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| 1 | Construction, expression and characterization of the engineered antibody against tumor surface antigen, p185^(c-erbB-2)显示文摘The c-erbB-2 proto-oncogene encodes a 185kDa protein p185, which belongs to epidermal growth factorreceptor family. Amplification of this gene has been shown to correlate with poor clinical prognosis forcertain cancer patients. The monoclonal antibody A21 which directed against p185 specifically inhibitsproliferation of tumor cells overexpressing p185, hence allows it to be a candidate for targeted therapy. Inorder to overcome several drawbacks of murine MAb, we cloned its VH and VL genes and constructed thesingle-chain Fv (scFv) through a peptide linker. The recombinant scFvA21 was expressed in Escherichiacoli and purified by the affinity column. Subsequently it was characterized by ELISA, Western blot, cellimmunohistochemistry and FACS. All these assays showed the binding activity to extracellular domain(ECD) of p185. Based on those properties of scFvA21, we further constructed the scFv-Fc fusion moleculewith a homodimer form and the recombinant product was expressed in mammalian cells. In a series ofsubsequent analysis this fusion protein showed identical antigen binding site and activity with the parentantibody. These anti-p185 engineered antibodies have promised to be further modified as a tumor targetingdrugs, with a view of application in the diagnosis and treatment of human breast cancer. | LIAN SHENG CHENG, AI PING LIU, JIA HONG YANG, YAN QIU DONG, LIANG WEI LI, JING WANG, CHAO CHEN WANG, JING LIUSchool of Life Science, University of Science and Technology of China, Hefei 230027, China | 2003 | Cell Research2003，13，1： | 24 |
| 2 | Recombinant protein subunit vaccine booster following two-dose inactivated vaccines dramatically enhanced anti-RBD responses and neutralizing titers against SARS-CoV-2 and Variants of Concern显示文摘Dear Editor,As of October,2021,SARS-CoV-2 has infected more than 230 million people;promoting roll-out vaccinations could help build herd immunity for the pandemic.However,the waning of antibody in magnitude and decreased protective efficacy in multiple types of vaccines have been reported. | Jingwen Ai Haocheng Zhang Qiran Zhang Yi Zhang Ke Lin Zhangfan Fu Jieyu Song Yuanhan Zhao Mingxiang Fan Hongyu Wang Chao Qiu Yang Zhou Wenhong Zhang | 2022 | Cell Research2022，32，1： | 19 |
| 3 | Soil Quality Assessment of Acid Sulfate Paddy Soils with Different Productivities in Guangdong Province,China显示文摘Land conversion is considered an effective measure to ensure national food security in China,but little information is available on the quality of low productivity soils,in particular those in acid sulfate soil regions.In our study,acid sulfate paddy soils were divided into soils with high,medium and low levels based on local rice productivity,and 60 soil samples were collected for analysis.Twenty soil variables including physical,chemical and biochemical properties were determined.Those variables that were significantly different between the high,medium and low productivity soils were selected for principal component analysis,and microbial biomass carbon(MBC),total nitrogen(TN),available silicon(ASi),pH and available zinc(AZn) were retained in the minimum data set(MDS).After scoring the MDS variables,they were integrated to calculate a soil quality index(SQI),and the high,medium and low productivity paddy soils received mean SQI scores of 0.95,0.83 and 0.60,respectively.Low productivity paddy soils showed worse soil quality,and a large discrepancy was observed between the low and high productivity paddy soils.Lower MBC,TN,ASi,pH and available K(AK) were considered as the primary limiting factors.Additionally,all the soil samples collected were rich in available P and AZn,but deficient in AK and ASi.The results suggest that soil AK and ASi deficiencies were the main limiting factors for all the studied acid sulfate paddy soil regions.The application of K and Si on a national basis and other sustainable management approaches are suggested to improve rice productivity,especially for low productivity paddy soils.Our results indicated that there is a large potential for increasing productivity and producing more cereals in acid sulfate paddy soil regions. | LIU Zhan-jun ZHOU Wei SHEN Jian-bo LI Shu-tian LIANG Guo-qing WANG Xiu-bin SUN Jing-wen AI Chao | 2014 | Journal of Integrative Agriculture2014，13，1： | 15 |
