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| 1 | A new strawberry harvesting robot for elevated-trough culture显示文摘In order to improve robotic harvesting and reduce production cost,a harvesting robot system for strawberry on the elevated-trough culture was designed.It was supposed to serve for sightseeing agriculture and technological education.Based on the sonar-camera sensor,an autonomous navigation system of the harvesting robot was built to move along the trough lines independently.The mature fruits were recognized according to the H(Hue)and S(Saturation)color feature and the picking-point were located by the binocular-vision unit.A nondestructive end-effector,used to suck the fruit,hold and cut the fruit-stem,was designed to prevent pericarp damage and disease infection.A joint-type industrial manipulator with six degrees-of-freedom(DOF)was utilized to carry the end-effector.The key points and time steps for the collision-free and rapid motion of manipulator were planned.Experimental results showed that all the 100 mature strawberry targets were recognized automatically in the harvesting test.The success harvesting rate was 86%,and the success harvesting operation cost 31.3 seconds on average,including a single harvest operation of 10 seconds.The average error for fruit location was less than 4.6 mm. | Feng Qingchun Wang Xiu Zheng Wengang Qiu Quan Jiang Kai | 2012 | International Journal of Agricultural and Biological Engineering2012，5，2： | 8 |
| 2 | Numerical investigation on effects of side curtain opening behavior on indoor climate of naturally ventilated dairy buildings显示文摘The side-curtain is popular in cattle buildings to regulate indoor climates and ventilation rates by adjusting the opening ratio.It normally had three different adjusting strategies relate to the position of rollers,i.e.central roller(S1),top roller(S2)and bottom roller(S3),which result in different opening behaviors to generate the same opening ratio but different opening positions in the side wall for a full-curtain house.Numerical simulations were conducted using computational fluid dynamics(CFD)to investigate the effects of the eight potential opening behaviors of side curtains on the indoor climates and airflow rates in winter for a typical naturally ventilated dairy house in China when the opening ratio were 8.5%and 17%.Airflow patterns,wind chilled temperature(WCT)and age of air were analyzed in the animal occupied zone(AOZ)by taking reference planes.Openings at the very bottom of side walls had more efficient ventilation due to the younger air age,more effective air disturbing,more uniformly distributed indicators in AOZ.However,it will result in a lower WCT in AOZ although a lower ventilation rate was observed in this case.Openings on the very top of side wall would generate a better thermal comfort in AOZ but with very poor air quality and nonuniformly distributed airflows in the dairy house.S1 was not recommended to the practical application due to the poor indoor climate and the higher cost of the mechanical structure.Based on the comprehensive evaluations of the analytic hierarchy process,the most satisfaction opening positions were at the bottom of the side curtains and the optimized adjusting strategy is S2. | Qifeng Li Chuanxia Yao Luyu Ding Ligen Yu Weihong Ma Ronghua Gao Wengang Zheng | 2020 | International Journal of Agricultural and Biological Engineering2020，13，5： | 2 |
| 3 | Design and optimization on rootstock cutting mechanism of grafting robot for cucurbit显示文摘Cutting mechanisms in existing grafting machines are unable to completely cut through the rootstock growth point and can easily damage seedlings.During the mechanical operation of splice grafting,the cutting angle of the rootstock is an essential factor for ensuring the quality and survival rate of grafting seedlings and a stable process for grafting robots.Therefore,in this study,commonly used grafting rootstocks,e.g.,cucurbita moschata,and calabash gourd were used as research objects for studying and analyzing the cutting angle of a splice grafting method.The morphological and structural parameters of the rootstock and scion were measured,and a structural model of the internal cavity of the rootstock was constructed using an image analysis method.The critical cutting angles for the cucurbita moschata and calabash gourd seedlings were obtained.According to the analysis,the grafting cutting angles for cucumber seedlings matching with cucurbita moschata seedlings were 20°and 25°,respectively,and the fitting rate of the cutting surface of the rootstock and scion was 99.04%.A cutting mechanism for the rootstock growth point and geometric model of the cutting operation were established,and the structural parameters of the mechanism and cutting angle adjustment were optimized.A cutting performance test showed that the success rate of the pressing the cotyledons of cucurbita moschata seedlings was 96.67%,and the success rate of cutting was 98%.The cutting accuracy was 96.8%,and the cutting surface fitting rate of the rootstock and scion was 98.61%.The latter differed by 0.43%from the theoretical rate but met the requirements for the splice grafting method.Thus,this study can provide a reference for the design of a cutting mechanism for a grafting robot. | Kai Jiang Qian Zhang Liping Chen Wenzhong Guo Wengang Zheng | 2020 | International Journal of Agricultural and Biological Engineering2020，13，5： | 2 |
