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| 1 | Molecular Characterization of Viral G Gene in Emerging and Re-emerging Areas of Rabies in China, 2007 to 2011显示文摘In recent years (2007 to 2011), although the overall number of rabies cases in China has decreased, there is evidence of emerging or re-emerging cases in regions without previous rabies cases or with low incidence of rabies. To investigate the origin and the factors affecting the spread of rabies in China, specimens were collected from 2007 to 2011 from provinces with emerging and re-emerging cases and tested for the presence of the rabies virus. Positive specimens were combined with sequences from GenBank to perform comparisons of homology and functional sites, and to carry out phylogenetic analyses. Out of these regions, five provinces had 9 positive specimens from canine and cattle, and 34 canine or human specimens were obtained from previously high-incidence provinces. Complete sequences of G gene were obtained for these samples. Homology of the sequences of these 43 specimens was 87%-100% at the nucleotide level and 93.7% -100% at the amino acid level. These G gene sequences were combined with reference sequence from GenBank and used to construct a phylogenetic tree. The results showed that 43 specimens were all assigned to China clade I and clade II, with all specimens from emerging and re-emerging areas placed within clade I. Specimens isolated from Shanxi and Inner Mongolia in 2011 were distinct from previously-isolated local strains and had closer homology to strains from Hebei, Beijing and Tianjin whereas new isolates from Shanghai were tightly clustered with strains isolated in the 1990s. Finally, Shaanxi isolates were clustered with strains from adjacent Sichuan. Our results suggest that the rabies cases in emerging and re-emerging areas in China in the last 5 years are a consequence of the epidemic spreading from of neighboring provinces and regions experiencing a serious epidemic of rabies. | Shu-LinLang Xiao—YanTao Zhen-YangGuo QingTang HaoLi Cui.PingYin YingLi Guo—DongLiang | 2012 | Virologica Sinica2012，27，3： | 18 |
| 2 | The Full-length Genome Analysis of a Street Rabies Virus Strain Isolated in Yunnan Province of China显示文摘The epidemic of rabies has rapidly increased and expanded in Yunnan province in recent years. In order to further analyze and understand the etiological reasons for the rapid expansion of rabies in Yunnan, a strain of rabies virus CYN1009D in Yunnan was isolated, and the complete genomic sequencing was carried out, and the bioimfomative analysis on genes/encoded proteins and phylogeny with reference to sequences in GenBank was performed. The complete genome of CYN1009D was 11923 nt in length and belonged to genotype I. The genes encoding different structural proteins were all conserved in their lengths, in comparison to other strains in China. The amino acid sequence was conserved at different antigen sites of NP, but the variation was detected at the secondary phosphorylation site of position 375; variations were also detected in the phosphorylation sites at positions 63-63 and 162 of PP; the sites playing important roles in virus synthesis, budding and viral morphology in MP were conserved; two glycosylation sites were detected at Asn37 and Asn319 in GP, the neutralizing antigen sites in GP were conserved; the initial amino acid of LP (ML) was different from that of most of the strains in China (MM); the variations in G-L region in the intergenic region were significant. The phylogenic tree showed that CYN1009D has a closer genetic relationship to the strains in Southeast Asia, indicating that prevention and control on rabies in borderland areas should be reinforced meanwhile efforts are made to control rabies in China. | JianZhang Hai—linZhang Xiao-yanTao HaoLi QingTang | 2012 | Virologica Sinica2012，27，3： | 11 |