| 4 | Accelerating leaching of copper ore with surfactant and the analysis of reaction kinetics显示文摘To solve the problem of low permeability and lower extraction rates of high-mud ores, a surfactant was added as a penetrant to the pregnant leaching solution during column leaching tests. On the basis of the theories of physical chemistry and seepage flow mechanics, the mechanism by which seepage is enhanced under the effects of the surfactant was analyzed. The results show that the action modes of the surfactant were divided into four aspects: changing the wettability of the ore, reducing the viscosity of the leaching solution, adsorbing onto the surface of ore, and enhancing the permeability effect. The findings of column leaching tests demonstrated that permeability was substantially improved by the surfactant. In the later period of leaching, the permeability coefficient was two times higher than that of the control group. Meanwhile, the ore extraction rate increased by approximately 10%. During the leaching process, the surface tension of the solution did not substantially change, and that of the solution with surfactant increased slightly. The kinetics analysis of ore column leaching illustrated that the leaching processes were controlled by both internal diffusion(principal factor) and chemical reaction. | Chun-ming Ai Ping-ping Sun Ai-xiang Wu Xun Chen Chao Liu | 2019 | International Journal of Minerals,Metallurgy and Materials2019，26，3： | 9 |
| 5 | Prognosis of hepatic cirrhosis patients with esophageal or gastric variceal hemorrhage: multivariate analysis显示文摘Objective: To study the effect of bacterial infection,use of antibiotics, active bleeding at endoscopy, andthe severity of liver disease as prognostic factors inhepatic cirrhotic patients during the first 5 days afterthe episode of esophageal or gastric variceal hemor-rhage.Methods: Seventy-six hepatic cirrhosis patients withesophageal or gastric variceal bleeding were enrolled.Bleeding was managed in a standardized protocol u-sing octreotide and vasopressin in sclerotherapy orband ligation for active bleeding at endoscopy. Thescreening protocol for bacterial infection consisted ofchest radiograph; blood, urine and ascitic fluid cul-tures; the severity of liver disease shown by Child-Pugh score.Results: Active bleeding was observed at endoscopyin 40 patients (53%). Failure to control bleedingWithin 5 days occurred in 36 patients (45%). Empir-ical antibiotic treatment was used in 53 patients(67%), whereas bacterial infections were documen-ted in 43 patients (57%). Multivariate analysisshowed that proven bacterial infection (P<0.01) orantibiotic use (P<0.05) as well as active bleeding atendoscopy (P<0.01) and Child-Pugh score (P<0.01) were independent prognostic factors of failureto control bleeding.Conclusion: Bacterial infection is associated with fai-lure to control esophageal or gastric variceal bleedingin hepatic cirrhotic patients. | Chao Zhao Shao-Bo Chen Jian-Pin Zhou Wen Xiao Han-Gong Fan Xue-Wei Wu Gan-Xin Feng Wei-Xiong He the Department of Gatroenterology, Zhongshan Municipal Bo’ai Hospital, Zhongshan 528403, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，3： | 7 |
| 6 | CIVB flashback analysis of hydrogen flame based on azimuthal vorticity at mixing zone exit显示文摘Swirl-premixed combustion systems exhibit potential to meet future regulations on pollution emissions. However, combustion induced vortex breakdown(CIVB) flashback is frequently observed in these systems, especially for high hydrogen content fuel. In this study, a swirl-premixed burner with diverging centerbody was used to investigate CIVB flashback based on azimuthal vorticity at mixing zone exit. Through 2D axisymmetric model, it was found that there was a maximal azimuthal vorticity at mixing zone exit for each equivalence ratio. The physical meaning of these maximal azimuthal vorticity values was the minimally required azimuthal vorticity to trigger CIVB flashback. At the same time, the required azimuthal vorticity declined with the increase of equivalence ratio since turbulent burning velocity started to control flashback. Nevertheless, azimuthal vorticity offered by heat release increased with the increase of equivalence ratio, which promoted flame propagating upstream continually. | TIAN Xiao Jing XING Shuang Xi CUI Yu Feng FANG Ai Bing NIE Chao Qun | 2014 | Science China(Technological Sciences)2014，57，12： | 7 |