| 4 | Genetically modified CD7-targeting allogeneic CAR-T cell therapy with enhanced efficacy for relapsed/refractory CD7-positive hematological malignancies:a phase I clinical study显示文摘Chimeric antigen receptor(CAR)-T cell therapy against T cell malignancies faces major challenges including fratricide between CAR-T cells and product contamination from the blasts.Allogeneic CAR-T cells,generated from healthy donor T cells,can provide ready-to-use,blast-free therapeutic products,but their application could be complicated by graft-versus-host disease(GvHD)and host rejection.Here we developed healthy donor-derived,CD7-targeting CAR-T cells(RD13-01)with genetic modifications to resist fratricide,GvHD and allogeneic rejection,as well as to potentiate antitumor function.A phase I clinical trial(NCT04538599)was conducted with twelve patients recruited(eleven with T cell leukemia/lymphoma,and one with CD7-expressing acute myeloid leukemia).All patients achieved pre-set end points and eleven proceeded to efficacy evaluation.No dose-limiting toxicity,GvHD,immune effector cell-associated neurotoxicity or severe cytokine release syndrome(grade≥3)were observed.28 days post infusion,81.8%of patients(9/11)showed objective responses and the complete response rate was 63.6%(7/11,including the patient with AML).3 of the responding patients were bridged to allogeneic hematopoietic stem cell transplantation.With a median follow-up of 10.5 months,4 patients remained in complete remission.Cytomegalovirus(CMV)and/or Epstein-Barr virus(EBV)reactivation was observed in several patients,and one died from EBV-associated diffuse large B-cell lymphoma(DLBCL).Expansion of CD7-negative normal T cells was detected post infusion.In summary,we present the first report of a Phase I clinical trial using healthy donor-derived CD7-targeting allogeneic CAR-T cells to treat CD7^(+)hematological malignancies.Our results demonstrated the encouraging safety and efficacy profiles of the RD13-01 allogeneic CAR-T cells for CD7^(+)tumors. | Yongxian Hu Yali Zhou Mingming Zhang Houli Zhao Guoqing Wei Wengang Ge Qu Cui Qitian Mu Gong Chen Lu Han Tingting Guo Jiazhen Cui Xiaoyan Jiang Xiujun Zheng Shuhui Yu Xiaolong Li Xingwang Zhang Mingxi Chen Xiuju Li Ming Gao Kang Wang Cheng Zu Hao Zhang Xiaohong He Yanbin Wang Dongrui Wang Jiangtao Ren He Huang | 2022 | Cell Research2022，32，11： | 2 |
| 5 | Automatic diagnosis of strawberry water stress status based on machine vision显示文摘Water stress status of plants is very important for irrigation scheduling.However,plant water stress status monitoring has become the bottleneck of irrigation scheduling.In this study,an automatic water stress status monitoring method for strawberry plant was proposed and realized using combined RGB and infrared image information.RGB image and infrared images were obtained using RGB digital camera and infrared thermal camera,which were placed in a fixed shell in parallel.In the first experimental stage,three kinds of water stress treatments were carried out on three groups of strawberry plants,and each group includes three repetitions.Single point plant temperature,dry surface temperature,wet surface temperature were measured.In the second experimental stage,the infrared and visible light images of the canopy leaves were obtained.Meanwhile,plant temperature,dry surface temperature,wet surface temperature,and stomatal conductance were measured not only for single point but also for plant area temperature measurement.Fusion information of infrared image and visible light image was analyzed using image processing technology,to calculate the average temperature of plant areas.Based on single point temperature,area temperature,dry surface temperature and wet surface temperature of the plant,single point crop water stress index(CWSI)and area CWSI were calculated.Through analysis of variance(ANOVA),the experimental results showed that CWSI measured for plants under different treatments,were significantly different.Through correlation analysis,the experimental results showed that,determination coefficient between area CWSI and the corresponding stomatal conductance of three strawberry groups were 0.8834,0.8730 and 0.8851,respectively,which were larger than that of single-point CWSI and stomatal conductance.The results showed that area CWSI is more suitable to be used as the criteria for automatic diagnosis of plants. | Han Li Jian Yin Man Zhang Nick Sigrimis Yu Gao Wengang Zheng | 2019 | International Journal of Agricultural and Biological Engineering2019，12，1： | 1 |
| 6 | First time observation of sodium layer over Wuhan, China by sodium fluorescence lidar显示文摘 | Shunsheng Gong Xizhi Zeng Xinjian Xue Wengang Zheng Zhilin Hu Hanchun Jia Haizhi Zhang Yiping Liu | 1997 | Science in China Series A: Mathematics1997，，11： | 1 |