| 3 | Preparation of fine copper powders and their application in BME-MLCC显示文摘The preparation of fine copper powders by chemical reduction method was investigated. The reaction of [Cu(NH3)4] 2+ complex with hydrazine hydrate gives spherical monodispersed fine copper powders. The spherical copper powder with a uniform size of 3.5 ± 0.5 μm was processed to obtain flake copper powder having a uniform size of 8-10 μm, excellent dispersibility and uniform shape. The spherical copper powder of 2.5 ± 0.3 μm in size, flake copper, glass frit and vehicle were mixed to prepare copper paste, which was fired in 910-920°C to obtain BME-MLCC (base metal multilayer ceramic capacitor) with a dense surface of end termination, high adhesion and qualified electrical behavior. Polarized light photo and SEM were employed to observe the copper end termination of BME-MLCC. The rough interface from the interfacial reaction between glass and chip gives high adhesion. | Songping Wu Haoli Qin Pu Li | 2006 | Journal of University of Science and Technology Beijing2006，13，3： | 6 |
| 4 | Protein kinase C-dependent activation of P44/42 mitogen-activated protein kinase and heat shock protein 70 in signal transduction during hepatocyte ischemic preconditioning显示文摘AIM: To investigate the significance of protein kinase C (PKC), P44/42 mitogen-activated protein kinase (MAPKs) and heat shock protein (HSP)70 signal transduction during hepatocyte ischemic preconditioning. METHODS: In this study we used an in vitro ischemic preconditioning (IP) model for hepatocytes and an in vivo model for rat liver to investigate the significance of protein kinase C (PKC), P44/42 mitogen-activated protein kinase (P44/42 MAPKs) and heat shock protein 70 (HSP70) signal transduction in IP. Through a normal liver cell hypoxic preconditioning (HP) model in which cultured normal liver cells were subjected to 3 cycles of 5 rain of incubation under hypoxic conditions followed by 5 rain of reoxygenation and subsequently exposed to hypoxia and reoxygenation for 6 h and 9 h respectively. PKC inhibitor, activator and MEK inhibitor were utilized to analyze the phosphorylation of PKC, the expression of P44/42 MAPKs and HSP70. Viability and cellular ultrastructure were also observed. By using rat liver as an in vivo model of liver preconditioning (3 cycles of 10-min occlusion and 10-min reperfusion), in vivo phosphorylation of PKC and P44/42MAPKs, HSP70 expression were further analyzed. AST/ALT concentration, cellular structure and ultrastruture were also observed. All the data were statistically analyzed. RESULTS: Similar results were obtained in both in vivo and in vitro IP models. Compared with the control withouts IP (or HP), the phosphorylation of PKC and P44/42 MAPKs and the expression of HSP70 were obviously increased in IP (or HP) treated model in which cytoprotection could be found. The effects of preconditioning were mimicked by stimulating PKC with 4β phorobol-12-myristate 13-acetate (PMA). Conversely, inhibiting PKC with chelerythrine abolished the protection given by preconditioning. PD98059, inhibitor of MEK (the upstream kinase of P44/42MAPKs), also reverted the cytoprotection exerted by preconditioning. CONCLUSION: The results demonstrate that preconditioning induces a rapid activation of P44/421VlAPKs and PKC activation plays a pivotal role in the activation of P44/42 MAPKs pathway that participates in the preservation of liver cells. HSP expression is regulated by signals in PKC dependent P44/42 MAPKs pathway. | YiGao Yu-QiangShan Ming-XinPan YuWang Li-JunTang HaoLi ZhiZhang | 2004 | World Journal of Gastroenterology2004，10，7： | 5 |