| 7 | Synthesis and application of a hydrophobic hypercrosslinked polymeric resin for removing VOCs from humid gas stream显示文摘恐水病的亢奋的 crosslinked 聚合树脂 LC-1 被准备并且描绘。LC-1 树脂的性质与商业亢奋的 crosslinked 聚合物 NDA-201 树脂的那些相比。另外, trichloroethylene (TCE ) 的动态吸附被调查到在在 303 K 的干燥、潮湿的条件下面的 LC-1 上, LC-1 拥有的结果表演高恐水病的性质和罐头把 TCE 从气体移开没有高湿度的效果,高效地流。 | Peng Liu Chao Long Hong Ming Qian Ying Li Ai Min Li Quan Xing Zhang | 2009 | Chinese Chemical Letters2009，20，4： | 7 |
| 8 | A new method for solving Biot's consolidation of a finite soil layer in the cylindrical coordinate system显示文摘一个新方法被开发在圆柱的坐标系统解决 Biot 有限土壤层的巩固。分别地,关于时间 t ,并列θ
和并列 r 基于 Biot 的巩固和 Laplace 变换, Fourier 扩大和 Hankel 变换的技术的管理方程排水量的一种关系,压力,过量毛孔水压力和流动在扎根的表面之间被建立( z = 0 )并且在 Laplace 和 Hankel 变换领域的任意的深度 z 。能被在变换域指有限土壤层,排水量的解决方案,压力,过量毛孔水压力和任何点的流动的合适的边界条件获得。在物理领域的实在溶液能被转换获得 Laplace 和 Hankel 转变。 | Zhiyong Ai Quansheng Wang Chao Wu | 2008 | Acta Mechanica Sinica2008，24，6： | 5 |
| 9 | Effect of Oxidation-Induced Material Parameter Variation on the High Temperature Oxidation Behavior of Nickel显示文摘In high temperature oxidation environment, the oxidation reaction will induce variations in material parameters, such as Young's modulus, thermal expansion coefficient(CTE),coefficient of oxygen diffusion(COD), etc. The oxidation-induced material parameter variations should be considered in high temperature mechanical analysis. In this paper, high temperature oxidation behavior of an oxide film/metal substrate system was investigated through a modified phase field approach. The oxidative stress and oxidation weight gain induced by high temperature oxidation were studied. Effects of Young's modulus, COD and CTE on oxidative stress in the oxide film were studied particularly. The simulation results showed that a better agreement with the experimental results could be obtained when considering the oxidation-induced material parameter variations in the high temperature mechanical analysis of oxide film/metal substrate system. The simulation results demonstrated that oxidative stress and oxidation weight gain were more sensitive to the variation of Young's modulus than to the variations of COD and CTE. | Chao Wang Shigang Ai Daining Fang | 2016 | Acta Mechanica Solida Sinica2016，29，4： | 5 |
| 10 | Pitting Kinetics of 304 Stainless Steel Using ESPI Detection Technique显示文摘The electronic speckle pattern interferometer was used to in situ monitor the pitting corrosion of 304 stainless steel at anodic polarization.The pitting current and pitting current density of a single pit were obtained.The pit growth was controlled by the corrosion products diffusion.The pit morphology was observed by a scanning electron microscope.The results showed that the pit was dish shaped,and the geometric parameters and pit growth time conformed to the function of Y = A+B1t+B2t2+B3t3. | Wen-Ming Tian Ying-Jun Ai Song-Mei Li Nan Du Chao Ye | 2015 | Acta Metallurgica Sinica(English Letters)2015，28，4： | 4 |
| 11 | An Aware-Scheduling Security Architecture with Priority-Equal Multi-Controller for SDN显示文摘Current SDN controllers suffer from a series of potential attacks. For example, malicious flow rules may lead to system disorder by introducing unexpected flow entries. In this paper, we propose Mcad-SA, an aware decision-making security architecture with multiple controllers, which could coordinate heterogeneous controllers internally as a 'big' controller. This architecture includes an additional plane, the scheduling plane, which consists of transponder, sensor, decider and scheduler. Meanwhile it achieves the functions of communicating, supervising and scheduling between data and control plane. In this framework, we adopt the