| 7 | Neural network and support vector machine models for the prediction of the liquefaction-induced uplift displacement of tunnels显示文摘Tunnels buried in liquefiable deposits are vulnerable to liquefaction-induced uplift damage during earthquakes.This paper presents support vector machine(SVM)and artificial neural network(ANN)models to predict the liquefaction-induced uplift displacement of tunnels based on artificial databases generated by the finite difference method.The performance of the SVM and ANN models was assessed using statistical parameters,including the coefficient of determination R^(2),the mean absolute error,and the root mean squared error.Applications for the above-mentioned approaches are compared and discussed.A relative importance analysis was adopted to quantify the sensitivity of each input variable.The precision of the presented models is demonstrated using centrifuge test results from previous studies. | Gang Zheng Wenbin Zhang Wengang Zhang Haizuo Zhou Pengbo Yang | 2021 | Underground Space2021，6，2： | 1 |
| 8 | Evaluation of the high-frequency capacitance sensor for measuring soil moisture显示文摘 | Cao Chun Xing Zhen Zheng Wengang | 2012 | Sensor Letters2012，10，2： | 1 |
| 9 | Tem- perature effect on fluorescence of PtOEP embedded in sol-gel membrane used in oxygen sensor显示文摘 | Xiande Zhao Wengang Zheng Daming Dong | 2013 | Optik-Inter- national Journal for Light and Electron Optics2013，124，24： | 1 |
| 10 | Grading method for tomato multi-view shape using machine vision显示文摘Owing to the requirements of a high yield and high-quality tomatoes, tomato grading is important-particularly for fruit morphology, and accuracy has become the focus of attention. Machine vision provides a fast and nondestructive manner to address this demand. In this study, the gamma correction method was used for preprocessing to enhance the edge information of tomatoes, and Otsu’s method was used to eliminate the tomato-image background in the A-component image under the LAB color model. On this basis, two levels of exploration were conducted. First, new evaluation indices were proposed for tomato shapes from different views. For the top view, two shape-evaluation indices were established: the area ratio of the maximum inscribed circle to the maximum circumscribed circle and the dispersion of the contour centroid distance (range and coefficient of variation), the highest accuracy was 94%. For the side view, the difference between the maximum and minimum centroid distances in the contour was established as a shape index, the highest accuracy was 91.91%. Second, an evaluation method based on multi-view fusion was developed by combining the advantage indices for different views. The classification accuracy reached 96%, with the highest identification accuracy of unqualified tomatoes. The results show that the proposed evaluation method combining top views (dispersion of centroid distance) with side views (difference between maximum and minimum centroid distances) is effective for classifying tomatoes. | Liping Chen Tingting He Zhiwei Li Wengang Zheng Shunwei An Lili ZhangZhong | 2023 | International Journal of Agricultural and Biological Engineering2023，16，6： | 0 |
| 11 | Design and experiment on scion cutting mechanism of grafting robot for cucurbit显示文摘The scion cutting mechanism of the traditional grafting machine cannot correct the bending degree of the scion seedling stem so that the cutting angle of the scion seedling is not standard and the survival rate of the grafted seedling is affected.In this study,cucumber seedlings and watermelon seedlings were taken as experimental objects.Based on the analysis of the geometric parameters and mechanical characteristics of scion seedlings,a scion cutting mechanism with holding seedling applied to cucumber seedlings and watermelon was designed and developed.The main structure and operating parameters of V-shaped holding seedling block and cutting unit were determined,and holding seedling and cutting performance tests were carried out.The test results showed that compared with the common arc clamp,the V-shaped holding seedling block had a better guiding and centering effect on the scion seedling,could better correct the bending state of the scion seedling stem,and the success rate of holding seedling of the V-shaped holding block reached 98.33%,which was increased by 13.33%compared with the arc-shaped clamp,and the seedling injury rate was only 1.67%.The upper and lower two-point positioning of scion stem by clamping hand and holding seedling block can improve the cutting performance effectively.The cutting accuracy rate of the scion was 97.75%,and the cutting success rate was 98.67%.The cutting performance was closely related to the holding seedling effect.The research results can provide a reference for the design of cutting mechanisms of melon grafting robots. | Liping Chen Kai Jiang Qian Zhang Wenzhong Guo Wengang Zheng | 2020 | International Journal of Agricultural and Biological Engineering2020，13，5： | 0 |
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