| 5 | Preparation of nitrogen-doped titania and its photocatalytic activity显示文摘Yellowish nitrogen-doped titania was produced through sol-gel method in mild condition, with the elemental ni- trogen derived from aqua ammonia. The titania catalysts were characterized using XRD, BET, TEM, XPS, and UV-Vis diffuse reflectance spectrophotometer, and their photocatalytic activities were evaluated under UV and visible light, respec- tively. The XRD results showed that all titania catalysts were anatase. More significantly, the crystallite size of nitro- gen-doped titania increased with an increase in N/Ti proportion, and the doping of nitrogen could extend the absorption shoulder into the visible-light region, thus it possessed a higher visible-light activity illustrated by decolorization of methyl orange (65.3%) under the irradiation of visible light, whereas pure titania showed little of such kind of visible light activity. The UV-light activity of nitrogen-doped titania catalysts was worse than that of pure titania and Degussa P25. In the range of N/Ti proportion of 4-10 mol%, the activity of nitrogen-doped titania weakened appreciably in the visible-light region as the N/Ti proportion increased, whereas a reverse relationship existed under the irradiation of UV light. | QIN Haoli GU Guobang LIU Song | 2007 | Rare Metals2007，26，3： | 5 |
| 6 | Effects of mycorrhizal fungus Mycena sp. on the growth and poly-saccharide properties of Dendrobium officinale显示文摘Dear Editor,Dendrobium officinale Kimura et Migo(Orchidaceae),an endangered species,is a medicinal plant and the sole origin plant of Dendrobii Officinalis Caulis(Tiepishihu),a rare Chinese herbal medicine.Several photosynthetic | Xiaomei Chen Haoli Yan Chunlan Wang Lixia Tian Airong Wang Shunxing Guo | 2016 | Science China(Life Sciences)2016，59，9： | 2 |
| 7 | Magnetic gold-nanorod/PNIPAAmMA nanoparticles for dual magnetic and photoacoustic imaging and targeted photothermal therapy显示文摘 | Yang Hungwei Liu Haoli Li Menglin | 2013 | Biomaterials2013，4，22： | 1 |
| 8 | Influence of three -dimensional wall rough- ness on the laminar flow in microtube显示文摘 | Haoli Wang Yuan Wang | 2007 | International Journal of Heat and Fluid Flow2007，，28： | 1 |
| 9 | Measurement of water flow rate in microchannels based n the microfluidic particle image velocimetry 显示文摘 | Wang Haoli Wang Yuan | 2009 | Measurement2009，42，： | 1 |
| 10 | Magnetic properties and magnetization reversal of alpha Fe nanowires deposited in alumina film显示文摘 | Yong Peng Zhang Haoli Pan Shanlin | 2000 | J Appl Phys2000，87，10： | 1 |
| 11 | Flow in the microchannel with rough wall: flow pattern and pressure drop 显示文摘 | Wang Haoli Wang Yuan | 2007 | Journal of Micromechanics and Microengineering2007，17，3： | 1 |
| 12 | Nitrogen‐Incorporated TS‐1 Zeolite Obtained by Post‐Synthetic Nitridation显示文摘 | HaoLi QianLei XiaomingZhang JishuanSuo | 2011 | ChemCatChem2011，，1： | 1 |
| 13 | Influence of three-dimensional wall roughness on the laminar flow in microtube显示文摘 | Wang Haoli Wang Yuan | 2007 | Heat and Fluid Flow2007，28，2： | 1 |
| 14 | On the Vertex-distinguishing Proper Edge-coloring of Graphs显示文摘 | BAZGAN C HARKAT-BENHAMDINE A HAOLi | 1999 | Journal of Combin Theory SerB1999，75，： | 1 |
| 15 | Separation of acetone, butanol and ethanol(ABE) from dilute aqueous solutions by silicalite-1/PDMS hybrid pervaporation membranes显示文摘 | Haoli Zhou Yi Su Xiangrong Chen | 2011 | Separation and Purification Technology2011，79，： | 1 |
| 16 | IL-23 promotes CD4 + T cells to produce IL-17 in Vogt-Koyanagi-Harada disease显示文摘 | Wei Chi Peizeng Yang Bing Li Changyou Wu Haoli Jin Xuefei Zhu Lina Chen Hongyan Zhou Xiangkun Huang Aize Kijlstra | 2007 | The Journal of Allergy and Clinical Immunology2007，，5： | 1 |
| 17 | Separation of acetone, butanol and ethanol (ABE) from dilute aqueous solutions by silicalite-1/PDMS hybrid pervaporation membranes显示文摘 | Haoli Zhou Yi Su Xiangrong Chen Yinhua Wan | 2011 | Separation and Purification Technology2011，，3： | 1 |
| 18 | Flow in microchannels with rough walls:flow pattern and pressure drop显示文摘 | Wang Haoli Wang Yuan | 2007 | Journal of Micromechanics and Microengineering2007，17，3： | 1 |
| 19 | Influence of three-dimensional wall roughness on the laminar flow in microtube显示文摘 | Wang Haoli Wang Yuan | 2007 | International Journal of Heat and Fluid Flow2007，28，2： | 1 |
| 20 | Phase separation of an acetone- butanol-ethanol (ABE)-water mixture in the permeate during pervaporation of a dilute ABE solution显示文摘 | ZHOU Haoli SU Yi WAN Yinhua | 2014 | Separation and Purification Technology2014，132，： | 1 |
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