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| 1 | The Optimization of Ultrasonic Extraction Technology of Total Flavonoids in Leaves of Mallotus apelta by Response Surface Analysis Methodology显示文摘[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves. | Dengfeng ZOU Ruifen FAN Xiao HUANG Aize XIE | 2018 | Medicinal Plant2018，9，4： | 8 |
| 2 | Study on Extraction and Separation of Dihydromyricetin from Vine Tea [Ampelopsis grossedentata(Hand-Mazz) W. T. wang]显示文摘Ampelopsis grossedentata(Hand-Mazz) W. T. wang is a typical edible plant with important physiological health functions. Dihydromyricetin is the main active ingredient of A. grossedentata, which has good pharmacological effects such as antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and liver protecting effects. This paper summarized the research progress on the extraction materials, extraction methods and separation and purification processes of dihydromyricetin in A. grossedentata to understand the best experimental method of dihydromyricetin, aiming to provide an important theoretical basis for the comprehensive development and utilization of dihydromyricetin. | Zhen KE Hua ZHU Shian ZHONG Dengfeng ZOU | 2020 | Agricultural Biotechnology2020，9，1： | 3 |
| 3 | Extraction Process Optimization of Total Flavonoids from Mallotus apelta Stems by Central Composite Design/Response Surface Method显示文摘[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good. | Xiao HUANG Dengfeng ZOU Ruifen FAN Shuoying GUO Hua ZHU Aize XIE | 2017 | Medicinal Plant2017，8，5： | 2 |
| 4 | Extraction Process and Content Determination of Total Flavonoids in Ginkgo( Ginkgo biloba L. ) Leaves显示文摘[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavonoids in ginkgo leaves were studied,and the optimal extraction conditions for flavonoids were determined by orthogonal test; and with quercetin as reference substance,total flavonoid content in ginkgo leaves was determined by UV spectrophotometry.[Results]The optimal extraction process was 4 h of Soxhlet extraction with methanol; and the total flavonoid contents had a good linear relation in the range of 0. 006 5-0. 039 mg/ml( R^2= 0. 999 9),the average content was stabilized at 1. 135%,and the average recovery of the method was 102. 0%. [Conclusions]This study selected the optimal extraction process for total flavonoids in ginkgo leaves. The test method is simple with high accuracy and precision,and is suitable for the extraction and determination of total flavonoids in ginkgo leaves. | Dengfeng ZOU Hua ZHU Binghua QIN Xiaohua WANG | 2019 | Agricultural Biotechnology2019，8，1： | 2 |
| 5 | Content Determination of Quercetin in Ferment of Ginkgo biloba L. Leaves by HPLC显示文摘[Objectives] This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the content of quercetin in ferment of G.biloba leaves was determined by reversed-phase high-performance liquid chromatography.First,the flavonoid glycosides were extracted with methanol.Then,the flavonoid glycosides were hydrolyzed with hydrochloric acid to prepare the test solution.The chromatographic conditions were as follows:Platisil ODS C_(18) column(150 mm × 4.6 mm,5 μm);V_(methonal)∶V_(water)(0.4% phosphoric acid solution) =55∶45;flow rate of 1 m L/min;Shimadzu UV detector;detection wavelength of 360 nm.[Results] Quercetin was used as a reference substance.In the range of 0.002 6-0.036 0 g/L,there was a good linear relationship,with correlation coefficient of 0.999 8 and RSD of 1.26%.[Conclusions] This method is simple,easy to operate,accurate,and reproducible.It is suitable for the determination of quercetin content in G.biloba leaves. | Dengfeng ZOU Hua ZHU Junying RUAN Xiaohua WANG | 2018 | Medicinal Plant2018，9，6： | 1 |
| 6 | Er doped BaBi 4 Ti 4 O 15 multifunctional ferroelectrics: Up-conversion photoluminescence, dielectric and ferroelectric properties显示文摘 | Dengfeng Peng Hua Zou Chaonan Xu Xusheng Wang Xi Yao | 2013 | Journal of Alloys and Compounds2013，，： | 1 |
| 7 | Photoluminescence and thermal stability of yellow-emitting Pr^(3+) doped CaAl_(2)O_(4) phosphors显示文摘Pr^(3+)-doped CaAl_(2)O_(4) phosphors with broad yellow emission were synthesized by conventional solid-state reaction in air and their structural and luminescent properties were investigated.A pure monoclinic structure can be obtained when the sintering temperature is 1400℃ or above.With photoluminescence(PL)measurement,the excitation was observed at 450–500 nm,which covered the emission of the blue light-emitting diode(LED)chips.Emission of the phosphors showed a green band and a red band.So these phosphors showed great potential in application of warm white LEDs without reabsorption.Additionally,the optimal emission intensity was obtained when Pr doping level was at 0.2 mol%.Furthermore,the Pr-doped CaAl_(2)O_(4) phosphors have the higher quenching temperature.With an increase in temperature,the emission bands of Pr-doped CaAl_(2)O_(4) showed an abnormal blue-shift. | Hua Zou Dengfeng Peng Zhenming Chu Xusheng Wang Yanxia Li Jun-Wei Hou Xi Yao | 2013 | Journal of Advanced Dielectrics2013，3，3： | 1 |
| 8 | The relief of hypoxic microenvironment using an O_(2) self-sufficient fluorinated nanoplatform for enhanced photodynamic eradication of bacterial biofilms显示文摘Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of PDT.In order to address this issue,fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial biofilms.The liposomes(denoted as Lip-Ce6-PFH@O_(2))were prepared by co-encapsulation of O_(2) carrier perfluorohexane(PFH)and photosensitizer chlorin e6(Ce_(6)).Lip-Ce6-PFH@O_(2) could achieve efficient biofilm penetration due to the positively charged surface.The hypoxic microenvironment of biofilms would then be relieved,leading to the generation of more ROS under laser irradiation.Therefore,the bactericidal capability of PDT could be significantly improved because of the co-delivered O_(2) carrier PFH.Lip-Ce6-PFH@O_(2) exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in vivo.Meanwhile,Lip-Ce6-PFH@O_(2) also effectively alleviated inflammation symptoms and accelerated wound healing in the mice model.In general,this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication. | Lingyun Zou Dengfeng Hu Fanjin Wang Qiao Jin Jian Ji | 2022 | Nano Research2022，15，2： | 0 |
