positive dendritic cells in the iris and ciliary body of lewis rats. Methods:Immunohistochemistry was performed using monoclonal antibodies specific to monocytes and macrophages (ED1,ED2) and MHC class II -positive cells (OX6) on wholemounts of the iris-ciliary body complex isolated form normal lewis rats.Results:A well developed network of macrophages was present in the iris and ciliary body of normal lewis rats. These cells, morphologically displaying dendriti-form or pleiomorphic appearance, were more densely arranged in mid-iris (950 + 189 cells/mm2) than in iris base (482 ± 78 cells/mm2) and pupil margin (595 ± 92 cells/mm2). A similar network of MHC class II -positive cells with a cell density 452 ± 78 cells/mm2 was almost uniformly distributed in the iris of normal lewis rats.Conclusions : A network of macrophages and MHC class II -positive cells was established in the iris and ciliary body of | Alex F.de Vos Aize Kijlstra | 1996 | Eye Science1996，16，1： | 1 |
| 11 | Cross-linkable ftuoriated poly (ether ether ketene) polymers For optical waveguide devices显示文摘 | Zhao Yu Wang Fei Li Aize etal | 2004 | Mater Lett2004，58，21： | 1 |
| 12 | A New Cluster Validity for Data Clustering显示文摘 | Xulei Yang Qing Songl Aize Cao | 2006 | Neural Processing Letters2006，，23： | 1 |
| 13 | Generalization of OWAVEC method for simultaneous noise suppression, data compression and orthogonal signal correction显示文摘 | D'lez IE S' aiz JMG Pizarro C | 2005 | Anal Chim Acta2005，544，12： | 1 |
| 14 | OWAVEC: a combination of wavelet analysis and an orthogonalization algorithm as a preprocessing step in multivariate calibration显示文摘 | D'lez IE S'aiz J M G Pizarro C | 2004 | Anal Chim Acta2004，515，1： | 1 |
| 15 | IL-23 promotes CD4 + T cells to produce IL-17 in Vogt-Koyanagi-Harada disease显示文摘 | Wei Chi Peizeng Yang Bing Li Changyou Wu Haoli Jin Xuefei Zhu Lina Chen Hongyan Zhou Xiangkun Huang Aize Kijlstra | 2007 | The Journal of Allergy and Clinical Immunology2007，，5： | 1 |
| 16 | Cross-linkable fluoriated poly(ether ether ketone)polymers for optical waveguide devices显示文摘 | Yu Zhao Fei Wang Aize li | 2004 | Mater Lett2004，58，： | 1 |
| 17 | Lutein: More than just a filter for blue light显示文摘 | Aize Kijlstra Yuan Tian Elton R. Kelly Tos T.J.M. Berendschot | 2012 | Progress in Retinal and Eye Research2012，，4： | 1 |
| 18 | Mixture resolution according to the percentage of robusta variety in order to detect adulteration in roasted coffee by near infrared spectroscopy显示文摘 | Pizarro C Esteban-D'rez I Gonzalez-S'aiz JM | | 0，，2： | 1 |
| 19 | A wind tunnel study of airflow through horticultural crops: Determi-nation of the Drag Coefficient显示文摘 | Molina Aiz F D Valera D L Alvarez A J | 2006 | Biosystems Engineering2006，93，4： | 1 |
| 20 | Robotic fiber placement process analysis and optimization using response surface method显示文摘 | Aized T Shirinzadeh B | 2011 | The Internation- al Journal of Advanced Manufacturing Technology2011，55，39： | 1 |
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