vote results from the majority of controllers to determine valid flow rules distributed to switches. Besides, an aware dynamic scheduling(ADS) mechanism is devised in scheduler to intensify security of Mcad-SA further. Combined with perception, ADS takes advantage of heterogeneity and redundancy of controllers to enable the control plane operate in a dynamic, reliable and unsteady state, which results in significant difficulty of probing systems and executing attacks. Simulation results demonstrate the proposed methods indicate better security resilience over traditional architectures as they have lower failure probability when facing attacks. | Chao Qi Jiangxing Wu Guozhen Cheng Jianjian Ai Shuo Zhao | 2017 | China Communications2017，14，9： | 4 |
| 12 | Skeletal myogenesis by human embryonic stem cells显示文摘我们检验了在一个 xeno 移植动物的细胞建模的人的胚胎的茎(hES ) 的 myogenic 潜力。这里,我们证明区分开来与 hES 房间的先锋能在成年环境经历 myogenesis 并且在 myogenic 系产生房间类型的一个范围。这研究提供 hES 房间能在 vivo 改革肌肉和卫星房间并且是为对待肌肉的另一种有希望的房间类型的直接证据除了另外的 myogenic 房间类型的退化混乱。房间研究(2006 ) 16:713-722。做 i:10.1038/sj .cr.7310080;出版联机 2006 年 6 月 20 日。 | Jun Ke Zheng Yi Wang Aditi Karandikar Qian Wang Hui Gai Ai Lian Liu Chao Peng Hui Zhen Sheng | 2006 | Cell Research2006，16，8： | 4 |
| 13 | A super pan-genomic landscape of rice显示文摘Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity.Using de novo longread assembly,we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.Our pan-genome reveals extensive structural variations(SVs)and gene presence/absence variations.Additionally,our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter-and intraspecific diversity.Moreover,we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes.We characterized genetic variants associated with submergence tolerance,seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice.This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species. | Lianguang Shang Xiaoxia Li Huiying He Qiaoling Yuan Yanni Song Zhaoran Wei Hai Lin Min Hu Fengli Zhao Chao Zhang Yuhua Li Hongsheng Gao Tianyi Wang Xiangpei Liu Hong Zhang Ya Zhang Shuaimin Cao Xiaoman Yu Bintao Zhang Yong Zhang Yiqing Tan Mao Qin Cheng Ai Yingxue Yang Bin Zhang Zhiqiang Hu Hongru Wang Yang Lv Yuexing Wang Jie Ma Quan Wang Hongwei Lu Zhe Wu Shanlin Liu Zongyi Sun Hongliang Zhang Longbiao Guo Zichao Li Yongfeng Zhou Jiayang Li Zuofeng Zhu Guosheng Xiong Jue Ruan Qian Qian | 2022 | Cell Research2022，32，10： | 3 |
| 14 | Influence of fluvastatin on cardiac function and baroreflex sensitivity in diabetic rats显示文摘 | Fang XIE Chao SUN Li-hua SUN Jing-yuan LI Xin CHEN Hui CHE Guan-yi LU Bao-feng YANG Jing AI | 2011 | Acta Pharmacologica Sinica2011，32，3： | 3 |
| 15 | Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil显示文摘 | Chao Ai Guoqing Liang Jingwen Sun Xiubin Wang Ping He Wei Zhou | 2013 | Soil Biology and Biochemistry2013，，： | 2 |
| 16 | Towards a Dynamic Controller Scheduling-Timing Problem in Software-Defined Networking显示文摘Controller vulnerabilities allow malicious actors to disrupt or hijack the Software-Defined Networking. Traditionally, it is static mappings between the control plane and data plane. Adversaries have plenty of time to exploit the controller's vulnerabilities and launch attacks wisely. We tend to believe that dynamically altering such static mappings is a promising approach to alleviate this issue, since a moving target is difficult to be compromised even by skilled adversaries. It is critical to determine the right time to conduct scheduling and to balance the overhead afforded and the security levels guaranteed. Little previous work has been done to investigate the economical time in dynamic-scheduling controllers. In this paper, we take the first step to both theoretically and experimentally study the scheduling-timing problem in dynamic control plane. We model this problem as a renewal reward process and propose an optimal algorithm in deciding the right time to schedule with the objective of minimizing the long-term loss rate. In our experiments, simulations based on real network attack datasets are conducted and we demonstrate that our proposed algorithm outperforms given scheduling schemes. | Zhenping Lu Fucai Chen Guozhen Cheng Chao Qi Jianjian Ai | 2017 | China Communications2017，14，10： | 2 |