| 9 | Study on the Photodynamic Efficiency of Sodium Chlorophyllin from Spirulina显示文摘[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy. | Jianjiao CHEN Zhiqiang CHEN Jun WEN Jian WEN Jingfei FENG Jing YI Dengfeng ZOU | 2021 | Agricultural Biotechnology2021，10，1： | 0 |
| 10 | Up-conversion luminescence of Er^(3+)and Yb^(3+)co-doped CaBi_(2)Ta_(2)O_(9) multifunctional ferroelectrics显示文摘Er^(3+)and Yb^(3+)co-doped CaBi_(2)Ta_(2)O_(9)(CBT)-based bismuth layered-structure oxides were synthesized by a simple solid-state reaction method.Their up-conversion(UC)luminescence,dielectric and ferroelectric properties were investigated.Two strong green emission bands centered at 526 and 547nm and a weak red emission band centered at 658nm were obtained under a 980nm laser excitation at room temperature.These emission bands originated from the radiative relaxation of Er^(3+)from 2H_(11)=2,4S_(3)=2,and 4F_(9/2) levels to the ground state 4I_(15)=2,respectively.At the meantime,the fluorescence intensity ratio(FIR)variation of two green UC emissions at 526 and 547nm has been studied as a function of temperature in the range of 153–603K.The maximum sensor sensitivity obtained was 39×10^(-4)K^(-1) at 590K,which indicated that Er^(3+)=Yb^(3+)co-doped CBT ceramic is a promising candidate for applications in optical high temperature sensor. | Qiufeng Cao Dengfeng Peng Hua Zou Jun Li Xusheng Wang Xi Yao | 2014 | Journal of Advanced Dielectrics2014，4，3： | 0 |
| 11 | Determination of the Content of Kaempferol from Fermented Ginkgo( Ginkgo biloba L.) Leaves显示文摘[Objectives] This study was conducted to determine kaempferol content in ginkgo( Ginkgo biloba L.) leaves subjected to microbial fermentation.[Methods]Bacillus licheniformis was selected for solid-state fermentation of ginkgo leaves,and the content of kaempferol in ginkgo leaves was determined by RPHPLC method. At first,methanol was used to extract flavonoid glycosides,which were then hydrolyzed by hydrochloric acid solution. HPLC was performed with Platisil ODS column C18( 150 mm ×4. 6 mm,5 μm) using mobile phase Vmethanol∶ Vwater( 0. 4% phosphoric acid solution) = 55∶45 at a flow rate of 1 ml/min,and the eluate was detected with a shimadzu HPLC ultraviolet detector at 360 nm. [Results]With kaempferol as the reference substance,the correlation coefficient was0. 999 2 in the range of 0. 001 06-0. 016 96 g/L. The content in the fermented product was less than that in the non-fermented product by 28%. [Conclusions]The method is simple,accurate,and is suitable for determination of kaempferol. This study will provide an experimental basis for the development and utilization of ginkgo. | Dengfeng ZOU Hua ZHU Bowen JIANG Xiaohua WANG | 2019 | Agricultural Biotechnology2019，8，1： | 0 |
| 12 | Functional genomics of Brassica napus:Progresses,challenges,and perspectives显示文摘Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil worldwide.Identification and understanding the gene functions in the B.napus genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B.napus.In this review,we present an overview of the progress made in the functional genomics of B.napus,including the availability of germplasm resources,omics databases and cloned functional genes.Based on the current progress,we also highlight the main challenges and perspectives in this field.The advances in the functional genomics of B.napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B.napus and will expedite the breeding of high quality,high resistance and high yield in B.napus varieties. | Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu Guangsheng Yang Tingdong Fu Liang Guo Hu Zhao | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
| 13 | HPLC Method for Determination of Content of Total Flavonoids from Ginkgo (Ginkgo biloba L.) Leaves显示文摘[Objectives]This study was conducted to determine the content of total flavonoids in ginkgo ( Ginkgo biloba L.) leaves.[Methods]The content of total flavonoids from ginkgo leaves was determined by reversed phase-high performance liquid chromatography (RP-HPLC).The flavonoid glycosides were first extracted with methanol,and hydrolyzed with hydrochloric acid solution to prepare a test solution.Platisil ODS C18 column (150 mm×4.6 mm,5 μm) and the mobile phase V methanol ∶ V water (0.4% phosphoric acid solution)=85∶ 15 were selected for HPLC separation.The HPLC separation was performed with the column at a column temperature of 25℃ using the mobile phase at a flow rate of 1 ml/min.The sample size was 10 μl,and detection was performed with an Agilent HPLC ultraviolet detector at 360 nm.[Results]The reference substance,quercetin,had good linearity in the range of 0.002 6-0.052 0 g/L,with a correlation coefficient of 0.999 7;and the RSD was 1.26%.[Conclusions]The determination method has rapid and simple operation with accurate results and is good in repeatability.This method is suitable for the determination of content of total flavonoids in ginkgo leaves. | Dengfeng ZOU Hua ZHU Zubo HUANG Xiaohua WANG | 2019 | Agricultural Biotechnology2019，8，3： | 0 |
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