| 17 | Root stoichiometric dynamics and homeostasis of invasive species Phyllostachys edulis and native species Cunninghamia lanceolata in a subtropical forest in China显示文摘The community species abundance and diversity declined with bamboo invasion had been widely reported worldwide.However,the physiological strategies used during root competition between native species and invasive bamboo are poorly understood.To clarify the mechanisms underlying such strategies,the stoichiometric dynamics and homeostasis of nitrogen,phosphorus,organic carbon in root orders of Phyllostachys edulis(I du[=Ⅰ-Pe,years 1 and 2];Ⅱdu[=Ⅱ-Pe,years 3 and 4]),Cunninghamia lanceolata in transition and pure forests were analyzed.With increasing intensity of bamboo invasion,N,P,and C content of C.lanceolata root orders declined,N and P content in P.edulis rhizome orders declined,while C increased,the stoichiometric ratios in mixed forest interface mainly increased,and the stoichiometric differences within native and invasive species root orders narrowed.Meanwhile,the stoichiometric homeostasis index(H)of elements in the same root order and even the same elements in different root orders were not consistent.H of most root orders(except some HP)was greater than 4,the H ranked order wasⅠ-Pe>Cl>Ⅱ-Pe in mixed interfaces,and the N:P ratio of most species root orders was greater than 16,despite being affected by invasion.Our research concluded that the bamboo invasion narrows stoichiometric differences within root orders,and the juvenile bamboo rhizome has a stronger capacity for homeostatic regulation than in adult bamboo and C.lanceolata,which is a key determinant of bamboo invasion success. | Chao Peng Jia Tu Ming Yang Yong Meng Meiqun Li Wensheng Ai | 2021 | Journal of Forestry Research2021，32，5： | 2 |
| 18 | A phase-field study on the oxidation behavior of Ni considering heat conduction显示文摘Phase-field modeling approach has been used to study the oxidation behavior of pure Ni when considering heat conduction. In this calculation, the dependence of the coefficient of the Cahn–Hilliard equation Lc on the temperature T was considered. To this end, high-temperature oxidation experiments and phase-field modeling for pure Ni were performed in air under atmospheric pressure at 600,700, and 800?C. The oxidation rate was measured by thermogravimetry and Lc at these temperatures was determined via interactive algorithm. With the Lc-T relationship constructed, oxidation behavior of Ni when considering heat conduction was investigated. The influence of temperature boundaries on the oxidation degree, oxide film thickness, and specific weight gain were discussed. The phase-field modeling approach proposed in this study will give some highlights of the oxidation resistance analysis and cooling measures design of thermal protection materials. | Chao Wang Shigang Ai Daining Fang | 2016 | Acta Mechanica Sinica2016，32，5： | 2 |
| 19 | A systematic review of radiofrequency ablation versus percutaneous ethanol injection for small hepatocellular carcinoma up to 3 cm显示文摘 | Ai Shen Hua Zhang Chengyong Tang Yong Chen Yefei Wang Chao Zhang Zhongjun Wu | 2013 | J Gastroenterol Hepatol2013，，5： | 2 |
| 20 | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice显示文摘Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice.The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform.Eighty operational taxonomic unit were identified to be shared by all samples,and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves.The diversity of gut flora was slightly improved in diabetic mice after GFP treatment.The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP.The abundance of Robinsoniella,Flavonifractor,Anaerotruncus,and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis.Furthermore,Alloprevotella and Burkholderia had strong relevancy with other gut flora.Based on the findings,GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes. | Xiaoxiang Gao Dan Liu Luying Gao Yuezhen Ouyang Yuxi Wen Chao Ai Yuqing Chen Chao Zhao | 2022 | Food Science and Human Wellness2022，11，1： | 2 |
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